Genetic screens in human cells using the CRISPR-Cas9 system

Cited by (142)

Items 101–142 of 142

1. Genetic interaction mapping and exon-resolution functional genomics with a hybrid Cas9–Cas12a platform
   Gonatopoulos-Pournatzis, Thomas, Aregger, Michael, Brown, Kevin R., Farhangmehr, Shaghayegh, Braunschweig, Ulrich, Ward, Henry N., Ha, Kevin C. H., Weiss, Alexander, Billmann, Maximilian, Durbic, Tanja et al. · 2020 · Nature Biotechnology
2. A new class of temporarily phenotypic enhancers identified by CRISPR/Cas9-mediated genetic screening
   Diao, Yarui, Li, Bin, Meng, Zhipeng, Jung, Inkyung, Lee, Ah Young, Dixon, Jesse, Maliskova, Lenka, Guan, Kun-liang, Shen, Yin, Ren, Bing · 2016 · Genome Research
3. Imaging-based pooled CRISPR screening reveals regulators of lncRNA localization
   Wang, Chong, Lu, Tian, Emanuel, George, Babcock, Hazen P., Zhuang, Xiaowei · 2019 · Proceedings of the National Academy of Sciences
4. Molecular biology at the cutting edge: A review on CRISPR/CAS9 gene editing for undergraduates
   Thurtle‐Schmidt, Deborah M., Lo, Te‐Wen · 2018 · Biochemistry and Molecular Biology Education
5. Genome-wide CRISPR screen for PARKIN regulators reveals transcriptional repression as a determinant of mitophagy
   Potting, Christoph, Crochemore, Christophe, Moretti, Francesca, Nigsch, Florian, Schmidt, Isabel, Manneville, Carole, Carbone, Walter, Knehr, Judith, DeJesus, Rowena, Lindeman, Alicia et al. · 2018 · Proceedings of the National Academy of Sciences
6. Long-term expansion and differentiation of adult murine epidermal stem cells in 3D organoid cultures
   Boonekamp, Kim E., Kretzschmar, Kai, Wiener, Dominique J., Asra, Priyanca, Derakhshan, Sepideh, Puschhof, Jens, López-Iglesias, Carmen, Peters, Peter J., Basak, Onur, Clevers, Hans · 2019 · Proceedings of the National Academy of Sciences
7. Model-based understanding of single-cell CRISPR screening
   Duan, Bin, Zhou, Chi, Zhu, Chengyu, Yu, Yifei, Li, Gaoyang, Zhang, Shihua, Zhang, Chao, Ye, Xiangyun, Ma, Hanhui, Qu, Shen et al. · 2019 · Nature Communications
8. A simple and efficient CRISPR/Cas9 platform for induction of single and multiple, heritable mutations in barley (Hordeum vulgare L.)
   Gasparis, Sebastian, Kała, Maciej, Przyborowski, Mateusz, Łyżnik, Leszek A., Orczyk, Wacław, Nadolska-Orczyk, Anna · 2018 · Plant Methods
9. Synthetic Lethal Vulnerabilities in <i>KRAS</i>-Mutant Cancers
   Aguirre, Andrew J., Hahn, William C. · 2018 · Cold Spring Harbor Perspectives in Medicine
10. CHO-omics review: the impact of current and emerging technologies on Chinese hamster ovary based bioproduction
    Stolfa, Gino, Smonskey, Matthew T., Boniface, Ryan, Hachmann, Anna‐Barbara, Gulde, Paul, Joshi, Atul D., Pierce, Anson P., Jacobia, Scott J., Campbell, Andrew · 2018 · Biotechnology Journal
11. BIN1 regulates BACE1 intracellular trafficking and amyloid-β production
    Miyagawa, Toji, Ebinuma, Ihori, Morohashi, Yuichi, Hori, Yukiko, Young Chang, Mee, Hattori, Haruhiko, Maehara, Tomoaki, Yokoshima, Satoshi, Fukuyama, Tohru, Tsuji, Shoji et al. · 2016 · Human Molecular Genetics
12. scMAGeCK links genotypes with multiple phenotypes in single-cell CRISPR screens
    Yang, Lin, Zhu, Yuqing, Yu, Hua, Cheng, Xiaolong, Chen, Sitong, Chu, Yulan, Huang, He, Zhang, Jin, Li, Wei · 2020 · Genome Biology
13. Focal Adhesion Kinase Regulates the DNA Damage Response and Its Inhibition Radiosensitizes Mutant <i>KRAS</i> Lung Cancer
    Tang, Ke-Jing, Constanzo, Jerfiz D., Venkateswaran, Niranjan, Melegari, Margherita, Ilcheva, Mariya, Morales, Julio C., Skoulidis, Ferdinandos, Heymach, John V., Boothman, David A., Scaglioni, Pier Paolo · 2016 · Clinical Cancer Research
14. Chromatin-Bound Oxidized α-Synuclein Causes Strand Breaks in Neuronal Genomes in in vitro Models of Parkinson’s Disease
    Vasquez, Velmarini, Mitra, Joy, Hegde, Pavana M., Pandey, Arvind, Sengupta, Shiladitya, Mitra, Sankar, Rao, K.S., Hegde, Muralidhar L. · 2017 · Journal of Alzheimer's Disease
15. A Landscape of Therapeutic Cooperativity in KRAS Mutant Cancers Reveals Principles for Controlling Tumor Evolution
    Anderson, Grace R., Winter, Peter S., Lin, Kevin H., Nussbaum, Daniel P., Cakir, Merve, Stein, Elizabeth M., Soderquist, Ryan S., Crawford, Lorin, Leeds, Jim C., Newcomb, Rachel et al. · 2017 · Cell Reports
16. Data Storage Based on DNA
    Hao, Yaya, Li, Qian, Fan, Chunhai, Wang, Fei · 2021 · Small Structures
17. A genome-scale CRISPR-Cas9 screening in myeloma cells identifies regulators of immunomodulatory drug sensitivity
    Liu, Jiye, Song, Tianyu, Zhou, Wenrong, Xing, Lijie, Wang, Su, Ho, Matthew, Peng, Zhengang, Tai, Yu-Tzu, Hideshima, Teru, Anderson, Kenneth C. et al. · 2019 · Leukemia
18. Guide Swap enables genome-scale pooled CRISPR-Cas9 screening in human primary cells
    Ting, Pamela Y., Parker, Albert E., Lee, J. Scott, Trussell, Chris, Sharif, Orzala, Luna, Fabio, Federe, Glenn, Barnes, S. Whitney, Walker, John R., Vance, Julie et al. · 2018 · Nature Methods
19. Large-scale image-based profiling of single-cell phenotypes in arrayed CRISPR-Cas9 gene perturbation screens
    de Groot, Reinoud, Lüthi, Joel, Lindsay, Helen, Holtackers, René, Pelkmans, Lucas · 2018 · Molecular Systems Biology
20. De novo identification of essential protein domains from CRISPR-Cas9 tiling-sgRNA knockout screens
    He, Wei, Zhang, Liang, Villarreal, Oscar D., Fu, Rongjie, Bedford, Ella, Dou, Jingzhuang, Patel, Anish Y., Bedford, Mark T., Shi, Xiaobing, Chen, Taiping et al. · 2019 · Nature Communications
21. Genome Editing in iPSC-Based Neural Systems: From Disease Models to Future Therapeutic Strategies
    McTague, Amy, Rossignoli, Giada, Ferrini, Arianna, Barral, Serena, Kurian, Manju A. · 2021 · Frontiers in Genome Editing
22. Multiplexed, image-based pooled screens in primary cells and tissues with PerturbView
    Kudo, Takamasa, Meireles, Ana M., Moncada, Reuben, Chen, Yushu, Wu, Ping, Gould, Joshua, Hu, Xiaoyu, Kornfeld, Opher, Jesudason, Rajiv, Foo, Conrad et al. · 2025 · Nature Biotechnology
23. Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning
    Lin, Shih-Ching, Haga, Kei, Zeng, Xi-Lei, Estes, Mary K. · 2022 · Nature Protocols
24. CRISPR/Cas9 gene editing and natural variation analysis demonstrate the potential for <i>HvARE1</i> in improvement of nitrogen use efficiency in barley
    Karunarathne, Sakura D., Han, Yong, Zhang, Xiao‐Qi, Li, Chengdao · 2022 · Journal of Integrative Plant Biology
25. Pooled genetic perturbation screens with image-based phenotypes
    Feldman, David, Funk, Luke, Le, Anna, Carlson, Rebecca J., Leiken, Michael D., Tsai, FuNien, Soong, Brian, Singh, Avtar, Blainey, Paul C. · 2022 · Nature Protocols
26. Lentiviral co-packaging mitigates the effects of intermolecular recombination and multiple integrations in pooled genetic screens
    Feldman, David, Singh, Avtar, Garrity, Anthony J., Blainey, Paul C. · 2018
27. Genome engineering of stem cell organoids for disease modeling
    Sun, Yingmin, Ding, Qiurong · 2017 · Protein &amp; Cell
28. WDR41 supports lysosomal response to changes in amino acid availability
    Amick, Joseph, Tharkeshwar, Arun Kumar, Amaya,, Catherine, Ferguson, Shawn M. · 2018 · Molecular Biology of the Cell
29. Simple and Efficient Targeted Intracellular Protein Delivery with Self‐Assembled Nanovehicles for Effective Cancer Therapy
    He, Xiao, Long, Qipeng, Zeng, Zhiying, Yang, Lan, Tang, Yaqin, Feng, Xuli · 2019 · Advanced Functional Materials
30. Genome-wide CRISPR screens reveal genetic mediators of cereblon modulator toxicity in primary effusion lymphoma
    Patil, Ajinkya, Manzano, Mark, Gottwein, Eva · 2019 · Blood Advances
31. Genome-wide synthetic lethal CRISPR screen identifies FIS1 as a genetic interactor of ALS-linked C9ORF72
    Chai, Noori, Haney, Michael S., Couthouis, Julien, Morgens, David W., Benjamin, Alyssa, Wu, Kathryn, Ousey, James, Fang, Shirleen, Finer, Sarah, Bassik, Michael C. et al. · 2020 · Brain Research
32. Machine learning-based prediction models to guide the selection of Cas9 variants for efficient gene editing
    Li, Jianbo, Wu, Panfeng, Cao, Zhoutao, Huang, Guanlan, Lu, Zhike, Yan, Jianfeng, Zhang, Heng, Zhou, Yangfan, Liu, Rong, Chen, Hui et al. · 2024 · Cell Reports
33. CRISP-view: a database of functional genetic screens spanning multiple phenotypes
    Cui, Yingbo, Cheng, Xiaolong, Chen, Qing, Song, Bicna, Chiu, Anthony, Gao, Yuan, Dawson, Tyson, Chao, Lumen, Zhang, Wubing, Li, Dian et al. · 2021 · Nucleic Acids Research
34. Scribble sub-cellular localization modulates recruitment of YES1 to regulate YAP1 phosphorylation
    Zhao, Dongyu, Yin, Zhangyuan, Soellner, Matthew B., Martin, Brent R. · 2021 · Cell Chemical Biology
35. A genome‐scale CRISPR knock‐out screen in chronic myeloid leukemia identifies novel drug resistance mechanisms along with intrinsic apoptosis and MAPK signaling
    Lewis, Matthieu, Prouzet‐Mauléon, Valérie, Lichou, Florence, Richard, Elodie, Iggo, Richard, Turcq, Béatrice, Mahon, François‐Xavier · 2020 · Cancer Medicine
36. Learning to quantify uncertainty in off-target activity for CRISPR guide RNAs
    Özden, Furkan, Minary, Peter · 2024 · Nucleic Acids Research
37. Dissecting mammalian immunity through mutation
    Siggs, Owen M · 2014 · Immunology &amp; Cell Biology
38. BoostMEC: predicting CRISPR-Cas9 cleavage efficiency through boosting models
    Zarate, Oscar A., Yang, Yiben, Wang, Xiaozhong, Wang, Ji-Ping · 2022 · BMC Bioinformatics
39. Hybrid multitask learning reveals sequence features driving specificity in the CRISPR/Cas9 system
    Vora, Dhvani Sandip, Yadav, Shashank, Sundar, Durai · 2023 · Biomolecules
40. Key sequence features of CRISPR RNA for dual-guide CRISPR-Cas9 ribonucleoprotein complexes assembled with wild-type or HiFi Cas9
    Okada, Keita, Aoki, Kanae, Tabei, Teruyuki, Sugio, Kota, Imai, Katsunori, Bonkohara, Yuki, Kamachi, Yusuke · 2022 · Nucleic Acids Research
41. Atypical cellular responses mediated by intracellular constitutive active TrkB (NTRK2) kinase domains and a solely intracellular NTRK2-fusion oncogene
    Gupta, Rohini, Dittmeier, Melanie, Wohlleben, Gisela, Nickl, Vera, Bischler, Thorsten, Luzak, Vanessa, Wegat, Vanessa, Doll, Dennis, Sodmann, Annemarie, Bady, Elena et al. · 2024 · Cancer Gene Therapy
42. Endogenous tagging using split mNeonGreen in human iPSCs for live imaging studies
    Husser, Mathieu C, Pham, Nhat P, Law, Chris, Araujo, Flavia RB, Martin, Vincent JJ, Piekny, Alisa · 2024 · eLife