The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Directed Evolution of an RNA Enzyme
Abstract
An in vitro evolution procedure was used to obtain RNA enzymes with a particular catalytic function. A population of 10 13 variants of the Tetrahymena ribozyme, a group I ribozyme that catalyzes sequence-specific cleavage of RNA via a phosphoester transfer mechanism, was generated. This enzyme has a limited ability to cleave DNA under conditions of high temperature or high MgCl 2 concentration, or both. A selection constraint was imposed on the population of ribozyme variants such that only those individuals that carried out DNA cleavage under physiologic conditions were amplified to produce "progeny" ribozymes. Mutations were introduced during amplification to maintain heterogeneity in the population. This process was repeated for ten successive generations, resulting in enhanced (100 times) DNA cleavage activity.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase
Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., & Erlich, H. A. (1988) · Science · doi:10.1126/science.2448875 · Cited by 17,719
Saiki, R. K., Scharf, S., Faloona, F., Mullis, K. B., Horn, G. T., Erlich, H. A., & Arnheim, N. (1985) · Science · doi:10.1126/science.2999980 · Cited by 8,956
An extracellular Darwinian experiment with a self-duplicating nucleic acid molecule.
Mills, D. R., Peterson, R. L., & Spiegelman, S. (1967) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.58.1.217 · Cited by 648
Cited by
Isolation of New Ribozymes from a Large Pool of Random Sequences
Bartel, D. P., & Szostak, J. W. (1993) · Science · doi:10.1126/science.7690155 · Cited by 900
Segré, D., Ben-Eli, D., Deamer, D. W., & Lancet, D. (2001) · Origins of life and evolution of the biosphere · doi:10.1023/a:1006746807104 · Cited by 620
Chemical etiology of nucleic acid structure
Eschenmoser, A. (1999) · Science · doi:10.1126/science.284.5423.2118 · Cited by 611
The RNA World: molecular cooperation at the origins of life
Higgs, P. G., & Lehman, N. (2015) · Nature Reviews Genetics · doi:10.1038/nrg3841 · Cited by 604
Deamer, D., Dworkin, J. P., Sandford, S. A., Bernstein, M. P., & Allamandola, L. J. (2002) · Astrobiology · doi:10.1089/153110702762470482 · Cited by 270
Wolf, Y. I., & Koonin, E. V. (2007) · Biology Direct · doi:10.1186/1745-6150-2-14 · Cited by 235
“It is noteworthy that the main approach to developing ribozymes with desired activities is in vitro selection that, at least conceptually, mimics the Darwinian evolution of ribozymes thought to occur in the primeval RNA world [49,50].”
BackgroundMirror symmetry breaking at the molecular level.
Avetisov, V, & Goldanskii, V (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.21.11435 · Cited by 206
Selection of RNA amide synthases
Wiegand, T. W., Janssen, R. C., & Eaton, B. E. (1997) · Chemistry & Biology · doi:10.1016/s1074-5521(97)90223-4 · Cited by 153
Lipid-like Self-Assembling Peptides
Zhang, S. (2012) · Accounts of Chemical Research · doi:10.1021/ar300034v · Cited by 132
Generalized RNA-directed recombination of RNA
Riley, C. A., & Lehman, N. (2003) · Chemistry & Biology · doi:10.1016/j.chembiol.2003.11.015 · Cited by 62
The search for missing links between self-replicating nucleic ACIDs and the RNA world
James, K. D., & Ellington, A. D. (1995) · Origins of life and evolution of the biosphere · doi:10.1007/bf01582021 · Cited by 39
Origins of life: a route to nanotechnology
Sowerby, S. J., Holm, N. G., & Petersen, G. B. (2001) · Biosystems · doi:10.1016/s0303-2647(01)00130-7 · Cited by 34
Joyce, G. F. (2009) · Cold Spring Harbor Symposia on Quantitative Biology · doi:10.1101/sqb.2009.74.004 · Cited by 31
Self-citationThe progene hypothesis: the nucleoprotein world and how life began
Altstein, A. D. (2015) · Biology Direct · doi:10.1186/s13062-015-0096-z · Cited by 20
(2008) · doi:10.1017/cbo9780511536557.022 · Cited by 4
Metadata
- Authors
- Beaudry, A. A., & Joyce, G. F.
- Venue
- Science
- Published
- 1992-07-31
- Volume
- 257
- Issue
- 5070
- Pages
- 635-641
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1973352434
- Cited by
- 447
- References
- 3
- FWCI
- 13.87
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
