The DOI matches its Crossref record; 20 of the 20 references it cites were found in a registry or an index.
Expression in Escherichia coli of a Chemically Synthesized Gene for the Hormone Somatostatin
Abstract
A gene for somatostatin, a mammalian peptide (14 amino acid residues) hormone, was synthesized by chemical methods. This gene was fused to the Escherichia coli β-galactosidase gene on the plasmid pBR322. Transformation of E. coli with the chimeric plasmid DNA led to the synthesis of a polypeptide including the sequence of amino acids corresponding to somatostatin. In vitro, active somatostatin was specifically cleaved from the large chimeric protein by treatment with cyanogen bromide. This represents the first synthesis of a functional polypeptide product from a gene of chemically synthesized origin.
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Cites
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Self-citationConstruction and characterization of new cloning vehicle. II. A multipurpose cloning system
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Self-citationHypothalamic Polypeptide That Inhibits the Secretion of Immunoreactive Pituitary Growth Hormone
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Rat Insulin Genes: Construction of Plasmids Containing the Coding Sequences
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[27] The cyanogen bromide reaction
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Cited by
Recombinant protein expression in Escherichia coli: advances and challenges
Rosano, G. L., & Ceccarelli, E. A. (2014) · Frontiers in Microbiology · doi:10.3389/fmicb.2014.00172 · Cited by 2,556
Codon bias and heterologous protein expression
Gustafsson, C., Govindarajan, S., & Minshull, J. (2004) · Trends in Biotechnology · doi:10.1016/j.tibtech.2004.04.006 · Cited by 1,330
The discovery of receptor tyrosine kinases: targets for cancer therapy
Gschwind, A., Fischer, O. M., & Ullrich, A. (2004) · Nature Reviews Cancer · doi:10.1038/nrc1360 · Cited by 1,227
Expression in Escherichia coli of chemically synthesized genes for human insulin.
Goeddel, D. V., Kleid, D. G., Bolivar, F., Heyneker, H. L., Yansura, D. G., Crea, R., Hirose, T., Kraszewski, A., Itakura, K., & Riggs, A. D. (1979) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.76.1.106 · Cited by 869
Self-citationThe purification of eukaryotic polypeptides synthesized in Escherichia coli
Marston, F. A. (1986) · Biochemical Journal · doi:10.1042/bj2400001 · Cited by 836
Metadata
- Authors
- Itakura, K., Hirose, T., Crea, R., Riggs, A. D., Heyneker, H. L., Bolivar, F., & Boyer, H. W.
- Venue
- Science
- Published
- 1977-12-09
- Volume
- 198
- Issue
- 4321
- Pages
- 1056-1063
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1995013658
- Cited by
- 853
- References
- 20
- FWCI
- 28.58
- Type
- journal article
- Language
- en
- Open access
- closed
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- Itakura, K., Hirose, T., Crea, R., Riggs, A. D., Heyneker, H. L., Bolivar, F., & Boyer, H. W. (1977). Expression in Escherichia coli of a Chemically Synthesized Gene for the Hormone Somatostatin. Science. https://doi.org/10.1126/science.412251
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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