Isolating and evaluating lactic acid bacteria strains for effectiveness of Leymus chinensis silage fermentation
Abstract
Five LAB strains were evaluated using the acid production ability test, morphological observation, Gram staining, physiological, biochemical and acid tolerance tests. All five strains (LP1, LP2, LP3, LC1 and LC2) grew at pH 4·0, and LP1 grew at 15°C. Strains LP1, LP2 and LP3 were identified as Lactobacillus plantarum, whereas LC1 and LC2 were classified as Lactobacillus casei by sequencing 16S rDNA. The five isolated strains and two commercial inoculants (PS and CL) were added to native grass and Leymus chinensis (Trin.) Tzvel. for ensiling. All five isolated strains decreased the pH and ammonia nitrogen content, increased the lactic acid content and LP1, LP2 and LP3 increased the acetic content and lactic/acetic acid ratio of L. chinensis silage significantly. The five isolated strains and two commercial inoculants decreased the butyric acid content of the native grass silage. LP2 treatment had lower butyric acid content and ammonia nitrogen content than the other treatments. The five isolated strains improved the quality of L. chinensis silage. The five isolated strains and the two commercial inoculants were not effective in improving the fermentation quality of the native grass silage, but LP2 performed better comparatively. Significance and impact of the study: Leymus chinensis is an important grass in China and Russia, being the primary grass of the short grassland 'steppe' regions of central Asia. However, it has been difficult to make high-quality silage of this species because of low concentration of water-soluble carbohydrates (WSC). Isolating and evaluating lactic acid bacteria strains will be helpful for improving the silage quality of this extensively grown species.
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The addition of LAB inoculants at ensiling is intended to ensure rapid and vigorous fermentation.
“the addition of LAB inoculants at ensiling is intended to ensure rapid and vigorous fermentation [45].”
Cites
Thompson, J. D., Higgins, D. G., & Gibson, T. J. (1994) · Nucleic Acids Research · doi:10.1093/nar/22.22.4673 · Cited by 64,617 · see citing works
The neighbor-joining method: a new method for reconstructing phylogenetic trees.
Saitou, N., & Nei, M. (1987) · Molecular Biology and Evolution · doi:10.1093/oxfordjournals.molbev.a040454 · Cited by 59,962 · see citing works
Official Methods of Analysis of
Helrick, K. (1980) · Analytical Chemistry · doi:10.1021/ac50052a726 · Cited by 59,351 · see citing works
Van Soest, P. J., Robertson, J. B., & Lewis, B. A. (1991) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(91)78551-2 · Cited by 28,658 · see citing works
Broderick, G. A., & Kang, J. H. (1980) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(80)82888-8 · Cited by 2,523 · see citing works
Clarridge, J. E. (2004) · Clinical Microbiology Reviews · doi:10.1128/cmr.17.4.840-862.2004 · Cited by 2,198 · see citing works
Complete genome sequence of <i>Lactobacillus plantarum</i> WCFS1
Kleerebezem, M., Boekhorst, J., van Kranenburg, R., Molenaar, D., Kuipers, O. P., Leer, R., Tarchini, R., Peters, S. A., Sandbrink, H. M., Fiers, M. W. E. J., et al. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0337704100 · Cited by 1,472 · see citing works
(2003) · Agronomy Monographs · doi:10.2134/agronmonogr42 · Cited by 881 · see citing works
The buffering constituents of herbage and of silage
PLAYNE, M. J., & McDonald, P. (1966) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.2740170609 · Cited by 744 · see citing works
Acetic acid increases stability of silage under aerobic conditions
Danner, H., Holzer, M., Mayrhuber, E., & Braun, R. (2003) · Applied and Environmental Microbiology · doi:10.1128/aem.69.1.562-567.2003 · Cited by 515 · see citing works
Cai, Y., Benno, Y., OGAWA, M., Ohmomo, S., KUMAI, S., & Nakase, T. (1998) · Applied and Environmental Microbiology · doi:10.1128/aem.64.8.2982-2987.1998 · Cited by 393 · see citing works
On the stochastic model for estimation of mutational distance between homologous proteins
Kimura, M., & Ohta, T. (1972) · Journal of Molecular Evolution · doi:10.1007/bf01653945 · Cited by 391 · see citing works
Cai, Y., Benno, Y., Ogawa, M., & Kumai, S. (1999) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(99)75263-x · Cited by 328 · see citing works
A method for the extraction of plant samples and the determination of total soluble carbohydrates
Murphy, R. P. (1958) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.2740091104 · Cited by 267 · see citing works
Hu, W., Schmidt, R. J., McDonell, E. E., Klingerman, C. M., & Kung, L. (2009) · Journal of Dairy Science · doi:10.3168/jds.2008-1788 · Cited by 211 · see citing works
Cai, Y., KUMAI, S., OGAWA, M., Benno, Y., & Nakase, T. (1999) · Applied and Environmental Microbiology · doi:10.1128/aem.65.7.2901-2906.1999 · Cited by 179 · see citing works
Lin, C., Bolsen, K. K., Brent, B. E., & Fung, D. Y. C. (1992) · Journal of Applied Bacteriology · doi:10.1111/j.1365-2672.1992.tb04992.x · Cited by 161 · see citing works
Contreras-Govea, F. E., Muck, R. E., Mertens, D. R., & Weimer, P. J. (2011) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2010.09.015 · Cited by 161 · see citing works
Proteolysis During Ensilage of Forages Varying in Soluble Sugar Content
Davies, D. R., Merry, R. J., Williams, A. P., Bakewell, E. L., Leemans, D. K., & Tweed, J. K. S. (1998) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(98)75596-1 · Cited by 151 · see citing works
Inoculant effects on alfalfa silage: Fermentation products and nutritive value
Filya, I., Muck, R. E., & Contreras-Govea, F. E. (2007) · Journal of Dairy Science · doi:10.3168/jds.2006-877 · Cited by 145 · see citing works
Tabacco, E., Piano, S., Revello-Chion, A., & Borreani, G. (2011) · Journal of Dairy Science · doi:10.3168/jds.2011-4286 · Cited by 132 · see citing works
Arriola, K. G., Kim, S. C., & Adesogan, A. T. (2011) · Journal of Dairy Science · doi:10.3168/jds.2010-3807 · Cited by 129 · see citing works
Heinritz, S. N., Martens, S. D., Avila, P., & Hoedtke, S. (2012) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2012.03.017 · Cited by 125 · see citing works
The use of Lactobacillus species as starter cultures for enhancing the quality of sugar cane silage
Ávila, C. L. S., Carvalho, B. F., Pinto, J. C., Duarte, W. F., & Schwan, R. F. (2014) · Journal of Dairy Science · doi:10.3168/jds.2013-6987 · Cited by 125 · see citing works
Parvin, S., Wang, C., Li, Y., & Nishino, N. (2010) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2010.07.010 · Cited by 101 · see citing works
Escalante, A., Wacher, C., & Farrés, A. (2001) · International Journal of Food Microbiology · doi:10.1016/s0168-1605(00)00428-1 · Cited by 87 · see citing works
Peng, X., Zhang, L., Zhang, L., et al. (2012) · Plant Cell Tissue and Organ Culture (PCTOC) · doi:10.1007/s11240-012-0264-0 · Cited by 62 · see citing works
Chen, M., Liu, Q. H., Xin, G. R., & Zhang, J. G. (2012) · Letters in Applied Microbiology · doi:10.1111/lam.12018 · Cited by 61 · see citing works
Tohno, M., Kobayashi, H., Nomura, M., Kitahara, M., Ohkuma, M., Uegaki, R., & Cai, Y. (2011) · Animal Science Journal · doi:10.1111/j.1740-0929.2011.00923.x · Cited by 58 · see citing works
OHYAMA, Y., MORICHI, T., & Masaki, S. (1975) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.2740260717 · Cited by 54 · see citing works
Guo, X., Undersander, D. J., & Combs, D. K. (2013) · Journal of Dairy Science · doi:10.3168/jds.2045-5786 · Cited by 53 · see citing works
Owens, V. N., Albrecht, K. A., & Muck, R. E. (1999) · Canadian Journal of Plant Science · doi:10.4141/p98-034 · Cited by 50 · see citing works
Yan, R., Chen, S., Zhang, X., et al. (2011) · Journal of Dairy Science · doi:10.3168/jds.2010-3536 · Cited by 35 · see citing works
Shu, Q., Liu, G. S., Xu, S., et al. (2005) · Plant Cell Reports · doi:10.1007/s00299-004-0908-6 · Cited by 29 · see citing works
Woolford, M. K., & Wilkie, A. C. (1984) · The Journal of Agricultural Science · doi:10.1017/s0021859600041502 · Cited by 20 · see citing works
Kim, H., Joung, Y., & Joh, K. (2013) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/ijs.0.055434-0 · Cited by 17 · see citing works
Zhan, S. (2007) · Acta Agrestia Sinica · Cited by 5 · see citing works
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Alhaag, H., Yuan, X., Mala, A., Bai, J., & Shao, T. (2019) · Applied Sciences · doi:10.3390/app9061247 · Cited by 55 · see citing works
Zhang, Q., Yu, Z., Yang, H., & Na, R. S. (2016) · Grass and Forage Science · doi:10.1111/gfs.12210 · Cited by 52 · see citing works
Self-citation
- Authors
- Zhang, Q., Li, X. J., Zhao, M. M., & Yu, Z.
- Affiliations
- China Agricultural University (CN)
- Corresponding author
- Yu, Z.
- Venue
- Letters in Applied Microbiology
- Publisher
- Oxford University Press (OUP)
- Published
- 2014-07-02
- Volume
- 59
- Issue
- 4
- Pages
- 391-397
- ISSN
- 0266-8254 (ISSN-L)
- OpenAlex
- W2071474178
- Cited by
- 36
- References
- 37
- Citations by year
- 2025: 2 · 2024: 3 · 2023: 1 · 2022: 2 · 2021: 9 · 2020: 4 · 2019: 2 · 2018: 3 · 2017: 4 · 2016: 2 · 2015: 4
- FWCI
- 6.65
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Letters in Applied Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Special Fund of National Public Welfare Industry (Agriculture) R & D Program (201303061)
- National Key Technology R&D Programme (2011BAD17B02)
- China Forage and Grass Research System (CARS-35)
- Beijing Key Laboratory of Grassland Science
- Topics
- Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Ruminant Nutrition and Digestive Physiology
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
- Keywords
- Leymus chinensis, silage fermentation, lactic acid bacteria, Lactobacillus plantarum, Lactobacillus casei, silage inoculants, silage quality, ammonia nitrogen, butyrate, lactic acid, acetic acid, water-soluble carbohydrates
- MeSH terms
- Fermentation, Food Handling, Poaceae, Lacticaseibacillus casei, Phylogeny, Silage, Lactiplantibacillus plantarum
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