Cited by 466Telomeres, Telomerase, and SenescenceAnimal Genetics and ReproductionPluripotent Stem Cells Research

Extension of Cell Life-Span and Telomere Length in Animals Cloned from Senescent Somatic Cells

Lanza, Robert P., Cibelli, Jose B., Blackwell, Catherine, Cristofalo, Vincent J., Francis, Mary Kay, Baerlocher, Gabriela M., Mak, Jennifer, Schertzer, Michael +4 more·2000·Science

Abstract

The potential of cloning depends in part on whether the procedure can reverse cellular aging and restore somatic cells to a phenotypically youthful state. Here, we report the birth of six healthy cloned calves derived from populations of senescent donor somatic cells. Nuclear transfer extended the replicative life-span of senescent cells (zero to four population doublings remaining) to greater than 90 population doublings. Early population doubling level complementary DNA-1 (EPC-1, an age-dependent gene) expression in cells from the cloned animals was 3.5- to 5-fold higher than that in cells from age-matched (5 to 10 months old) controls. Southern blot and flow cytometric analyses indicated that the telomeres were also extended beyond those of newborn (<2 weeks old) and age-matched control animals. The ability to regenerate animals and cells may have important implications for medicine and the study of mammalian aging.

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Crossref · 10.1126/science.288.5466.665

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  • Cloning from senescent fibroblasts of old cows produced calves whose fibroblasts had regained population doubling and better telomere maintenance than age-matched controls.

    “Somatic cell nuclear transfer of senescent fibroblasts from aged bovine donors results in cloned calves with fibroblasts that have restored population doubling and increased telomere maintenance compared to age-matched control animals180.”

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Authors
Lanza, R. P., Cibelli, J. B., Blackwell, C., Cristofalo, V. J., Francis, M. K., Baerlocher, G. M., Mak, J., Schertzer, M., Chavez, E. A., Sawyer, N., Lansdorp, P. M., & West, M. D.
Affiliations
  • Advanced Cell Technology (United States) (US)
  • Thomas Jefferson University (US)
  • Terry Fox Research Institute (CA)
  • University of British Columbia (CA)
Corresponding author
Lanza, Robert P.
Venue
Science
Published
2000-04-28
Volume
288
Issue
5466
Pages
665-669
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
OpenAlex
W2141926462
Cited by
466
References
44
Citations by year
2025: 4 · 2024: 2 · 2023: 10 · 2022: 8 · 2021: 10 · 2020: 4 · 2019: 9 · 2018: 3 · 2017: 7 · 2016: 7 · 2015: 7 · 2014: 13 · 2013: 16 · 2012: 16
FWCI
34.86
Type
journal article
Language
en
Open access
closed
Locations
  • Science (publishedVersion)
  • PubMed (publishedVersion)
  • ScholarWorks@UMassAmherst (University of Massachusetts Amherst) (submittedVersion)
Funders
  • National Institutes of Health (1r01gm056162-01)
  • National Institute on Aging (AG00378)
  • National Institute of General Medical Sciences (GM56162)
  • National Institute of Allergy and Infectious Diseases (AI 29524)
Topics
  • Health Sciences › Medicine › Physiology › Telomeres, Telomerase, and Senescence
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Animal Genetics and Reproduction
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
Keywords
somatic cell nuclear transfer, cloning, cellular aging, replicative lifespan, telomere length, telomere elongation, cloned calves, age-related gene expression, regenerative medicine, mammalian aging
MeSH terms
Pigment Epithelium-Derived Factor, Animals, Cattle, Cell Division, Cells, Cultured, Clone Cells, Embryo Transfer, Eye Proteins, Female, Fibroblasts, Flow Cytometry, Longevity, Nerve Growth Factors, Proteins, RNA, Messenger, Blotting, Southern, Serpins, Matched-Pair Analysis, Telomere, Cellular Senescence, In Situ Hybridization, Fluorescence, DNA, Complementary, Cloning, Organism, Nuclear Transfer Techniques

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