Human mast cells express androgen receptors but treatment with testosterone exerts no influence on IgE‐independent mast cell degranulation elicited by neuromuscular blocking agents
Abstract
Please cite this paper as: Human mast cells express androgen receptors but treatment with testosterone exerts no influence on IgE‐independent mast cell degranulation elicited by neuromuscular blocking agents. Experimental Dermatology 2010; 19: 302–304. Abstract: Women predominate in the anaphylactic reactions to neuromuscular blocking agents (NMBA). The expression of oestrogen receptors has been demonstrated in mast cells and oestrogen treatment can enhance mast cell degranulation, but the influence of androgens remains largely unclear. Our immunocytochemical study showed the expression of androgen receptor (AR) in mast cells isolated from human foreskin as well as in two human mast cell lines, HMC‐1 and LAD2. The amount of AR was most abundant in human skin mast cells as determined by real‐time polymerase chain reaction analysis. Treatment of the HMC‐1 mast cells with testosterone or 17β‐oestradiol, alone or in combination with different NMBA, did not affect mast cell degranulation as measured by the release of β‐hexosaminidase. Our study shows for the first time the expression of AR in human skin mast cells. Further studies using primary human mast cell cultures are needed to understand whether and how sex hormones can influence mast cell activation.
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“The role of female hormones, estradiol and progesterone, on MC behavior have been themost intensely studied, while very few recent studies have addressed the role of androgens [24, 29]; besides, majority of these studies have been done using MCs lines or freshly isolated rat PMCs or mouse bone marrowderived MC obtaine…”
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- Authors
- Chen, W., Beck, I., Schober, W., Brockow, K., Effner, R., Buters, J. T. M., Behrendt, H., & Ring, J.
- Affiliations
- Helmholtz Munich (DE)
- Technical University of Munich (DE)
- Corresponding author
- Chen, WenChieh
- Venue
- Experimental Dermatology
- Publisher
- Wiley
- Published
- 2010-02-11
- Volume
- 19
- Issue
- 3
- Pages
- 302-304
- ISSN
- 0906-6705 (ISSN-L)
- 1600-0625
- OpenAlex
- W2068512021
- Cited by
- 82
- References
- 19
- Citations by year
- 2026: 4 · 2025: 3 · 2024: 9 · 2023: 10 · 2022: 4 · 2021: 6 · 2020: 7 · 2019: 7 · 2018: 8 · 2017: 1 · 2016: 6 · 2015: 5 · 2014: 3 · 2013: 2 · 2012: 5
- FWCI
- 1.00
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Experimental Dermatology (publishedVersion)
- PubMed (publishedVersion)
- mediaTUM (Technical University of Munich) (submittedVersion)
- Site cant be reached (submittedVersion)
- Funders
- Alexander von Humboldt-Stiftung
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Mast cells and histamine
- Health Sciences › Medicine › Pharmacology › Drug-Induced Adverse Reactions
- Health Sciences › Medicine › Immunology and Allergy › Food Allergy and Anaphylaxis Research
- Keywords
- androgen receptor, mast cells, testosterone, neuromuscular blocking agents, anaphylaxis, sex hormones, oestrogen receptor, skin mast cells, HMC-1 cells, LAD2 cells, beta-hexosaminidase release
- MeSH terms
- Anaphylaxis, beta-N-Acetylhexosaminidases, Cell Line, Cells, Cultured, Estradiol, Female, Humans, Immunoglobulin E, Immunohistochemistry, Male, Mast Cells, Neuromuscular Blocking Agents, Receptors, Androgen, Sex Characteristics, Testosterone, Cell Degranulation, Gene Expression, Estrogen Receptor alpha, In Vitro Techniques
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