Oscillation of gap junction electrical coupling in the mouse pancreatic islets of Langerhans.
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1. Pancreatic beta-cells oscillate synchronously when grouped in islets. Coupling seems essential to maintain this oscillatory behaviour, as isolated cells are unable to oscillate. This allows the islet to be used as a model system for studying the role of coupling in the generation of oscillatory patterns. 2. Pairs of beta-cells were intracellularly recorded in islets. beta-Cells oscillated synchronously. Propagated voltage deflections were observed as a function of glucose concentration and of the distance between the recording electrodes. Space constants were smaller in the silent than in the active phases, suggesting a higher intercellular connection in the active phases. 3. Coupling coefficients and estimated coupling conductances were larger in the active than in the silent phases. 4. Coupling coefficients and coupling conductances changed dynamically and in phase with the membrane potential oscillations, pointing to an active modulation of the gap junctions. 5. We hypothesize a role for coupling in the generation of the oscillatory events, providing different levels of permeability dependent on the state of conductance during the oscillatory phases.
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The coupling conductance between two β-cells in an islet has been estimated as 180 pS, and that between a pair of isolated β-cells as 150-220 pS.
“The coupling conductance between two β-cells in an islet has been estimated as 180 pS (762), and that between a pair of isolated β-cells as 150-220 pS (17, 508).”
From Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men (2018) · cites this paper as [17]
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Self-citation
- Authors
- Andreu, E., Soria, B., & Sanchez-Andres, J. V.
- Affiliations
- University of Alicante (ES)
- Venue
- The Journal of Physiology
- Publisher
- Wiley
- Published
- 1997-02
- Volume
- 498
- Issue
- 3
- Pages
- 753-761
- ISSN
- 0022-3751 (ISSN-L)
- 1469-7793
- PMC
- PMC1159191
- OpenAlex
- W2023183151
- Cited by
- 58
- References
- 24
- Citations by year
- 2023: 1 · 2020: 3 · 2019: 2 · 2017: 2 · 2016: 1 · 2015: 4 · 2013: 3 · 2012: 2
- FWCI
- 1.07
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- The Journal of Physiology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Connexins and lens biology
- Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
- Life Sciences › Neuroscience › Cognitive Neuroscience › Neural dynamics and brain function
- Keywords
- electrical coupling, pancreatic beta cells, pancreatic islets, oscillations, coupling coefficient, membrane potential oscillations, space constant, intracellular recording
- MeSH terms
- Animals, Electrophysiology, Glucose, Islets of Langerhans, Membrane Potentials, Gap Junctions, Patch-Clamp Techniques, Mice, In Vitro Techniques
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