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From the field to the laboratory: Controlling DNA contamination in human ancient DNA research in the high-throughput sequencing era
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mtDNA analyses rely upon amplification of ultratrace amounts of extant mtDNA using PCR, making the approach more sensitive and more susceptible to contamination.
“mtDNA analyses rely upon amplification of ultratrace amounts of extant mtDNA using the PCR, thereby making such an approach not only more sensitive but also more susceptible to contamination (45).”
From Pre-Clovis occupation of the Americas identified by human fecal biomarkers in coprolites from Paisley Caves, Oregon (2020) · cites this paper as [45]
The brief technical overview says PCR was traditionally used to amplify specific DNA targets in ancient-DNA research, and the “Contaminating human DNA” section calls PCR amplification a potent source of contamination. The paper does not say that mtDNA analyses rely on PCR amplification of ultratrace amounts of extant mtDNA, or that this makes mtDNA analysis more sensitive and more susceptible to contamination. (LLM, conf=0.83)
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Citations
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Metadata
- Authors
- Llamas, B., Valverde, G., Fehren-Schmitz, L., Weyrich, L. S., Cooper, A., & Haak, W.
- Affiliations
- Australian Centre for Ancient DNA, School of Biological Sciences, University of Adelaide, Adelaide, South Australia 5005, Australia
- Department of Anthropology, University of California Santa Cruz, CA, 95064, USA
- Venue
- STAR: Science & Technology of Archaeological Research
- Publisher
- Informa UK Limited
- Published
- 2016-11-30
- Volume
- 3
- Issue
- 1
- Pages
- 1-14
- ISSN
- 2054-8923
- Cited by
- 160
- References
- 28
- Language
- en
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