Identification of a reservoir for HIV-1 in patients on highly active antiretroviral therapy
Abstract
The hypothesis that quiescent CD4+ T lymphocytes carrying proviral DNA provide a reservoir for human immunodeficiency virus-type 1 (HIV-1) in patients on highly active antiretroviral therapy (HAART) was examined. In a study of 22 patients successfully treated with HAART for up to 30 months, replication-competent virus was routinely recovered from resting CD4+ T lymphocytes. The frequency of resting CD4+ T cells harboring latent HIV-1 was low, 0.2 to 16.4 per 10(6) cells, and, in cross-sectional analysis, did not decrease with increasing time on therapy. The recovered viruses generally did not show mutations associated with resistance to the relevant antiretroviral drugs. This reservoir of nonevolving latent virus in resting CD4+ T cells should be considered in deciding whether to terminate treatment in patients who respond to HAART.
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After entering a controlled, chronic phase of HIV-1 infection, the main viral reservoir of intact proviral genomes is expected to reside in memory T cell subsets that persist for years.
“after entering a controlled, chronic phase of HIV-1 infection, the main viral reservoir of intact proviral genomes is expected to reside in memory T cell subsets that persist for years. 10-12”
From Revealing viral and cellular dynamics of HIV-1 at the single-cell level during early treatment periods (2023) · cites this paper as [10]
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- Authors
- Finzi, D., Hermankova, M., Pierson, T., Carruth, L. M., Buck, C., Chaisson, R. E., Quinn, T. C., Chadwick, K., Margolick, J., Brookmeyer, R., Gallant, J., Markowitz, M., Ho, D. D., Richman, D. D., & Siliciano, R. F.
- Affiliations
- Johns Hopkins University (US)
- D. Finzi, M. Hermankova, T. Pierson, L. M. Carruth, C. Buck, R. E. Chaisson, T. C. Quinn, J. Gallant, R. F. Siliciano, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
- Corresponding author
- Siliciano, Robert F.
- ORCID
- Venue
- Science
- Published
- 1997-11-14
- Volume
- 278
- Issue
- 5341
- Pages
- 1295-1300
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2139250164
- Cited by
- 3,098
- References
- 1
- Citations by year
- 2026: 81 · 2025: 102 · 2024: 103 · 2023: 115 · 2022: 118 · 2021: 124 · 2020: 160 · 2019: 125 · 2018: 140 · 2017: 117 · 2016: 108 · 2015: 115 · 2014: 110 · 2013: 99 · 2012: 85
- FWCI
- 45.97
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01ai023871-03)
- National Institute of Allergy and Infectious Diseases (AI-28108, AI23871, U01AI027670, AI2-7670)
- Topics
- Life Sciences › Immunology and Microbiology › Virology › HIV Research and Treatment
- Health Sciences › Medicine › Infectious Diseases › HIV/AIDS drug development and treatment
- Health Sciences › Medicine › Surgery › Infectious Diseases and Tuberculosis
- Keywords
- HIV-1 reservoir, latent HIV-1, proviral DNA, highly active antiretroviral therapy, replication-competent virus, antiretroviral resistance, treatment discontinuation, viral persistence
- MeSH terms
- Cell Separation, Cross-Sectional Studies, Drug Resistance, Microbial, Drug Therapy, Combination, Humans, Immunologic Memory, Lymphocyte Activation, Mutation, Proviruses, RNA, Viral, Time Factors, Viremia, Virus Replication, CD4-Positive T-Lymphocytes, HIV-1, HIV Infections, Virus Integration, Virus Latency, Anti-HIV Agents, Viral Load
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