Cited by 1,449HIV Research and TreatmentVirus-based gene therapy researchHIV/AIDS drug development and treatment

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Identification of Host Proteins Required for HIV Infection Through a Functional Genomic Screen

Brass, Abraham L., Dykxhoorn, Derek M., Benita, Yair, Yan, Nan, Engelman, Alan, Xavier, Ramnik J., Lieberman, Judy, Elledge, Stephen J.·2008·Science

DOI 10.1126/science.1152725

Open this paper in CoChat — free account

Abstract

HIV-1 exploits multiple host proteins during infection. We performed a large-scale small interfering RNA screen to identify host factors required by HIV-1 and identified more than 250 HIV-dependency factors (HDFs). These proteins participate in a broad array of cellular functions and implicate new pathways in the viral life cycle. Further analysis revealed previously unknown roles for retrograde Golgi transport proteins (Rab6 and Vps53) in viral entry, a karyopherin (TNPO3) in viral integration, and the Mediator complex (Med28) in viral transcription. Transcriptional analysis revealed that HDF genes were enriched for high expression in immune cells, suggesting that viruses evolve in host cells that optimally perform the functions required for their life cycle. This effort illustrates the power with which RNA interference and forward genetics can be used to expose the dependencies of human pathogens such as HIV, and in so doing identify potential targets for therapy.

Integrity signals

Registry

Crossref · 10.1126/science.1152725

Cites

0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Host factors exploited by retroviruses
Goff, S. P. (2007) · Nature Reviews Microbiology · doi:10.1038/nrmicro1541 · Cited by 247

Flagged first, then by citations

Show all 0 references

Research works printed but not linked: 34

10.1146/annurev.biochem.67.1.1 · DOI
10.1146/annurev.cellbio.20.010403.102350 · DOI
10.1128/JVI.79.18.11742-11751.2005 · DOI
10.1016/S1097-2765(03)00116-3 · DOI
10.1016/j.tibs.2005.03.011 · DOI
10.1016/j.tcb.2005.12.004 · DOI
10.1016/j.cell.2006.09.029 · DOI
10.1074/jbc.M511311200 · DOI
10.1093/glycob/cwj066 · DOI
10.1007/s00430-006-0016-z · DOI
10.1016/S0006-291X(86)80330-8 · DOI
10.1038/nrmicro1707 · DOI
10.1101/gad.1599207 · DOI
10.1111/j.1600-0854.2006.00395.x · DOI
10.1038/15658 · DOI
10.1074/jbc.M209120200 · DOI
10.1093/emboj/19.18.4885 · DOI
10.1091/mbc.11.1.305 · DOI
10.1091/mbc.e05-11-1002 · DOI
10.1093/emboj/20.21.5991 · DOI
10.1074/jbc.M209428200 · DOI
10.1091/mbc.e04-11-1023 · DOI
10.1074/jbc.275.11.7950 · DOI
10.1091/mbc.e02-02-0102 · DOI
10.1128/JVI.74.9.4404-4413.2000 · DOI
10.1016/j.virol.2004.03.005 · DOI
10.1128/JVI.74.14.6377-6385.2000 · DOI
10.1097/00002030-200404090-00002 · DOI
10.1016/S0166-3542(02)00130-4 · DOI
10.1016/j.tcb.2005.04.006 · DOI
10.1002/yea.1032 · DOI
10.1083/jcb.200612135 · DOI
10.1016/j.yexcr.2005.11.015 · DOI
10.1126/science.1143767 · DOI

Sorted by citations

Items 701–800 of 1,000

pH regulation in early endosomes and interferon-inducible transmembrane proteins control avian retrovirus fusion
Desai, T. M., Marin, M., Mason, C., & Melikyan, G. (2017) · Journal of Biological Chemistry · doi:10.1074/jbc.m117.783878 · Cited by 21
High-Throughput Small Interfering RNA Screening Identifies Phosphatidylinositol 3-Kinase Class II Alpha as Important for Production of Human Cytomegalovirus Virions
Polachek, W. S., Moshrif, H. F., Franti, M., Coen, D., Sreenu, V. B., & Strang, B. L. (2016) · Journal of Virology · doi:10.1128/jvi.01134-16 · Cited by 21
cSSMD: assessing collective activity for addressing off-target effects in genome-scale RNA interference screens
Zhang, X. D., Santini, F., Lacson, R., Marine, S., Wu, Q., Benetti, L., Yang, R., McCampbell, A., Berger, J. P., Toolan, D. M., et al. (2011) · Bioinformatics · doi:10.1093/bioinformatics/btr474 · Cited by 16
Antiviral RNAi: Translating Science Towards Therapeutic Success
Shah, P. S., & Schaffer, D. V. (2011) · Pharmaceutical Research · doi:10.1007/s11095-011-0549-8 · Cited by 23
Why and how to monitor the cost and evaluate the cost-effectiveness of HIV services in countries.
Beck, E., Santas, X. M., & Delay, P. (2008) · AIDS (London) · doi:10.1097/01.aids.0000327626.77597.fa · Cited by 21
The DNA methylation landscape of CD4+ T cells in oligoarticular juvenile idiopathic arthritis
Chavez, R. A., Chiaroni-Clarke, R. C., Martino, D., Munro, J. E., Allen, R. C., Akikusa, J., Ponsonby, A., Craig, J. M., Saffery, R., & Ellis, J. A. (2017) · Journal of Autoimmunity · doi:10.1016/j.jaut.2017.09.010 · Cited by 23
The Multifaceted Contributions of Chromatin to HIV-1 Integration, Transcription, and Latency
Crignis, E. D., & Mahmoudi, T. (2016) · International review of cytology · doi:10.1016/bs.ircmb.2016.08.006 · Cited by 23
“Functional genomic screens have highlighted the involvement of different nuclear transport components in HIV-1 replication (Brass et al., 2008; Christ et al., 2008; Konig et al., 2008; Ocwieja et al., 2011).”
Background
Interaction of drugs of abuse and microRNA with HIV: a brief review
Pilakka-Kanthikeel, S., & Nair, M. (2015) · Frontiers in Microbiology · doi:10.3389/fmicb.2015.00967 · Cited by 20
“…et al., 2010 Targets HDFs (LEDGF, Nup153 and ADAM10) Reduce viral integration Sisk et al., 2012 miR-17/92, miR-20a PCAF Inhibit HIV replication Brass et al., 2008 miR-15a, miR-15b, miR-16, miR-20a, miR-93, miR-106b Pur-α Low susceptibility to HIV infection in monocytes Woodward et al.,…”
Background
Genome-wide functional genomic and transcriptomic analyses for genes regulating sensitivity to vorinostat
Falkenberg, K., Gould, C. M., Johnstone, R., & Simpson, K. (2014) · Scientific Data · doi:10.1038/sdata.2014.17 · Cited by 20
Cellular and Biochemical Mechanisms of the Retroviral Restriction Factor SAMHD1
Wu, L. J. (2013) · ISRN Biochemistry · doi:10.1155/2013/728392 · Cited by 19
“Hindawi Publishing Corporation ISRN Biochemistry Volume 2013, Article ID 728392, 11 pages http://dx.doi.org/10.1155/2013/728392”
BackgroundInfluential
Time-Lapse Imaging of Neuroblastoma Cells to Determine Cell Fate upon Gene Knockdown
Batra, R., Harder, N., Gogolin, S., Diessl, N., Soons, Z. I. T. A., Jaeger-Schmidt, C., Lawerenz, C., Eils, R., Rohr, K., Westermann, F., et al. (2012) · PLoS ONE · doi:10.1371/journal.pone.0050988 · Cited by 19
RNA interference for viral infections.
Blake, S. J., Bokhari, F., & McMillan, N. (2012) · Current Drug Targets · doi:10.2174/138945012803530161 · Cited by 20
Selection of RNAi-based inhibitors for anti-HIV gene therapy
Knoepfel, S. A. (2012) · World Journal of Virology · doi:10.5501/wjv.v1.i3.79 · Cited by 17
Comparative high-throughput RNAi screening methodologies in C. elegans and mammalian cells
Simpson, K. J., Davis, G. M., & Boag, P. R. (2012) · New Biotechnology · doi:10.1016/j.nbt.2012.01.003 · Cited by 20
HIVToolbox, an Integrated Web Application for Investigating HIV
Sargeant, D. P., Deverasetty, S., Luo, Y., Baleta, A. V., Zobrist, S., Rathnayake, V. R., Russo, J. C., Vyas, J., Muesing, M. A., & Schiller, M. R. (2011) · PLoS ONE · doi:10.1371/journal.pone.0020122 · Cited by 21
Pulsed Stable Isotope Labeling of Amino Acids in Cell Culture Uncovers the Dynamic Interactions between HIV-1 and the Monocyte-Derived Macrophage
Kraft-Terry, S. D., Engebretsen, I. L., Bastola, D., Fox, H., Ciborowski, P., & Gendelman, H. (2011) · Journal of Proteome Research · doi:10.1021/pr200124j · Cited by 20
Huwe1, a novel cellular interactor of Gag-Pol through integrase binding, negatively influences HIV-1 infectivity.
Yamamoto, S. P., Okawa, K., Nakano, T., Sano, K., Ogawa, K., Masuda, T., Morikawa, Y., Koyanagi, Y., & Suzuki, Y. (2011) · Microbes and infection · doi:10.1016/j.micinf.2010.12.002 · Cited by 20
Host Proteome Research in HIV Infection
Zhang, L., Zhang, X., Ma, Q., & Zhou, H. (2010) · Genomics Proteomics & Bioinformatics · doi:10.1016/s1672-0229(10)60001-0 · Cited by 19
A Signaling Polypeptide Derived from an Innate Immune Adaptor Molecule Can Be Harnessed as a New Class of Vaccine Adjuvant
Kobiyama, K., Takeshita, F., Ishii, K. J., Koyama, S., Aoshi, T., Akira, S., Sakaue-Sawano, A., Miyawaki, A., Yamanaka, Y., Hirano, H., et al. (2009) · The Journal of Immunology · doi:10.4049/jimmunol.182.3.1593 · Cited by 17
JAK1 promotes HDV replication and is a potential target for antiviral therapy
Heuschkel, M. J., Bach, C., Meiss-Heydmann, L., Gerges, E., Felli, E., Giannone, F., Pessaux, P., Schuster, C., Lucifora, J., Baumert, T. F., et al. (2023) · Journal of Hepatology · doi:10.1016/j.jhep.2023.10.030 · Cited by 20
Emerging role of cyclophilin A in HIV-1 infection: from producer cell to the target cell nucleus
Padron, A., Prakash, P., Pandhare, J., Luban, J., Aiken, C., Balasubramaniam, M., & Dash, C. (2023) · Journal of Virology · doi:10.1128/jvi.00732-23 · Cited by 19
Genome-wide screens reveal Escherichia coli genes required for growth of T1-like phage LL5 and V5-like phage LL12
Piya, D., Lessor, L., Koehler, B., Stonecipher, A., Cahill, J., & Gill, J. (2018) · Scientific Reports · doi:10.1038/s41598-020-64981-7 · Cited by 19
Why Do We Need New Drug Classes for HIV Treatment and Prevention?
Waheed, A., & Tachedjian, G. (2016) · Current Topics in Medicinal Chemistry · doi:10.2174/1568026616999151013124606 · Cited by 19
Distinct Roles for CdtA and CdtC during Intoxication by Cytolethal Distending Toxins
Dixon, S. D., Huynh, M. M., Tamilselvam, B., Spiegelman, L., Son, S. B., Eshraghi, A., Blanke, S., & Bradley, K. (2015) · PLoS ONE · doi:10.1371/journal.pone.0143977 · Cited by 19
Nuclear trafficking of the HIV-1 pre-integration complex depends on the ADAM10 intracellular domain
Endsley, M., Somasunderam, A., Li, G.-Y., Oezguen, N., Thiviyanathan, V., Murray, J., Rubin, D. H., Hodge, T., O'Brien, W., Lewis, B., et al. (2014) · Virology · doi:10.1016/j.virol.2014.02.006 · Cited by 19
“In support, other groups have also implicated ADAM10 as important for HIV-1 replication (Brass et al., 2008; Swaminathan et al., 2012).”
Background
Viruses Challenge Selectivity Barrier of Nuclear Pores
Labokha, A. A., & Fassati, A. (2013) · Viruses · doi:10.3390/v5102410 · Cited by 22
Brain Transcriptome-Wide Screen for HIV-1 Nef Protein Interaction Partners Reveals Various Membrane-Associated Proteins
Kammula, E. C., Mötter, J., Gorgels, A., Jonas, E., Hoffmann, S., & Willbold, D. (2012) · PLoS ONE · doi:10.1371/journal.pone.0051578 · Cited by 19
“Several recent genome-wide screening approaches for reduced viral replication upon RNAi knock down revealed more than 1000 annotated gene candidates for cellular proteins, also called ''HIV-1 dependency factors'' (HDF) that negatively influenced viral replication in cell culture [28] [29] [30] [31] .”
Background
Rapid Analysis of Pharmacology for Infectious Diseases
Hopkins, A., Bickerton, G. R., Carruthers, I. M., Boyer, S., Rubin, H., & Overington, J. P. (2011) · Current Topics in Medicinal Chemistry · doi:10.2174/156802611795429130 · Cited by 19
“In addition to the large-scale knock out studies in pathogen genomes, genome-wide RNA interference is increasingly being used to identify host factors critical for pathogen infection, such as HIV-1 [14], the flavivruses [78], West Nile virus [15] and Dengue virus [79].”
Background
The Cellular Factors Vps18 and Mon2 Are Required for Efficient Production of Infectious HIV-1 Particles
Tomita, Y., Noda, T., Fujii, K., Watanabe, T., Morikawa, Y., & Kawaoka, Y. (2011) · Journal of Virology · doi:10.1128/jvi.00846-10 · Cited by 19
“, Tsg101) that function in the late stage of the viral life cycle were not identified (4, 28, 55).”
Background
Detecting host factors involved in virus infection by observing the clustering of infected cells in siRNA screening images
Suratanee, A., Rebhan, I., Matula, P., Kumar, A., Kaderali, L., Rohr, K., Bartenschlager, R. F. W., Eils, R., & König, R. (2010) · Bioinformatics · doi:10.1093/bioinformatics/btq398 · Cited by 22
Positive selection of HIV host factors and the evolution of lentivirus genes
Bożek, K., & Lengauer, T. (2010) · BMC Evolutionary Biology · doi:10.1186/1471-2148-10-186 · Cited by 17
Tpl2/AP-1 Enhances Murine Gammaherpesvirus 68 Lytic Replication
Li, X., Feng, J., Chen, S.-J., Peng, L., He, W.-Y., Qi, J., Deng, H., & Sun, R. (2009) · Journal of Virology · doi:10.1128/jvi.01856-09 · Cited by 19
“In addition, multiple components of a known cellular pathway or machinery can be identified through such screens, which help to link a specific pathway or machinery to virus replication (3, 10, 34).”
Background
Identification of the P‐TEFb complex‐interacting domain of Brd4 as an inhibitor of HIV‐1 replication by functional cDNA library screening in MT‐4 cells
Urano, E., Kariya, Y., Futahashi, Y., Ichikawa, R., Hamatake, M., Fukazawa, H., Morikawa, Y., Yoshida, T., Koyanagi, Y., Yamamoto, N., et al. (2008) · FEBS Letters · doi:10.1016/j.febslet.2008.10.047 · Cited by 19
SRSF1 acts as an IFN-I-regulated cellular dependency factor decisively affecting HIV-1 post-integration steps
Sertznig, H., Roesmann, F., Wilhelm, A., Heininger, D., Bleekmann, B., Elsner, C., Santiago, M. L., Schuhenn, J., Karakoese, Z., Benatzy, Y., et al. (2022) · Frontiers in Immunology · doi:10.3389/fimmu.2022.935800 · Cited by 22
Complex Relationships between HIV-1 Integrase and Its Cellular Partners
Rozina, A., Anisenko, A. N., Kikhai, T. F., Silkina, M. O., & Gottikh, M. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms232012341 · Cited by 22
The autophagy protein ATG9A promotes HIV-1 infectivity
Mailler, É., Waheed, A. A., Park, S., Gershlick, D. C., Freed, E. O., & Bonifacino, J. S. (2019) · Retrovirology · doi:10.1186/s12977-019-0480-3 · Cited by 19
DDX17 Specifically, and Independently of DDX5, Controls Use of the HIV A4/5 Splice Acceptor Cluster and Is Essential for Efficient Replication of HIV
Sithole, N., Williams, C. A. L., Vaughan, A., Kenyon, J. C., & Lever, A. M. L. (2018) · Journal of Molecular Biology · doi:10.1016/j.jmb.2018.06.052 · Cited by 23
Direct binding to GABARAP family members is essential for HIV-1 Nef plasma membrane localization
Boeske, A., Schwarten, M., Ma, P., Tusche, M., Mötter, J., Möller, C., Neudecker, P., Hoffmann, S., & Willbold, D. (2017) · Scientific Reports · doi:10.1038/s41598-017-06319-4 · Cited by 18
A Genome-Wide siRNA Screen Implicates Spire1/2 in SipA-Driven Salmonella Typhimurium Host Cell Invasion
Andritschke, D., Dilling, S., Emmenlauer, M., Welz, T., Schmich, F., Misselwitz, B., Rämö, P., Rottner, K., Kerkhoff, E., Wada, T., et al. (2016) · PLoS ONE · doi:10.1371/journal.pone.0161965 · Cited by 19
Identification of a gp41 Core-Binding Molecule with Homologous Sequence of Human TNNI3K-Like Protein as a Novel Human Immunodeficiency Virus Type 1 Entry Inhibitor
Zhu, Y., Lu, L., Xu, L.-L., Yang, H.-W., Jiang, S.-B., & Chen, Y.-H. (2010) · Journal of Virology · doi:10.1128/jvi.00644-10 · Cited by 18
“Recently, a number of human proteins that are required for HIV infection have been identified through genomewide screenings (2, 27, 56).”
Background
Cellular Transcription Factor ZASC1 Regulates Murine Leukemia Virus Transcription
Bruce, J. W., Hierl, M. A., Young, J., & Ahlquist, P. (2010) · Journal of Virology · doi:10.1128/jvi.00299-10 · Cited by 18
“Many cellular factors that regulate different steps of retroviral replication were identified previously through a combination of genetic and biochemical approaches (4, 7, 10, 18, 23, 34, 37).”
Background
Illuminating the host – How RNAi screens shed light on host‐pathogen interactions
Prudêncio, M., & Lehmann, M. (2009) · Biotechnology Journal · doi:10.1002/biot.200900071 · Cited by 18
“[58] identified 273 host factors required for HIV-1 replication.”
Background
Human immunodeficiency virus type 1 chronic infection is associated with different gene expression in MT-4, H9 and U937 cell lines.
Olivares, I., Ballester, A., Lombardía, L., Domínguez, O., & López-Galíndez, C. (2009) · Virus Research · doi:10.1016/j.virusres.2008.09.010 · Cited by 18
The HIV-1 Transcriptional Program: From Initiation to Elongation Control
D’Orso, I. (2024) · Journal of Molecular Biology · doi:10.1016/j.jmb.2024.168690 · Cited by 21
The Role of Capsid in the Early Steps of HIV-1 Infection: New Insights into the Core of the Matter
AlBurtamani, N., Paul, A., & Fassati, A. (2021) · Viruses · doi:10.3390/v13061161 · Cited by 23
Host-HIV-1 Interactome: A Quest for Novel Therapeutic Intervention
Shukla, E., & Chauhan, R. (2019) · Cells · doi:10.3390/cells8101155 · Cited by 19
“These include Nup98, Nup85, Nup133, Nup107, Nup160, Nup153, Nup214, Nup358, Nup155 and TNPO3 (tansportin 3) [86,87,100,101].”
Genome-Wide Analyses Reveal Gene Influence on HIV Disease Progression and HIV-1C Acquisition in Southern Africa
Xie, W., Agniel, D. M., Shevchenko, A. K., Malov, S. V., Svitin, A., Cherkasov, N., Baum, M. K., Campa, A. L., Gaseitsiwe, S., Bussmann, H., et al. (2017) · AIDS Research and Human Retroviruses · doi:10.1089/aid.2016.0017 · Cited by 16
Targeting Virus-host Interactions of HIV Replication
Weydert, C., Rijck, J. D., Christ, F., & Debyser, Z. (2015) · Current Topics in Medicinal Chemistry · doi:10.2174/1568026615666150901115106 · Cited by 16
Reviving an old HIV-1 gene: the HIV-1 antisense protein.
Torresilla, C., Mesnard, J., & Barbeau, B. (2015) · Current HIV Research · doi:10.2174/1570162x12666141202125943 · Cited by 17
Applications of RNA interference high-throughput screening technology in cancer biology and virology
Gao, S., Yang, C., Jiang, S., Xu, X.-N., Lu, X., He, Y.-W., Cheung, A., & Wang, H. (2014) · Protein & Cell · doi:10.1007/s13238-014-0076-6 · Cited by 17
“Indeed, many novel genes involved in virus entry and replication in the host have been uncovered (Brass et al., 2008; Brass et al., 2009).”
BackgroundMethodInfluential
Innovation in academic chemical screening: filling the gaps in chemical biology
Hasson, S. A., & Inglese, J. (2013) · Current Opinion in Chemical Biology · doi:10.1016/j.cbpa.2013.04.018 · Cited by 18
Infection systems biology: from reactive to proactive (P4) medicine.
Bengoechea, J. (2012) · International Microbiology · doi:10.2436/20.1501.01.158 · Cited by 17
HIV‐1, ubiquitin and ubiquitin‐like proteins: the dialectic interactions of a virus with a sophisticated network of post‐translational modifications
Biard‐Piechaczyk, M., Borel, S., Espert, L., de Bettignies, G., & Coux, O. (2011) · Biology of the Cell · doi:10.1111/boc.201100112 · Cited by 18
Systems biology of tuberculosis
Chandra, N. R., Kumar, D., & Rao, K. V. S. (2011) · Tuberculosis · doi:10.1016/j.tube.2011.02.008 · Cited by 25
Alpha‐1 antitrypsin variants in plasma from HIV‐infected patients revealed by proteomic and glycoproteomic analysis
Zhang, L.-J., Jia, X.-F., Zhang, X., Cao, J., Yang, P.-Y., Qiu, C., Shen, Y., Ma, F., Liu, L., Sun, J.-J., et al. (2010) · Electrophoresis · doi:10.1002/elps.201000153 · Cited by 17
The next frontier of systems biology: higher-order and interspecies interactions
Fischbach, M. A., & Krogan, N. J. (2010) · Genome biology · doi:10.1186/gb-2010-11-5-208 · Cited by 15
Functional association between influenza A (H1N1) virus and human.
Huang, T., Cui, W., He, Z.-S., Hu, L., Liu, F., Wen, T., Li, Y.-X., & Cai, Y. (2009) · Biochemical and Biophysical Research Communications - BBRC · doi:10.1016/j.bbrc.2009.08.131 · Cited by 17
RNA‐Interferenz: von den Grundlagen zur therapeutischen Anwendung
Kurreck, J. (2009) · Angewandte Chemie · doi:10.1002/ange.200802092 · Cited by 26
Signal-regulatory protein alpha is an anti-viral entry factor targeting viruses using endocytic pathways
Sarute, N., Cheng, H., Yan, Z., Salas-Briceno, K., Richner, J. M., Rong, L., & Ross, S. R. (2021) · PLoS Pathogens · doi:10.1371/journal.ppat.1009662 · Cited by 21
Molecular chaperone function of stress inducible Hsp70 is critical for intracellular multiplication of Toxoplasma gondii
Mitra, P., Deshmukh, A. S., & Choudhury, C. (2020) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2020.118898 · Cited by 16
Integrated transcriptomic analysis on small yellow follicles reveals that sosondowah ankyrin repeat domain family member A inhibits chicken follicle selection
Zhong, C., Liu, Z.-M., Qiao, X.-B., Kang, L., Sun, Y., & Jiang, Y.-L. (2020) · Asian-Australasian Journal of Animal Sciences · doi:10.5713/ajas.20.0404 · Cited by 16
“In HeLa-derived TZM-bl cells, knockdown of SOWAHA by siRNA inhibits HIV-1 replication [13].”
Background
The Genome-wide Methylation Profile of CD4+ T Cells From Individuals With Human Immunodeficiency Virus (HIV) Identifies Distinct Patterns Associated With Disease Progression
Morón‐López, S., Urrea, V., Dalmau, J., López, M., Puertas, M. C., Ouchi, D., Gómez, A., Passaes, C., Mothe, B., Brander, C., et al. (2020) · Clinical Infectious Diseases · doi:10.1093/cid/ciaa1047 · Cited by 19
Identification of novel targets for host-directed therapeutics against intracellular Staphylococcus aureus
Bravo-Santano, N., Capilla-Lasheras, P., Mateos, L. M., Calle, Y., Behrends, V., & Letek, M. (2019) · Scientific Reports · doi:10.1038/s41598-019-51894-3 · Cited by 16
Y-box-binding protein 1 supports the early and late steps of HIV replication
Weydert, C., van Heertum, B., Dirix, L., Houwer, S. D., Wit, F. D., Mast, J., Husson, S. J., Busschots, K., König, R., Gijsbers, R., et al. (2018) · PLoS ONE · doi:10.1371/journal.pone.0200080 · Cited by 18
eIF4A2 is a host factor required for efficient HIV-1 replication
Ndzinu, J., Takeuchi, H., Saito, H., Yoshida, T., & Yamaoka, S. (2018) · Microbes and Infection · doi:10.1016/j.micinf.2018.05.001 · Cited by 15
Control of yeast retrotransposons mediated through nucleoporin evolution
Rowley, P. A., Patterson, K., Sandmeyer, S. B., & Sawyer, S. L. (2018) · PLoS Genetics · doi:10.1371/journal.pgen.1007325 · Cited by 17
Cytosolic sulfotransferase 1A1 regulates HIV-1 minus-strand DNA elongation in primary human monocyte-derived macrophages
Swann, J., Murry, J. P., & Young, J. A. T. (2016) · Virology Journal · doi:10.1186/s12985-016-0491-9 · Cited by 14
A Novel Class of HIV-1 Antiviral Agents Targeting HIV via a SUMOylation-Dependent Mechanism
Madu, I. G., Li, S., Li, B.-Z., Li, H.-T., Chang, T., Li, Y.-J., Vega, R., Rossi, J., Yee, J.-K., Zaia, J., et al. (2015) · Scientific Reports · doi:10.1038/srep17808 · Cited by 16
“This finding is consistent with recent whole-genome screens, in which proteins involved in SUMOylation were identified as host factors that affect HIV-1 replication, including SENP5 25,26 .”
A parallel genome-wide RNAi screening strategy to identify host proteins important for entry of Marburg virus and H5N1 influenza virus
Cheng, H., Koning, K., O’Hearn, A. E., Wang, M.-X., Rumschlag-Booms, E., Varhegyi, E., & Rong, L.-J. (2015) · Virology Journal · doi:10.1186/s12985-015-0420-3 · Cited by 16
Target silencing of components of the Conserved Oligomeric Golgi complex impairs HIV-1 replication
Liu, S., Dominska-Ngowe, M., & Dykxhoorn, D. (2014) · Virus Research · doi:10.1016/j.virusres.2014.08.015 · Cited by 16
“…to components of the GARP/VFT complex, members of an additional multiprotein tethering factor, the conserved oligomeric golgi (COG) complex, were identified in a RNAi-based functional genomic screen to identify host factors whose silencing impairs the replication of HIV-1 (Brass et al., 2008).”
BackgroundResultInfluentialSelf-citation
Potential directions for chicken immunology research.
Stewart, C., Keyburn, A., Deffrasnes, C., & Tompkins, S. (2013) · Developmental and Comparative Immunology · doi:10.1016/j.dci.2013.05.011 · Cited by 16
Influence of lipoplex surface charge on siRNA delivery: application to the in vitro downregulation of CXCR4 HIV-1 co-receptor
Lavigne, C., Slater, K., Gajanayaka, N., Duguay, C., Peyrotte, E. A., Fortier, G., Simard, M., Kell, A., Barnes, M. L., & Thierry, A. (2013) · Expert Opinion on Biological Therapy · doi:10.1517/14712598.2013.743526 · Cited by 16
“In two recent genomic studies, 15 host proteins have been identified to be essential for a complete round of viral replication [1,2].”
Background
Closing the door to human immunodeficiency virus
Kang, Y., Guo, J., & Chen, Z. (2013) · Protein & Cell · doi:10.1007/s13238-012-2111-9 · Cited by 15
“Through a genome-wide screen by siRNA library, some novel targets in protein transport machinery have been identified to be essential for HIV-1 entry (Brass et al., 2008).”
Background
A Whole-Genome RNA Interference Screen for Human Cell Factors Affecting Myxoma Virus Replication
Teferi, W. M., Dodd, K., Maranchuk, R. A., Favis, N. A., & Evans, D. H. (2013) · Journal of Virology · doi:10.1128/jvi.02617-12 · Cited by 17
The GEF1 Proton-Chloride Exchanger Affects Tombusvirus Replication via Regulation of Copper Metabolism in Yeast
Sasvári, Z., Kovalev, N., & Nagy, P. D. (2012) · Journal of Virology · doi:10.1128/jvi.02003-12 · Cited by 16
Evaluation of the interactions of HIV-1 integrase with small ubiquitin-like modifiers and their conjugation enzyme Ubc9.
Li, Z.-H., Wu, S.-W., Wang, J.-J., Li, W.-J., Lin, Y.-D., Ji, C., Xue, J.-L., & Chen, J.-Z. (2012) · International Journal of Molecular Medicine · doi:10.3892/ijmm.2012.1088 · Cited by 16
Microfluidic platforms for RNA interference screening of virus-host interactions.
Schudel, B. R., Harmon, B., Abhyankar, V., Pruitt, B. W., Negrete, O. A., & Singh, A. K. (2012) · Lab on a Chip · doi:10.1039/c2lc41165b · Cited by 16
In Vivo Selection of CD4+ T Cells Transduced with a Gamma-Retroviral Vector Expressing a Single-Chain Intrabody Targeting HIV-1 Tat
Braun, S. E., Taube, R., Zhu, Q., Wong, F. E., Murakami, A., Kamau, E., Dwyer, M., Qiu, G., Daigle, J., Carville, A., et al. (2012) · Human Gene Therapy · doi:10.1089/hum.2011.184 · Cited by 16
“…and Sui, 2007; Rossi et al. , 2007; Kumar et al. , 2008; Lo et al. , 2008) have resulted in efﬁcient and speciﬁc reduction of HIV replication in vitro (Brass et al. , 2008; Ko¨nig et al. , 2008; Nathans et al. , 2008) and, in some cases, protection and selection of the transduced cells (Klebba et…”
Deep Sequencing Analyses of DsiRNAs Reveal the Influence of 3′ Terminal Overhangs on Dicing Polarity, Strand Selectivity, and RNA Editing of siRNAs
Zhou, J.-H., Song, M.-S., Jacobi, A. M., Behlke, M., Wu, X.-W., & Rossi, J. (2012) · Molecular Therapy: Nucleic Acids · doi:10.1038/mtna.2012.6 · Cited by 16
RNA Interference as a Tool for Exploring HIV-1 Robustness
Nevot, M., Martrus, G., Clotet, B., & Martı́nez, M. Á. (2011) · Journal of Molecular Biology · doi:10.1016/j.jmb.2011.08.035 · Cited by 15
Host sequence motifs shared by HIV predict response to antiretroviral therapy
Dampier, W., Evans, P., Ungar, L., & Tozeren, A. (2009) · BMC Medical Genomics · doi:10.1186/1755-8794-2-47 · Cited by 16
Cyclophilin function in Cancer; lessons from virus replication
Lavin, P. T., & Gee, M. M. M. (2015) · Current Molecular Pharmacology · doi:10.2174/1874467208666150519115443 · Cited by 18
“Furthermore, Rab6 and Vps53 implicate the retrograde pathway in viral entry [165].”
Background
Aneuploidy-induced proteostasis disruption impairs mitochondrial functions and mediates aggregation of mitochondrial precursor proteins through SQSTM1/p62
Amponsah, P. S., Boekenkamp, J.-E., Kurpa, O., Lenhard, S., Myronova, A., Velazquez, D. O. V., Hirschelmann, C., Behrends, C., Herrmann, J. M., Räschle, M., et al. (2025) · Nature Communications · doi:10.1038/s41467-025-60857-4 · Cited by 15
HIV-1-induced nuclear invaginations mediated by VAP-A, ORP3, and Rab7 complex explain infection of activated T cells
Santos, M. F., Rappa, G., Karbanová, J., Diana, P., Cirrincione, G., Carbone, D., Manna, D., Aalam, F., Wang, D., Vanier, C. H., et al. (2023) · Nature Communications · doi:10.1038/s41467-023-40227-8 · Cited by 17
Nucleoporin 85 interacts with influenza A virus PB1 and PB2 to promote its replication by facilitating nuclear import of ribonucleoprotein
Ling, Y., Wang, H., Han, M.-Q., Wang, D., Hu, Y., Zhou, K., & Li, Y. (2022) · Frontiers in Microbiology · doi:10.3389/fmicb.2022.895779 · Cited by 15
The HIV-1 Capsid: From Structural Component to Key Factor for Host Nuclear Invasion
Scoca, V., & Nunzio, F. D. (2021) · Viruses · doi:10.3390/v13020273 · Cited by 26
Network-Based Analysis of OMICs Data to Understand the HIV–Host Interaction
Ivanov, S. M., Lagunin, A. A., Filimonov, D. A., & Tarasova, O. A. (2020) · Frontiers in Microbiology · doi:10.3389/fmicb.2020.01314 · Cited by 24
Interleukin-27 Post-Translationally Regulates Y-Box Binding Protein-1 to Inhibit HIV-1 Replication in Human CD4+ T Cells
Poudyal, D., Yang, J., Chen, Q., Goswami, S., Adelsberger, J., Das, S., Herman, A., Hornung, R., Andresson, T., & Imamichi, T. (2019) · AIDS (London) · doi:10.1097/qad.0000000000002288 · Cited by 15
Characterisation and function of TRIM23 in grass carp (Ctenopharyngodon idella)
Chu, P.-F., He, L.-B., Yang, C., Li, Y.-Y., Huang, R., Liao, L.-J., Li, Y.-M., Zhu, Z.-Y., & Wang, Y.-P. (2019) · Fish and Shellfish Immunology · doi:10.1016/j.fsi.2019.03.025 · Cited by 15
Genome-wide screens in accelerated human stem cell-derived neural progenitor cells identify Zika virus host factors and drivers of proliferationPreprint
Wells, M. F., Salick, M. R., Piccioni, F., Guss, E. J., Mitchell, J. M., Worringer, K. A., Raymond, J. J., Kommineni, S., Chan, K., Ho, D. J., et al. (2018) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/476440 · Cited by 15
N-terminally truncated POM121C inhibits HIV-1 replication
Saito, H., Takeuchi, H., Masuda, T., Noda, T., & Yamaoka, S. (2017) · PLoS ONE · doi:10.1371/journal.pone.0182434 · Cited by 16
The tumour suppressor APC promotes HIV-1 assembly via interaction with Gag precursor protein
Miyakawa, K., Nishi, M., Matsunaga, S., Okayama, A., Anraku, M., Kudoh, A., Hirano, H., Kimura, H., Morikawa, Y., Yamamoto, N., et al. (2017) · Nature Communications · doi:10.1038/ncomms14259 · Cited by 14
Annotating Protein Functional Residues by Coupling High-Throughput Fitness Profile and Homologous-Structure Analysis
Du, Y.-S., Wu, N. C., Jiang, L., Zhang, T.-H., Gong, D., Shu, S., Wu, T.-T., & Sun, R. (2016) · mBio · doi:10.1128/mbio.01801-16 · Cited by 15
“Moreover, vRdRp often recruits cellular machinery for replication and plays a role in inhibition of the cellular immune response (25) (26) (27) (28) (29) (30) (31) .”
Background
Dynein Regulators Are Important for Ecotropic Murine Leukemia Virus Infection
Valle-Tenney, R., Opazo, T., Cancino, J., Goff, S. P., & Arriagada, G. (2016) · Journal of Virology · doi:10.1128/jvi.00863-16 · Cited by 18
Proteomics in the investigation of HIV‐1 interactions with host proteins
Li, M. (2014) · PROTEOMICS - CLINICAL APPLICATIONS · doi:10.1002/prca.201400101 · Cited by 16
Alterations in the nuclear proteome of HIV-1 infected T-cells
DeBoer, J., Jagadish, T., Haverland, N. A., Madson, C. J., Ciborowski, P. S., & Belshan, M. (2014) · Virology · doi:10.1016/j.virol.2014.08.029 · Cited by 16
HIV-1-associated PKA acts as a cofactor for genome reverse transcription
Giroud, C., Chazal, N., Gay, B., Eldin, P., Brun, S., & Briant, L. (2013) · Retrovirology · doi:10.1186/1742-4690-10-157 · Cited by 12
Reconstruction of Cellular Signal Transduction Networks Using Perturbation Assays and Linear Programming
Knapp, B., & Kaderali, L. (2013) · PLoS ONE · doi:10.1371/journal.pone.0069220 · Cited by 15
Interaction of Transportin-SR2 with Ras-related Nuclear Protein (Ran) GTPase
Taltynov, O., Demeulemeester, J., Christ, F., Houwer, S. D., Tsirkone, V. G., Gérard, M., Weeks, S. D., Strelkov, S. V., & Debyser, Z. (2013) · Journal of Biological Chemistry · doi:10.1074/jbc.m113.484345 · Cited by 13
High-throughput RNA interference screens integrative analysis: Towards a comprehensive understanding of the virus-host interplay
Amberkar, S. (2013) · World Journal of Virology · doi:10.5501/wjv.v2.i2.18 · Cited by 14

Authors: Brass, A. L., Dykxhoorn, D. M., Benita, Y., Yan, N., Engelman, A., Xavier, R. J., Lieberman, J., & Elledge, S. J.
Affiliations: Brigham and Women's Hospital (US)
ORCID: Dykxhoorn, Derek M.
Benita, Yair
Yan, Nan
Engelman, Alan
Xavier, Ramnik J.
Lieberman, Judy
Elledge, Stephen J.
Venue: Science
Publisher: American Association for the Advancement of Science (AAAS)
Published: 2008-02-15
Volume: 319
Issue: 5865
Pages: 921-926
ISSN: 0036-8075 (ISSN-L)
1095-9203
DOI: 10.1126/science.1152725
OpenAlex: W2145848202
Cited by: 1,449
References: 0
Citations by year: 2026: 12 · 2025: 18 · 2024: 26 · 2023: 28 · 2022: 38 · 2021: 38 · 2020: 52 · 2019: 59 · 2018: 71 · 2017: 53 · 2016: 62 · 2015: 96 · 2014: 90 · 2013: 150 · 2012: 148
FWCI: 57.05
Type: journal article
Language: en
Open access: closed
Locations: Science (publishedVersion)
PubMed (publishedVersion)
Funders: Howard Hughes Medical Institute
National Institute of Allergy and Infectious Diseases (U19AI056900, AI052014, AI 062773, R01AI062773, R01AI052014, P30AI060354)
National Institute of Diabetes and Digestive and Kidney Diseases (P30DK040561)
Topics: Life Sciences › Immunology and Microbiology › Virology › HIV Research and Treatment
Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
Health Sciences › Medicine › Infectious Diseases › HIV/AIDS drug development and treatment
Keywords: HIV-1, host factors, host-virus interaction, Rab6, viral entry, karyopherins, TNPO3, viral integration, Mediator complex, MED28, viral transcription, antiviral drug targets
MeSH terms: Cytoskeletal Proteins, Humans, Proteins, Transcription, Genetic, Virus Replication, HIV-1, HIV Infections, Virus Integration, Computational Biology, rab GTP-Binding Proteins, Genomics, Karyopherins, Vesicular Transport Proteins, RNA Interference, RNA, Small Interfering, Cell Line, Tumor, Intracellular Signaling Peptides and Proteins, Virus Internalization, Human Immunodeficiency Virus Proteins, Host-Pathogen Interactions, Mediator Complex

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Identification of Host Proteins Required for HIV Infection Through a Functional Genomic Screen

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Metadata

Record

About these checks