Anti-wrinkling effects of the mixture of vitamin C, vitamin E, pycnogenol and evening primrose oil, and molecular mechanisms on hairless mouse skin caused by chronic ultraviolet B irradiation
Abstract
BACKGROUND: Naturally occurring antioxidants were used to regulate the skin damage caused by ultraviolet (UV) radiation because several antioxidants have demonstrated that they can inhibit wrinkle formation through prevention of matrix metalloproteinases (MMPs) and/or increase of collagen synthesis. OBJECTIVE: We examined the effect of oral administration of the antioxidant mixture of vitamin C, vitamin E, pycnogenol, and evening primrose oil on UVB-induced wrinkle formation. In addition, we investigated the possible molecular mechanism of photoprotection against UVB through inhibition of collagen-degrading MMP activity or through enhancement of procollagen synthesis in mouse dorsal skin. METHODS: Female SKH-1 hairless mice were orally administrated the antioxidant mixture (test group) or vehicle (control group) for 10 weeks with UVB irradiation three times a week. The intensity of irradiation was gradually increased from 30 to 180 mJ/cm2. Microtopographic and histological assessment of the dorsal skins was carried out at the end of 10 weeks to evaluate wrinkle formation. Western blot analysis and EMSA were also carried out to investigate the changes in the balance of collagen synthesis and collagen degradation. RESULTS: Our antioxidant mixture significantly reduced UVB-induced wrinkle formation, accompanied by significant reduction of epidermal thickness, and UVB-induced hyperplasia, acanthosis, and hyperkeratosis. This antioxidant mixture significantly prevented the UVB-induced expressions of MMPs, mitogen-activated protein (MAP) kinase, and activation of activator protein (AP)-1 transcriptional factor in addition to enhanced type I procollagen and transforming growth factor-beta2 (TGF-beta2) expression. CONCLUSION: Oral administration of the antioxidant mixture significantly inhibited wrinkle formation caused by chronic UVB irradiation through significant inhibition of UVB-induced MMP activity accompanied by enhancement of collagen synthesis.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
A 10-week administration of an antioxidant mixture of pycnogenol, evening primrose oil, vitamin C and vitamin E to hairless mice irradiated three times a week with UVB radiation significantly prevented the UVB-induced expression of MMPs and mitogen-activated protein kinase, as well as the activation of the transcription factor AP-1.
“The study showed that a 10-week administration of the antioxidants to hairless mice irradiated three times a week with UVB radiation significantly prevented the UVB-induced expression of MMPs and mitogen-activated protein (MAP) kinase, as well as the activation of the transcription factor AP-1.”
From Bioactive Compounds for Skin Health: A Review (2021) · cites this paper as [203]
Raw polyphenolic materials exert synergistic effects with other antioxidants in preventing skin ageing.
“Raw polyphenolic materials exert synergistic effects with other antioxidants in preventing skin ageing.”
From Bioactive Compounds for Skin Health: A Review (2021) · cites this paper as [203]
The oral administration of the antioxidant mixture enhanced the expression of type I procollagen and TGF-β2.
“It also enhanced the expression of type I procollagen and TGF-β2).”
From Bioactive Compounds for Skin Health: A Review (2021) · cites this paper as [203]
The oral administration of the antioxidant mixture can inhibit wrinkle formation by preventing the expression of MMPs and increasing collagen synthesis.
“The results indicate that the oral administration of the antioxidant mixture can inhibit wrinkle formation by preventing the expression of MMPs and increasing collagen synthesis [203].”
From Bioactive Compounds for Skin Health: A Review (2021) · cites this paper as [203]
Cites
Opposing Effects of ERK and JNK-p38 MAP Kinases on Apoptosis
Xia, Z., Dickens, M., Raingeaud, J., Davis, R. J., & Greenberg, M. E. (1995) · Science · doi:10.1126/science.270.5240.1326 · Cited by 5,290 · see citing works
Molecular basis of sun-induced premature skin ageing and retinoid antagonism
Fisher, G. J., Datta, S. C., Talwar, H. S., Wang, Z.-Q., Varani, J., Kang, S., & Voorhees, J. J. (1996) · Nature · doi:10.1038/379335a0 · Cited by 1,410 · see citing works
Self-citationRohdewald, P. (2002) · International journal of clinical pharmacology and therapeutics · doi:10.5414/cpp40158 · Cited by 507 · see citing works
Natural phenolics in the prevention of UV-induced skin damage. A review
Svobodová, A. R., Psotová, J., & Daniela Walterová, B. (2003) · Biomedical Papers · doi:10.5507/bp.2003.019 · Cited by 452 · see citing works
Molecular mechanisms of retinoid actions in skin
Fisher, G. J., & Voorhees, J. J. (1996) · The FASEB Journal · doi:10.1096/fasebj.10.9.8801161 · Cited by 419 · see citing works
Reduced Type I and Type III Procollagens in Photodamaged Adult Human Skin
Talwar, H. S., Griffiths, C. E. M., Fisher, G. J., Hamilton, T. A., & Voorhees, J. J. (1995) · Journal of Investigative Dermatology · doi:10.1111/1523-1747.ep12318471 · Cited by 400 · see citing works
Fisher, G. J., Talwar, H. S., Lin, J., Lin, P., McPhillips, F., Wang, Z., Li, X., Wan, Y., Kang, S., & Voorhees, J. J. (1998) · Journal of Clinical Investigation · doi:10.1172/jci2153 · Cited by 392 · see citing works
Self-citationQuan, T., He, T., Kang, S., Voorhees, J. J., & Fisher, G. J. (2004) · American Journal Of Pathology · doi:10.1016/s0002-9440(10)63337-8 · Cited by 386 · see citing works
Self-citationChung, K. Y., Agarwal, A., Uitto, J., & Mauviel, A. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.6.3272 · Cited by 314 · see citing works
Chung, J. H. (2003) · Photodermatology Photoimmunology & Photomedicine · doi:10.1034/j.1600-0781.2003.00027.x · Cited by 269 · see citing works
Vayalil, P. K., Mittal, A., Hara, Y., Elmets, C. A., & Katiyar, S. K. (2004) · Journal of Investigative Dermatology · doi:10.1111/j.0022-202x.2004.22622.x · Cited by 262 · see citing works
Yeast Nuclear Extract Contains Two Major Forms of RNA Polymerase II Mediator Complexes
Liu, Y., Ranish, J. A., Aebersold, R. H., & Hahn, S. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m009586200 · Cited by 255 · see citing works
Nutritional skin care: health effects of micronutrients and fatty acids
Boelsma, E., Hendriks, H., & Roza, L. (2001) · American Journal of Clinical Nutrition · doi:10.1093/ajcn/73.5.853 · Cited by 243 · see citing works
Kang, S., Chung, J. H., Lee, J. H., Fisher, G. J., Wan, Y., Duell, E. A., & Voorhees, J. J. (2003) · Journal of Investigative Dermatology · doi:10.1046/j.1523-1747.2003.12122.x · Cited by 239 · see citing works
Self-citationBalbı́n, M., Fueyo, A., Knäuper, V., López, J. M., Álvarez, J., Sánchez, L. M., Quesada, V., Bordallo, J., Murphy, G., & López-Otı́n, C. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m007674200 · Cited by 239 · see citing works
Gum, R. J., Wang, H., Lengyel, E. R., et al. (1997) · Oncogene · doi:10.1038/sj.onc.1200973 · Cited by 236 · see citing works
Phillips, C. L., Combs, S. B., & Pinnell, S. R. (1994) · Journal of Investigative Dermatology · doi:10.1111/1523-1747.ep12393187 · Cited by 202 · see citing works
Saliou, C., Rimbach, G. H., Moini, H., McLaughlin, L., Hosseini, S., Lee, J., WATSON, R. R., & Packer, L. (2001) · Free Radical Biology and Medicine · doi:10.1016/s0891-5849(00)00445-7 · Cited by 147 · see citing works
Self-citationAfaq, F., Ahmad, N., & Mukhtar, H. (2003) · Oncogene · doi:10.1038/sj.onc.1207035 · Cited by 133 · see citing works
Holman, C., Armstrong, B. K., EVANS, P. R., LUMSDEN, G. J., DALLIMORE, K. J., Meehan, C., BEAGLEY, J., & GIBSON, I. M. (1984) · British Journal of Dermatology · doi:10.1111/j.1365-2133.1984.tb07458.x · Cited by 110 · see citing works
Raf and Mitogen-activated Protein Kinase Regulate Stellate Cell Collagen Gene Expression
Davis, B. H., Chen, A., & Beno, D. W. A. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.19.11039 · Cited by 90 · see citing works
Prevention of the photodamage in the hairless mouse dorsal skin by kojic acid as an iron chelator
Mitani, H., Koshiishi, I., Sumita, T., & Imanari, T. (2001) · European Journal of Pharmacology · doi:10.1016/s0014-2999(00)00873-6 · Cited by 81 · see citing works
Kambayashi, H., Yamashita, M., Odake, Y., et al. (2001) · Journal of Dermatological Science · doi:10.1016/s0923-1811(01)00113-x · Cited by 73 · see citing works
Mariani, T. J., Sandefur, S. L., Roby, J. D., & Pierce, R. A. (1998) · Molecular Biology of the Cell · doi:10.1091/mbc.9.6.1411 · Cited by 49 · see citing works
Photoprotective Effect of Black Tea Extracts Against UVB-induced Phototoxicity in Skin
Zhao, J., Jin, X., Yaping, E., Zheng, Z. S., Zhang, Y. J., Athar, M., DeLeo, V. A., Mukhtar, H., Bickers, D. R., & Wang, Z. Y. (1999) · Photochemistry and Photobiology · doi:10.1111/j.1751-1097.1999.tb08263.x · Cited by 48 · see citing works
Influence of oral antioxidants on ultraviolet radiation‐induced skin damage in humans
Swindells, K. J., & Rhodes, L. E. (2004) · Photodermatology Photoimmunology & Photomedicine · doi:10.1111/j.1600-0781.2004.00121.x · Cited by 44 · see citing works
Zaugg, J., Potterat, O., Plescher, A., Honermeier, B., & Hamburger, M. O. (2006) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf0611466 · Cited by 39 · see citing works
Antioxidant potential of evening primrose oil administration in hyperlipemic rabbits
Cruz, J. P. D. L., Quintero, L., Gálvez, J., et al. (1999) · Life Sciences · doi:10.1016/s0024-3205(99)00275-1 · Cited by 27 · see citing works
Määttå, A., Glumoff, V., Paakkonen, P., Liska, D. J., Penttinen, R. P. K., & Elima, K. (1993) · Biochemical Journal · doi:10.1042/bj2940365 · Cited by 27 · see citing works
Natural remedies for scleroderma.
Gaby, A. R. (2006) · PubMed · Cited by 22 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Bioactive Compounds for Skin Health: A Review
Michalak, M., Pierzak, M., Kręcisz, B., & Suliga, E. (2021) · Nutrients · doi:10.3390/nu13010203 · Cited by 345 · see citing works
Sun, Z., Park, S. Y., Hwang, E., Park, B., Seo, S. A., Cho, J.-G., Zhang, M., & Yi, T.-H. (2016) · Phytomedicine · doi:10.1016/j.phymed.2016.06.008 · Cited by 57 · see citing works
Self-citationOral supplementation with cocoa extract reduces UVB-Induced wrinkles in hairless mouse skin
Kim, J.-E., Song, D., Kim, J., Choi, J., Kim, J. R., Yoon, H.-S., Bae, J.-S., Han, M., Lee, S., Hong, J. S., et al. (2016) · Journal of Investigative Dermatology · doi:10.1016/j.jid.2015.11.032 · Cited by 37 · see citing works
Self-citation
- Authors
- Cho, H., Lee, M., Lee, J. W., No, K., Park, S., Lee, H., Kang, S., Cho, W., Park, H. J., Oh, K. W., & Hong, J. T.
- Affiliations
- Chungbuk National University (KR)
- LG (South Korea) (KR)
- Jeonju University (KR)
- Corresponding author
- Hong, Jin Tae
- Venue
- Photodermatology, Photoimmunology & Photomedicine
- Publisher
- Wiley
- Published
- 2007-08-30
- Volume
- 23
- Issue
- 5
- Pages
- 155-162
- ISSN
- 0905-4383 (ISSN-L)
- 1600-0781
- OpenAlex
- W2040652978
- Cited by
- 105
- References
- 30
- Citations by year
- 2025: 2 · 2024: 7 · 2023: 5 · 2022: 7 · 2021: 5 · 2020: 1 · 2019: 4 · 2018: 1 · 2017: 4 · 2016: 9 · 2015: 5 · 2014: 8 · 2013: 12 · 2012: 3
- FWCI
- 9.48
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Photodermatology Photoimmunology & Photomedicine (publishedVersion)
- PubMed (publishedVersion)
- Funders
- AGE-WELL
- Topics
- Health Sciences › Medicine › Dermatology › Skin Protection and Aging
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › melanin and skin pigmentation
- Health Sciences › Medicine › Dermatology › Dermatologic Treatments and Research
- Keywords
- vitamin C, vitamin E, Pycnogenol, evening primrose oil, antioxidant combination, UV-B radiation, photoaging, wrinkle formation, matrix metalloproteinases, collagen synthesis, type I procollagen, TGF-beta2, mitogen-activated protein kinase, AP-1 transcription factor, epidermal hyperplasia, hairless mouse skin, photoprotection
- MeSH terms
- Administration, Oral, Animals, Antioxidants, Ascorbic Acid, Flavonoids, Linoleic Acids, Mice, Hairless, Plant Extracts, Plant Oils, Ultraviolet Rays, Vitamin E, Skin Aging, gamma-Linolenic Acid, Oenothera biennis, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
