CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

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Items 201–300 of 1,003

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2. An Update on Molecular Diagnostics for COVID-19
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3. An amplification-free ultra-sensitive electrochemical CRISPR/Cas biosensor for drug-resistant bacteria detection
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4. A novel fluorescence biosensor based on CRISPR/Cas12a integrated MXenes for detecting Aflatoxin B1
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5. An ultrasensitive, rapid, and portable coronavirus SARS-CoV-2 sequence detection method based on CRISPR-Cas12
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6. Highly specific enrichment of rare nucleic acid fractions using Thermus thermophilus argonaute with applications in cancer diagnostics
   Jinzhao Song, Jorrit W. Hegge, Michael G. Mauk, Junman Chen, Jacob Edward Till, Neha Bhagwat, Lotte T. Azink, Jing Yun Peng, Moen Sen, Jazmine J. Mays et al. · 2019 · Nucleic Acids Research
7. Improved LbCas12a variants with altered PAM specificities further broaden the genome targeting range of Cas12a nucleases
   Eszter Tóth, Éva Varga, Péter István Kulcsár, Virág Kocsis-Jutka, Sarah Laura Krausz, Antal Nyeste, Zsombor Welker, Krisztina Huszár, Zoltán Ligeti, András Tálas et al. · 2020 · Nucleic Acids Research
8. CRISPR-cas12a mediated SERS lateral flow assay for amplification-free detection of double-stranded DNA and single-base mutation
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9. Rapid detection of SARS-CoV-2 RNA in saliva via Cas13
   Sita S. Chandrasekaran, Shreeya Agrawal, Alison Fanton, Aditya R. Jangid, Bérénice Charrez, Arturo M. Escajeda, Sungmin Son, Roger McIntosh, Huyen Tran, Abdul Muyeed Bhuiya et al. · 2022 · Nature Biomedical Engineering
10. Principles and Applications of Loop-Mediated Isothermal Amplification to Point-of-Care Tests
    Jee-Woong Park · 2022 · Biosensors
11. An ultrasensitive hybridization chain reaction-amplified CRISPR-Cas12a aptasensor for extracellular vesicle surface protein quantification
    Shan Xing, Zedong Lu, Qi Huang, Huilan Li, Yu Wang, Yanzhen Lai, Yi He, Min Deng, Wanli Liu · 2020 · Theranostics
12. CRISPR-Cas Biology and Its Application to Infectious Diseases
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13. Towards application of CRISPR-Cas12a in the design of modern viral DNA detection tools (Review)
    Julija Dronina, Urtė Samukaitė-Bubnienė, Arūnas Ramanavičius · 2022 · Journal of Nanobiotechnology
14. COVID‐19 Clinical Diagnostics and Testing Technology
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15. CRISPR-based detection of SARS-CoV-2: A review from sample to result
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16. Switching the activity of Cas12a using guide RNA strand displacement circuits
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17. Universal and high-fidelity DNA single nucleotide polymorphism detection based on a CRISPR/Cas12a biochip
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18. CRISPR/Cas-powered nanobiosensors for diagnostics
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19. Recent advances and applications of CRISPR-Cas9 in cancer immunotherapy
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20. Signal amplification and output of CRISPR/Cas-based biosensing systems: A review
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21. Rapid Detection of 2019 Novel Coronavirus SARS-CoV-2 Using a CRISPR-based DETECTR Lateral Flow Assay
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22. Cascade CRISPR/cas enables amplification-free microRNA sensing with fM-sensitivity and single-base-specificity
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23. Construct design for CRISPR/Cas-based genome editing in plants
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24. CRISPR-Based Diagnosis of Infectious and Noninfectious Diseases
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25. CRISPR Systems for COVID-19 Diagnosis
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26. Lateral Flow Assay: A Summary of Recent Progress for Improving Assay Performance
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27. Selective endpoint visualized detection of Vibrio parahaemolyticus with CRISPR/Cas12a assisted PCR using thermal cycler for on-site application
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28. CRISPR-Cas12a based aptasensor for sensitive and selective ATP detection
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29. Sensitive quantitative detection of SARS-CoV-2 in clinical samples using digital warm-start CRISPR assay
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30. Strand Displacement Amplification Assisted CRISPR-Cas12a Strategy for Colorimetric Analysis of Viral Nucleic Acid
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31. Massively parallel kinetic profiling of natural and engineered CRISPR nucleases
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32. Integrating Reverse Transcription Recombinase Polymerase Amplification with CRISPR Technology for the One-Tube Assay of RNA
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33. The CRISPR/Cas revolution continues: From efficient gene editing for crop breeding to plant synthetic biology
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34. The application of CRISPR‐Cas for single species identification from environmental DNA
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35. Synthetic biology in the clinic: engineering vaccines, diagnostics, and therapeutics
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36. Exploring the Trans‐Cleavage Activity of CRISPR‐Cas12a (cpf1) for the Development of a Universal Electrochemical Biosensor
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37. Fast and sensitive CRISPR detection by minimized interference of target amplification
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38. Versatile detection with CRISPR/Cas system from applications to challenges
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39. Rapid detection of SARS-CoV-2 with CRISPR-Cas12a
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40. LAMP-Coupled CRISPR–Cas12a Module for Rapid and Sensitive Detection of Plant DNA Viruses
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41. Recent developments and future directions in point-of-care next-generation CRISPR-based rapid diagnosis
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42. Enhancing the analytical performance of paper lateral flow assays: From chemistry to engineering
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43. MXene Coupled with CRISPR-Cas12a for Analysis of Endotoxin and Bacteria
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44. Kinetic analysis of Cas12a and Cas13a RNA-Guided nucleases for development of improved CRISPR-Based diagnostics
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45. Dual-CRISPR/Cas12a-Assisted RT-RAA for Ultrasensitive SARS-CoV-2 Detection on Automated Centrifugal Microfluidics
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46. A high fidelity CRISPR/Cas12a based lateral flow biosensor for the detection of HPV16 and HPV18
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47. CRISPR/Cas12a based fluorescence-enhanced lateral flow biosensor for detection of Staphylococcus aureus
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48. New CRISPR-Derived microRNA Sensing Mechanism Based on Cas12a Self-Powered and Rolling Circle Transcription-Unleashed Real-Time crRNA Recruiting
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49. Sequence-Specific Recognition of HIV-1 DNA with Solid-State CRISPR-Cas12a-Assisted Nanopores (SCAN)
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50. Aptamer assisted CRISPR-Cas12a strategy for small molecule diagnostics
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51. CRISPR-Cas12a-mediated luminescence resonance energy transfer aptasensing platform for deoxynivalenol using gold nanoparticle-decorated Ti3C2Tx MXene as the enhanced quencher
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52. A Cas9 with PAM recognition for adenine dinucleotides
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53. Optimized design parameters for CRISPR Cas9 and Cas12a homology-directed repair
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54. Label-Free Colorimetric Method for Detection of Vibrio parahaemolyticus by Trimming the G-Quadruplex DNAzyme with CRISPR/Cas12a
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55. Argonaute-integrated isothermal amplification for rapid, portable, multiplex detection of SARS-CoV-2 and influenza viruses
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56. Advances in biosensing: The CRISPR/Cas system as a new powerful tool for the detection of nucleic acids
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57. Genome editing for plant research and crop improvement
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58. Sensitive and rapid on-site detection of SARS-CoV-2 using a gold nanoparticle-based high-throughput platform coupled with CRISPR/Cas12-assisted RT-LAMP
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59. Evaluation of CRISPR/Cas12a-based DNA detection for fast pathogen diagnosis and GMO test in rice
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60. Applications of CRISPR-Cas Enzymes in Cancer Therapeutics and Detection
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61. Structure and mechanism of a Type III CRISPR defence DNA nuclease activated by cyclic oligoadenylate
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62. A CRISPR-based assay for the detection of opportunistic infections post-transplantation and for the monitoring of transplant rejection
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63. Holographic Optical Tweezers and Boosting Upconversion Luminescent Resonance Energy Transfer Combined Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas12a Biosensors
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64. Designing sensitive viral diagnostics with machine learning
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65. Broad-spectrum enzymatic inhibition of CRISPR-Cas12a
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66. Allosteric Probe-Initiated Wash-Free Method for Sensitive Extracellular Vesicle Detection through Dual Cycle-Assisted CRISPR-Cas12a
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67. Duplex-Specific Nuclease-Assisted CRISPR-Cas12a Strategy for MicroRNA Detection Using a Personal Glucose Meter
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68. A Comprehensive Review of Detection Methods for SARS-CoV-2
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69. Emerging biosensing and transducing techniques for potential applications in point-of-care diagnostics
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70. CRISPR-Cas12a-Empowered Electrochemical Biosensor for Rapid and Ultrasensitive Detection of SARS-CoV-2 Delta Variant
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71. An RNA‐Guided Cas9 Nickase‐Based Method for Universal Isothermal DNA Amplification
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72. CRISPR-Cas12a/Cpf1-assisted precise, efficient and multiplexed genome-editing in Yarrowia lipolytica
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73. Miniature type V-F CRISPR-Cas nucleases enable targeted DNA modification in cells
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74. DropCRISPR: A LAMP-Cas12a based digital method for ultrasensitive detection of nucleic acid
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75. Ultrasensitive DNA Origami Plasmon Sensor for Accurate Detection in Circulating Tumor DNAs
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76. Rapid detection of African swine fever virus using Cas12a-based portable paper diagnostics
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77. Advances in Cell‐Free Biosensors: Principle, Mechanism, and Applications
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78. Digital CRISPR-based method for the rapid detection and absolute quantification of nucleic acids
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81. Research progress on detection of foodborne pathogens: The more rapid and accurate answer to food safety
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82. Cell-Free Gene Expression: Methods and Applications
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83. Emerging ultrafast nucleic acid amplification technologies for next-generation molecular diagnostics
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84. End-point dual specific detection of nucleic acids using CRISPR/Cas12a based portable biosensor
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85. Rapid, Ultrasensitive, and Highly Specific Diagnosis of COVID-19 by CRISPR-Based Detection
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86. Advances and insights in the diagnosis of viral infections
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87. PAM-free loop-mediated isothermal amplification coupled with CRISPR/Cas12a cleavage (Cas-PfLAMP) for rapid detection of rice pathogens
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88. Visual detection for nucleic acid-based techniques as potential on-site detection methods. A review
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90. Two-Dimensional Material-Based Biosensors for Virus Detection
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92. Electrochemiluminescence covalent organic framework coupling with CRISPR/Cas12a-mediated biosensor for pesticide residue detection
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93. Tunable control of Cas12 activity promotes universal and fast one-pot nucleic acid detection
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94. CRISPR/Cas12a-Derived electrochemical aptasensor for ultrasensitive detection of COVID-19 nucleocapsid protein
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95. Trends in developing one-pot CRISPR diagnostics strategies
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96. Genome Editing Technologies for Rice Improvement: Progress, Prospects, and Safety Concerns
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99. Recent advancements in nucleic acid detection with microfluidic chip for molecular diagnostics
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100. Digital Recombinase Polymerase Amplification, Digital Loop‐Mediated Isothermal Amplification, and Digital CRISPR‐Cas Assisted Assay: Current Status, Challenges, and Perspectives
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