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From the field to the laboratory: Controlling DNA contamination in human ancient DNA research in the high-throughput sequencing era

Llamas, Bastien, Valverde, Guido, Fehren-Schmitz, Lars, Weyrich, Laura S, Cooper, Alan, Haak, Wolfgang·2017·STAR: Science & Technology of Archaeological Research

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Crossref · 10.1080/20548923.2016.1258824

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  • mtDNA analyses rely upon amplification of ultratrace amounts of extant mtDNA using PCR, making the approach more sensitive and more susceptible to contamination.

    “mtDNA analyses rely upon amplification of ultratrace amounts of extant mtDNA using the PCR, thereby making such an approach not only more sensitive but also more susceptible to contamination (45).”

    From Pre-Clovis occupation of the Americas identified by human fecal biomarkers in coprolites from Paisley Caves, Oregon (2020) · cites this paper as [45]

    The brief technical overview says PCR was traditionally used to amplify specific DNA targets in ancient-DNA research, and the “Contaminating human DNA” section calls PCR amplification a potent source of contamination. The paper does not say that mtDNA analyses rely on PCR amplification of ultratrace amounts of extant mtDNA, or that this makes mtDNA analysis more sensitive and more susceptible to contamination. (LLM, conf=0.83)

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Metadata

Authors
Llamas, B., Valverde, G., Fehren-Schmitz, L., Weyrich, L. S., Cooper, A., & Haak, W.
Affiliations
  • Australian Centre for Ancient DNA, School of Biological Sciences, University of Adelaide, Adelaide, South Australia 5005, Australia
  • Department of Anthropology, University of California Santa Cruz, CA, 95064, USA
Venue
STAR: Science & Technology of Archaeological Research
Published
2016-11-30
Volume
3
Issue
1
Pages
1-14
ISSN
2054-8923
Cited by
160
References
28
Language
en

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