The DOI matches its Crossref record; 37 of the 37 references it cites were found in a registry or an index.
Sequence and Expression of Human Estrogen Receptor Complementary DNA
Abstract
The mechanism by which the estrogen receptor and other steroid hormone receptors regulate gene expression in eukaryotic cells is not well understood. In this study, a complementary DNA clone containing the entire translated portion of the messenger RNA for the estrogen receptor from MCF-7 human breast cancer cells was sequenced and then expressed in Chinese hamster ovary (CHO-K1) cells to give a functional protein. An open reading frame of 1785 nucleotides in the complementary DNA corresponded to a polypeptide of 595 amino acids and a molecular weight of 66,200, which is in good agreement with published molecular weight values of 65,000 to 70,000 for the estrogen receptor. Homogenates of transformed Chinese hamster ovary cells contained a protein that bound [ 3 H]estradiol and sedimented as a 4S complex in salt-containing sucrose gradients and as an 8 to 9S complex in the absence of salt. Interaction of this receptor-[ 3 H]estradiol complex with a monoclonal antibody that is specific for primate ER confirms the identity of the expressed complementary DNA as human estrogen receptor. Amino acid sequence comparisons revealed significant regional homology among the human estrogen receptor, the human glucocorticoid receptor, and the putative v- erb A oncogene product. This suggests that steroid receptor genes and the avian erythroblastosis viral oncogene are derived from a common primordial gene. The homologous region, which is rich in cysteine, lysine, and arginine, may represent the DNA-binding domain of these proteins.
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Cites
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Primary structure and expression of a functional human glucocorticoid receptor cDNA
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Monoclonal antibodies localize oestrogen receptor in the nuclei of target cells
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Self-citationA two-step mechanism for the interaction of estradiol with rat uterus.
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GENETICS OF SOMATIC MAMMALIAN CELLS
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Cited by
ERβ: Identification and characterization of a novel human estrogen receptor
Mosselman, S., Polman, J., & Dijkema, R. (1996) · FEBS Letters · doi:10.1016/0014-5793(96)00782-x · Cited by 2,269
Melanin pigmentation in mammalian skin and its hormonal regulation
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Methylation of the oestrogen receptor CpG island links ageing and neoplasia in human colon
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Multiple Actions of Steroid Hormones—A Focus on Rapid, Nongenomic Effects
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Metadata
- Authors
- Greene, G. L., Gilna, P., Waterfield, M., Baker, A., Hort, Y., & Shine, J.
- Venue
- Science
- Published
- 1986-03-07
- Volume
- 231
- Issue
- 4742
- Pages
- 1150-1154
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2065785294
- Cited by
- 1,311
- References
- 37
- FWCI
- 52.21
- Type
- journal article
- Language
- en
- Open access
- closed
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- Greene, G. L., Gilna, P., Waterfield, M., Baker, A., Hort, Y., & Shine, J. (1986). Sequence and Expression of Human Estrogen Receptor Complementary DNA. Science. https://doi.org/10.1126/science.3753802
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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