Cited by 488RNA Interference and Gene DeliveryRNA Research and SplicingPlant Molecular Biology Research

Life of RISC: Formation, action, and degradation of RNA-induced silencing complex

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Iwakawa, Hiro-oki, Tomari, Yukihide·2022·Molecular Cell

Abstract

Small RNAs regulate a wide variety of biological processes by repressing the expression of target genes at the transcriptional and post-transcriptional levels. To achieve these functions, small RNAs form RNA-induced silencing complex (RISC) together with a member of the Argonaute (AGO) protein family. RISC is directed by its bound small RNA to target complementary RNAs and represses their expression through mRNA cleavage, degradation, and/or translational repression. Many different factors fine-tune RISC activity and stability-from guide-target RNA complementarity to the recruitment of other protein partners to post-translational modifications of RISC itself. Here, we review recent progress in understanding RISC formation, action, and degradation, and discuss new, intriguing questions in the field.

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Crossref · 10.1016/j.molcel.2021.11.026

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Authors
Iwakawa, H.-O., & Tomari, Y.
Affiliations
Institute for Quantitative Biosciences, The University of Tokyo (JP)
Corresponding author
  • Iwakawa, Hiro-oki
  • Tomari, Yukihide
Venue
Molecular Cell
Publisher
Elsevier BV
Published
2022-01
Volume
82
Issue
1
Pages
30-43
ISSN
1097-2765 (ISSN-L)
OpenAlex
W4200304551
Cited by
488
References
190
Citations by year
2026: 97 · 2025: 151 · 2024: 115 · 2023: 91 · 2022: 34
FWCI
6.34
Type
journal article
Language
en
Open access
Locations
  • Molecular Cell (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Japan Society for the Promotion of Science (18H05271)
  • Japan Science and Technology Agency
  • Precursory Research for Embryonic Science and Technology (JPMJPR18K2)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
Keywords
RNA-induced silencing complex, Argonaute proteins, small RNA, RNA interference, post-transcriptional gene silencing, mRNA degradation, protein synthesis inhibition, post-translational modification
MeSH terms
Animals, Humans, Protein Binding, RNA, RNA, Messenger, RNA Stability, RNA, Small Interfering, RNA-Induced Silencing Complex, MicroRNAs, Protein Stability, Proteolysis, Argonaute Proteins

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