Proteome response of Phaeodactylum tricornutum , during lipid accumulation induced by nitrogen depletion
Open accessAbstract
Nitrogen stress is a common strategy employed to stimulate lipid accumulation in microalgae, a biofuel feedstock of topical interest. Although widely investigated, the underlying mechanism of this strategy is still poorly understood. We examined the proteome response of lipid accumulation in the model diatom, Phaeodactylum tricornutum (CCAP 1055/1), at an earlier stage of exposure to selective nitrogen exclusion than previously investigated, and at a time point when changes would reflect lipid accumulation more than carbohydrate accumulation. In total 1043 proteins were confidently identified (≥ 2 unique peptides) with 645 significant ( p < 0.05) changes observed, in the LC-MS/MS based iTRAQ investigation. Analysis of significant changes in KEGG pathways and individual proteins showed that under nitrogen starvation P. tricornutum reorganizes its proteome in favour of nitrogen scavenging and reduced lipid degradation whilst rearranging the central energy metabolism that deprioritizes photosynthetic pathways. By doing this, this species appears to increase nitrogen availability inside the cell and limit its use to the pathways where it is needed most. Compared to previously published proteomic analysis of nitrogen starvation in Chlamydomonas reinhardtii , central energy metabolism and photosynthesis appear to be affected more in the diatom, whilst the green algae appears to invest its energy in reorganizing respiration and the cellular organization pathways.
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Neutral lipid levels are significantly higher than carbohydrates under nitrogen depletion.
“In that case, neutral lipid levels are significantly higher than carbohydrates [122].”
From Microalgae biofuels: illuminating the path to a sustainable future amidst challenges and opportunities (2024) · cites this paper as [122]
The Results and discussion section (‘Biochemical characterization under nitrogen stress’) says neutral lipid levels were significantly higher than carbohydrates **in relative terms**—as biomass-normalized responses to nitrogen depletion compared with nitrogen-replete controls. The citing sentence omits that qualification and could be read as claiming higher absolute lipid levels, which this passage does not establish. (LLM, conf=0.86)
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- Authors
- Longworth, J., Wu, D., Huete-Ortega, M., Wright, P. C., & Vaidyanathan, S.
- Affiliations
- University of Sheffield (GB)
- Corresponding author
- Vaidyanathan, Seetharaman
- Venue
- Algal Research
- Publisher
- Elsevier BV
- Published
- 2016-09
- Volume
- 18
- Pages
- 213-224
- ISSN
- 2211-9264 (ISSN-L)
- PMC
- PMC5070409
- OpenAlex
- W2474275281
- Cited by
- 127
- References
- 71
- Citations by year
- 2026: 7 · 2025: 4 · 2024: 18 · 2023: 11 · 2022: 18 · 2021: 13 · 2020: 14 · 2019: 19 · 2018: 12 · 2017: 11
- FWCI
- 3.80
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- Algal Research (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- White Rose Research Online (University of Leeds, The University of Sheffield, University of York) (acceptedVersion)
- Open Repository and Bibliography (University of Luxembourg) (submittedVersion)
- Funders
- EPSRC (EP/E036252/1)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- Phaeodactylum tricornutum, nitrogen starvation, lipid accumulation, nitrogen scavenging, lipid degradation, photosynthesis, biofuel feedstock
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