Cited by 4,507CRISPR and Genetic EngineeringAdvanced biosensing and bioanalysis techniquesRNA regulation and disease

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CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

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Chen, Janice S., Ma, Enbo, Harrington, Lucas B., Da Costa, Maria, Tian, Xinran, Palefsky, Joel M., Doudna, Jennifer A.·2018·Science

DOI 10.1126/science.aar6245PMC PMC6628903

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Abstract

CRISPR-Cas12a (Cpf1) proteins are RNA-guided enzymes that bind and cut DNA as components of bacterial adaptive immune systems. Like CRISPR-Cas9, Cas12a has been harnessed for genome editing on the basis of its ability to generate targeted, double-stranded DNA breaks. Here we show that RNA-guided DNA binding unleashes indiscriminate single-stranded DNA (ssDNA) cleavage activity by Cas12a that completely degrades ssDNA molecules. We find that target-activated, nonspecific single-stranded deoxyribonuclease (ssDNase) cleavage is also a property of other type V CRISPR-Cas12 enzymes. By combining Cas12a ssDNase activation with isothermal amplification, we create a method termed DNA endonuclease-targeted CRISPR trans reporter (DETECTR), which achieves attomolar sensitivity for DNA detection. DETECTR enables rapid and specific detection of human papillomavirus in patient samples, thereby providing a simple platform for molecular diagnostics.

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A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012) · Science · doi:10.1126/science.1225829 · Cited by 17,116
Self-citation
The new frontier of genome engineering with CRISPR-Cas9
Doudna, J. A., & Charpentier, E. (2014) · Science · doi:10.1126/science.1258096 · Cited by 7,062
Self-citation
CRISPR Provides Acquired Resistance Against Viruses in Prokaryotes
Barrangou, R., Fremaux, C., Deveau, H., Richards, M., Boyaval, P., Moineau, S., Romero, D. A., & Horvath, P. (2007) · Science · doi:10.1126/science.1138140 · Cited by 5,947
Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System
Zetsche, B., Gootenberg, J. S., Abudayyeh, O. O., Slaymaker, I. M., Makarova, K. S., Essletzbichler, P., Volz, S. E., Joung, J., van der Oost, J., Regev, A., et al. (2015) · Cell · doi:10.1016/j.cell.2015.09.038 · Cited by 4,761
Nucleic acid detection with CRISPR-Cas13a/C2c2
Gootenberg, J. S., Abudayyeh, O. O., Lee, J. W., Essletzbichler, P., Dy, A. J., Joung, J., Verdine, V. K., Donghia, N. M., Daringer, N. M., Freije, C. A., et al. (2017) · Science · doi:10.1126/science.aam9321 · Cited by 3,887
An updated evolutionary classification of CRISPR–Cas systems
Makarova, K. S., Wolf, Y. I., Alkhnbashi, O. S., Costa, F., Shah, S. A., Saunders, S. J., Barrangou, R., Brouns, S. J. J., Charpentier, E., Haft, D. H., et al. (2015) · Nature Reviews Microbiology · doi:10.1038/nrmicro3569 · Cited by 2,480
C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector
Abudayyeh, O. O., Gootenberg, J. S., Konermann, S., Joung, J., Slaymaker, I. M., Cox, D. B. T., Shmakov, S., Makarova, K. S., Semenova, E., Minakhin, L., et al. (2016) · Science · doi:10.1126/science.aaf5573 · Cited by 2,336
Crystal structure of Cas9 in complex with guide RNA and target DNA
Nishimasu, H., Ran, F. A., Hsu, P. D., Konermann, S., Shehata, S. I., Dohmae, N., Ishitani, R., Zhang, F., & Nureki, O. (2014) · Cell · doi:10.1016/j.cell.2014.02.001 · Cited by 2,306
DNA interrogation by the CRISPR RNA-guided endonuclease Cas9
Sternberg, S. H., Redding, S., Jinek, M., Greene, E. C., & Doudna, J. A. (2014) · Nature · doi:10.1038/nature13011 · Cited by 1,822
Self-citation
CRISPR Interference Limits Horizontal Gene Transfer in Staphylococci by Targeting DNA
Marraffini, L. A., & Sontheimer, E. J. (2008) · Science · doi:10.1126/science.1165771 · Cited by 1,642

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Tanny, T., Sallam, M., Soda, N., Nguyen, N., Alam, M. M., & Shiddiky, M. J. A. (2023) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.3c00913 · Cited by 47
DNAzyme-based faithful probing and pulldown to identify candidate biomarkers of low abundance
Hu, Q., Tong, Z.-X., Yalikong, A., Ge, L., Shi, Q., Du, X., Wang, P., Liu, X., Zhan, W., Gao, X., et al. (2023) · Nature Chemistry · doi:10.1038/s41557-023-01328-5 · Cited by 50
Next-generation CRISPR-based diagnostic tools for human diseases
Wang, T., Wang, Z., Bai, L., Zhang, X., Feng, J., Qian, C., Wang, Y., & Wang, R. (2023) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2023.117328 · Cited by 46
CRISPR-Responsive RCA-Based DNA Hydrogel Biosensing Platform with Customizable Signal Output for Rapid and Sensitive Nucleic Acid Detection
Zhang, Y., Wang, W., Zhou, X., Lin, H., Zhu, X., Lou, Y., & Zheng, L. (2024) · Analytical Chemistry · doi:10.1021/acs.analchem.4c03450 · Cited by 45
Proximity-activated guide RNA of CRISPR–Cas12a for programmable diagnostic detection and gene regulation
Hu, Z., Ling, S., Duan, J., Yu, Z., Che, Y., Wang, S., Zhang, S., Zhang, X., & Li, Z. (2025) · Nucleic Acids Research · doi:10.1093/nar/gkaf017 · Cited by 46
CRISPR/Cas12a mediated knock-in of the Polled Celtic variant to produce a polled genotype in dairy cattle
Schuster, F., Aldag, P., Frenzel, A., Hadeler, K., Lucas‐Hahn, A., Niemann, H., & Petersen, B. (2020) · Scientific Reports · doi:10.1038/s41598-020-70531-y · Cited by 50
Potential applications of CRISPR/Cas for next-generation biomonitoring of harmful algae blooms: A review
Durán‐Vinet, B., Araya‐Castro, K., Chao, T., Wood, S., Gallardo, V., Godoy, K., & Abanto, M. (2021) · Harmful Algae · doi:10.1016/j.hal.2021.102027 · Cited by 45
Detection of rare variant alleles using the AsCas12a double-stranded DNA trans-cleavage activity
Yu, H. L. L., Cao, Y., Lu, X., & Hsing, I. (2021) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2021.113382 · Cited by 45
Sensitive and Rapid Detection of Citrus Scab Using an RPA-CRISPR/Cas12a System Combined with a Lateral Flow Assay
Shin, K., Kwon, S.-H., Lee, S.-C., & Moon, Y. E. (2021) · Plants · doi:10.3390/plants10102132 · Cited by 45
CRISPR-Cas12a-Based Diagnostics of Wheat Fungal Diseases
Mu, K., Ren, X., Yang, H., Zhang, T., Yan, W., Yuan, F., Wu, J., Kang, Z., Han, D., Deng, R., et al. (2022) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.1c08391 · Cited by 45
Current status, advances, challenges and perspectives on biosensors for COVID-19 diagnosis in resource-limited settings
Shen, Y. H., Anwar, T. B., & Mulchandani, A. (2021) · Sensors and Actuators Reports · doi:10.1016/j.snr.2021.100025 · Cited by 44
Cas12a variants designed for lower genome-wide off-target effect through stringent PAM recognition
Zhou, J., Chen, P., Wang, H., Liu, H., Li, Y., Zhang, Y., Wu, Y., Paek, C., Sun, Z., Lei, J., et al. (2021) · Molecular Therapy · doi:10.1016/j.ymthe.2021.10.010 · Cited by 44
COVID-19 Variant Detection with a High-Fidelity CRISPR-Cas12 Enzyme
Fasching, C. L., Servellita, V., McKay, B., Nagesh, V., Broughton, J. P., Sotomayor-González, A., Wang, B., Brazer, N., Reyes, K. R., Streithorst, J. A., et al. (2022) · Journal of Clinical Microbiology · doi:10.1128/jcm.00261-22 · Cited by 44
Self-citation
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Rapid and sensitive detection of two fungal pathogens in soybeans using the recombinase polymerase amplification/ CRISPR‐Cas12a method for potential on‐site disease diagnosis
Sun, X., Lei, R., Zhang, H., Chen, W., Jia, Q., Guo, X., Zhang, Y., Wu, P., & Wang, X. (2023) · Pest Management Science · doi:10.1002/ps.7847 · Cited by 44
Multiplexed CRISPR-Based Nucleic Acid Detection Using a Single Cas Protein
Fu, J., Zhang, L., Long, Y., Liu, Z., Meng, G., Zhao, H., Su, X., & Shi, S. (2023) · Analytical Chemistry · doi:10.1021/acs.analchem.3c01861 · Cited by 44
PAM‐Engineered Toehold Switches as Input‐Responsive Activators of CRISPR‐Cas12a for Sensing Applications
Bagheri, N., Chamorro-García, A., Idili, A., & Porchetta, A. (2024) · Angewandte Chemie International Edition · doi:10.1002/anie.202319677 · Cited by 44
A handheld isothermal fluorescence detector for duplex visualization of aquatic pathogens via enhanced one-pot LAMP-PfAgo assay
Pang, F., Zhang, T., Dai, F., Wang, K., Jiao, T., Zhang, Z., Zhang, L., Liu, M., Hu, P., & Song, J. (2024) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2024.116187 · Cited by 44
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Sun, K. W., Pu, L., Chen, C., Chen, M., Li, K., Li, X., Li, H., & Geng, J. (2024) · Nucleic Acids Research · doi:10.1093/nar/gkae176 · Cited by 52
DNA-targeting short Argonautes complex with effector proteins for collateral nuclease activity and bacterial population immunity
Prostova, M. A., Kanevskaya, A., Panteleev, V., Lisitskaya, L., Tugaeva, K. V., Sluchanko, N. N., Esyunina, D., & Kulbachinskiy, A. V. (2024) · Nature Microbiology · doi:10.1038/s41564-024-01654-5 · Cited by 43
PAM-flexible Engineered FnCas9 variants for robust and ultra-precise genome editing and diagnostics
Acharya, S., Ansari, A. H., Das, P. K., Hirano, S., Aich, M., Rauthan, R., Mahato, S., Maddileti, S., Sarkar, S., Kumar, M., et al. (2024) · Nature Communications · doi:10.1038/s41467-024-49233-w · Cited by 47
Terminal Chemical Modifications of crRNAs Enable Improvement in the Performance of CRISPR-Cas for Point-of-Care Nucleic Acid Detection
Wan, Y., Li, S., Xu, W., Wang, K., Guo, W., Yang, C., Li, X., Zhou, J., & Wang, J. (2024) · Analytical Chemistry · doi:10.1021/acs.analchem.4c03698 · Cited by 44
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Alsharksi, A. N., Sirekbasan, S., Tan, T. G., & Mustapha, A. (2024) · Diagnostics · doi:10.3390/diagnostics14242876 · Cited by 55
One‐Pot Isothermal Nucleic Acid Amplification Assisted CRISPR/Cas Detection Technology: Challenges, Strategies, and Perspectives
Liu, H., Yin, H. Y., Xiu, L., Wu, W., Hu, Q., Xia, Y., Garcia, B., Shifa, S., Chen, H., Li, M., et al. (2025) · Advanced Science · doi:10.1002/advs.202506716 · Cited by 44
CRISPR‐driven diagnostics: Molecular mechanisms, clinical efficacy and translational challenges
Wang, Z., Wang, Q., Zhang, J., Li, B., Li, Y., Chen, Z., Guo, D., & Feng, S. (2025) · Clinical and Translational Medicine · doi:10.1002/ctm2.70482 · Cited by 51
Applying biosensor development concepts to improve preamplification-free CRISPR/Cas12a-Dx
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Type III-A CRISPR immunity promotes mutagenesis of staphylococci
Mo, C. Y., Mathai, J., Rostøl, J. T., Varble, A., Banh, D. V., & Marraffini, L. A. (2021) · Nature · doi:10.1038/s41586-021-03440-3 · Cited by 44
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Zhang, Y., Huang, Z., Zhu, J., Li, C., Fang, Z., Chen, K., & Zhang, Y. (2022) · Bioengineering & Translational Medicine · doi:10.1002/btm2.10356 · Cited by 45
Emerging applications of gene editing technologies for the development of climate-resilient crops
Chavhan, R. L., Jaybhaye, S. G., Hinge, V. R., Deshmukh, A. S., Shaikh, U. S., Jadhav, P. K., Kadam, U. S., & Hong, J. C. (2025) · Frontiers in Genome Editing · doi:10.3389/fgeed.2025.1524767 · Cited by 55
Navigating the Pandemic Response Life Cycle: Molecular Diagnostics and Immunoassays in the Context of COVID-19 Management
Gharizadeh, B., Yue, J., Yu, M., Liu, Y., Zhou, M., Lu, D., & Zhang, J. (2020) · IEEE Reviews in Biomedical Engineering · doi:10.1109/rbme.2020.2991444 · Cited by 42
Detection of viral RNAs at ambient temperature via reporter proteins produced through the target-splinted ligation of DNA probes
Phillips, E. A., Silverman, A. D., Joneja, A., Liu, M., Brown, C. W., Carlson, P. D., Coticchia, C. M., Shytle, K., Larsen, A., Goyal, N., et al. (2023) · Nature Biomedical Engineering · doi:10.1038/s41551-023-01028-y · Cited by 44
Tandem CRISPR nucleases-based lateral flow assay for amplification-free miRNA detection via the designed “locked RNA/DNA” as fuels
Tian, B., Wang, Y., Tang, W., Chen, J., Zhang, J., Xue, S., Cheng, G., Cheng, G., Gu, B., & Chen, M. (2023) · Talanta · doi:10.1016/j.talanta.2023.124995 · Cited by 42
Self-citation
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Nguyễn, L. T., Macaluso, N. C., Rakestraw, N. R., Carman, D. R., Pizzano, B. L. M., Hautamaki, R. C., Rananaware, S. R., Roberts, I. E., & Jain, P. (2024) · Cell Reports · doi:10.1016/j.celrep.2024.113777 · Cited by 43
High-yield porphyrin production through metabolic engineering and biocatalysis
Chen, H., Wang, Y., Wang, W., Cao, T., Zhang, L., Wang, Z., Chi, X., Shi, T., Wang, H., He, X., et al. (2024) · Nature Biotechnology · doi:10.1038/s41587-024-02267-3 · Cited by 46
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Liu, Y., Liu, X., Wei, D., Dang, L., Xu, X., Huang, S., Li, L., Wu, S., Wu, J., Liu, X., et al. (2024) · Nature Communications · doi:10.1038/s41467-024-49414-7 · Cited by 44
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Razavi, Z.-S., Soltani, M., Pazoki–Toroudi, H., & Chen, P. (2024) · Sensors and Actuators A Physical · doi:10.1016/j.sna.2024.115625 · Cited by 44
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Dunkley, O. R. S., Bell, A. G., Modi, N. H., Huang, Y., Tseng, S., Reiss, R., Daivaa, N., Davis, J. L., Vargas, D. A., Banada, P. P., et al. (2025) · Science Advances · doi:10.1126/sciadv.adx2067 · Cited by 43
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Li, B., Yan, J., Zhang, Y., Li, W., Zeng, C., Zhao, W., Hou, X., Zhang, C., & Dong, Y. (2020) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2019.12.038 · Cited by 43
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Wang, S., Fan, Y., Feng, Z., Song, M., Li, Q., Jiang, B., Qin, F., Liu, H., Lan, L., & MeiCheng, Y. (2021) · Food Control · doi:10.1016/j.foodcont.2021.108194 · Cited by 43
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You, Y., Zhang, P., Wu, G., Tan, Y., Zhao, Y. X., Cao, S., Song, Y., Yang, R., & Du, Z. (2021) · Frontiers in Microbiology · doi:10.3389/fmicb.2021.700016 · Cited by 43
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Liu, P., Wang, X., Liang, J. B., Dong, Q., Zhang, J., Liu, D., Wang, S., Bi, J., Liu, W., Wang, Z., et al. (2022) · Frontiers in Microbiology · doi:10.3389/fmicb.2021.796916 · Cited by 43
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Ye, H., Nowak, C. M., Liu, Y., Li, Y., Zhang, T., Bleris, L. G., & Qin, Z. (2022) · Small · doi:10.1002/smll.202107832 · Cited by 42
An automated nucleic acid detection platform using digital microfluidics with an optimized Cas12a system
Sun, Z. C., Lin, K., Zhao, Z., Wang, Y., Hong, X.-X., Guo, J., Ruan, Q., Lu, L., Li, X., Zhang, R., et al. (2022) · Science China Chemistry · doi:10.1007/s11426-021-1169-1 · Cited by 43
Combining CRISPR/Cas12a with isothermal exponential amplification as an ultrasensitive sensing platform for microRNA detection
Yang, Y., Yang, J., Gong, F., Zuo, P., Tan, Z., Li, J., Xie, C., Ji, X., Li, W., & He, Z. (2022) · Sensors and Actuators B Chemical · doi:10.1016/j.snb.2022.132158 · Cited by 43
Hybrid CRISPR/Cas protein for one-pot detection of DNA and RNA
Guk, K., Yi, S., Kim, H., Bae, Y., Yong, D., Kim, S., Lee, K., Lim, E., Kang, T., & Jung, J. (2022) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2022.114819 · Cited by 42
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Bao, Q., Sun, J., Fu, X., Sheng, L., Ye, Y., Ji, J., Zhang, Y., Wang, J.-S., Ping, J. F., & Sun, X. (2023) · Small · doi:10.1002/smll.202207343 · Cited by 42
Split activator of CRISPR/Cas12a for direct and sensitive detection of microRNA
He, W., Li, X., Li, X., Guo, M., Zhang, M., Hu, R., Li, M., Ding, S., & Yan, Y. (2024) · Analytica Chimica Acta · doi:10.1016/j.aca.2024.342477 · Cited by 42
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Synergetic performance of isothermal amplification techniques and lateral flow approach for nucleic acid diagnostics
Mukama, O., Nie, C., Habimana, J. D. D., Meng, X., Ting, Y., Fanuel, S., AL-Farga, A. M., Mugisha, S., Rwibasira, P., Zhang, Y., et al. (2020) · Analytical Biochemistry · doi:10.1016/j.ab.2020.113762 · Cited by 43
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Huang, W., Yu, L., Wen, D., Wei, D., Sun, Y., Zhao, H., Ye, Y., Chen, W., Zhu, Y., Wang, L., et al. (2020) · EBioMedicine · doi:10.1016/j.ebiom.2020.103036 · Cited by 42
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He, R., Wang, L., Wang, F., Yang, J., Yu, X., Wang, Y., Liu, Z., Li, C., & Ma, L. (2021) · The Analyst · doi:10.1039/d1an01521d · Cited by 42
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Authors: Chen, J. S., Ma, E., Harrington, L. B., Da Costa, M., Tian, X., Palefsky, J. M., & Doudna, J. A.
Affiliations: University of California, Berkeley (US)
Department of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA.
University of California, San Francisco (US)
Howard Hughes Medical Institute (US)
Corresponding author: Chen, Janice S.
Ma, Enbo
Harrington, Lucas B.
Doudna, Jennifer A.
ORCID: Chen, Janice S.
Harrington, Lucas B.
Da Costa, Maria
Tian, Xinran
Palefsky, Joel M.
Doudna, Jennifer A.
Venue: Science
Publisher: American Association for the Advancement of Science (AAAS)
Published: 2018-04-27
Volume: 360
Issue: 6387
Pages: 436-439
ISSN: 0036-8075 (ISSN-L)
1095-9203
DOI: 10.1126/science.aar6245
PMC: PMC6628903
OpenAlex: W2787024379
Cited by: 4,507
References: 48
Citations by year: 2026: 759 · 2025: 816 · 2024: 736 · 2023: 643 · 2022: 624 · 2021: 452 · 2020: 271 · 2019: 135 · 2018: 67
FWCI: 82.27
Type: journal article
Language: en
Open access: green
doi.org
Locations: Science (publishedVersion)
bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
PubMed (publishedVersion)
Europe PMC (PubMed Central) (acceptedVersion)
PubMed Central (acceptedVersion)
eScholarship (California Digital Library) (submittedVersion)
Funders: National Science Foundation (MCB-1244557)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
Keywords: CRISPR/Cas12a, single-stranded DNA cleavage, isothermal amplification, DETECTR, DNA detection, HPV testing, molecular diagnosis, genome editing
MeSH terms: Clostridiales, Eubacteriales, Bacterial Proteins, DNA, Single-Stranded, Endodeoxyribonucleases, Endonucleases, Kinetics, Substrate Specificity, DNA Cleavage, CRISPR-Cas Systems, CRISPR-Associated Proteins

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CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

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