Contributions of Kv3 Channels to Neuronal Excitability
Abstract
Four mammalian Kv3 genes have been identified, each of which generates, by alternative splicing, multiple protein products differing in their C-terminal sequence. Products of the Kv3.1 and Kv3.2 genes express similar delayed-rectifier type currents in heterologous expression systems, while Kv3.3 and Kv3.4 proteins express A-type currents. All Kv3 currents activate relatively fast at voltages more positive than -10 mV, and deactivate very fast. The distribution of Kv3 mRNAs in the rodent CNS was studied by in situ hybridization, and the localization of Kv3.1 and Kv3.2 proteins has been studied by immunohistochemistry. Most Kv3.2 mRNAs (approximately 90%) are present in thalamic-relay neurons throughout the dorsal thalamus. The protein is expressed mainly in the axons and terminals of these neurons. Kv3.2 channels are thought to be important for thalamocortical signal transmission. Kv3.1 and Kv3.2 proteins are coexpressed in some neuronal populations such as in fast-spiking interneurons of the cortex and hippocampus, and neurons in the globus pallidus. Coprecipitation studies suggest that in these cells the two types of protein form heteromeric channels. Kv3 proteins appear to mediate, in native neurons, similar currents to those seen in heterologous expression systems. The activation voltage and fast deactivation rates are believed to allow these channels to help repolarize action potentials fast without affecting the threshold for action potential generation. The fast deactivating current generates a quickly recovering after hyperpolarization, thus maximizing the rate of recovery of Na+ channel inactivation without contributing to an increase in the duration of the refractory period. These properties are believed to contribute to the ability of neurons to fire at high frequencies and to help regulate the fidelity of synaptic transmission. Experimental evidence has now become available showing that Kv3.1-Kv3.2 channels play critical roles in the generation of fast-spiking properties in cortical GABAergic interneurons.
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Kv3.2 subunits, but not Kv3.1 subunits, possess a consensus sequence for protein kinase A (PKA) phosphorylation.
“Kv3.2 subunits, but not Kv3.1 subunits, possess a consensus sequence for protein kinase A (PKA) phosphorylation (803, 965).”
From Hippocampal GABAergic Inhibitory Interneurons (2017) · cites this paper as [965]
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- Authors
- RUDY, B., CHOW, A., LAU, D., AMARILLO, Y., OZAITA, A., SAGANICH, M., MORENO, H., NADAL, M. S., HERNANDEZ‐PINEDA, R., HERNANDEZ‐CRUZ, A., ERISIR, A., LEONARD, C., & DE Miera, E. V.
- Affiliations
- New York University (US)
- New York Medical College (US)
- Corresponding author
- RUDY, BERNARDO
- Venue
- Annals of the New York Academy of Sciences
- Publisher
- Wiley
- Published
- 1999-04
- Volume
- 868
- Issue
- 1
- Pages
- 304-343
- ISSN
- 0077-8923 (ISSN-L)
- 1749-6632
- OpenAlex
- W2126468386
- Cited by
- 305
- References
- 98
- Citations by year
- 2026: 5 · 2025: 7 · 2024: 11 · 2023: 6 · 2022: 8 · 2021: 6 · 2020: 11 · 2019: 4 · 2018: 10 · 2017: 12 · 2016: 7 · 2015: 12 · 2014: 3 · 2013: 6 · 2012: 5
- FWCI
- 5.97
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Annals of the New York Academy of Sciences (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (1r01ns035215-01a1, 5r01ns030989-22)
- National Institute of Neurological Disorders and Stroke (NS-30989, NS27881, NS-35215)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiac electrophysiology and arrhythmias
- Keywords
- Kv3 potassium channels, neuronal excitability, Kv3.1, Kv3.4, A-type potassium current, delayed rectifier potassium current, alternative splicing, thalamocortical neurons, thalamocortical transmission, fast-spiking interneurons, globus pallidus, heteromeric channels, action potential repolarization, refractory period, afterhyperpolarization
- MeSH terms
- Amino Acid Sequence, Animals, Brain Chemistry, Cell Line, Gene Expression Regulation, Molecular Sequence Data, Neurons, Protein Kinases, RNA, Potassium Channels, Sequence Alignment, Alternative Splicing, In Situ Hybridization, Evolution, Molecular, Potassium Channels, Voltage-Gated, Mice, Rats, Shaw Potassium Channels
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