A YadA‐like autotransporter, Hag1 in Veillonella atypica is a multivalent hemagglutinin involved in adherence to oral streptococci, Porphyromonas gingivalis, and human oral buccal cells

Zhou, P.; Liu, J.; Merritt, J.; Qi, F.

doi:10.1111/omi.12091

Cited by 52Oral microbiology and periodontitis researchBacterial biofilms and quorum sensingBiochemical and Structural Characterization

A YadA‐like autotransporter, Hag1 in Veillonella atypica is a multivalent hemagglutinin involved in adherence to oral streptococci, Porphyromonas gingivalis, and human oral buccal cells

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Zhou, P., Liu, J., Merritt, J., Qi, F.·2015·Molecular Oral Microbiology

DOI 10.1111/omi.12091PMC PMC4451445

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Abstract

Dental biofilm development is a sequential process, and adherence between microbes and the salivary pellicle (adhesion) as well as among different microbes (co-adhesion or coaggregation) plays a critical role in building a biofilm community. The Veillonella species are among the most predominant species in the oral cavity and coaggregate with many initial, early, middle, and late colonizers. Similar to oral fusobacteria, they are also considered bridging species in biofilm development. However, the mechanism of this ability has yet to be reported, due to the previous lack of a genetic transformation system in the entire genus. In this study, we used our recently discovered transformable Veillonella strain, Veillonella atypica OK5, to probe the mechanism of coaggregation between Veillonella species and other oral bacteria. By insertional inactivation of all eight putative hemagglutinin genes, we identified one gene, hag1, which is involved in V. atypica coaggregation with the initial colonizers Streptococcus gordonii, Streptococcus oralis and Streptococcus cristatus, and the periodontal pathogen Porphyromonas gingivalis. The hag1 mutant also abolished adherence to human buccal cells. Inhibition assays using various chemical or physiological treatments suggest different mechanisms being involved in coaggregation with different partners. The entire hag1 gene was sequenced and shown to be the largest known bacterial hemagglutinin gene.

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Veillonella atypica co-aggregates with a significant number of potential dental pathogens via its Hag1 TAA.
“The species Veillonella atypica co-aggregates with a significant number of potential dental pathogens via its Hag1 TAA [112].”
From Bacterial autoaggregation (2018) · cites this paper as [112]
The Results (Identification of putative hemagglutinins; page not provided) report that disrupting hag1 abolished coaggregation of V. atypica OK5 with three oral streptococci and P. gingivalis. The paper identifies P. gingivalis as a periodontal pathogen but describes the three streptococci as initial colonizers, so it does not establish coaggregation with a significant number of potential dental pathogens via Hag1. (LLM, conf=0.86)
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Authors: Zhou, P., Liu, J., Merritt, J., & Qi, F.
Affiliations: University of Oklahoma Health Sciences Center (US)
Corresponding author: Qi, F.
ORCID: Zhou, P.
Liu, J.
Merritt, J.
Venue: Molecular Oral Microbiology
Publisher: Wiley
Published: 2015-01-19
Volume: 30
Issue: 4
Pages: 269-279
ISSN: 2041-1006 (ISSN-L)
2041-1014
DOI: 10.1111/omi.12091
PMC: PMC4451445
OpenAlex: W2021787227
Cited by: 52
References: 41
Citations by year: 2026: 4 · 2025: 6 · 2024: 10 · 2023: 5 · 2022: 2 · 2021: 2 · 2020: 6 · 2019: 2 · 2018: 4 · 2017: 4 · 2016: 5 · 2015: 2
FWCI: 4.88
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Licence: onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations: Molecular Oral Microbiology (publishedVersion)
PubMed (publishedVersion)
Europe PMC (PubMed Central) (acceptedVersion)
PubMed Central (acceptedVersion)
Funders: National Institutes of Health (2R15DE019940)
Topics: Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Structural Characterization
Keywords: Veillonella atypica, co-aggregation, dental plaque, oral streptococci, Porphyromonas gingivalis, salivary pellicle, hemagglutinin gene, insertional inactivation, periodontal pathogens
MeSH terms: Type V Secretion Systems, Bacterial Adhesion, Bacterial Proteins, Genes, Bacterial, Hemagglutinins, Humans, Molecular Sequence Data, Mouth, Mouth Mucosa, Mutation, Streptococcus, Veillonella, Porphyromonas gingivalis, Sequence Analysis, DNA, Biofilms, Streptococcus oralis, Streptococcus gordonii, Microbial Interactions

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A YadA‐like autotransporter, Hag1 in Veillonella atypica is a multivalent hemagglutinin involved in adherence to oral streptococci, Porphyromonas gingivalis, and human oral buccal cells

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