Orchestrated Transcription of Key Pathways in Arabidopsis by the Circadian Clock
Abstract
Like most organisms, plants have endogenous biological clocks that coordinate internal events with the external environment. We used high-density oligonucleotide microarrays to examine gene expression in Arabidopsis and found that 6% of the more than 8000 genes on the array exhibited circadian changes in steady-state messenger RNA levels. Clusters of circadian-regulated genes were found in pathways involved in plant responses to light and other key metabolic pathways. Computational analysis of cycling genes allowed the identification of a highly conserved promoter motif that we found to be required for circadian control of gene expression. Our study presents a comprehensive view of the temporal compartmentalization of physiological pathways by the circadian clock in a eukaryote.
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SsAQP2 is the first plasma membrane aquaporin shown to be regulated both diurnally and by the circadian clock.
“SsAQP2 is the first plasma membrane aquaporin shown to be regulated both diurnally and by the circadian clock.”
From Plasma Membrane Aquaporins in the Motor Cells of <i>Samanea saman</i>: Diurnal and Circadian Regulation (2002) · cites this paper as Harmer et al., 2000
δ-TIP was the first aquaporin gene in any organism shown to be regulated in a circadian manner.
“becoming the first aquaporin gene in any organism shown to be regulated in this manner.”
From Plasma Membrane Aquaporins in the Motor Cells of <i>Samanea saman</i>: Diurnal and Circadian Regulation (2002) · cites this paper as Harmer et al., 2000
The mRNA level of an Arabidopsis tonoplast aquaporin, δ-TIP, cycles in a circadian rhythm.
“the mRNA level of an Arabidopsis tonoplast aquaporin, δ-TIP, was shown to cycle in a circadian rhythm (Harmer et al., 2000)”
From Plasma Membrane Aquaporins in the Motor Cells of <i>Samanea saman</i>: Diurnal and Circadian Regulation (2002) · cites this paper as Harmer et al., 2000
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- Authors
- Harmer, S. L., Hogenesch, J. B., Straume, M., Chang, H.-S., Han, B., Zhu, T., Wang, X., Kreps, J. A., & Kay, S. A.
- Affiliations
- Scripps Research Institute (US)
- Genomics Institute of the Novartis Research Foundation (US)
- University of Virginia (US)
- Novartis Agricultural Discovery Institute, 3115 Merryfield Row, San Diego, CA 92121, USA.
- Corresponding author
- Kay, Steve A.
- Venue
- Science
- Published
- 2000-12-15
- Volume
- 290
- Issue
- 5499
- Pages
- 2110-2113
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1995815904
- Cited by
- 1,640
- References
- 0
- Citations by year
- 2026: 26 · 2025: 53 · 2024: 33 · 2023: 25 · 2022: 57 · 2021: 68 · 2020: 41 · 2019: 49 · 2018: 50 · 2017: 69 · 2016: 46 · 2015: 58 · 2014: 65 · 2013: 68 · 2012: 71
- FWCI
- 131.88
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01dk051562-23)
- National Institute of General Medical Sciences (5F32GM20118-02, F32GM020118)
- National Institute of Diabetes and Digestive and Kidney Diseases (R01DK 51562)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Circadian rhythm and melatonin
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Light effects on plants
- Keywords
- circadian clock, circadian gene expression, Arabidopsis thaliana, mRNA levels, light response, metabolic pathways, promoter elements, temporal gene regulation, transcriptional regulation
- MeSH terms
- Biological Clocks, Circadian Rhythm, Light, Nitrogen, Photosynthesis, Promoter Regions, Genetic, RNA, Messenger, Sulfur, Transcription, Genetic, Genes, Plant, Arabidopsis, Gene Expression Regulation, Plant, RNA, Plant, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling, Plants, Genetically Modified, Photosynthetic Reaction Center Complex Proteins, Carbohydrate Metabolism
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