Determinants of attenuation and temperature sensitivity in the type 1 poliovirus Sabin vaccine
Open accessAbstract
To identify determinants of attenuation in the poliovirus type 1 Sabin vaccine strain, a series of recombinant viruses were constructed by using infectious cDNA clones of the virulent type 1 poliovirus P1/Mahoney and the attenuated type 1 vaccine strain P1/Sabin. Intracerebral inoculation of these viruses into transgenic mice which express the human receptor for poliovirus identified regions of the genome that conferred reduced neurovirulence. Exchange of smaller restriction fragments and site-directed mutagenesis were used to identify the nucleotide changes responsible for attenuation. P1/Sabin mutations at nucleotides 935 of VP4, 2438 of VP3, and 2795 and 2879 of VP1 were all shown to be determinants of attenuation. The recombinant viruses and site-directed mutants were also used to identify the nucleotide changes which are involved in the temperature sensitivity of P1/Sabin. Determinants of this phenotype in HeLa cells were mapped to changes at nucleotides 935 of VP4, 2438 of VP3, and 2741 of VP1. The 3Dpol gene of P1/Sabin, which contains three amino acid differences from its parent P1/Mahoney, also contributes to the temperature sensitivity of P1/Sabin; however, mutants containing individual amino acid changes grew as well as P1/Mahoney at elevated temperatures, suggesting that either some combination or all three changes are required for temperature sensitivity. In addition, the 3'-noncoding region of P1/Sabin augments the temperature-sensitive phenotype conferred by 3Dpol. Although nucleotide 2741, 3Dpol, and the 3'-noncoding region of P1/Sabin contribute to the temperature sensitivity of P1/Sabin, they do not contribute to attenuation in transgenic mice expressing the poliovirus receptor, demonstrating that determinants of attenuation and temperature sensitivity can be genetically separated.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The text we found for this paper looks like another document, so the self-check wasn’t run.
Cites
Molecular Cloning: A Laboratory Manual
Sambrook, J., Fritsch, E., & Maniatis, T. (1989) · UCLA - Biblioteca de Ciencias de la Salud · Cited by 133,589 · see citing works
A SIMPLE METHOD OF ESTIMATING FIFTY PER CENT ENDPOINTS12
REED, L. J., & Muench, H. (1938) · American Journal of Epidemiology · doi:10.1093/oxfordjournals.aje.a118408 · Cited by 20,341 · see citing works
A simple method of estimating 50 per cent end points
Muench, H. (1938) · Medical Entomology and Zoology · Cited by 8,377 · see citing works
Three-Dimensional Structure of Poliovirus at 2.9 Å Resolution
Hogle, J. M., Chow, M., & Filman, D. J. (1985) · Science · doi:10.1126/science.2994218 · Cited by 1,262 · see citing works
Mendelsohn, C. L., Wimmer, E., & Racaniello, V. R. (1989) · Cell · doi:10.1016/0092-8674(89)90690-9 · Cited by 1,116 · see citing works
Self-citationA simple method of estimating fifty per cent end-points.
Reed, L. (1938) · Medical Entomology and Zoology · Cited by 925 · see citing works
Cloned Poliovirus Complementary DNA Is Infectious in Mammalian Cells
Racaniello, V. R., & Baltimore, D. (1981) · Science · doi:10.1126/science.6272391 · Cited by 696 · see citing works
Self-citationRacaniello, V. R., & Baltimore, D. (1981) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.78.8.4887 · Cited by 555 · see citing works
Self-citationWimmer, E., Hellen, C. U. T., & Cao, X. (1993) · Annual Review of Genetics · doi:10.1146/annurev.ge.27.120193.002033 · Cited by 554 · see citing works
Transgenic mice expressing a human poliovirus receptor: A new model for poliomyelitis
Ren, R., Costantini, F. D., Gorgacz, E. J., Lee, J. J., & Racaniello, V. R. (1990) · Cell · doi:10.1016/0092-8674(90)90168-e · Cited by 379 · see citing works
Self-citationFilman, D. J., Syed, R., Chow, M., Macadam, A. J., Minor, P. D., & Hogle, J. M. (1989) · The EMBO Journal · doi:10.1002/j.1460-2075.1989.tb03541.x · Cited by 364 · see citing works
History of Sabin attenuated poliovirus oral live vaccine strains
Sabin, A. B., & Boulger, L. R. (1973) · Journal of Biological Standardization · doi:10.1016/0092-1157(73)90048-6 · Cited by 318 · see citing works
Complete nucleotide sequence of the attenuated poliovirus Sabin 1 strain genome.
Nomoto, A., Omata, T., Toyoda, H., Kuge, S., Horie, H., Kataoka, Y., Genba, Y., Nakano, Y., & Imura, N. (1982) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.79.19.5793 · Cited by 308 · see citing works
Transgenic mice susceptible to poliovirus.
Koike, S., Taya, C., Kurata, T., Abe, S., Ise, I., Yonekawa, H., & Nomoto, A. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.3.951 · Cited by 294 · see citing works
Vaccine-associated paralytic poliomyelitis. United States: 1973 through 1984
Nkowane, B. M. (1987) · JAMA · doi:10.1001/jama.257.10.1335 · Cited by 240 · see citing works
Kawamura, N., Kohara, M., Abe, S., Komatsu, T., Tago, K., Arita, M., & Nomoto, A. (1989) · Journal of Virology · doi:10.1128/jvi.63.3.1302-1309.1989 · Cited by 232 · see citing works
Genetic basis of attenuation of the Sabin type 3 oral poliovirus vaccine
Westrop, G. D., Wareham, K. A., Evans, D. M. A., Dunn, G., Minor, P. D., Magrath, D. I., Taffs, F., Marsden, S. A., Skinner, M. A., & Schild, G. C. (1989) · Journal of Virology · doi:10.1128/jvi.63.3.1338-1344.1989 · Cited by 232 · see citing works
Mapping of sequences required for mouse neurovirulence of poliovirus type 2 Lansing
Monica, N. L., Meriam, C., & Racaniello, V. R. (1986) · Journal of Virology · doi:10.1128/jvi.57.2.515-525.1986 · Cited by 198 · see citing works
Self-citationGenetic analysis of the attenuation phenotype of poliovirus type 1
Omata, T., Kohara, M., Kuge, S., Komatsu, T., Abe, S., Semler, B. L., Kameda, A., Itoh, H., Arita, M., & Wimmer, E. (1986) · Journal of Virology · doi:10.1128/jvi.58.2.348-358.1986 · Cited by 190 · see citing works
Identification of two determinants that attenuate vaccine-related type 2 poliovirus
Ren, R., Moss, E. G., & Racaniello, V. R. (1991) · Journal of Virology · doi:10.1128/jvi.65.3.1377-1382.1991 · Cited by 164 · see citing works
Self-citationMurray, M. G., Bradley, J., Yang, X.-F., Wimmer, E., Moss, E. G., & Racaniello, V. R. (1988) · Science · doi:10.1126/science.2838906 · Cited by 159 · see citing works
Self-citationSTUDIES ON VARIANTS OF POLIOMYELITIS VIRUS
Sabin, A. B., Hennessen, W., & Winsser, J. (1954) · The Journal of Experimental Medicine · doi:10.1084/jem.99.6.551 · Cited by 155 · see citing works
Reversion to neurovirulence of the live-attenuated Sabin type 3 oral pollovirus vaccine
Cann, A. J., Stanway, G., Hughes, P. J., Minor, P. D., Evans, D. M. A., Schild, G. C., & Almond, J. W. (1984) · Nucleic Acids Research · doi:10.1093/nar/12.20.7787 · Cited by 146 · see citing works
Human poliovirus receptor gene expression and poliovirus tissue tropism in transgenic mice
Ren, R., & Racaniello, V. R. (1992) · Journal of Virology · doi:10.1128/jvi.66.1.296-304.1992 · Cited by 142 · see citing works
Self-citationGenetic basis of attenuation of the Sabin type-2 vaccine strain of poliovirus in primates.
Macadam, A. J., Pollard, S. R., Ferguson, G., Skuce, R., Wood, D., Almond, J. W., & Minor, P. D. (1993) · Virology · doi:10.1006/viro.1993.1003 · Cited by 139 · see citing works
Martin, A., Wychowski, C., Couderc, T., Crainic, R., Hogle, J. M., & Girard, M. P. (1988) · The EMBO Journal · doi:10.1002/j.1460-2075.1988.tb03140.x · Cited by 132 · see citing works
Macadam, A. J., Arnold, C., Howlett, J., John, A., Marsden, S., Taffs, F., Reeve, P., Hamada, N., Wareham, K., Almond, J., et al. (1989) · Virology · doi:10.1016/0042-6822(89)90183-9 · Cited by 125 · see citing works
Vaccine-Associated Paralytic Poliomyelitis
Beausoleil, J. L., Nordgren, R. E., & Modlin, J. F. (1994) · Journal of Child Neurology · doi:10.1177/088307389400900324 · Cited by 107 · see citing works
Christodoulou, C., Colbère-Garapin, F., Macadam, A. J., Taffs, L. F., Marsden, S. A., Minor, P. D., & Horaud, F. (1990) · Journal of Virology · doi:10.1128/jvi.64.10.4922-4929.1990 · Cited by 106 · see citing works
Colston, E., & Racaniello, V. R. (1994) · The EMBO Journal · doi:10.1002/j.1460-2075.1994.tb06930.x · Cited by 104 · see citing works
Self-citationHorie, H., Koike, S., Kurata, T., Sato-Yoshida, Y., Ise, I., Ota, Y., Abe, S., Hioki, K., Kato, H., & Taya, C. (1994) · Journal of Virology · doi:10.1128/jvi.68.2.681-688.1994 · Cited by 96 · see citing works
Persistent poliovirus infection of human neuroblastoma cells.
Colbère-Garapin, F., Christodoulou, C., Crainic, R., & Pelletier, I. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.19.7590 · Cited by 95 · see citing works
Analysis of RNA synthesis of type 1 poliovirus by using an in vitro molecular genetic approach
Toyoda, H., Yang, C. F., Takeda, N., Nomoto, A., & Wimmer, E. (1987) · Journal of Virology · doi:10.1128/jvi.61.9.2816-2822.1987 · Cited by 81 · see citing works
Restricted growth of attenuated poliovirus strains in cultured cells of a human neuroblastoma
Agol, V. I., Drozdov, S. G., Ivannikova, T. A., Kolesnikova, M. S., Korolev, M. B., & Tolskaya, E. A. (1989) · Journal of Virology · doi:10.1128/jvi.63.9.4034-4038.1989 · Cited by 81 · see citing works
Nucleotide sequence of a neurovirulent variant of the type 2 oral poliovirus vaccine
Pollard, S., Dunn, G., Cammack, N. C., Minor, P. D., & Almond, J. W. (1989) · Journal of Virology · doi:10.1128/jvi.63.11.4949-4951.1989 · Cited by 75 · see citing works
Colston, E. M., & Racaniello, V. R. (1995) · Journal of Virology · doi:10.1128/jvi.69.8.4823-4829.1995 · Cited by 75 · see citing works
Self-citationMorrison, M. E., He, Y. J., Wien, M. W., et al. (1994) · Journal of Virology · doi:10.1128/jvi.68.4.2578-2588.1994 · Cited by 74 · see citing works
Mapping of attenuating sequences of an avirulent poliovirus type 2 strain
Moss, E. G., O'Neill, R. E., & Racaniello, V. R. (1989) · Journal of Virology · doi:10.1128/jvi.63.5.1884-1890.1989 · Cited by 68 · see citing works
Self-citationA mutation in the RNA polymerase of poliovirus type 1 contributes to attenuation in mice
Tardy-Panit, M., Blondel, B., Martin, A., Tekaia, F., Horaud, F., & Delpeyroux, F. (1993) · Journal of Virology · doi:10.1128/jvi.67.8.4630-4638.1993 · Cited by 64 · see citing works
A mutation present in the amino terminus of Sabin 3 poliovirus VP1 protein is attenuating
Tatem, J. M., Weeks-Levy, C., Georgiu, A. F., Dimichele, S. J., Gorgacz, E. J., Racaniello, V. R., Cano, F. R., & Mento, S. J. (1992) · Journal of Virology · doi:10.1128/jvi.66.5.3194-3197.1992 · Cited by 62 · see citing works
Self-citationHost range determinants located on the interior of the poliovirus capsid.
Moss, E. G., & Racaniello, V. R. (1991) · The EMBO Journal · doi:10.1002/j.1460-2075.1991.tb08046.x · Cited by 57 · see citing works
Self-citationPersistent Infection of Human Erythroblastoid Cells by Poliovirus
Lloyd, R. E., & Bovée, M. (1993) · Virology · doi:10.1006/viro.1993.1250 · Cited by 50 · see citing works
Macadam, A. J., Ferguson, G. L., Arnold, C., & Minor, P. D. (1991) · Journal of Virology · doi:10.1128/jvi.65.10.5225-5231.1991 · Cited by 48 · see citing works
Couderc, T., GUEDO, N., Cálvez, V., et al. (1994) · Journal of Virology · doi:10.1128/jvi.68.12.8386-8391.1994 · Cited by 45 · see citing works
Monica, N. L., Klipsky, W. J., & Racaniello, V. R. (1987) · Virology · doi:10.1016/0042-6822(87)90136-x · Cited by 44 · see citing works
Self-citationMartin, A., Bénichou, D., Couderc, T., Hogle, J. M., Wychowski, C., van der Werf, S., & Girard, M. P. (1991) · Virology · doi:10.1016/0042-6822(91)90078-p · Cited by 35 · see citing works
Thermosensitive Block of the Sabin Strain of Poliovirus Type I
Fiszman, M. Y., Reynier, M., Bucchini, D., et al. (1972) · Journal of Virology · doi:10.1128/jvi.10.6.1143-1151.1972 · Cited by 26 · see citing works
(1982) · Europe PMC (PubMed Central) · Cited by 20 · see citing works
Couderc, T., Martin, A., Wychowski, C., et al. (1991) · Journal of General Virology · doi:10.1099/0022-1317-72-4-973 · Cited by 13 · see citing works
Susceptibility and resistance to poliovirus-induced paralysis of inbred mouse strains
Jubelt, B., Ropka, S. L., Goldfarb, S., et al. (1991) · Journal of Virology · doi:10.1128/jvi.65.2.1035-1040.1991 · Cited by 11 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
VACCINE-DERIVED POLIOVIRUSES AND THE ENDGAME STRATEGY FOR GLOBAL POLIO ERADICATION
Kew, O. M., Sutter, R. W., de Gourville, E. M., Dowdle, W. R., & Pallansch, M. A. (2005) · Annual Review of Microbiology · doi:10.1146/annurev.micro.58.030603.123625 · Cited by 703 · see citing works
Kew, O., Morris-Glasgow, V., Landaverde, M., Burns, C., Shaw, J., Garib, Z., André, J., Blackman, E., Freeman, C. J., Jorba, J., et al. (2002) · Science · doi:10.1126/science.1068284 · Cited by 600 · see citing works
Dual Stem Loops within the Poliovirus Internal Ribosomal Entry Site Control Neurovirulence
Gromeier, M., Bossert, B., Arita, M., Nomoto, A., & Wimmer, E. (1999) · Journal of Virology · doi:10.1128/jvi.73.2.958-964.1999 · Cited by 160 · see citing works
Circulation of type 1 vaccine-derived poliovirus in the philippines in 2001
Shimizu, H., Thorley, B., Paladin, F. J., Brussen, K. A., Stambos, V., Yuen, L., Utama, A., Tano, Y., Arita, M., Yoshida, H., et al. (2004) · Journal of Virology · doi:10.1128/jvi.78.24.13512-13521.2004 · Cited by 146 · see citing works
Polio eradication: the OPV paradox
Dowdle, W. R., De Gourville, E., Kew, O. M., Pallansch, M. A., & Wood, D. J. (2003) · Reviews in Medical Virology · doi:10.1002/rmv.401 · Cited by 144 · see citing works
Combelas, N., Holmblat, B., Joffret, M.-L., Colbère-Garapin, F., & Delpeyroux, F. (2011) · Viruses · doi:10.3390/v3081460 · Cited by 135 · see citing works
Bellmunt, A., May, G., Zell, R., Pring-Åkerblom, P., Verhagen, W., & Heim, A. (1999) · Virology · doi:10.1006/viro.1999.0003 · Cited by 128 · see citing works
Long-term circulation of vaccine-derived poliovirus that causes paralytic disease
Cherkasova, E. A., Korotkova, E. A., Yakovenko, M. L., Ivanova, O. E., Eremeeva, T. P., Chumakov, K. M., & Agol, V. I. (2002) · Journal of Virology · doi:10.1128/jvi.76.13.6791-6799.2002 · Cited by 103 · see citing works
Chapter 1 The Pathogenesis of Poliomyelitis: What We Don't Know
Nathanson, N. (2008) · Advances in Virus Research · doi:10.1016/s0065-3527(08)00001-8 · Cited by 95 · see citing works
Macadam, A. J., Ferguson, G., Stone, D. M., Meredith, J., Knowlson, S., Auda, G., Almond, J. W., & Minor, P. D. (2006) · Journal of Virology · doi:10.1128/jvi.00370-06 · Cited by 89 · see citing works
Georgescu, M. M., Balanant, J., Macadam, A., Otelea, D., Combiescu, M., Combiescu, A. A., Crainic, R., & Delpeyroux, F. (1997) · Journal of Virology · doi:10.1128/jvi.71.10.7758-7768.1997 · Cited by 83 · see citing works
Nucleotide variation in Sabin type 2 poliovirus from an immunodeficient patient with poliomyelitis
Buttinelli, G., Donati, V., Fiore, S., Marturano, J., Plebani, A., Balestri, P., Soresina, A. R., Vivarelli, R., Delpeyroux, F., Martin, J., et al. (2003) · Journal of General Virology · doi:10.1099/vir.0.18974-0 · Cited by 73 · see citing works
Mechanisms of genetic variation in polioviruses
Savolainen‐Kopra, C., & Blomqvist, S. (2010) · Reviews in Medical Virology · doi:10.1002/rmv.663 · Cited by 67 · see citing works
Paul, A. V., Mugavero, J., Yin, J., Hobson, S., Schultz, S., van Boom, J. H., & Wimmer, E. (2000) · Virology · doi:10.1006/viro.2000.0354 · Cited by 48 · see citing works
Poliovirus Vaccines: Molecular Biology and Immune Response
Minor, P. D., & Almond, J. (2014) · Molecular Biology of Picornavirus · doi:10.1128/9781555817916.ch30 · Cited by 33 · see citing works
Determinants of Poliovirus Pathogenesis
Gromeier, M., & Nomoto, A. (2014) · Molecular Biology of Picornavirus · doi:10.1128/9781555817916.ch29 · Cited by 20 · see citing works
- Authors
- Bouchard, M. J., Lam, D. H., & Racaniello, V. R.
- Affiliations
- Columbia University Irving Medical Center (US)
- ORCID
- Racaniello, V R
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 1995-08
- Volume
- 69
- Issue
- 8
- Pages
- 4972-4978
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- PMC
- PMC189313
- OpenAlex
- W2104799220
- Cited by
- 140
- References
- 50
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 4 · 2023: 3 · 2021: 1 · 2020: 5 · 2018: 6 · 2017: 1 · 2016: 3 · 2015: 8 · 2014: 5 · 2013: 2 · 2012: 5
- FWCI
- 6.43
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of Virology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Allergy and Infectious Diseases (AI20017)
- Topics
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Viral Infections and Immunology Research
- Health Sciences › Medicine › Epidemiology › Virology and Viral Diseases
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Virus Infections Studies
- Keywords
- poliovirus type 1, Sabin vaccine strains, temperature sensitivity, neurovirulence, VP4, VP3, VP1, 3' non-coding region, poliovirus receptor, transgenic mice, site-directed mutagenesis, recombinant virus
- MeSH terms
- Animals, Capsid, Female, Hot Temperature, HeLa Cells, Humans, Male, Mice, Transgenic, Nervous System Diseases, Poliovirus Vaccine, Oral, Recombination, Genetic, Vaccines, Attenuated, Virulence, Mutagenesis, Site-Directed, Poliovirus, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
