Cited by 97Neuroscience and Neuropharmacology ResearchIon channel regulation and functionNeurotransmitter Receptor Influence on Behavior

GABA‐Ergic interneurons of the striatum express the shaw‐like potassium channel KvS3.1

Lenz, S., Perney, T. M., Qin, Y., Robbins, E., Chesselet, M.‐F.·1994·Synapse

Abstract

In addition to numerous GABA-ergic efferent neurons, the striatum contains a subpopulation of fast-firing GABA-ergic interneurons characterized by the presence of immunoreactivity for the calcium-binding protein, parvalbumin. Double-label in situ hybridization with digoxigenin- and radiolabelled cRNA probes was performed on striatal sections of adult rats to identify mRNAs expressed by striatal GABA-ergic interneurons. In the dorsolateral striatum, only parvalbumin mRNA-positive neurons expressed the mRNA encoding the potassium channel Kv3.1, a member of the Shaw family of potassium channels with rapid activation and inactivation kinetics, usually found in fast-firing neurons such as the basket cells of the hippocampus. Colocalization of the parvalbumin and Kv3.1 proteins was confirmed by double-label immunohistochemistry. Parvalbumin mRNA-positive neurons expressed very high levels of the mRNA encoding glutamic acid decarboxylase (Mr 67,000: GAD67) in the dorsolateral striatum. A smaller proportion of double-labelled neurons was found in the ventrolateral striatum. A small number of densely labelled neurons for GAD67 mRNA also expressed the mRNA encoding the dopamine D2 receptor, but none expressed detectable levels of the dopamine D1 receptor mRNA. This indicates major differences in the expression of dopamine receptor mRNA in a majority of GABA-ergic interneurons vs. GABA-ergic efferent neurons of the striatum. The results suggest that distinct molecular characteristics are associated with the distinct electrophysiological properties of striatal GABA-ergic neurons.

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Crossref · 10.1002/syn.890180108

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Authors
Lenz, S., Perney, T. M., Qin, Y., Robbins, E., & Chesselet, M. ‐.
Affiliations
  • University of Pennsylvania (US)
  • Yale University (US)
Corresponding author
Chesselet, M.‐F.
Venue
Synapse
Publisher
Wiley
Published
1994-09
Volume
18
Issue
1
Pages
55-66
ISSN
  • 0887-4476 (ISSN-L)
  • 1098-2396
OpenAlex
W1991704903
Cited by
97
References
47
Citations by year
2025: 1 · 2024: 1 · 2023: 1 · 2022: 2 · 2021: 1 · 2020: 2 · 2019: 1 · 2018: 2 · 2017: 4 · 2016: 2 · 2015: 3 · 2014: 2 · 2013: 1 · 2012: 1
FWCI
1.03
Type
journal article
Language
en
Open access
closed
Locations
  • Synapse (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institute of Mental Health (MH-44894)
  • National Institute on Deafness and Other Communication Disorders (DC01919, R01DC001919)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurotransmitter Receptor Influence on Behavior
Keywords
GABAergic interneurons, striatum, Kv3.1 potassium channel, parvalbumin, GAD67, dopamine D2 receptor, dopamine D1 receptor, dorsolateral striatum, ventrolateral striatum, basket cells, gene expression
MeSH terms
Animals, Digoxigenin, Electrophysiology, gamma-Aminobutyric Acid, Glutamate Decarboxylase, Immunohistochemistry, Interneurons, Male, Parvalbumins, RNA, Messenger, Potassium Channels, RNA Probes, Neostriatum, Rats, Sprague-Dawley, In Situ Hybridization, Receptors, Dopamine D1, Receptors, Dopamine D2, Rats

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