CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

Chen, Janice S., Ma, Enbo, Harrington, Lucas B., Da Costa, Maria, Tian, Xinran, Palefsky, Joel M., Doudna, Jennifer A.·2018·Science

DOI 10.1126/science.aar6245

Cited by (1,003)

Items 101–200 of 1,003

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Application of CRISPR-Cas12a temperature sensitivity for improved genome editing in rice, maize, and Arabidopsis
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High-throughput and all-solution phase African Swine Fever Virus (ASFV) detection using CRISPR-Cas12a and fluorescence based point-of-care system
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CRISPR Mediated Biosensing Toward Understanding Cellular Biology and Point‐of‐Care Diagnosis
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Glycerol Additive Boosts 100-fold Sensitivity Enhancement for One-Pot RPA-CRISPR/Cas12a Assay
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The CRISPR–Cas toolbox for analytical and diagnostic assay development
Yanan Tang, Lu Gao, Wei Feng, Guo Chen, Qianfan Yang, Feng Li, X. Chris Le · 2021 · Chemical Society Reviews
CRISPR/Cas12a-Mediated Interfacial Cleaving of Hairpin DNA Reporter for Electrochemical Nucleic Acid Sensing
Decai Zhang, Yurong Yan, Haiying Que, Tiantian Yang, Xiaoxue Cheng, Shijia Ding, Xiuming Zhang, Wei Cheng · 2020 · ACS Sensors
Point-of-care testing detection methods for COVID-19
Qi Song, Xindi Sun, Ziyi Dai, Yibo Gao, Xiuqing Gong, Bingpu Zhou, Jinbo Wu, Weijia Wen · 2021 · Lab on a Chip
One-tube SARS-CoV-2 detection platform based on RT-RPA and CRISPR/Cas12a
Yangyang Sun, Lei Yu, Chengxi Liu, Shanting Ye, Wei Chen, Dechang Li, Weiren Huang · 2021 · Journal of Translational Medicine
Amplification-free RNA detection with CRISPR–Cas13
Hajime Shinoda, Yuya Taguchi, Ryoya Nakagawa, Asami Makino, Sae Okazaki, Masahiro Nakano, Yukiko Muramoto, Chiharu Takahashi, Ikuko Takahashi, Jun Ando et al. · 2021 · Communications Biology
SHERLOCK and DETECTR: CRISPR-Cas Systems as Potential Rapid Diagnostic Tools for Emerging Infectious Diseases
Mujahed I. Mustafa, Abdelrafie Mohamed Makhawi · 2020 · Journal of Clinical Microbiology · Retracted
Virus Detection: A Review of the Current and Emerging Molecular and Immunological Methods
Arabelle Cassedy, Anne Parle‐McDermott, Richard J O’Kennedy · 2021 · Frontiers in Molecular Biosciences
Simplified Cas13-based assays for the fast identification of SARS-CoV-2 and its variants
Jon Arizti-Sanz, A'Doriann Y. Bradley, Yibin B. Zhang, Chloe K. Boehm, Catherine A. Freije, Michelle E. Grunberg, Tinna-Sólveig F. Kosoko-Thoroddsen, Nicole L. Welch, Priya P. Pillai, Sreekar Mantena et al. · 2022 · Nature Biomedical Engineering
Size-Controlled Engineering Photoelectrochemical Biosensor for Human Papillomavirus-16 Based on CRISPR-Cas12a-Induced Disassembly of Z-Scheme Heterojunctions
Yuxuan Li, Ruijin Zeng, Weijun Wang, Jianhui Xu, Hexiang Gong, Ling Li, Mei‐Jin Li, Dianping Tang · 2022 · ACS Sensors
CRISPR-based rapid and ultra-sensitive diagnostic test for Mycobacterium tuberculosis
Jingwen Ai, Xian Zhou, Teng Xu, Minling Yang, Yuanyuan Chen, Guiqing He, Ningp Pan, Yuwei Cai, Yongjun Li, Xiaorui Wang et al. · 2019 · Emerging Microbes & Infections
An engineered CRISPR-Cas12a variant and DNA-RNA hybrid guides enable robust and rapid COVID-19 testing
Kean Hean Ooi, Mengying Mandy Liu, Douglas Jie Wen Tay, Seok Yee Teo, Pornchai Kaewsapsak, Shengyang Jin, Chun Kiat Lee, Jingwen Hou, Sebastian Maurer‐Stroh, Weisi Lin et al. · 2021 · Nature Communications
Molecular Mechanisms of CRISPR-Cas Immunity in Bacteria
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CRISPR/Cas12a-Assisted Ligation-Initiated Loop-Mediated Isothermal Amplification (CAL-LAMP) for Highly Specific Detection of microRNAs
Mai Zhang, Honghong Wang, Hui Wang, Fangfang Wang, Zhengping Li · 2021 · Analytical Chemistry
Droplet Cas12a Assay Enables DNA Quantification from Unamplified Samples at the Single-Molecule Level
Huahua Yue, Bowen Shu, Tian Tian, Erhu Xiong, Mengqi Huang, Debin Zhu, Jian Ge Sun, Qing Liu, Shichan Wang, Yirong Li et al. · 2021 · Nano Letters
CRISPR-Cas-Based Antimicrobials: Design, Challenges, and Bacterial Mechanisms of Resistance
Arianna Mayorga-Ramos, Johana Zúñiga-Miranda, Saskya E. Carrera-Pacheco, Carlos Barba‐Ostria, Linda P. Guamán · 2023 · ACS Infectious Diseases
Split crRNA with CRISPR-Cas12a enabling highly sensitive and multiplexed detection of RNA and DNA
Yi-Chuan Chen, Xinping Wang, Junqi Zhang, Qingyuan Jiang, Bin Qiao, Baoxia He, Wenhao Yin, Jie Qiao, Yi Liu · 2024 · Nature Communications
Delivery of CRISPR/Cas systems for cancer gene therapy and immunotherapy
Xiangrong Song, Chao Liu, Ning Wang, Hai Huang, Siyan He, Changyang Gong, Yuquan Wei · 2020 · Advanced Drug Delivery Reviews
High-Fidelity and Rapid Quantification of miRNA Combining crRNA Programmability and CRISPR/Cas13a trans-Cleavage Activity
Yuanyue Shan, Xiaoming Zhou, Ru Qiang Huang, Da Xing · 2019 · Analytical Chemistry
CRISPR‐Cas Biochemistry and CRISPR‐Based Molecular Diagnostics
Zhengyan Weng, Zheng You, Jie Yang, Noor Mohammad, Mengshi Lin, Qingshan Wei, Xue Gao, Yi Zhang · 2023 · Angewandte Chemie International Edition
Rapid and sensitive detection of COVID-19 using CRISPR/Cas12a-based detection with naked eye readout, CRISPR/Cas12a-NER
Xinjie Wang, Mingtian Zhong, Liu Y, Peixiang Ma, Lu Dang, Qingzhou Meng, Wenwei Wan, Xiaodong Ma, Jia Liu, Guang Yang et al. · 2020 · 中国科学通报：英文版
Contamination-free visual detection of SARS-CoV-2 with CRISPR/Cas12a: A promising method in the point-of-care detection
Yanju Chen, Ya Shi, Yin Chen, Zhangnv Yang, Hui Wu, Zhihui Zhou, Jue Li, Jianfeng F Ping, Lu-Ping He, Hong Shen et al. · 2020 · Biosensors and Bioelectronics
Digital CRISPR/Cas‐Assisted Assay for Rapid and Sensitive Detection of SARS‐CoV‐2
Joon Soo Park, Kuangwen Hsieh, Liben Chen, Aniruddha M. Kaushik, Alexander Y. Trick, Tza‐Huei Wang · 2020 · Advanced Science
Molecular and Immunological Diagnostic Tests of COVID-19: Current Status and Challenges
Tuğba Kiliç, Ralph Weissleder, Hakho Lee · 2020 · iScience
CRISPR Cpf1 proteins: structure, function and implications for genome editing
Fatemeh Safari, Khadijeh Zare, Manica Negahdaripour, Mazyar Barekati‐Mowahed, Younes Ghasemi · 2019 · Cell & Bioscience
CRISPR/Cas12a-based photoelectrochemical sensing of microRNA on reduced graphene oxide-anchored Bi2WO6 coupling with catalytic hairpin assembly
Hexiang Gong, Xuehan Hu, Ruijin Zeng, Yuxuan Li, Jianhui Xu, Mei‐Jin Li, Dianping Tang · 2022 · Sensors and Actuators B Chemical
Systematic discovery of natural CRISPR-Cas12a inhibitors
Kyle E. Watters, Christof Fellmann, Hua Bai, Shawn M. Ren, Jennifer A. Doudna · 2018 · Science
A One-Pot Toolbox Based on Cas12a/crRNA Enables Rapid Foodborne Pathogen Detection at Attomolar Level
Yunqing Wang, Yuqing Ke, Wenjia Liu, Yiqing Sun, Xianting Ding · 2020 · ACS Sensors
RPA-Cas12a-FS: A frontline nucleic acid rapid detection system for food safety based on CRISPR-Cas12a combined with recombinase polymerase amplification
Hua Liu, Jinbin Wang, Haijuan Zeng, Xiaofeng Liu, Wei Jiang, Yu Wang, Wanbao Ouyang, Xueming Tang · 2020 · Food Chemistry
A one-pot CRISPR/Cas13a-based contamination-free biosensor for low-cost and rapid nucleic acid diagnostics
Fei Hu, Yanfei Liu, Shuhao Zhao, Zengming Zhang, Xichen Li, Niancai Peng, Zhuangde Jiang · 2022 · Biosensors and Bioelectronics
Novel non-nucleic acid targets detection strategies based on CRISPR/Cas toolboxes: A review
Xinkuan Cheng, Yaru Li, Jun Kou, Dan Liao, Wenlu Zhang, Lijuan Yin, Shuli Man, Long Ma · 2022 · Biosensors and Bioelectronics
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Xianxian Zhao, Wenqing Zhang, Xiaopei Qiu, Qiang Mei, Yang Luo, Weiling Fu · 2020 · Analytical and Bioanalytical Chemistry
Surpassing the detection limit and accuracy of the electrochemical DNA sensor through the application of CRISPR Cas systems
Wei Xu, Tian Jin, Yifan Dai, Chung-Chiun Liu · 2020 · Biosensors and Bioelectronics
Short prokaryotic Argonaute systems trigger cell death upon detection of invading DNA
Balwina Koopal, Ana Potocnik, Sumanth Kumar Mutte, Cristian Aparicio-Maldonado, Simon Lindhoud, Jacques J. M. Vervoort, Stan J. J. Brouns, Daan C. Swarts · 2022 · Cell
Photoactivatable CRISPR/Cas12a Strategy for One-Pot DETECTR Molecular Diagnosis
Yong Chen, Xiaoling Xu, Jiachun Wang, Yibin Zhang, Wentao Zeng, Yizhen Liu, Xueji Zhang · 2022 · Analytical Chemistry
Structural biology of CRISPR–Cas immunity and genome editing enzymes
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Asymmetric CRISPR enabling cascade signal amplification for nucleic acid detection by competitive crRNA
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Analysis of short tandem repeat expansions and their methylation state with nanopore sequencing
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CRISPR-Cas systems for diagnosing infectious diseases
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CRISPR-Cas12a-Powered Dual-Mode Biosensor for Ultrasensitive and Cross-validating Detection of Pathogenic Bacteria
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Efficient, Rapid, and Sensitive Detection of Plant RNA Viruses With One-Pot RT-RPA–CRISPR/Cas12a Assay
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CRISPR/Cas12a-based technology: A powerful tool for biosensing in food safety
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Applying CRISPR-Cas12a as a Signal Amplifier to Construct Biosensors for Non-DNA Targets in Ultralow Concentrations
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CRISPR–Cas: a tool for cancer research and therapeutics
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CRISPR Enzyme Kinetics for Molecular Diagnostics
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Prashanth Ramachandran, Michael R. Wilson · 2020 · Nature Reviews Neurology
Exploring the Trans-Cleavage Activity of CRISPR/Cas12a on Gold Nanoparticles for Stable and Sensitive Biosensing
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Naked-Eye Detection of Grapevine Red-Blotch Viral Infection Using a Plasmonic CRISPR Cas12a Assay
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Light‐Start CRISPR‐Cas12a Reaction with Caged crRNA Enables Rapid and Sensitive Nucleic Acid Detection
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Enzyme Kinetics and Detector Sensitivity Determine Limits of Detection of Amplification-Free CRISPR-Cas12 and CRISPR-Cas13 Diagnostics
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CRISPR-Cas-amplified urinary biomarkers for multiplexed and portable cancer diagnostics
Liangliang Hao, Renee Tan Zhao, Nicole L. Welch, Edward K.W. Tan, Qian Zhong, Nour Saida Harzallah, Chayanon Ngambenjawong, Henry Ko, Heather E. Fleming, Pardis Christine Sabeti et al. · 2023 · Nature Nanotechnology
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Enhancing CRISPR-Cas-Mediated Detection of Nucleic Acid and Non-nucleic Acid Targets Using Enzyme-Labeled Reporters
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Argonaute integrated single-tube PCR system enables supersensitive detection of rare mutations
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A CRISPR-based and post-amplification coupled SARS-CoV-2 detection with a portable evanescent wave biosensor
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CRISPR-Cas12a enhanced rolling circle amplification method for ultrasensitive miRNA detection
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Clustered Regularly Interspaced Short Palindromic Repeats-Mediated Surface-Enhanced Raman Scattering Assay for Multidrug-Resistant Bacteria
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CRISPR-Cas14 is now part of the artillery for gene editing and molecular diagnostic
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CRISPR-Cas12a Coupled with Platinum Nanoreporter for Visual Quantification of SNVs on a Volumetric Bar-Chart Chip
Ning Shao, Xin Han, Yanni Song, Pengchao Zhang, Lidong Qin · 2019 · Analytical Chemistry
MeCas12a, a Highly Sensitive and Specific System for COVID‐19 Detection
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A CRISPR/Cas13a-powered catalytic electrochemical biosensor for successive and highly sensitive RNA diagnostics
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Noncanonical crRNAs derived from host transcripts enable multiplexable RNA detection by Cas9
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Signal Amplification by the trans -Cleavage Activity of CRISPR-Cas Systems: Kinetics and Performance
Wei Feng, Hongquan Zhang, X. Chris Le · 2023 · Analytical Chemistry
Assessment of Cas12a‐mediated gene editing efficiency in plants
Joan Miquel Bernabé‐Orts, Iván Casas‐Rodrigo, Eugenio G. Minguet, Viola Landolfi, Victor García‐Carpintero, Silvia Gianoglio, Marta Vázquez‐Vilar, Antonio Granell, Diego Orzáez · 2019 · Plant Biotechnology Journal
Cas12a-Based On-Site and Rapid Nucleic Acid Detection of African Swine Fever
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CRISPR-Cas12a has widespread off-target and dsDNA-nicking effects
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