RNAi in Budding Yeast
Open accessAbstract
Yeast Joins RNAi Club: RNA interference (RNAi) silences gene expression via small interfering (si) RNAs that bind to target sequences. RNAi has been found in almost all eukaryotes examined, with the notable exception of the budding yeast, Saccharomyces cerevisiae , one of the most prominent organisms used in the study of molecular biology. Indeed, RNAi has been thought to be missing from all budding yeast. Drinnenberg et al. (p. 544 , published online 10 September; see the Perspective by Moazed ) now show that several species of budding yeast do possess RNAi, which seems to act primarily on transposable elements and subtelomeric repeats. A noncanonical Dicer protein generates siRNAs that are loaded onto Ago1 protein in S. castellii . Introduction of these two genes into S. cerevisiae was sufficient to reconstitute RNAi, where it also acted to dampen the activity of transposons.
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C. albicans and other budding yeast species possess noncanonical RNAi proteins.
“It is noteworthy that the canonical RNAi pathway has been lost in C. albicans and other budding yeast species; however, these organisms possess noncanonical RNAi proteins104.”
From Molecular mechanisms governing antifungal drug resistance (2023) · cites this paper as [104]
The canonical RNAi pathway has been lost in C. albicans and other budding yeast species.
“It is noteworthy that the canonical RNAi pathway has been lost in C. albicans and other budding yeast species; however, these organisms possess noncanonical RNAi proteins104.”
From Molecular mechanisms governing antifungal drug resistance (2023) · cites this paper as [104]
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Molecular mechanisms governing antifungal drug resistance
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- Authors
- Drinnenberg, I. A., Weinberg, D. E., Xie, K. T., Mower, J. P., Wolfe, K. H., Fink, G. R., & Bartel, D. P.
- Affiliations
- Howard Hughes Medical Institute (US)
- Trinity College Dublin (IE)
- Whitehead Institute for Biomedical Research (US)
- Corresponding author
- Drinnenberg, Ines A.
- Weinberg, David E.
- Xie, Kathleen T.
- Mower, Jeffrey P.
- Bartel, David P.
- ORCID
- Venue
- Science
- Published
- 2009-10-23
- Volume
- 326
- Issue
- 5952
- Pages
- 544-550
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- PMC
- PMC3786161
- OpenAlex
- W1499220571
- Cited by
- 524
- References
- 38
- Citations by year
- 2026: 20 · 2025: 26 · 2024: 20 · 2023: 22 · 2022: 29 · 2021: 32 · 2020: 31 · 2019: 28 · 2018: 24 · 2017: 29 · 2016: 43 · 2015: 33 · 2014: 34 · 2013: 41 · 2012: 32
- FWCI
- 15.02
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- DSpace@MIT (Massachusetts Institute of Technology) (submittedVersion, cc-by-nc-sa)
- MPG.PuRe (Max Planck Society) (submittedVersion)
- Funders
- National Science Foundation
- Howard Hughes Medical Institute
- Irish Research Council for Science, Engineering and Technology
- Science Foundation Ireland
- National Institutes of Health
- National Institute of General Medical Sciences (GM067031, R01GM067031, R01GM040266, GM0305010, GM040266)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Chromosomal and Genetic Variations
- Keywords
- RNA interference, Saccharomyces cerevisiae, Candida albicans, siRNA, gene silencing, transposable elements, endogenous retrotransposons, Argonaute proteins
- MeSH terms
- Saccharomycetales, Fungal Proteins, Genes, Fungal, Mutation, Repetitive Sequences, Nucleic Acid, RNA, Double-Stranded, RNA, Fungal, RNA, Messenger, Saccharomyces, Saccharomyces cerevisiae, Transcription, Genetic, Transformation, Genetic, Open Reading Frames, Sequence Analysis, RNA, Retroelements, Gene Expression Profiling, Saccharomyces cerevisiae Proteins, RNA Interference, RNA, Small Interfering, Ribonuclease III, Genetic Loci
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