Quantitative proteomics of a B12‐dependent alga grown in coculture with bacteria reveals metabolic tradeoffs required for mutualism
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Summary The unicellular green alga Lobomonas rostrata requires an external supply of vitamin B12 (cobalamin) for growth, which it can obtain in stable laboratory cultures from the soil bacterium Mesorhizobium loti in exchange for photosynthate. We investigated changes in protein expression in the alga that allow it to engage in this mutualism. We used quantitative isobaric tagging (iTRAQ) proteomics to determine the L. rostrata proteome grown axenically with B12 supplementation or in coculture with M. loti. Data are available via ProteomeXchange (PXD005046). Using the related Chlamydomonas reinhardtii as a reference genome, 588 algal proteins could be identified. Enzymes of amino acid biosynthesis were higher in coculture than in axenic culture, and this was reflected in increased amounts of total cellular protein and several free amino acids. A number of heat shock proteins were also elevated. Conversely, photosynthetic proteins and those of chloroplast protein synthesis were significantly lower in L. rostrata cells in coculture. These observations were confirmed by measurement of electron transfer rates in cells grown under the two conditions. The results indicate that, despite the stability of the mutualism, L. rostrata experiences stress in coculture with M. loti, and must adjust its metabolism accordingly.
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M. loti supplies vitamin B12 to L. rostrata, and in return receives fixed carbon from the microalga.
“A model interaction for the exchange of vitamin B12 is the one created between the soil rhizobiaceae Mesorhizobium loti and the freshwater green microalga Lobomonas rostrata, where M. loti supplies vitamin B12, and in return, the bacterium receives fixed carbon from the microalga [45,46,47].”
From Toward the Enhancement of Microalgal Metabolite Production through Microalgae–Bacteria Consortia (2021) · cites this paper as [47]
The co-culture induces some stress in L. rostrata, and it must adjust its metabolism accordingly.
“Despite the stability of this synthetic mutualism, the responses of L. rostrata show that the co-culture induces some stress in the microalga, and it must adjust its metabolism accordingly [47].”
From Toward the Enhancement of Microalgal Metabolite Production through Microalgae–Bacteria Consortia (2021) · cites this paper as [47]
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Toward the Enhancement of Microalgal Metabolite Production through Microalgae–Bacteria Consortia
González-González, L. M., & de-Bashan, L. E. (2021) · Biology · doi:10.3390/biology10040282 · Cited by 133 · see citing works
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- Authors
- Helliwell, K. E., Pandhal, J., Cooper, M. B., Longworth, J., Kudahl, U. J., Russo, D. A., Tomsett, E. V., Bunbury, F., Salmon, D. L., Smirnoff, N., Wright, P. C., & Smith, A. G.
- Affiliations
- University of Cambridge (GB)
- University of Sheffield (GB)
- University of Exeter (GB)
- Corresponding author
- Pandhal, Jagroop
- Smith, Alison G.
- ORCID
- Venue
- New Phytologist
- Publisher
- Wiley
- Published
- 2017-10-16
- Volume
- 217
- Issue
- 2
- Pages
- 599-612
- ISSN
- 0028-646X (ISSN-L)
- 1469-8137
- PMC
- PMC5765456
- OpenAlex
- W2766386390
- Cited by
- 48
- References
- 65
- Citations by year
- 2026: 4 · 2025: 7 · 2024: 9 · 2023: 1 · 2022: 7 · 2021: 5 · 2020: 5 · 2019: 6 · 2018: 4
- FWCI
- 1.10
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- New Phytologist (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- White Rose Research Online (University of Leeds, The University of Sheffield, University of York) (acceptedVersion)
- Marine Biological Association (acceptedVersion)
- Apollo (University of Cambridge) (submittedVersion, cc-by)
- Open Repository and Bibliography (University of Luxembourg) (submittedVersion)
- Apollo (cc-by)
- Funders
- Engineering and Physical Sciences Research Council (EP/E036252/1)
- Biotechnology and Biological Sciences Research Council (BB/I013164/1)
- Natural Environment Research Council (NE/J024767/1)
- EU Framework Programme 7 (Marie Curie ITN Photo.Comm no. 317184 (for UJK))
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protist diversity and phylogeny
- Keywords
- Mesorhizobium loti, vitamin B12, photosynthetic electron transport, amino acid biosynthesis, heat shock proteins, chloroplast protein synthesis, co-culture
- MeSH terms
- Chlorophyta, Amino Acids, Electron Transport, Photosynthesis, RNA, Messenger, Symbiosis, Vitamin B 12, Gene Expression Regulation, Plant, Coculture Techniques, Computational Biology, Algal Proteins, Proteomics, Mesorhizobium
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