The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Directed Evolution of an RNA Enzyme
Abstract
An in vitro evolution procedure was used to obtain RNA enzymes with a particular catalytic function. A population of 10 13 variants of the Tetrahymena ribozyme, a group I ribozyme that catalyzes sequence-specific cleavage of RNA via a phosphoester transfer mechanism, was generated. This enzyme has a limited ability to cleave DNA under conditions of high temperature or high MgCl 2 concentration, or both. A selection constraint was imposed on the population of ribozyme variants such that only those individuals that carried out DNA cleavage under physiologic conditions were amplified to produce "progeny" ribozymes. Mutations were introduced during amplification to maintain heterogeneity in the population. This process was repeated for ten successive generations, resulting in enhanced (100 times) DNA cleavage activity.
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Citations
Cites
Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase
Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., & Erlich, H. A. (1988) · Science · doi:10.1126/science.2448875 · Cited by 17,719
Saiki, R. K., Scharf, S., Faloona, F., Mullis, K. B., Horn, G. T., Erlich, H. A., & Arnheim, N. (1985) · Science · doi:10.1126/science.2999980 · Cited by 8,956
An extracellular Darwinian experiment with a self-duplicating nucleic acid molecule.
Mills, D. R., Peterson, R. L., & Spiegelman, S. (1967) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.58.1.217 · Cited by 648
Cited by
Isolation of New Ribozymes from a Large Pool of Random Sequences
Bartel, D. P., & Szostak, J. W. (1993) · Science · doi:10.1126/science.7690155 · Cited by 900
Segré, D., Ben-Eli, D., Deamer, D. W., & Lancet, D. (2001) · Origins of life and evolution of the biosphere · doi:10.1023/a:1006746807104 · Cited by 620
Chemical etiology of nucleic acid structure
Eschenmoser, A. (1999) · Science · doi:10.1126/science.284.5423.2118 · Cited by 611
The RNA World: molecular cooperation at the origins of life
Higgs, P. G., & Lehman, N. (2015) · Nature Reviews Genetics · doi:10.1038/nrg3841 · Cited by 604
Deamer, D., Dworkin, J. P., Sandford, S. A., Bernstein, M. P., & Allamandola, L. J. (2002) · Astrobiology · doi:10.1089/153110702762470482 · Cited by 270
Metadata
- Authors
- Beaudry, A. A., & Joyce, G. F.
- Venue
- Science
- Published
- 1992-07-31
- Volume
- 257
- Issue
- 5070
- Pages
- 635-641
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1973352434
- Cited by
- 447
- References
- 3
- FWCI
- 13.87
- Type
- journal article
- Language
- en
- Open access
- closed
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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