Engineering herbicide resistance via prime editing in rice
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Although CRISPR-Cas9 has revolutionized our ability to generate site-specific double-strand breaks, precise editing of the genome remains challenging in most eukaryotes, including plants (Shan et al., 2013).In plants homology-directed repair is inefficient, limiting our ability to make precise edits of the
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The rice gene Oryza sativa acetolactate synthase (OsALS) was targeted to develop resistance to the herbicide bispyribac sodium.
“For instance, the rice gene Oryza sativa acetolactate synthase (OsALS) was targeted to develop resistance to the herbicide bispyribac sodium (Butt et al., 2020).”
From Genetic resources and precise gene editing for targeted improvement of barley abiotic stress tolerance (2023) · cites this paper as Butt et al., 2020
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Search-and-replace genome editing without double-strand breaks or donor DNA
Anzalone, A. V., Randolph, P. B., Davis, J. R., Sousa, A. A., Koblan, L. W., Levy, J. M., Chen, P. J., Wilson, C., Newby, G. A., Raguram, A., et al. (2019) · Nature · doi:10.1038/s41586-019-1711-4 · Cited by 4,767 · cited 5× · see citing works
“Researchers have developed several prime editing strategies: in PE1, wild type M-MLV RT fused to the C terminus of Cas9 (H840A) nickase; in PE2, Cas9 (H840A) with pentamutant M-MLV RT (D200N/ L603W/ T330P/ T306K/ W313F); in PE3, a PE2 prime editor with additional simple gRNA to simultaneously nick the non-edited stran…”
BackgroundMethodInfluentialTargeted genome modification of crop plants using a CRISPR-Cas system
Shan, Q., Wang, Y., Li, J., Zhang, Y., Chen, K., Liang, Z., Zhang, K., Liu, J., Xi, J., Qiu, J., et al. (2013) · Nature Biotechnology · doi:10.1038/nbt.2650 · Cited by 1,965 · cited 1× · see citing works
“Although CRISPR-Cas9 has revolutionized our ability to generate site-specific double-strand breaks, precise editing of the genome remains challenging in most eukaryotes, including plants (Shan et al., 2013).”
BackgroundBase editing: precision chemistry on the genome and transcriptome of living cells
Rees, H. A., & Liu, D. R. (2018) · Nature Reviews Genetics · doi:10.1038/s41576-018-0059-1 · Cited by 1,675 · cited 1× · see citing works
“Moreover, cytosine and adenine base editors have serious drawbacks including lower efficiency, unclean edited sequence and the possibility of off-target mutations at other loci (Rees and Liu, 2018).”
BackgroundBoosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system
Xie, K., Minkenberg, B., & Yang, Y. (2015) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1420294112 · Cited by 1,532 · cited 1× · see citing works
“We designed the sgRNA to target OsALS at a distance of +55 from the pegRNA and expressed this sgRNA from the OsU3 promoter using the polycistronic tRNA-gRNA system (Butt et al., 2017; Xie et al., 2015) (Figure 1a).”
MethodRegulation of DNA double-strand break repair pathway choice
Shrivastav, M., De Haro, L. P., & Nickoloff, J. A. (2008) · Cell Research · doi:10.1038/cr.2007.111 · Cited by 1,329 · see citing works
Prime genome editing in rice and wheat
Lin, Q., Zong, Y., Xue, C., Wang, S., Jin, S., Zhu, Z., Wang, Y., Anzalone, A. V., Raguram, A., Doman, J. L., et al. (2020) · Nature Biotechnology · doi:10.1038/s41587-020-0455-x · Cited by 873 · cited 1× · see citing works
“While this work was prepared for publication, similar findings were reported in pants (Li et al., 2020; Lin et al., 2020; Tang et al., 2020).”
ResultPrecise Modifications of Both Exogenous and Endogenous Genes in Rice by Prime Editing
Li, H., Li, J., Chen, J., Yan, L., & Xia, L. (2020) · Molecular Plant · doi:10.1016/j.molp.2020.03.011 · Cited by 202 · cited 1× · see citing works
“While this work was prepared for publication, similar findings were reported in pants (Li et al., 2020; Lin et al., 2020; Tang et al., 2020).”
ResultPlant Prime Editors Enable Precise Gene Editing in Rice Cells
Tang, X. D., Sretenovic, S., Ren, Q., Jia, X., Li, M., Fan, T., Yin, D., Xiang, S., Guo, Y., Liu, L., et al. (2020) · Molecular Plant · doi:10.1016/j.molp.2020.03.010 · Cited by 194 · cited 1× · see citing works
“While this work was prepared for publication, similar findings were reported in pants (Li et al., 2020; Lin et al., 2020; Tang et al., 2020).”
ResultAli, Z., Shami, A., Sedeek, K., Kamel, R., Alhabsi, A., Tehseen, M., Hassan, N., Butt, H., Kababji, A. M., Hamdan, S. M., et al. (2020) · Communications Biology · doi:10.1038/s42003-020-0768-9 · Cited by 140 · cited 1× · see citing works
“In plants, homology-directed repair is inefficient, limiting our ability to make precise edits of the DNA sequence (Ali et al., 2020; Butt et al., 2017).”
BackgroundSelf-citationEfficient CRISPR/Cas9-Mediated Genome Editing Using a Chimeric Single-Guide RNA Molecule
Butt, H., Eid, A., Ali, Z., Atia, M. A. M., Mokhtar, M. M., Hassan, N., Lee, C. M., Bao, G., & Mahfouz, M. M. (2017) · Frontiers in Plant Science · doi:10.3389/fpls.2017.01441 · Cited by 130 · cited 4× · see citing works
“A single amino acid change (W548L) in ALS results in a BS-resistant phenotype (Butt et al., 2017).”
BackgroundMethodInfluentialSelf-citation
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Precise plant genome editing using base editors and prime editors
Molla, K. A., Sretenovic, S., Bansal, K. C., & Qi, Y. (2021) · Nature Plants · doi:10.1038/s41477-021-00991-1 · Cited by 387 · see citing works
High-efficiency prime editing with optimized, paired pegRNAs in plants
Lin, Q., Jin, S., Zong, Y., Yu, H., Zhu, Z., Liu, G., Kou, L., Wang, Y., Qiu, J.-L., Li, J., et al. (2021) · Nature Biotechnology · doi:10.1038/s41587-021-00868-w · Cited by 325 · see citing works
Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize
Jiang, Y., Chai, Y., Lu, M., Han, X., Lin, Q., Zhang, Y., Zhang, Q., Zhou, Y., Wang, X., Gao, C., et al. (2020) · Genome biology · doi:10.1186/s13059-020-02170-5 · Cited by 281 · see citing works
“More recently, seven groups reported 40 applications of prime editors (PE) in rice and wheat, and six groups achieved prime-edited rice lines 41 [2-8].”
BackgroundTargeted genome-modification tools and their advanced applications in crop breeding
Li, B., Sun, C., Li, J., & Gao, C. (2024) · Nature Reviews Genetics · doi:10.1038/s41576-024-00720-2 · Cited by 259 · see citing works
Application of CRISPR/Cas9-mediated gene editing for abiotic stress management in crop plants
Kumar, M., Prusty, M. R., Pandey, M. K., Singh, P. K., Bohra, A., Guo, B. Z., & Varshney, R. K. (2023) · Frontiers in Plant Science · doi:10.3389/fpls.2023.1157678 · Cited by 245 · see citing works
“sativa OsTB1 AF322143 AgrobacteriumMediated (Butt et al., 2020)”
Next-Generation Breeding Strategies for Climate-Ready Crops
Razzaq, A., Kaur, P., Akhter, N., Wani, S. H., & Saleem, F. (2021) · Frontiers in Plant Science · doi:10.3389/fpls.2021.620420 · Cited by 209 · see citing works
Precise genome modification in tomato using an improved prime editing system
Lu, Y., Tian, Y., Shen, R., Yao, Q., Zhong, D., Zhang, X., & Zhu, J. (2020) · Plant Biotechnology Journal · doi:10.1111/pbi.13497 · Cited by 164 · see citing works
“Several research groups have adopted this method for use in monocotyledonous plants, including rice andwheat (Butt et al., 2020; Hua et al., 2020; Li et al., 2020; Lin et al., 2020; Tang et al., 2020; Xu et al., 2020).”
MethodCRISPR ribonucleoprotein-mediated genetic engineering in plants
Zhang, Y., Iaffaldano, B. J., & Qi, Y. (2021) · Plant Communications · doi:10.1016/j.xplc.2021.100168 · Cited by 169 · see citing works
“Cas proteins can be further engineered to recruit effector protein domains in order to achieve base editing (Kim, 2018), prime editing (Li et al., 2020b; Butt et al., 2020; Lin et al., 2020; Lu et al., 2020; Tang et al., 2020; Xu et al., 2020), epigenome editing (GallegoBartolomé et al.”
BackgroundConstruct design for CRISPR/Cas-based genome editing in plants
Hassan, M. M., Zhang, Y., Yuan, G., De, K., Chen, J., Muchero, W., Tuskan, G. A., Qi, Y., & Yang, X. (2021) · Trends in Plant Science · doi:10.1016/j.tplants.2021.06.015 · Cited by 136 · see citing works
CRISPR–Cas applications in agriculture and plant research
Tuncel, A., Pan, C., Clem, J. S., Liu, D., & Qi, Y. (2025) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-025-00834-3 · Cited by 139 · see citing works
“This variability necessitates the optimization of delivery methods and development of more robust”
Wang, Y., Zafar, N., Ali, Q., Manghwar, H., Wang, G., Yu, L., Ding, X. L., Ding, F., Hong, N., Wang, G., et al. (2022) · Cells · doi:10.3390/cells11233928 · Cited by 135 · see citing works
Genome editing for plant research and crop improvement
Zhan, X., Lu, Y., Zhu, J., & Botella, J. R. (2020) · Journal of Integrative Plant Biology · doi:10.1111/jipb.13063 · Cited by 121 · see citing works
Prime Editing in the model plant Physcomitrium patens and its potential in the tetraploid potato
Perroud, P., Guyon‐Debast, A., Veillet, F., Kermarrec, M.-P., Chauvin, L., Chauvin, J.-É., Gallois, J., & Nogué, F. (2021) · Plant Science · doi:10.1016/j.plantsci.2021.111162 · Cited by 123 · see citing works
CRISPR-Based Crop Improvements: A Way Forward to Achieve Zero Hunger
Ahmad, S., Tang, L., Shahzad, R., Mawia, A. M., Rao, G. S., Jamil, S., Chen, W., Sheng, Z., Shao, G., Wei, X., et al. (2021) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.1c02653 · Cited by 120 · see citing works
Self-citationAdvances in Crop Breeding Through Precision Genome Editing
Nerkar, G., Devarumath, S., Purankar, M. V., Kumar, A., Valarmathi, R., Devarumath, R. M., & Appunu, C. (2022) · Frontiers in Genetics · doi:10.3389/fgene.2022.880195 · Cited by 121 · see citing works
“Prime editing has been reported in cereal crops (Butt et al., 2020; Lin et al., 2020; Tang et al., 2020; Fiaz et al., 2021b) and has an advantage of fewer bystander mutations compared to base editing and also less restricted to PAM availability compared to the other genome editing methods (Anzalone et al.”
BackgroundPresent and future prospects for wheat improvement through genome editing and advanced technologies
Li, S., Zhang, C., Li, J., Yan, L., Wang, N., & Xia, L. (2021) · Plant Communications · doi:10.1016/j.xplc.2021.100211 · Cited by 118 · see citing works
“PE holds great promise for the introduction of genome modifications such as substitutions, insertions, and deletions that improve agronomic traits, and the feasibility and efficacy of prime editors for precise genome editing in cereal crops and tomato have therefore been investigated (Lu et al., 2020b; Butt et al., 20…”
BackgroundThe Development of Herbicide Resistance Crop Plants Using CRISPR/Cas9-Mediated Gene Editing
Dong, H., Huang, Y., & Wang, K. (2021) · Genes · doi:10.3390/genes12060912 · Cited by 112 · see citing works
Targeted DNA insertion in plants
Dong, O. X., & Ronald, P. C. (2021) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2004834117 · Cited by 106 · see citing works
Exploring and exploiting genetics and genomics for sweetpotato improvement: Status and perspectives
Yan, M., Nie, H., Wang, Y., Wang, X., Jarret, R. L., Zhao, J., Wang, H., & Yang, J. M. (2022) · Plant Communications · doi:10.1016/j.xplc.2022.100332 · Cited by 104 · see citing works
“…(Lu et al., 2017; Soyk et al., 18 Plant Communications 3, 100332, September 12 2022 ª 2022 Th 2017; Tang et al., 2017; D’Ambrosio et al., 2018; Ye et al., 2018; Miao et al., 2019; Zhang et al., 2019; Zhong et al., 2019; Butt et al., 2020; Zhang et al., 2020c; Li et al., 2021b; Zhan et al., 2021).”
A design optimized prime editor with expanded scope and capability in plants
Xu, W., Yang, Y., Yang, B., Krueger, C. J., Xiao, Q., Zhao, S., Zhang, L., Kang, G., Wang, F., Yi, H., et al. (2021) · Nature Plants · doi:10.1038/s41477-021-01043-4 · Cited by 101 · see citing works
Advances in Delivery Mechanisms of CRISPR Gene-Editing Reagents in Plants
Laforest, L. C., & Nadakuduti, S. S. (2022) · Frontiers in Genome Editing · doi:10.3389/fgeed.2022.830178 · Cited by 101 · see citing works
Nadakuduti, S. S., & Enciso-Rodríguez, F. E. (2021) · Frontiers in Plant Science · doi:10.3389/fpls.2020.637159 · Cited by 98 · see citing works
Precise genome engineering in Drosophila using prime editing
Bosch, J. A., Birchak, G., & Perrimon, N. (2020) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2021996118 · Cited by 94 · see citing works
Plant base editing and prime editing: The current status and future perspectives
Li, J., Zhang, C., He, Y., Li, S., Yan, L., Li, Y., Zhu, Z., & Xia, L. (2022) · Journal of Integrative Plant Biology · doi:10.1111/jipb.13425 · Cited by 93 · see citing works
Base Editing in Plants: Applications, Challenges, and Future Prospects
Azameti, M. K., & Dauda, W. P. (2021) · Frontiers in Plant Science · doi:10.3389/fpls.2021.664997 · Cited by 88 · see citing works
Rahman, S. U., McCoy, E., Raza, G., Ali, Z., Mansoor, S., & Amin, I. U. (2022) · Molecular Biotechnology · doi:10.1007/s12033-022-00456-6 · Cited by 86 · see citing works
Genome editing in cereal crops: an overview
Matres, J. M., Hilscher, J., Datta, A., Armario-Nájera, V., Baysal, C., He, W., Huang, X., Zhu, C., کامران, ر. و., Trijatmiko, K. R., et al. (2021) · Transgenic Research · doi:10.1007/s11248-021-00259-6 · Cited by 88 · see citing works
“Butt et al. (2020) reported 0.26 to 2% efficiency of prime editing in the rice gene ACETOLACTATE SYNTHASE (OsALS) involved in herbicide tolerance.”
BackgroundMethodInfluentialBi-PE: bi-directional priming improves CRISPR/Cas9 prime editing in mammalian cells
Tao, R. X., Wang, Y., Jiao, Y., Hu, Y. F., Li, L., Jiang, L., Zhou, L., Qu, J., Chen, Q., & Yao, S. (2022) · Nucleic Acids Research · doi:10.1093/nar/gkac506 · Cited by 83 · see citing works
A detailed landscape of CRISPR-Cas-mediated plant disease and pest management
Karmakar, S., Das, P., Panda, D., Xie, K., Baig, M. J., & Molla, K. A. (2022) · Plant Science · doi:10.1016/j.plantsci.2022.111376 · Cited by 83 · see citing works
CRISPR-Cas: A robust technology for enhancing consumer-preferred commercial traits in crops
Verma, V., Kumar, A., Partap, M., Thakur, M., & Bhargava, B. (2023) · Frontiers in Plant Science · doi:10.3389/fpls.2023.1122940 · Cited by 83 · see citing works
“Multiple independent studies have shown that PE has a poor effectiveness for modifying the genome of plants (Xu et al., 2019; Butt et al., 2020; Jiang et al., 2020; Lin et al., 2020; Lu et al., 2020; Tang et al., 2021).”
BackgroundXia, X., Cheng, X., Li, R., Yao, J., Li, Z., & Cheng, Y. (2021) · Theoretical and Applied Genetics · doi:10.1007/s00122-021-03874-3 · Cited by 78 · see citing works
Novel CRISPR/Cas applications in plants: from prime editing to chromosome engineering
Huang, T., & Puchta, H. (2021) · Transgenic Research · doi:10.1007/s11248-021-00238-x · Cited by 75 · see citing works
Ahmar, S., Hensel, G., & Gruszka, D. (2023) · Biotechnology Advances · doi:10.1016/j.biotechadv.2023.108248 · Cited by 79 · see citing works
CRISPR/Cas9-gene editing approaches in plant breeding
Saini, H., Thakur, R., Gill, R., Tyagi, K., & Goswami, M. (2023) · GM crops & food · doi:10.1080/21645698.2023.2256930 · Cited by 64 · see citing works
Herbicide Resistance: Another Hot Agronomic Trait for Plant Genome Editing
Hussain, A., Ding, X. L., Alariqi, M., Manghwar, H., Hui, F., Li, Y., Cheng, J., Wu, C., Cao, J., & Jin, S. (2021) · Plants · doi:10.3390/plants10040621 · Cited by 66 · see citing works
“More importantly, genome editing, specially programmable base editing, presents heritable, targeted modifications leading to transgene-free HR crops [40,115,122] that are genetically non-distinguishable to that of the plants created by traditional mutagenesis [151].”
BackgroundMethodInfluentialApplying gene editing to tailor precise genetic modifications in plants
Eck, J. M. V. (2020) · Journal of Biological Chemistry · doi:10.1074/jbc.rev120.010850 · Cited by 68 · see citing works
The CRISPR/Cas9 revolution continues: From base editing to prime editing in plant science
Li, Y., Li, W., & Li, J. (2021) · Journal of genetics and genomics/Journal of Genetics and Genomics · doi:10.1016/j.jgg.2021.05.001 · Cited by 63 · see citing works
A CRISPR ‐based lateral flow assay for plant genotyping and pathogen diagnostics
Sánchez, E., Ali, Z., Islam, T., et al. (2022) · Plant Biotechnology Journal · doi:10.1111/pbi.13924 · Cited by 60 · see citing works
Self-citationCRISPR/Cas systems: opportunities and challenges for crop breeding
Biswas, S., Zhang, D., & Shi, J. (2021) · Plant Cell Reports · doi:10.1007/s00299-021-02708-2 · Cited by 58 · see citing works
“2020), to achieve stable edited lines with desired edits in both exogenous and endogenous genes (Butt et al., 2020); Li et al.”
BackgroundBiswas, S., Bridgeland, A., Irum, S., Thomson, M. J., & Septiningsih, E. M. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23179809 · Cited by 59 · see citing works
Spelling Changes and Fluorescent Tagging With Prime Editing Vectors for Plants
Wang, L., Kaya, H. B., Zhang, N., Rai, R., Willmann, M. R., Carpenter, S. C. D., Read, A. C., Martin, F., Fei, Z., Leach, J. E., et al. (2021) · Frontiers in Genome Editing · doi:10.3389/fgeed.2021.617553 · Cited by 57 · see citing works
“In contrast to results in mammalian cells (Anzalone et al., 2019), PE3 did not increase editing efficiency in plants relative to PE2 (Butt et al., 2020; Lin et al., 2020; Veillet et al., 2020; Xu et al., 2020a).”
BackgroundInfluentialImprovement of base editors and prime editors advances precision genome engineering in plants
Hua, K., Han, P., & Zhu, J. (2021) · PLANT PHYSIOLOGY · doi:10.1093/plphys/kiab591 · Cited by 61 · see citing works
“346 The prime editing system has been tested in several plant species (Butt et al., 2020; 347 Hua et al., 2020a; Jiang et al., 2020; Li et al., 2020b; Lin et al., 2020; Lu et al., 2020; 348 Tang et al., 2020; Wang et al., 2021a; Xu et al., 2020a; Xu et al., 2020c).”
MethodJin, S., Lin, Q., Gao, Q., & Gao, C. (2022) · Nature Protocols · doi:10.1038/s41596-022-00773-9 · Cited by 56 · see citing works
Progress in gene editing tools, implications and success in plants: a review
Bhuyan, S. J., Kumar, M., Devde, P. R., Rai, A. C., Mishra, A. K., Singh, P. K., & Siddique, K. H. M. (2023) · Frontiers in Genome Editing · doi:10.3389/fgeed.2023.1272678 · Cited by 59 · see citing works
Precision genome editing in the eye
Suh, S., Choi, E. H., Raguram, A., Liu, D. R., & Palczewski, K. (2022) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2210104119 · Cited by 55 · see citing works
Kocsisova, Z., & Coneva, V. (2023) · Frontiers in Genome Editing · doi:10.3389/fgeed.2023.1209586 · Cited by 59 · see citing works
Integrating machine learning and genome editing for crop improvement
Chen, L., Liu, G., & Zhang, T. (2024) · aBIOTECH · doi:10.1007/s42994-023-00133-5 · Cited by 58 · see citing works
“CRISPR-derived technologies, like base editing and prime editing, have been successfully applied in targeted improvement of various traits across a broad spectrum of crops (Butt et al. 2020; Li et al. 2018; Mao et al. 2019).”
BackgroundCrop bioengineering via gene editing: reshaping the future of agriculture
Atia, M. A. M., Jiang, W., Sedeek, K., Butt, H., & Mahfouz, M. M. (2024) · Plant Cell Reports · doi:10.1007/s00299-024-03183-1 · Cited by 57 · see citing works
“Thus far, prime editing has been applied to many crops, including rice, wheat, corn, and tomato (Butt et al. 2020; Jiang et al. 2020; Lin et al. 2020; Lu et al. 2021).”
BackgroundSelf-citationPrecision Breeding Made Real with CRISPR: Illustration through Genetic Resistance to Pathogens
Veillet, F., Durand, M., Kroj, T., Césari, S., & Gallois, J. (2020) · Plant Communications · doi:10.1016/j.xplc.2020.100102 · Cited by 58 · see citing works
“This indicates that nicking the non-edited strand does not necessarily increase prime editing efficiency in plants (Hua et al., 2020a; Xu et al., 2020a; Butt et al., 2020; Lin et al., 2020).”
BackgroundKondo, K., & Taguchi, C. (2022) · Food Safety · doi:10.14252/foodsafetyfscj.d-21-00016 · Cited by 49 · see citing works
Plant genome editing: ever more precise and wide reaching
Sukegawa, S., Saika, H., & Toki, S. (2021) · The Plant Journal · doi:10.1111/tpj.15233 · Cited by 47 · see citing works
Ali, Q., Yu, C., Hussain, A., Ali, M., Ahmar, S., Sohail, M. A., Riaz, M., Ashraf, M. F., Abdalmegeed, D., Wang, X., et al. (2022) · Frontiers in Plant Science · doi:10.3389/fpls.2022.860281 · Cited by 50 · see citing works
Carotenoid Biofortification of Crops in the CRISPR Era
Zheng, X., Kuijer, H. N. J., & Al‐Babili, S. (2020) · Trends in biotechnology · doi:10.1016/j.tibtech.2020.12.003 · Cited by 46 · see citing works
CRISPR-Cas System, a Possible “Savior” of Rice Threatened by Climate Change: An Updated Review
Shaheen, N., Ahmad, S., Alghamdi, S. S., Rehman, H. M., Javed, M. A., Tabassum, J., & Shao, G. (2023) · Rice · doi:10.1186/s12284-023-00652-1 · Cited by 49 · see citing works
“…Japan, and USA) that have allowed GE tools for crops’ improvements and exempted the CRISPR-edited plants from GMO’s legislations because they possess mutations or genetic changes as similar as they can happen in conventional breeding or natural populations (Caccamo 2023; Schmidt et al. 2020) (Fig.”
BackgroundSattar, M. N., Iqbal, Z., Al-Khayri, J. M., & Jain, S. M. (2021) · Plants · doi:10.3390/plants10071347 · Cited by 42 · see citing works
Butt, H., Bazin, J., Prasad, K. V. S. K., et al. (2022) · Cells · doi:10.3390/cells11111796 · Cited by 41 · see citing works
Self-citationPrime editing: Mechanism insight and recent applications in plants
Vu, T. V., Nguyen, N. T., Kim, J., Hong, J. C., & Kim, J. (2023) · Plant Biotechnology Journal · doi:10.1111/pbi.14188 · Cited by 42 · see citing works
“…PE performance, the pioneering PE works in plants have inspired many plant groups to study and apply PE for precision plant breeding in monocots (Butt et al ., 2020; Hua et al ., 2020; Jiang et al ., 2020, 2022b; Li et al ., 2020a, 2022a, 2022b, 2022e, 2023b; Liang et al ., 2023; Lin et al .,…”
BackgroundXiong, X., Li, Z., Liang, J., Liu, K., Li, C., & Li, J.-F. (2022) · Nucleic Acids Research · doi:10.1093/nar/gkac166 · Cited by 41 · see citing works
CRISPR/Cas9 is a powerful tool for precise genome editing of legume crops: a review
Rasheed, A., Barqawi, A. A., Mahmood, A., Nawaz, M., Shah, A. N., Bay, D. H., Alahdal, M. A. Y., Hassan, M., & Qari, S. H. (2022) · Molecular Biology Reports · doi:10.1007/s11033-022-07529-4 · Cited by 40 · see citing works
Advancements and prospects of CRISPR/Cas9 technologies for abiotic and biotic stresses in sugar beet
Misra, V., Mall, A. K., Pandey, H., Srivastava, S., & Sharma, A. (2023) · Frontiers in Genetics · doi:10.3389/fgene.2023.1235855 · Cited by 43 · see citing works
Prime editing in plants: prospects and challenges
Vats, S., Kumar, J., Sonah, H., Zhang, F., & Deshmukh, R. K. (2024) · Journal of Experimental Botany · doi:10.1093/jxb/erae053 · Cited by 42 · see citing works
Prime Editing: An All-Rounder for Genome Editing
Lu, C., Kuang, J., Shao, T., Xie, S., Li, M., Zhu, L., & Zhu, L. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23179862 · Cited by 38 · see citing works
Mohamed, H. I., Khan, A. R., & Basıt, A. (2024) · Journal of soil science and plant nutrition · doi:10.1007/s42729-024-01778-x · Cited by 42 · see citing works
Derepression of specific miRNA-target genes in rice using CRISPR/Cas9
Lin, Y., Zhu, Y., Cui, Y., Chen, R., Chen, Z., Li, G., Fan, M., Chen, J., Li, Y., Guo, X., et al. (2021) · Journal of Experimental Botany · doi:10.1093/jxb/erab336 · Cited by 37 · see citing works
Balakrishnan, R., Manna, M., Begum, T. N., et al. (2024) · Physiology and Molecular Biology of Plants · doi:10.1007/s12298-024-01423-y · Cited by 33 · see citing works
Synthetic evolution of herbicide resistance using a T7 RNAP–based random DNA base editor
Butt, H., Moreno, J. L., & Mahfouz, M. M. (2022) · Life Science Alliance · doi:10.26508/lsa.202201538 · Cited by 34 · see citing works
“Ideally, such modifications could be introduced into the rice 373 genome via HDR-based technologies such as prime editing, RNA-templated DNA repair, 374 or Cas9-VirD2 fusion-mediated repair [42, 46-48].”
BackgroundSelf-citationPrecision Genome Engineering for the Breeding of Tomatoes: Recent Progress and Future Perspectives
Vu, T. V., Das, S., Tran, M. T., Hong, J. C., & Kim, J. (2020) · Frontiers in Genome Editing · doi:10.3389/fgeed.2020.612137 · Cited by 35 · see citing works
“Thus, there is a research opportunity for improvement for further applications in plants (Butt et al., 2020; Hua et al., 2020).”
BackgroundPrime Editing for Inherited Retinal Diseases
da Costa, B. L., Levi, S. R., Eulau, E., Tsai, Y.-T., & Quinn, P. M. J. (2021) · Frontiers in Genome Editing · doi:10.3389/fgeed.2021.775330 · Cited by 32 · see citing works
“However, additional studies demonstrated relatively lower rates of editing in hiPSCs, embryos, animals and plants, suggesting that there is large variability in the editing efficiency of this technique (Butt et al., 2020; Hua et al., 2020; Lin et al., 2020; Liu et al., 2020; Sürün et al., 2020).”
BackgroundApplication of CRISPR/Cas system in cereal improvement for biotic and abiotic stress tolerance
Maharajan, T., Krishna, T. P. A., Rakkammal, K., Ceasar, S. A., & Ramesh, M. (2022) · Planta · doi:10.1007/s00425-022-04023-w · Cited by 32 · see citing works
Promoter editing for the genetic improvement of crops
Shi, L., Su, J., Cho, M., Song, H., Dong, O. X., Liang, Y., & Zhang, Z. (2023) · Journal of Experimental Botany · doi:10.1093/jxb/erad175 · Cited by 33 · see citing works
Thomson, M. J., Biswas, S., Τσακιρπάλογλου, Ν., & Septiningsih, E. M. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23126565 · Cited by 29 · see citing works
Kaul, T., Thangaraj, A., Jain, R., et al. (2024) · Plant Physiology and Biochemistry · doi:10.1016/j.plaphy.2024.108374 · Cited by 28 · see citing works
Application of CRISPR/Cas9 in Rapeseed for Gene Function Research and Genetic Improvement
Tian, Q. C., Li, B., Feng, Y., Zhao, W., Huang, J., & Chao, H. (2022) · Agronomy · doi:10.3390/agronomy12040824 · Cited by 29 · see citing works
Modern Plant Biotechnology: An Antidote against Global Food Insecurity
Animasaun, D. A., Adedibu, P. A., Shkryl, Y., Emmanuel, F. O., Tekutyeva, L., & Balabanova, L. (2023) · Agronomy · doi:10.3390/agronomy13082038 · Cited by 31 · see citing works
Breeding herbicide-resistant rice using CRISPR-Cas gene editing and other technologies
Luo, Q., & Liu, Y. (2024) · Plant Communications · doi:10.1016/j.xplc.2024.101172 · Cited by 27 · see citing works
Synthetic directed evolution in plants: unlocking trait engineering and improvement
Rao, G. S., Jiang, W., & Mahfouz, M. M. (2021) · Synthetic Biology · doi:10.1093/synbio/ysab025 · Cited by 27 · see citing works
Self-citationPlant genome editing: CRISPR, base editing, prime editing, and beyond
Xie, Y., Haq, S. I. U., Jiang, X., Zheng, D., Feng, N., Wang, W., He, J., & Qiu, Q. (2022) · Grassland Research · doi:10.1002/glr2.12034 · Cited by 27 · see citing works
Developing herbicide-resistant crops through genome editing technologies: A review
Kuang, Y., Yu, H., Qi, F., Zhou, X., Li, X., & Zhou, H. (2024) · Crop Protection · doi:10.1016/j.cropro.2024.106745 · Cited by 29 · see citing works
CRISPR-Cas9 and beyond: what’s next in plant genome engineering
Zess, E. K., & Begemann, M. B. (2021) · In Vitro Cellular & Developmental Biology - Plant · doi:10.1007/s11627-021-10185-1 · Cited by 25 · see citing works
“Several subsequent studies have reported varying efficiencies in rice and other species (Butt et al. 2020; Hua et al. 2020; Jiang et al. 2020; Veillet et al. 2020).”
BackgroundCorrection of Beta-Thalassemia IVS-II-654 Mutation in a Mouse Model Using Prime Editing
Zhang, H., Sun, R., Fei, J., Chen, H., & Lu, D. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23115948 · Cited by 26 · see citing works
Beyond the lab: future-proofing agriculture for climate resilience and stress management
Pehlivan, N., Altaf, M. T., Emamverdian, A., & Ghorbani, A. (2025) · Frontiers in Plant Science · doi:10.3389/fpls.2025.1565850 · Cited by 28 · see citing works
“Notably, prime editing has been used to modify key agronomic genes like ALS, IPA, and TB1, enhancing traits such as herbicide resistance and yield-related architecture (Butt et al., 2020).”
Singh, C., Kumar, R., Sehgal, H., Bhati, S., Singhal, T., Gayacharan, Nimmy, M. S., Yadav, R., Gupta, S. K., Abdallah, N. A., et al. (2023) · Frontiers in Genetics · doi:10.3389/fgene.2023.1085024 · Cited by 26 · see citing works
“The PE method has been used with a variety of plants (Xu R. et al., 2020; Butt et al., 2020; XuW. et al., 2020; Hua et al., 2020; Jiang et al., 2020; Li et al., 2020).”
MethodCRISPR-based genome editing technology and its applications in oil crops
He, J., Zhang, K., Tang, M., Zhou, W., Chen, L., Chen, Z., & Li, M. (2021) · Oil Crop Science · doi:10.1016/j.ocsci.2021.07.002 · Cited by 24 · see citing works
Li, Z., Ma, R., Liu, D., Wang, M., Zhu, T., & Deng, Y. (2022) · Plant Science · doi:10.1016/j.plantsci.2022.111400 · Cited by 23 · see citing works
Genome editing for vegetable crop improvement: Challenges and future prospects
Devi, R., Chauhan, S., & Dhillon, T. S. (2022) · Frontiers in Genetics · doi:10.3389/fgene.2022.1037091 · Cited by 24 · see citing works
“PE has been successfully applied in rice and wheat to generate stable edited lines with price gene edits (Butt et al., 2020; Li H. et al., 2020c).”
BackgroundDevelopment and application of prime editing in plants
Tingting, L., Jinpeng, Z., Xi, Y., Kejian, W., Yuchun, R., & Chun, W. (2023) · Rice Science · doi:10.1016/j.rsci.2023.07.005 · Cited by 21 · see citing works
Advances in engineering CRISPR-Cas9 as a molecular Swiss Army knife
Meaker, G. A., Hair, E. J., & Gorochowski, T. E. (2020) · Synthetic Biology · doi:10.1093/synbio/ysaa021 · Cited by 21 · see citing works
A New Era in Herbicide-Tolerant Crops Development by Targeted Genome Editing
Gosavi, G., Ren, B., Li, X., Zhou, X., Spetz, C., & Zhou, H. (2022) · ACS Agricultural Science & Technology · doi:10.1021/acsagscitech.1c00254 · Cited by 21 · see citing works
Research progress of base editing and prime editing tools based on the CRISPR/Cas system
Gao, H., Gao, S., Kan, G., Valentovich, L. N., & An, Y. (2025) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2025.102771 · Cited by 23 · see citing works
Karunarathne, S., Walker, E., Sharma, D., Li, C., & Han, Y. (2023) · Journal of Zhejiang University-SCIENCE B · doi:10.1631/jzus.b2200552 · Cited by 18 · see citing works
“For instance, the rice gene Oryza sativa acetolactate synthase ( OsALS ) was targeted to develop resistance to the herbicide bispy‐ ribac sodium (Butt et al., 2020).”
Lokya, V., Singh, S., Chaudhary, R., Jangra, A., & Tiwari, S. (2025) · Plant Molecular Biology · doi:10.1007/s11103-025-01600-x · Cited by 20 · see citing works
“…been exploited for several applications to edit single-nucleotide base pairs through BE (Kim 2018), base pair substitutions, and small insertions or deletions using PE (Li et al. 2020; Butt et al. 2020; Lin et al. 2020; Tang et al. 2020; Xu et al. 2020; Molla et al. 2021; Zhang et al. 2021a, b).”
Sophisticated CRISPR/Cas tools for fine-tuning plant performance
Capdeville, N., Merker, L., Schindele, P., & Puchta, H. (2020) · Journal of Plant Physiology · doi:10.1016/j.jplph.2020.153332 · Cited by 20 · see citing works
Dissecting Plant Gene Functions Using CRISPR Toolsets for Crop Improvement
Zhang, R., Li, B., Yang, Z.-G., Huang, J., Sun, W.-H., Bhanbhro, N., Liu, W., & Chen, K. (2022) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.2c01754 · Cited by 19 · see citing works
CRISPR–Cas technology towards improvement of abiotic stress tolerance in plants
Ahmad, S., Sheng, Z., Jalal, R. S., Tabassum, J., Ahmed, F. K., Hu, S., Shao, G., Wei, X., Abd-Elsalam, K. A., Hu, P., et al. (2021) · Elsevier eBooks · doi:10.1016/b978-0-12-821910-2.00021-7 · Cited by 19 · see citing works
Engineering rice Nramp5 modifies cadmium and manganese uptake selectivity using yeast assay system
Inoue, J., Teramoto, T., Kazama, T., & Nakamura, T. (2024) · Frontiers in Plant Science · doi:10.3389/fpls.2024.1482099 · Cited by 18 · see citing works
“Several prime editing applications have been reported in various plants (Butt et al., 2020; Lin et al., 2020; Zong et al., 2022).”
Shoot-root signal circuit: Phytoremediation of heavy metal contaminated soil
Bai, S., Han, X., & Feng, D. (2023) · Frontiers in Plant Science · doi:10.3389/fpls.2023.1139744 · Cited by 19 · see citing works
“This method has been applied to some plants (Butt et al., 2020; Hua et al., 2020; Li et al., 2020; Lu et al., 2021), and has obtained tools with higher prime-editing efficiency (Jiang et al.”
MethodSynthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications
Clauer, P. R., Nou, A., Toth, T., Yu, Q., Chemla, Y., Boo, A., Yoon, K. S., & Voigt, C. A. (2026) · Chemical Reviews · doi:10.1021/acs.chemrev.4c00687 · Cited by 20 · see citing works
High-efficiency retron-mediated single-stranded DNA production in plants
Jiang, W., Rao, G. S., Aman, R., et al. (2022) · Synthetic Biology · doi:10.1093/synbio/ysac025 · Cited by 17 · see citing works
“Prime editing uses a chimeric fusion protein of an RNA-guided DNA nickase and reverse transcriptase (M-MLV RT) to extend the genetic information engineered in an RNA template into the target sequence (22).”
BackgroundSelf-citationThe Obstacles and Potential Solution Clues of Prime Editing Applications in Tomato
Vu, T. V., Nguyen, N. T., Kim, J., et al. (2022) · BioDesign Research · doi:10.34133/bdr.0001 · Cited by 17 · see citing works
Bio-SCAN V2: A CRISPR/dCas9-based lateral flow assay for rapid detection of theophylline
Jiang, W., Aman, R. A., Ali, Z., et al. (2023) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2023.1118684 · Cited by 16 · see citing works
“Cas9 nickase (nCas9) and nuclease-dead Cas9 (dCas9) can be generated by mutating one or both of its endonuclease domains, respectively (Kleinjan et al., 2017; Butt et al., 2020; Volke et al., 2022).”
BackgroundSelf-citation
- Authors
- Butt, H., Rao, G. S., Sedeek, K., Aman, R., Kamel, R., & Mahfouz, M.
- Affiliations
- King Abdullah University of Science and Technology (SA)
- Corresponding author
- Mahfouz, Magdy
- Venue
- Plant Biotechnology Journal
- Publisher
- Wiley
- Published
- 2020-06-11
- Volume
- 18
- Issue
- 12
- Pages
- 2370-2372
- ISSN
- 1467-7644 (ISSN-L)
- 1467-7652
- PMC
- PMC7680537
- OpenAlex
- W3025159539
- Semantic Scholar
- 707270480172698cd9a447b013ee5db6c1cc92fa
- Cited by
- 220
- References
- 10
- Citations by year
- 2026: 26 · 2025: 33 · 2024: 32 · 2023: 28 · 2022: 51 · 2021: 38 · 2020: 12
- FWCI
- 10.42
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Plant Biotechnology Journal (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- King Abdullah University of Science and Technology Repository (King Abdullah University of Science and Technology) (submittedVersion, cc-by)
- Funders
- King Abdullah University of Science and Technology
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetically Modified Organisms Research
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Weed Control and Herbicide Applications
- Keywords
- prime editing, herbicide resistance, rice, CRISPR/Cas9, genome editing, homology-directed repair, plant genome editing
- MeSH terms
- Gene Editing, Oryza, Herbicide Resistance, CRISPR-Cas Systems
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