Cited by 175Cholesterol and Lipid MetabolismLipoproteins and Cardiovascular HealthLipid metabolism and disorders

Diet-induced hypercholesterolemia in mice: prevention by overexpression of LDL receptors

Yokode, Masayuki, Hammer, Robert E., Ishibashi, Shun, Brown, Michael S., Goldstein, Joseph L.·1990·Science

Abstract

The current studies were designed to determine whether chronic overexpression of low density lipoprotein (LDL) receptors in the liver would protect mice from the increase in plasma LDL-cholesterol that is induced by high-fat diets. A line of transgenic mice was studied that express the human LDL receptor gene in the liver under control of the transferrin promoter. When fed a diet containing cholesterol, saturated fat, and bile acids for 3 weeks, the transgenic mice, in contrast to normal mice, did not develop a detectable increase in plasma LDL. The current data indicate that unregulated overexpression of LDL receptors can protect against diet-induced hypercholesterolemia in mice.

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Crossref · 10.1126/science.2244210

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Authors
Yokode, M., Hammer, R. E., Ishibashi, S., Brown, M. S., & Goldstein, J. L.
Affiliations
  • The University of Texas Southwestern Medical Center (US)
  • Howard Hughes Medical Institute (US)
Venue
Science
Published
1990-11-30
Volume
250
Issue
4985
Pages
1273-1275
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
OpenAlex
W2059352674
Cited by
175
References
28
Citations by year
2024: 1 · 2021: 3 · 2020: 1 · 2019: 4 · 2017: 1 · 2016: 2 · 2015: 2 · 2014: 2 · 2012: 3
FWCI
18.32
Type
journal article
Language
en
Open access
closed
Locations
  • Science (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Heart, Lung, and Blood Institute (HL-20948, P01HL020948)
Topics
  • Health Sciences › Medicine › Surgery › Cholesterol and Lipid Metabolism
  • Health Sciences › Medicine › Surgery › Lipoproteins and Cardiovascular Health
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Lipid metabolism and disorders
Keywords
LDL receptor, hypercholesterolemia, high-fat diet, transgenic mice, liver, LDL cholesterol, bile acids, cholesterol, saturated fat, gene overexpression
MeSH terms
Animals, Cholesterol, Dietary, Dietary Fats, Exons, Humans, Hypercholesterolemia, Introns, Lipoproteins, Lipoproteins, HDL, Cholesterol, LDL, Lipoproteins, VLDL, Liver, Mice, Inbred C57BL, Mice, Transgenic, Nucleic Acid Hybridization, Promoter Regions, Genetic, Receptors, LDL, RNA, Messenger, Transferrin, Gene Expression, Mice, Lipoproteins, IDL

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