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Nanometer resolution imaging and tracking of fluorescent molecules with minimal photon fluxes
Open accessAbstract
Superresolution imaging in sharper focus: An optical microscope cannot distinguish objects separated by less than half the wavelength of light. Superresolution techniques have broken this “diffraction limit” and provided exciting new insights into cell biology. Still, such techniques hit a limit at a resolution of about 10 nm. Balzarotti et al. describe another way of localizing single molecules called MINFLUX (see the Perspective by Xiao and Ha). As in photoactivated localization microscopy and stochastic optical reconstruction microscopy, fluorophores are stochastically switched on and off, but the emitter is located using an excitation beam that is doughnut-shaped, as in stimulated emission depletion. Finding the point where emission is minimal reduces the number of photons needed to localize an emitter. MINFLUX attained ∼1-nanometer precision, and, in single-particle tracking, achieved a 100-fold enhancement in temporal resolution. Science , this issue p. 606 ; see also p. 582
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Citations
Cited by
Schmidt, R., Weihs, T., Wurm, C. A., Jansen, I., Rehman, J., Sahl, S. J., & Hell, S. W. (2021) · Nature Communications · doi:10.1038/s41467-021-21652-z · Cited by 306
Self-citation‘MINFLUX monitors rapid molecular jumps with superior spatiotemporal resolution’
Eilers, Y., Ta, H., Gwosch, K. C., Balzarotti, F., & Hell, S. W. (2018) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1801672115 · Cited by 188
Self-citationDirect observation of motor protein stepping in living cells using MINFLUX
Deguchi, T., Iwanski, M. K., Schentarra, E.-M., Heidebrecht, C., Schmidt, L., Heck, J., Weihs, T., Schnorrenberg, S., Hoess, P., Liu, S., et al. (2023) · Science · doi:10.1126/science.ade2676 · Cited by 167
Seeing beyond the limit: A guide to choosing the right super-resolution microscopy technique
Valli, J., Garcia-Burgos, A., Rooney, L. M., Vale de Melo e Oliveira, B., Duncan, R. R., & Rickman, C. (2021) · Journal of Biological Chemistry · doi:10.1016/j.jbc.2021.100791 · Cited by 159
One-step nanoscale expansion microscopy reveals individual protein shapes
Shaib, A. H., Chouaib, A. A., Chowdhury, R., Altendorf, J., Mihaylov, D., Zhang, C., Krah, D., Imani, V., Spencer, R. K. W., Georgiev, S. V., et al. (2025) · Nature Biotechnology · doi:10.1038/s41587-024-02431-9 · Cited by 104
MINSTED nanoscopy enters the Ångström localization range
Weber, M., von der Emde, H., Leutenegger, M., Gunkel, P., Sambandan, S., Khan, T. A., Keller-Findeisen, J., Cordes, V. C., & Hell, S. W. (2023) · Nature Biotechnology · doi:10.1038/s41587-022-01519-4 · Cited by 94
Self-citationImaging of anticancer drug action in single cells
Miller, M. A., & Weissleder, R. (2017) · Nature reviews. Cancer · doi:10.1038/nrc.2017.41 · Cited by 92
In situ genotyping of a pooled strain library after characterizing complex phenotypes
Lawson, M. J., Camsund, D., Larsson, J., Baltekin, Ö., Fange, D., & Elf, J. (2017) · Molecular Systems Biology · doi:10.15252/msb.20177951 · Cited by 82
Self-citationLive-cell imaging powered by computation
Shroff, H., Testa, I., Jug, F., & Manley, S. (2024) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-024-00702-6 · Cited by 75
Danial, J. S. H., Lam, J. Y. L., Wu, Y., Woolley, M., Dimou, E., Cheetham, M. R., Emin, D., & Klenerman, D. (2022) · Nature Protocols · doi:10.1038/s41596-022-00730-6 · Cited by 40
Verkest, C., Roettger, L., Zeitzschel, N., & Lechner, S. G. (2025) · Nature Communications · doi:10.1038/s41467-025-67610-x · Cited by 12
Integrative approaches to study enhancer–promoter communication
Mach, P., & Giorgetti, L. (2023) · Current Opinion in Genetics & Development · doi:10.1016/j.gde.2023.102052 · Cited by 10
Metadata
- Authors
- Balzarotti, F., Eilers, Y., Gwosch, K. C., Gynnå, A. H., Westphal, V., Stefani, F. D., Elf, J., & Hell, S. W.
- Venue
- Science
- Published
- 2017-02-10
- Volume
- 355
- Issue
- 6325
- Pages
- 606-612
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2554260837
- Cited by
- 1,225
- References
- 0
- FWCI
- 47.29
- Type
- journal article
- Language
- en
- Open access
- bronze
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