Cited by 1,449HIV Research and TreatmentVirus-based gene therapy researchHIV/AIDS drug development and treatment

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Identification of Host Proteins Required for HIV Infection Through a Functional Genomic Screen

Brass, Abraham L., Dykxhoorn, Derek M., Benita, Yair, Yan, Nan, Engelman, Alan, Xavier, Ramnik J., Lieberman, Judy, Elledge, Stephen J.·2008·Science

DOI 10.1126/science.1152725

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Abstract

HIV-1 exploits multiple host proteins during infection. We performed a large-scale small interfering RNA screen to identify host factors required by HIV-1 and identified more than 250 HIV-dependency factors (HDFs). These proteins participate in a broad array of cellular functions and implicate new pathways in the viral life cycle. Further analysis revealed previously unknown roles for retrograde Golgi transport proteins (Rab6 and Vps53) in viral entry, a karyopherin (TNPO3) in viral integration, and the Mediator complex (Med28) in viral transcription. Transcriptional analysis revealed that HDF genes were enriched for high expression in immune cells, suggesting that viruses evolve in host cells that optimally perform the functions required for their life cycle. This effort illustrates the power with which RNA interference and forward genetics can be used to expose the dependencies of human pathogens such as HIV, and in so doing identify potential targets for therapy.

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Host factors exploited by retroviruses
Goff, S. P. (2007) · Nature Reviews Microbiology · doi:10.1038/nrmicro1541 · Cited by 247

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10.1146/annurev.biochem.67.1.1 · DOI
10.1146/annurev.cellbio.20.010403.102350 · DOI
10.1128/JVI.79.18.11742-11751.2005 · DOI
10.1016/S1097-2765(03)00116-3 · DOI
10.1016/j.tibs.2005.03.011 · DOI
10.1016/j.tcb.2005.12.004 · DOI
10.1016/j.cell.2006.09.029 · DOI
10.1074/jbc.M511311200 · DOI
10.1093/glycob/cwj066 · DOI
10.1007/s00430-006-0016-z · DOI
10.1016/S0006-291X(86)80330-8 · DOI
10.1038/nrmicro1707 · DOI
10.1101/gad.1599207 · DOI
10.1111/j.1600-0854.2006.00395.x · DOI
10.1038/15658 · DOI
10.1074/jbc.M209120200 · DOI
10.1093/emboj/19.18.4885 · DOI
10.1091/mbc.11.1.305 · DOI
10.1091/mbc.e05-11-1002 · DOI
10.1093/emboj/20.21.5991 · DOI
10.1074/jbc.M209428200 · DOI
10.1091/mbc.e04-11-1023 · DOI
10.1074/jbc.275.11.7950 · DOI
10.1091/mbc.e02-02-0102 · DOI
10.1128/JVI.74.9.4404-4413.2000 · DOI
10.1016/j.virol.2004.03.005 · DOI
10.1128/JVI.74.14.6377-6385.2000 · DOI
10.1097/00002030-200404090-00002 · DOI
10.1016/S0166-3542(02)00130-4 · DOI
10.1016/j.tcb.2005.04.006 · DOI
10.1002/yea.1032 · DOI
10.1083/jcb.200612135 · DOI
10.1016/j.yexcr.2005.11.015 · DOI
10.1126/science.1143767 · DOI

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“From the initial selection, only 4 genes were previously identified in siRNA/ shRNA genomic screens as host factors required for HIV-1 replication (Brass et al., 2008; Konig et al., 2008; Yeung et al., 2009; Zhou et al., 2008).”
Background
Viral infection causes a shift in the self peptide repertoire presented by human MHC class I molecules
Spencer, C. T., Bezbradica, J. S., Ramos, M., Arico, C. D., Conant, S. B., Gilchuk, P., Gray, J. J., Zheng, M., Niu, X., Hildebrand, W. H., et al. (2015) · PROTEOMICS - CLINICAL APPLICATIONS · doi:10.1002/prca.201500106 · Cited by 30
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Background
Antiviral Stratagems against HIV-1 Using RNA Interference (RNAi) Technology
Vlachakis, D., Tsiliki, G., Pavlopoulou, A., Roubelakis, M. G., Tsaniras, S. C., & Κοσσίδα, Σ. (2013) · Evolutionary Bioinformatics · doi:10.4137/ebo.s11412 · Cited by 27
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“Thus, it is essential to complement physical interactome mapping with functional assays that identify host proteins whose perturbation significantly affects viral infection and replication (Brass et al., 2008; Konig et al., 2008, 2010; Krishnan et al., 2008; Karlas et al., 2010).”
Background
Genetic manipulation of myoblasts and a novel primary myosatellite cell culture system: comparing and optimizing approaches
Jackson, M. F., Hoversten, K. E., Powers, J. M., Trobridge, G. D., & Rodgers, B. D. (2012) · FEBS Journal · doi:10.1111/febs.12072 · Cited by 28
Multicohort genomewide association study reveals a new signal of protection against HIV-1 acquisition.
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Host Genes Important to HIV Replication and EvolutionPreprint
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“Genome-wide siRNA and shRNA screens have generated a comprehensive view of genes required for efficient viral replication (Brass et al. 2008; Konig et al. 2008; Zhou et al. 2008; Yeung et al. 2009).”
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“Accordingly, furin inhibitors have been shown to block infection by influenza virus (52) and HIV (36).”
BackgroundInfluential
Genomewide association study of a rapid progression cohort identifies new susceptibility alleles for AIDS (ANRS Genomewide Association Study 03).
Clerc, S. L., Limou, S., Coulonges, C., Carpentier, W., Dina, C., Taing, L., Delaneau, O., Labib, T., Sladek, R., Deveau, C., et al. (2009) · Journal of Infectious Diseases · doi:10.1086/605892 · Cited by 27
“P p 1.62 10 rs1020064 has been associated with differential expression of TGFBRAP1 in the Genevar database and of PPP2R3A [20] in the Dixon database (Figure 5). More strikingly, in the Dixon database the nonsynonymous SNP rs2241797 was significantly associated with the differential expression of several proteins (Figu…”
BackgroundInfluential
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“Host factors responsible for HIV-1 nuclear import, for example, the karyopherin TNPO3 and the nuclear pore proteins Nup153 and Nup358, were initially identified in genomewide RNAi screens as host factors essential for HIV-1 infection (Brass et al. 2008; Konig et al. 2008).”
HIV-1 usurps mixed-charge domain-dependent CPSF6 phase separation for higher-order capsid binding, nuclear entry and viral DNA integration
Jang, S., Bedwell, G. J., SINGH, S. P., Yu, H. J., Arnarson, B., Singh, P. K., Radhakrishnan, R., Douglas, A. W., Ingram, Z., Freniere, C., et al. (2024) · Nucleic Acids Research · doi:10.1093/nar/gkae769 · Cited by 28
“To assess the generalizability of these findings, we expanded the analysis to HeLa adenocarcinoma cells, which, like U2OS cells, are highly responsive to siRNA-mediated knockdowns ( 78 ).”
BackgroundSelf-citation
The Role of Capsid in HIV-1 Nuclear Entry
Guedán, A., Caroe, E. R., Barr, G. C. R., & Bishop, K. N. (2021) · Viruses · doi:10.3390/v13081425 · Cited by 29
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“Although HDFs are essential for HIV-1 replication, silencing of their expression is not lethal to the host cell.(26,27) Moreover, 10 transcripts of the double bromodomaincontaining protein (BRD2) were drastically downregulated in ART Tregs.”
Background
Advances in high-throughput methods for the identification of virus receptors
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Background
Normalizing for individual cell population context in the analysis of high-content cellular screens
Knapp, B. L., Rebhan, I., Kumar, A., Matula, P., Kiani, N. A., Binder, M., Erfle, H., Rohr, K., Eils, R., Bartenschlager, R. F. W., et al. (2011) · BMC Bioinformatics · doi:10.1186/1471-2105-12-485 · Cited by 28
“This has successfully been used in several large-scale screens, for example, focusing on genes involved in mitosis [1], immune response [2] or viral infection [3,4].”
Method
Role of β-Catenin/TCF-4 Signaling in HIV Replication and Pathogenesis: Insights to Informing Novel Anti-HIV Molecular Therapeutics
Henderson, L. J., & Al‐Harthi, L. (2011) · Journal of Neuroimmune Pharmacology · doi:10.1007/s11481-011-9266-7 · Cited by 26
“HIV/host interaction is complex, as evidenced by a high-throughput siRNA screen that identified hundreds of host factors that HIV requires for its propagation (Brass et al. 2008).”
Background
Determination of sample size in genome-scale RNAi screens
Zhang, X. D., & Heyse, J. (2009) · Bioinform. · doi:10.1093/bioinformatics/btp082 · Cited by 26
Chapter 5 Rab Proteins and Their Interaction Partners
Barnekow, A., Thyrock, A., & Kessler, D. (2009) · International review of cytology · doi:10.1016/s1937-6448(08)02005-4 · Cited by 23
Digoxin reveals a functional connection between HIV-1 integration preference and T-cell activation
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“CA also interacts with the mRNA processing factor CPSF6 and with the nuclear transport receptor Transportin 3 (TNPO3), which were shown to be important for HIV-1 pre- and post-nuclear entry steps [11,12,13,14,15].”
Background
Non-POU Domain-Containing Octamer-Binding Protein Negatively Regulates HIV-1 Infection in CD4 + T Cells
Gelais, C. S., Roger, J. C., & Wu, L. J. (2015) · AIDS Research and Human Retroviruses · doi:10.1089/aid.2014.0313 · Cited by 20
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Permissive and restricted virus infection of murine embryonic stem cells
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“Venn diagram was generated from supplementary data of [ 27 , 29-31 ] using Venny [72] .”
BackgroundMethod
Cellular RNA helicases and HIV-1: Insights from genome-wide, proteomic, and molecular studies
Chen, C., Liu, X., Boris‐Lawrie, K., Sharma, A., & Jeang, K.-T. (2012) · Virus Research · doi:10.1016/j.virusres.2012.06.022 · Cited by 25
“Over the past three years, four siRNA/shRNAbased genome-wide screenings for HIV-1 dependency factors have been performed (Brass et al., 2008; Konig et al., 2008; Yeung et al., 2009; Zhou et al., 2008a).”
Background
Human immunodeficiency virus type 1 envelope proteins traffic toward virion assembly sites via a TBC1D20/Rab1-regulated pathway
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Knockdown of MAP4 and DNAL1 Produces a Post-Fusion and Pre-Nuclear Translocation Impairment in HIV-1 Replication
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“Recent genome-wide siRNA screens aimed at identifying HIV-1 dependency factors have uncovered hundreds of cellular factors that may be required for HIV infection at various stages of the life cycle (Brass et al., 2008; Bushman et al., 2009; Konig et al., 2008).”
Association of polymorphisms in the LEDGF/p75 gene (PSIP1) with susceptibility to HIV-1 infection and disease progression
Madlala, P., Gijsbers, R., Christ, F., Hombrouck, A., Werner, L., Mlisana, K. P., An, P., Karim, S. S. A., Winkler, C. A., Debyser, Z., et al. (2011) · AIDS · doi:10.1097/qad.0b013e328349c693 · Cited by 28
“These findings suggest that SNP3C reduces the mRNA levels of LEDGF/p75 thereby inhibiting HIV-1 replication, a finding that is consistent with in-vitro knockdown studies [19,36].”
Result
Characterization of host genetic expression patterns in HIV-infected individuals with divergent disease progression.
Salgado, M., López-Romero, P., Callejas, S., López, M., Labarga, P., Dopazo, A., Soriano, V., & Rodés, B. (2011) · Virology · doi:10.1016/j.virol.2010.12.037 · Cited by 25
“These changes are generated by virus–host–cell interaction, as reported in recent studies that showed the virus needs around 200 host proteins to develop infection (Brass et al., 2008; König et al., 2008; Zhou et al., 2008; Bushman et al., 2009).”
BackgroundResult
Strand Transfer and Elongation of HIV-1 Reverse Transcription Is Facilitated by Cell Factors In Vitro
Warrilow, D., Warren, K., & Harrich, D. (2010) · PLoS ONE · doi:10.1371/journal.pone.0013229 · Cited by 25
“Therefore, it is not unexpected that recent studies using si/shRNA knockdown [1,2,3,4,5] and yeast two-hybrid screening implicate host cell factors in HIV reverse transcription [6,7,8].”
Result
Transduction of human primitive repopulating hematopoietic cells with lentiviral vectors pseudotyped with various envelope proteins.
Kim, Y.-S., Wielgosz, M. M., Hargrove, P. W., Kepes, S., Gray, J., Persons, D., & Nienhuis, A. (2010) · Molecular Therapy · doi:10.1038/mt.2010.48 · Cited by 25
Tat-SF1 Is Not Required for Tat Transactivation but Does Regulate the Relative Levels of Unspliced and Spliced HIV-1 RNAs
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“TZM-bl cells were used for replication-competent HIV-1 infection because they express endogenous CXCR4, transgenic CD4 and CCR5, and integrated Tat-dependent beta-galactosidase and luciferase reporter genes. They have also been shown to be susceptible to RNAi”
Method
Metabolite profiling studies in Saccharomyces cerevisiae: an assisting tool to prioritize host targets for antiviral drug screening
Schneider, K., Krömer, J., Wittmann, C., Alves-Rodrigues, I., Meyerhans, A., Díez, J., & Heinzle, E. (2009) · Microbial Cell Factories · doi:10.1186/1475-2859-8-12 · Cited by 25
Insights into cellular microRNAs and human immunodeficiency virus type 1 (HIV‐1)
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“One interpretation of this latter finding is that the virus has ‘‘learned’’ to repress the expression of virus-noxious miRNAs, while enhancing the expression of propitious miRNAs that upregulate protein factors that benefit HIV-1 replication (Lama and Planelles, 2007; Brass et al., 2008).”
Background
HIV-1 Antisense Protein of Different Clades Induces Autophagy and Associates with the Autophagy Factor p62
Liu, Z., Torresilla, C., Xiao, Y., Nguyen, P. T., Caté, C., Barbosa, K., Rassart, É., Cen, S., Bourgault, S., & Barbeau, B. (2018) · Journal of Virology · doi:10.1128/jvi.01757-18 · Cited by 28
Implication of Different HIV-1 Genes in the Modulation of Autophagy
Liu, Z., Xiao, Y., Torresilla, C., Rassart, É., & Barbeau, B. (2017) · Viruses · doi:10.3390/v9120389 · Cited by 26
“A genomic screen has, in fact, identified a number of autophagy-related host factors, including Atg7, Atg8, Atg12, and Atg16L2, which were essential for HIV-1 infection [33].”
Background
Identification of a Specific miRNA Profile in HIV-Exposed Seronegative Individuals
Yahyaei, S., Biasin, M., Saulle, I., Gnudi, F., Luca, M. D., Tasca, K. I., Trabattoni, D., Caputo, S. L., Mazzotta, F., & Clerici, M. (2016) · JAIDS Journal of Acquired Immune Deficiency Syndromes · doi:10.1097/qai.0000000000001070 · Cited by 23
Cyclophilin B enhances HIV-1 Infection
Deboer, J., Madson, C. J., & Belshan, M. (2016) · Virology · doi:10.1016/j.virol.2015.12.015 · Cited by 24
“Moreover, on global siRNA study identified CypB as an HIV dependency factor (Brass et al., 2008).”
Recent strategies and progress in identifying host factors involved in virus replication
König, R., & Stertz, S. (2015) · Current Opinion in Microbiology · doi:10.1016/j.mib.2015.06.001 · Cited by 29
Genome-wide search for the genes accountable for the induced resistance to HIV-1 infection in activated CD4+ T cells: apparent transcriptional signatures, co-expression networks and possible cellular processes
Xu, W., Han, M., Chen, D., Chen, L., Guo, Y. L., Willden, A., Liu, D., & Zhang, H. (2013) · BMC Medical Genomics · doi:10.1186/1755-8794-6-15 · Cited by 24
Evidence for selection at HIV host susceptibility genes in a West Central African human population
Zhao, K. X., Ishida, Y., Oleksyk, T. K., Winkler, C. A., & Roca, A. L. (2012) · BMC Evolutionary Biology · doi:10.1186/1471-2148-12-237 · Cited by 22
The Hematopoietic Cell-Specific Rho GTPase Inhibitor ARHGDIB/D4GDI Limits HIV Type 1 Replication
Watanabe, T., Urano, E., Miyauchi, K., Ichikawa, R., Hamatake, M., Misawa, N., Sato, K., Ebina, H., Koyanagi, Y., & Komano, J. (2012) · AIDS Research and Human Retroviruses · doi:10.1089/aid.2011.0180 · Cited by 24
Knockdown of the Cellular Protein LRPPRC Attenuates HIV-1 Infection
Schweitzer, C. J., Matthews, J. M., Madson, C. J., Donnellan, M. R., Cerny, R. L., & Belshan, M. (2012) · PLoS ONE · doi:10.1371/journal.pone.0040537 · Cited by 19
“Recently, three genome-wide RNAi screens have been published to identify HIV dependency factors [30,31,32].”
Background
TRIM5 and the Regulation of HIV-1 Infectivity
Luban, J. (2012) · Molecular Biology International · doi:10.1155/2012/426840 · Cited by 28
“Nup358/RanBP2, a nuclear pore protein that possesses a cyclophilin A domain also plays a role in HIV-1 infectivity [15, 17, 19, 43].”
Background
Associations between HIV and Human Pathways Revealed by Protein-Protein Interactions and Correlated Gene Expression Profiles
Chen, K.-C., Wang, T., & Chan, C. (2012) · PLoS ONE · doi:10.1371/journal.pone.0034240 · Cited by 23
Enhancers of Adeno-associated Virus AAV2 Transduction via High Throughput siRNA Screening
Wallen, A. J., Barker, G. A., Fein, D. E., Jing, H., & Diamond, S. L. (2011) · Molecular Therapy · doi:10.1038/mt.2011.4 · Cited by 24
Symplekin Specifies Mitotic Fidelity by Supporting Microtubule Dynamics
Cappell, K. M., Larson, B., Sciaky, N., & Whitehurst, A. W. (2010) · Molecular and Cellular Biology · doi:10.1128/mcb.00758-10 · Cited by 24
The Major Locus for Mouse Adenovirus Susceptibility Maps to Genes of the Hematopoietic Cell Surface-Expressed LY6 Family
Spindler, K., Welton, A. R., Lim, E., Duvvuru, S., Althaus, I., Imperiale, J. E., Daoud, A. I., & Chesler, E. (2010) · Journal of Immunology · doi:10.4049/jimmunol.0903363 · Cited by 24
The Interaction Between Lentiviral Integrase and LEDGF: Structural and Functional Insights
Hare, S., & Cherepanov, P. (2009) · Viruses · doi:10.3390/v1030780 · Cited by 26
Silencing cytokeratin 18 gene inhibits intracellular replication of Trypanosoma cruzi in HeLa cells but not binding and invasion of trypanosomes
Claser, C., Curcio, M., de Mello, S., Silveira, E. V., Monteiro, H. P., & Rodrigues, M. M. (2008) · BMC Cell Biology · doi:10.1186/1471-2121-9-68 · Cited by 24
“All strains were molecularly typed based on small subunit rDNA sequences to confirm their origin [ 35 ].”
BackgroundMethod
Intensive RNAi with lentiviral vectors in mammalian cells
Miest, T. S., Saenz, D. T., Meehan, A. M., Llano, M., & Poeschla, E. M. (2008) · Methods · doi:10.1016/j.ymeth.2008.11.001 · Cited by 21
DDX5 potentiates HIV-1 transcription as a co-factor of Tat
Sithole, N., Williams, C., Abbink, T., & Lever, A. (2020) · Retrovirology · doi:10.1186/s12977-020-00514-4 · Cited by 23
The mutation of Transportin 3 gene that causes limb girdle muscular dystrophy 1F induces protection against HIV-1 infection
Rodríguez-Mora, S., Wit, F. D., García‐Pérez, J., Bermejo, M., López‐Huertas, M. R., Mateos, E., Martí, P., Rocha, S., Vigón, L., Christ, F., et al. (2019) · PLoS Pathogens · doi:10.1371/journal.ppat.1007958 · Cited by 43
Host Restriction Factors and Human Immunodeficiency Virus (HIV-1): A Dynamic Interplay Involving All Phases of the Viral Life Cycle
D’Urbano, V., Crignis, E. D., & Re, M. C. (2018) · Current HIV Research · doi:10.2174/1570162x16666180817115830 · Cited by 31
Emerging Roles of N6-Methyladenosine on HIV-1 RNA Metabolism and Viral Replication
Riquelme-Barrios, S., Pereira-Montecinos, C., Valiente‐Echeverría, F., & Soto‐Rifo, R. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.00576 · Cited by 30
“While the role of the nuclear reader YTHDC1 on HIV-1 gene expression has not been investigated, a large-scale siRNA screen identified YTHDC2 as a host factor Frontiers in Microbiology | www.frontiersin.org 8 March 2018 | Volume 9 | Article 576 required for HIV-1 replication (Brass et al., 2008).”
Background
Species-specific vulnerability of RanBP2 shaped the evolution of SIV as it transmitted in African apes
Meyerson, N. R., Warren, C. J., Vieira, D. A. S. A., Diaz-Griferro, F., & Sawyer, S. L. (2018) · PLoS Pathogens · doi:10.1371/journal.ppat.1006906 · Cited by 27
“Depletion of RanBP2 negatively affects HIV-1 and HIV-2 infection and nuclear import [3] [4] [5] [6] [7] [8] [9] .”
Links between critical proteins drive the controllability of protein interaction networks
Wuchty, S., Boltz, T. A., & Küçük‐McGinty, H. (2017) · PROTEOMICS · doi:10.1002/pmic.201700056 · Cited by 21
RNA helicase MOV10 functions as a co-factor of HIV-1 Rev to facilitate Rev/RRE-dependent nuclear export of viral mRNAs
Huang, F., Zhang, J., Zhang, Y., Geng, G., Liang, J., Li, Y., Chen, J., Liu, C., & Zhang, H. (2015) · Virology · doi:10.1016/j.virol.2015.08.026 · Cited by 33
Novel Role of HSP40/DNAJ in the Regulation of HIV-1 Replication
Urano, E., Morikawa, Y., & Komano, J. (2013) · Journal of Acquired Immune Deficiency Syndromes · doi:10.1097/qai.0b013e31829a2ef8 · Cited by 23
A kinetic model for RNA-interference of focal adhesions
Hoffmann, M., & Schwarz, U. S. (2013) · BMC Systems Biology · doi:10.1186/1752-0509-7-2 · Cited by 31
The Role of TNPO3 in HIV-1 Replication
Diaz‐Griffero, F. (2012) · Molecular Biology International · doi:10.1155/2012/868597 · Cited by 19
“The role of TNPO3 in retroviral infection was initially discovered for HIV-1 [30]; however, more recent work has demonstrated that TNPO3 is also important for infection by HIV-2, simian lentiviruses, and, to a lesser extent, equine infectious anemia virus (EIAV) [31, 32, 37, 42] but not MLV or Feline immunodeficiency…”
Background
Identification of Mammalian Protein Quality Control Factors by High-Throughput Cellular Imaging
Pegoraro, G., Voss, T. C., Martin, S. E., Tuzmen, P., Guha, R., & Misteli, T. (2012) · PLoS ONE · doi:10.1371/journal.pone.0031684 · Cited by 19
Genetic correlates influencing immunopathogenesis of HIV infection
Sharma, G., Kaur, G., & Mehra, N. (2011) · The Indian journal of medical research · doi:10.4103/0971-5916.92623 · Cited by 23
Impaired Infectivity of Ritonavir-resistant HIV Is Rescued by Heat Shock Protein 90AB1
Joshi, P., & Stoddart, C. (2011) · Journal of Biological Chemistry · doi:10.1074/jbc.m111.248021 · Cited by 23
A SYSTEMS BIOLOGICAL VIEW OF INTRACELLULAR PATHOGENS
Beiting, D., & Roos, D. S. (2011) · Immunological Reviews · doi:10.1111/j.1600-065x.2010.00998.x · Cited by 23
BiologicalNetworks - tools enabling the integration of multi-scale data for the host-pathogen studies
Kozhenkov, S., Sedova, M., Dubinina, Y., Gupta, A., Ray, A., Ponomarenko, J., & Baitaluk, M. (2011) · BMC Systems Biology · doi:10.1186/1752-0509-5-7 · Cited by 23
“Additional experimental data sets on host-pathogen interactions integrated into our system include human interactomes used by Influenza virus, HIV, HCV, dengue virus and West Nile virus (WNV) (based on the results reported in [17,22-28]).”
Method
Evidence and controversies on the role of XMRV in prostate cancer and chronic fatigue syndrome
Menéndez‐Arias, L. (2010) · Reviews in Medical Virology · doi:10.1002/rmv.673 · Cited by 24
Transient inhibition of foot-and-mouth disease virus replication by siRNAs silencing VP1 protein coding region
Lv, K., Guo, Y.-J., Zhang, Y.-L., Wang, K.-Y., Li, K., Zhu, Y., & Sun, S.-H. (2008) · Research in Veterinary Science · doi:10.1016/j.rvsc.2008.10.011 · Cited by 23
Application of the proximity-dependent assay and fluorescence imaging approaches to study viral entry pathways.
Lipovsky, A., Zhang, W., Iwasaki, A., & DiMaio, D. (2015) · Methods in molecular biology · doi:10.1007/978-1-4939-2309-0_30 · Cited by 23
Role of Glycosphingolipids in Dendritic Cell-Mediated HIV-1 Trans-infection
Puryear, W. B., & Gummuluru, S. (2012) · Advances in experimental medicine and biology · doi:10.1007/978-1-4614-4433-6_5 · Cited by 26
“GSL depletion has been shown to inhibit viral fusion (Hug et al. 2000; Puri et al. 1998, 2004) and Gb3 and GM3 in particular can impact HIV-1 fusion with primary T cells (Brass et al. 2008).”
BackgroundInfluential
Toward a Durable Treatment of HIV-1 Infection Using RNA Interference
Eekels, J. J. M., & Berkhout, B. (2011) · Progress in molecular biology and translational science · doi:10.1016/b978-0-12-415795-8.00001-5 · Cited by 17
Trim69 is a microtubule regulator that acts as a pantropic viral inhibitor
Song, Y., Nguyen, X.-N., Kumar, A., da Silva, C., Picard, L., Etienne, L., & Cimarelli, A. (2022) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2211467119 · Cited by 23
Autophagy Intertwines with Different Diseases—Recent Strategies for Therapeutic Approaches
Ramesh, J., Ronsard, L., Gao, A., & Venugopal, B. (2019) · Diseases · doi:10.3390/diseases7010015 · Cited by 26
“Brass et al. 2008 [144,145] showed that in HIV infection mechanisms, certain autophagy genes, namely GABARAPL2, ATG12, ATG7, and ATG16L2, played a vital role.”
Background
HIV signaling through CD4 and CCR5 activates Rho family GTPases that are required for optimal infection of primary CD4+ T cells
Lucera, M. B., Fleissner, Z., Tabler, C. O., Schlatzer, D., Troyer, Z., & Tilton, J. (2017) · Retrovirology · doi:10.1186/s12977-017-0328-7 · Cited by 22
Identification of RNA helicases in human immunodeficiency virus 1 (HIV-1) replication – a targeted small interfering RNA library screen using pseudotyped and WT HIV-1
Williams, C., Abbink, T., Jeang, K., & Lever, A. (2015) · Journal of General Virology · doi:10.1099/vir.0.000092 · Cited by 22
“1Former address: Molecular Virology Section, Laboratory of Molecular Microbiology, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.”
BackgroundResultInfluential
Optimized Infectivity of the Cell-Free Single-Cycle Human Immunodeficiency Viruses Type 1 (HIV-1) and Its Restriction by Host Cells
Kim, J. H., Song, H., Austin, J. L., & Cheng, W. (2013) · PLoS ONE · doi:10.1371/journal.pone.0067170 · Cited by 21
“major barrier that restricts HIV virion infectivity [30].”
Background
A simple method for analyzing actives in random RNAi screens: introducing the “H Score” for hit nomination & gene prioritization
Bhinder, B., & Djaballah, H. (2012) · Combinatorial chemistry & high throughput screening · doi:10.2174/138620712803519671 · Cited by 22
“In 2008, three HIV host-virus interaction siRNA screens were published by Konig [5] and co-workers, Brass and co-workers [6], and Zhou and co-workers [7]; and in the following year an additional shRNA screen was also reported by Yeung and co-workers [8].”
Background
An overview of intracellular interactions between immunodeficiency viruses and their hosts
Bieniasz, P. D. (2012) · AIDS · doi:10.1097/qad.0b013e328353bd04 · Cited by 22
Viral proteomics: The emerging cutting-edge of virus research
Zhou, S. T., Liu, R., Zhao, X., Huang, C., & Wei, Y. (2011) · Science China Life Sciences · doi:10.1007/s11427-011-4177-7 · Cited by 25
The challenge of obtaining therapeutic levels of genetically modified hematopoietic stem cells in β‐thalassemia patients
Persons, D. (2010) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.2010.05581.x · Cited by 22
Phosphoproteome Analysis of Cells Infected with Adapted and Nonadapted Influenza A Virus Reveals Novel Pro- and Antiviral Signaling Networks
Weber, A., Dam, S., Saul, V. V., Kuznetsova, I., Müller, C., Fritz‐Wolf, K., Becker, K., Linne, U., Gu, H., Stokes, M. P., et al. (2019) · Journal of Virology · doi:10.1128/jvi.00528-19 · Cited by 24
G protein-coupled and ATP-sensitive inwardly rectifying potassium ion channels are essential for HIV entry
Dubey, R. C., Mishra, N., & Gaur, R. (2019) · Scientific Reports · doi:10.1038/s41598-019-40968-x · Cited by 18
The transporters SLC35A1 and SLC30A1 play opposite roles in cell survival upon VSV virus infection
Moskovskich, A., Goldmann, U., Kartnig, F., Lindinger, S., Konecka, J., Fiume, G., Girardi, E., & Superti-Furga, G. (2019) · Scientific Reports · doi:10.1038/s41598-019-46952-9 · Cited by 21
HIV-1 Fusion with CD4+ T cells Is Promoted by Proteins Involved in Endocytosis and Intracellular Membrane Trafficking
Marin, M., Kushnareva, Y., Mason, C. S., Chanda, S. K., & Melikyan, G. B. (2019) · Viruses · doi:10.3390/v11020100 · Cited by 24

Authors: Brass, A. L., Dykxhoorn, D. M., Benita, Y., Yan, N., Engelman, A., Xavier, R. J., Lieberman, J., & Elledge, S. J.
Affiliations: Brigham and Women's Hospital (US)
ORCID: Dykxhoorn, Derek M.
Benita, Yair
Yan, Nan
Engelman, Alan
Xavier, Ramnik J.
Lieberman, Judy
Elledge, Stephen J.
Venue: Science
Publisher: American Association for the Advancement of Science (AAAS)
Published: 2008-02-15
Volume: 319
Issue: 5865
Pages: 921-926
ISSN: 0036-8075 (ISSN-L)
1095-9203
DOI: 10.1126/science.1152725
OpenAlex: W2145848202
Cited by: 1,449
References: 0
Citations by year: 2026: 12 · 2025: 18 · 2024: 26 · 2023: 28 · 2022: 38 · 2021: 38 · 2020: 52 · 2019: 59 · 2018: 71 · 2017: 53 · 2016: 62 · 2015: 96 · 2014: 90 · 2013: 150 · 2012: 148
FWCI: 57.05
Type: journal article
Language: en
Open access: closed
Locations: Science (publishedVersion)
PubMed (publishedVersion)
Funders: Howard Hughes Medical Institute
National Institute of Allergy and Infectious Diseases (U19AI056900, AI052014, AI 062773, R01AI062773, R01AI052014, P30AI060354)
National Institute of Diabetes and Digestive and Kidney Diseases (P30DK040561)
Topics: Life Sciences › Immunology and Microbiology › Virology › HIV Research and Treatment
Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
Health Sciences › Medicine › Infectious Diseases › HIV/AIDS drug development and treatment
Keywords: HIV-1, host factors, host-virus interaction, Rab6, viral entry, karyopherins, TNPO3, viral integration, Mediator complex, MED28, viral transcription, antiviral drug targets
MeSH terms: Cytoskeletal Proteins, Humans, Proteins, Transcription, Genetic, Virus Replication, HIV-1, HIV Infections, Virus Integration, Computational Biology, rab GTP-Binding Proteins, Genomics, Karyopherins, Vesicular Transport Proteins, RNA Interference, RNA, Small Interfering, Cell Line, Tumor, Intracellular Signaling Peptides and Proteins, Virus Internalization, Human Immunodeficiency Virus Proteins, Host-Pathogen Interactions, Mediator Complex

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Identification of Host Proteins Required for HIV Infection Through a Functional Genomic Screen

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