CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

Chen, Janice S., Ma, Enbo, Harrington, Lucas B., Da Costa, Maria, Tian, Xinran, Palefsky, Joel M., Doudna, Jennifer A.·2018·Science

DOI 10.1126/science.aar6245

Cited by (1,003)

Items 301–400 of 1,003

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Long Thành Nguyễn, Nicolas C. Macaluso, Brianna L.M. Pizzano, Melanie N. Cash, Jan Špaček, Jan Karásek, Megan R. Miller, John A. Lednicky, Rhoel R. Dinglasan, Marco Salemi et al. · 2022 · EBioMedicine
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Vishakha Vashisht, Ashutosh Vashisht, Ashis Kumar Mondal, Jaspreet Kaur Farmaha, Ahmet Alptekin, Harmanpreet Singh, Pankaj Kumar Ahluwalia, Anaka Srinivas, Ravindra B Kolhe · 2023 · BioMedInformatics
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CRISPR-/Cas12a-Mediated Liposome-Amplified Strategy for the Surface-Enhanced Raman Scattering and Naked-Eye Detection of Nucleic Acid and Application to Food Authenticity Screening
Jianghua Liu, Jiahui Chen, Di Wu, Mingquan Huang, Jian Chen, Ruiyuan Pan, Yongning Wu, Guoliang Li · 2021 · Analytical Chemistry
AND Logic-Gate-Based CRISPR/Cas12a Biosensing Platform for the Sensitive Colorimetric Detection of Dual miRNAs
Shaohua Gong, Xi Wang, Ping Zhou, Wei Pan, Na Li, Bo Tang · 2022 · Analytical Chemistry
TnpB structure reveals minimal functional core of Cas12 nuclease family
Giedrius Sasnauskas, Giedrė Tamulaitienė, Gytis Druteika, Arturo Carabias, Arūnas Šilanskas, Darius Kazlauskas, Česlovas Venclovas, Guillermo Montoya, Tautvydas Karvelis, Virginijus Šikšnys · 2023 · Nature
CRISPR diagnostics
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Class 2 CRISPR/Cas: an expanding biotechnology toolbox for and beyond genome editing
Yuyi Tang, Yan Fu · 2018 · Cell & Bioscience
Ultrasensitive electrochemical assay for microRNA-21 based on CRISPR/Cas13a-assisted catalytic hairpin assembly
Ying Cui, Shanji Fan, Ze Yuan, Minghui Song, Jiawen Hu, Dong Jin Qian, Deshuai Zhen, Junhua Li, Baode Zhu · 2020 · Talanta
TB-QUICK: CRISPR-Cas12b-assisted rapid and sensitive detection of Mycobacterium tuberculosis
Ikuan Sam, Yingying Chen, Jun Ma, Shiyuan Li, Ruoyan Ying, Lin-xian Li, Ping Ji, Shujun Wang, Jie Xu, Yu-Jie Bao et al. · 2021 · Journal of Infection
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Kazuto Yoshimi, Kohei Takeshita, Seiya Yamayoshi, Satomi Shibumura, Yuko Yamauchi, Masaki Yamamoto, Hiroshi Yotsuyanagi, Yoshihiro Kawaoka, Tomoji Mashimo · 2022 · iScience
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Aijiao Yuan, Rui Sha, Wenjing Xie, Guangbo Qu, Hongquan Zhang, Hailin Wang, X. Chris Le, Guibin Jiang, Hanyong Peng · 2024 · Journal of the American Chemical Society
Coevolution between bacterial CRISPR-Cas systems and their bacteriophages
Bridget N. J. Watson, Jurre A. Steens, Raymond H.J. Staals, Edze Rients Westra, Stineke van Houte · 2021 · Cell Host & Microbe
DNA interference states of the hypercompact CRISPR–CasΦ effector
Patrick Pausch, Katarzyna M. Soczek, Dominik A. Herbst, Connor A. Tsuchida, Basem Al-Shayeb, Jillian F. Banfield, Eva Nogales, Jennifer A. Doudna · 2021 · Nature Structural & Molecular Biology
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Alangar Ishwara Bhat, Rashid Abdi Aman, Magdy M. Mahfouz · 2022 · Plant Biotechnology Journal
Diagnostics of Infections Produced by the Plant Viruses TMV, TEV, and PVX with CRISPR-Cas12 and CRISPR-Cas13
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A SERS-signalled, CRISPR/Cas-powered bioassay for amplification-free and anti-interference detection of SARS-CoV-2 in foods and environmental samples using a single tube-in-tube vessel
Long Ma, Wenlu Zhang, Lijuan Yin, Yaru Li, Jianwen Zhuang, Liang Shen, Shuli Man · 2023 · Journal of Hazardous Materials
Modular one-pot assembly of CRISPR arrays enables library generation and reveals factors influencing crRNA biogenesis
Chunyu Liao, Fani Ttofali, Rebecca Slotkowski, Steven R. Denny, Taylor D. Cecil, Ryan T. Leenay, Albert J. Keung, Chase L. Beisel · 2019 · Nature Communications
A CRISPR Test for Detection of Circulating Nuclei Acids
Jen-Hui Tsou, Qixin Leng, Feng Jiang · 2019 · Translational Oncology
A versatile biosensing platform coupling CRISPR–Cas12a and aptamers for detection of diverse analytes
Xiangxiang Zhao, Shanshan Li, Guang Liu, Zhong Wang, Zhiheng Yang, Quanwei Zhang, Mindong Liang, Jiakun Liu, Zilong Li, Yaojun Tong et al. · 2020 · 中国科学通报：英文版
Colorimetric Detection of SARS-CoV-2 and Drug-Resistant pH1N1 Using CRISPR/dCas9
Jeong Moon, Hyung-Jun Kwon, Dongeun Yong, In-Chul Lee, Hongki Kim, Hyunju Kang, Eun‐Kyung Lim, Kyu‐Sun Lee, Juyeon Jung, Hyun Gyu Park et al. · 2020 · ACS Sensors
Dual Methylation-Sensitive Restriction Endonucleases Coupling with an RPA-Assisted CRISPR/Cas13a System (DESCS) for Highly Sensitive Analysis of DNA Methylation and Its Application for Point-of-Care Detection
Xianfeng Wang, Shiying Zhou, Chengxiang Chu, Mei Jun Yang, Danqun Huo, Changjun Hou · 2021 · ACS Sensors
Point-of-care CRISPR-Cas-assisted SARS-CoV-2 detection in an automated and portable droplet magnetofluidic device
Fan‐En Chen, Pei‐Wei Lee, Alexander Y. Trick, Joon Soo Park, Liben Chen, Kushagra Shah, Heba H. Mostafa, Karen C. Carroll, Kuangwen Hsieh, Tza‐Huei Wang · 2021 · Biosensors and Bioelectronics
A novel CRISPR-based malaria diagnostic capable of Plasmodium detection, species differentiation, and drug-resistance genotyping
Clark H. Cunningham, Christopher M. Hennelly, Jessica T. Lin, Ratawan Ubalee, Ross M. Boyce, Edgar Mugema Mulogo, Nicholas J. Hathaway, Kyaw L. Thwai, Fernandine Phanzu, Albert Ntumba Kalonji et al. · 2021 · EBioMedicine
The miniature CRISPR-Cas12m effector binds DNA to block transcription
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Potential application of CRISPR-Cas12a fluorescence assay coupled with rapid nucleic acid amplification for detection of white spot syndrome virus in shrimp
Thawatchai Chaijarasphong, Thanyawit Thammachai, Ornchuma Itsathitphaisarn, Kallaya Sritunyalucksana, Rungkarn Suebsing · 2019 · Aquaculture
CRISPR/Cas12a-mediated gold nanoparticle aggregation for colorimetric detection of SARS-CoV-2
Yiren Cao, Jinjun Wu, Bo Pang, Hongquan Zhang, X. Chris Le · 2021 · Chemical Communications
Amplification‐Free Detection of SARS‐CoV‐2 and Respiratory Syncytial Virus Using CRISPR Cas13a and Graphene Field‐Effect Transistors
Huijie Li, Jie Yang, Guangfu Wu, Zhengyan Weng, Yang Song, Yuxuan Zhang, Jeffrey A. Vanegas, Lori Avery, Zan Jun Gao, He Sun et al. · 2022 · Angewandte Chemie International Edition
Multiplexed lateral flow assay integrated with orthogonal CRISPR-Cas system for SARS-CoV-2 detection
Gaoxing Su, Min Zhu, Diyuan Li, Mengting Xu, Yuedong Zhu, Yan Zhang, Hongyan Zhu, Li Feng, Yanyan Yu · 2022 · Sensors and Actuators B Chemical
Transforming plant biology and breeding with CRISPR /Cas9, Cas12 and Cas13
Patrick Schindele, Felix Wolter, Holger Puchta · 2018 · FEBS Letters
Pathogen detection in the CRISPR–Cas era
Dipali G. Sashital · 2018 · Genome Medicine
CRISPR/Cas12-Based Ultra-Sensitive and Specific Point-of-Care Detection of HBV
Ronghua Ding, Jinzhao Long, Mingzhu Yuan, Xue Yan Zheng, Yue Shen, Yuefei Jin, Haiyan Yang, Hao Li, Shuaiyin Chen, Guangcai Duan · 2021 · International Journal of Molecular Sciences
CRISPR/Cas-engineered technology: Innovative approach for biosensor development
Taraneh Sadat Zavvar, Zahra Khoshbin, Mohammad Ramezani, Mona Alibolandi, Khalil Abnous, Seyed Mohammad Taghdisi · 2022 · Biosensors and Bioelectronics
A CRISPR-Cas12a integrated SERS nanoplatform with chimeric DNA/RNA hairpin guide for ultrasensitive nucleic acid detection
Bohan Yin, Qin Zhang, Xinyue Xia, Chuanqi Li, Willis Kwun Hei Ho, Jiaxiang Yan, Yingying Huang, Honglian Wu, Pui Wang, Changqing Yi et al. · 2022 · Theranostics
Universal crRNA Acylation Strategy for Robust Photo‐Initiated One‐Pot CRISPR–Cas12a Nucleic Acid Diagnostics
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Uracil-Mediated New Photospacer-Adjacent Motif of Cas12a To Realize Visualized DNA Detection at the Single-Copy Level Free from Contamination
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Bio-SCAN: A CRISPR/dCas9-Based Lateral Flow Assay for Rapid, Specific, and Sensitive Detection of SARS-CoV-2
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Structural Basis for the Inhibition of CRISPR-Cas12a by Anti-CRISPR Proteins
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CRISPR/Cas Multiplexed Biosensing: A Challenge or an Insurmountable Obstacle?
Yi Li, Linyang Liu, Guozhen Liu · 2019 · Trends in biotechnology
High-Fidelity CRISPR/Cas13a trans-Cleavage-Triggered Rolling Circle Amplified DNAzyme for Visual Profiling of MicroRNA
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Electric field-enhanced electrochemical CRISPR biosensor for DNA detection
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CRISPR/Cas-Based In Vitro Diagnostic Platforms for Cancer Biomarker Detection
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Yidan Tang, Lijuan Qi, Yichen Liu, Lulu Guo, Rujian Zhao, Meiting Yang, Yan Du, Bingling Li · 2022 · Angewandte Chemie International Edition
Paper Device Combining CRISPR/Cas12a and Reverse-Transcription Loop-Mediated Isothermal Amplification for SARS-CoV-2 Detection in Wastewater
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The development of a fluorescence/colorimetric biosensor based on the cleavage activity of CRISPR-Cas12a for the detection of non-nucleic acid targets
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CRISPR-Based COVID-19 Testing: Toward Next-Generation Point-of-Care Diagnostics
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LAMP-CRISPR-Cas12-based diagnostic platform for detection of Mycobacterium tuberculosis complex using real-time fluorescence or lateral flow test
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Harnessing Multiplex crRNA in the CRISPR/Cas12a System Enables an Amplification-Free DNA Diagnostic Platform for ASFV Detection
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Machine Learning-Assisted, Dual-Channel CRISPR/Cas12a Biosensor-In-Microdroplet for Amplification-Free Nucleic Acid Detection for Food Authenticity Testing
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