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Cited by 27

The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.

Improved recovery of ancient DNA from subfossil wood – application to the world's oldest Late Glacial pine forest

Lendvay, Bertalan, Hartmann, Martin, Brodbeck, Sabine, Nievergelt, Daniel, Reinig, Frederick, Zoller, Stefan, Parducci, Laura, Gugerli, Felix, Büntgen, Ulf, Sperisen, Christoph·2018·New Phytologist
DOI 10.1111/nph.14935
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Abstract

Summary: Ancient DNA from historical and subfossil wood has a great potential to provide new insights into the history of tree populations. However, its extraction and analysis have not become routine, mainly because contamination of the wood with modern plant material can complicate the verification of genetic information. Here, we used sapwood tissue from 22 subfossil pines that were growing c. 13 000 yr bp in Zurich, Switzerland. We developed and evaluated protocols to eliminate surface contamination, and we tested ancient DNA authenticity based on plastid DNA metabarcoding and the assessment of post‐mortem DNA damage. A novel approach using laser irradiation coupled with bleaching and surface removal was most efficient in eliminating contaminating DNA. DNA metabarcoding confirmed which ancient DNA samples repeatedly amplified pine DNA and were free of exogenous plant taxa. Pine DNA sequences of these samples showed a high degree of cytosine to thymine mismatches, typical of post‐mortem damage. Stringent decontamination of wood surfaces combined with DNA metabarcoding and assessment of post‐mortem DNA damage allowed us to authenticate ancient DNA retrieved from the oldest Late Glacial pine forest. These techniques can be applied to any subfossil wood and are likely to improve the accessibility of relict wood for genome‐scale ancient DNA studies.

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  • New sources of archaeological material have broadened the range of ancient DNA applications, including dental calculus, wood, mollusk shells, sediments, parchments, and textiles.

    “These include dental calculus 26,44 , wood 45,46 , mollusk shells 47 , and sediments 48,49 , as well as biocultural archives such as parchments 50 and textiles 51 .”

    From Ancient DNA analysis (2021) · cites this paper as [46]

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Cited by

Sorted by citations

  • Ancient DNA analysis

    Orlando, L., Allaby, R., Skoglund, P., Der Sarkissian, C., Stockhammer, P. W., Ávila-Arcos, M. C., Fu, Q., Krause, J., Willerslev, E., Stone, A. C., et al. (2021) · Nature Reviews Methods Primers · doi:10.1038/s43586-020-00011-0 · Cited by 411

  • Dietary DNA Metabarcoding From Animal Fecal Samples

    McConnell, R. D., Jarrett, C., Ferreira, D. F., Powell, L. L., Quiñones, A. L. S., Dominoni, D. M., & Welch, A. J. (2026) · Current Protocols · doi:10.1002/cpz1.70226 · Cited by 2

    “AmpliTaq Gold has been used often in the field of ancient DNA for work with degraded DNA (Grealy et al., 2015; Lendvay et al., 2018; D. C. Mur-ray et al., 2012).”

Metadata

Authors
Lendvay, B., Hartmann, M., Brodbeck, S., Nievergelt, D., Reinig, F., Zoller, S., Parducci, L., Gugerli, F., Büntgen, U., & Sperisen, C.
Affiliations
  • Swiss Federal Institute for Forest Snow and Landscape Research WSL Zürcherstrasse 111 CH‐8903 Birmensdorf Switzerland
  • Genetic Diversity Centre ETH Zurich Universitätstrasse 16 CH‐8092 Zurich Switzerland
  • Department of Ecology and Genetics Evolutionary Biology Centre Uppsala University Norbyvägen 18D 75236 Uppsala Sweden
Venue
New Phytologist
Publisher
Wiley
Published
2017-12-15
Volume
217
Issue
4
Pages
1737-1748
ISSN
  • 0028-646X
  • 1469-8137
DOI
10.1111/nph.14935
Cited by
27
References
7
Language
en
Licence
Funders
  • Ministerul Educatiei Nationale
  • Vetenskapsrådet (2013‐D0568401, 2013-D0568401)
  • Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (200021L_157187/1)
  • Youth and Sports of Czech Republic, National Sustainability Program I (NPU I) (LO1415)

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