Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants

Cited by (1,002)

Items 601–700 of 1,002

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4. Nucleases in gene-editing technologies: past and prologue
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5. Cooperativity between Cas9 and hyperactive AID establishes broad and diversifying mutational footprints in base editors
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7. Graded-CRISPRi, a Tool for Tuning the Strengths of CRISPRi-Mediated Knockdowns in Vibrio natriegens Using gRNA Libraries
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8. Genome editing to define the function of risk loci and variants in rheumatic disease
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9. Evaluation and prediction of guide RNA activities in genome-editing tools
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10. Methods for detecting off-target effects of CRISPR/Cas9
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11. CRISPR–Cas-Mediated Reprogramming Strategies to Overcome Antimicrobial Resistance
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12. ABE8e Corrects Pax6-Aniridic Variant in Humanized Mouse ESCs and via LNPs in Ex Vivo Cortical Neurons
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13. RNA modifications in pulmonary diseases
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14. Identification of functional sgRNA mutants lacking canonical secondary structure using high-throughput FACS screening
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15. SPLICER: a highly efficient base editing toolbox that enables in vivo therapeutic exon skipping
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16. Treating genetic blood disorders in the era of CRISPR-mediated genome editing
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17. Gene Editing in Ganoderma lucidum: Development, Challenges, and Future Prospects
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18. Shining light on CRISPR/Cas9 therapeutics for inherited retinal diseases
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19. Charting the development and engineering of CRISPR base editors: lessons and inspirations
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20. Translating genomic insights into cardiovascular medicine: Opportunities and challenges of CRISPR-Cas9
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21. SpRY Cas9 Can Utilize a Variety of Protospacer Adjacent Motif Site Sequences To Edit the Candida albicans Genome
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22. Making the cut with PAMless CRISPR-Cas enzymes
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23. Engineering an adenine base editor in human embryonic stem cells with minimal DNA and RNA off-target activities
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24. Screening an Effective Dual-Adeno-Associated Virus Split-Cytosine Base Editor System for C-to-T Conversion In Vivo
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25. Novel CRISPR/SpRY system for rapid detection of CRISPR/Cas-mediated gene editing in rice
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26. High-throughput tagging of endogenous loci for rapid characterization of protein function
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27. CRISPR/Cas-based Human T cell Engineering: Basic Research and Clinical Application
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28. Craspase: A novel CRISPR/Cas dual gene editor
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29. Turbo‐charging crop improvement: harnessing multiplex editing for polygenic trait engineering and beyond
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30. AND logic-gated CRISPR/Cas9 and hybridization chain reaction system for precise ctDNA detection
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31. Functional knockout of long non-coding RNAs with genome editing
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32. Efficient CRISPR‐mediated C‐to‐T base editing in Komagataella phaffii
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33. Comprehensive evaluation and prediction of editing outcomes for near-PAMless adenine and cytosine base editors
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34. Easy‐Curing and pH ‐Regulated CRISPR ‐Cas9 Plasmids for Gene Editing and Plasmid Curing in Lactococcus cremoris
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35. Integrative Approaches to Myopathies and Muscular Dystrophies: Molecular Mechanisms, Diagnostics, and Future Therapies
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36. Plant base editing: a decade of progress and future applications
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37. Analysis of conventional and alternative CRISPR/Cas9 genome editing to enhance a single-base pair knock-in mutation
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38. Expanding the target range of base editing in plants without loss of efficiency by blocking RNA‐silencing
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39. Highly efficient A-to-G base editing by ABE8.17 in rabbits
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40. Gene editing of Duchenne muscular dystrophy using biomineralization-based spCas9 variant nanoparticles
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41. Adenine base editor‐based correction of the cardiac pathogenic Lmna c. 1621C > T mutation in murine hearts
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42. Accurate top protein variant discovery via low-N pick-and-validate machine learning
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43. Expanding the flexibility of base editing for high-throughput genetic screens in bacteria
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44. High-Throughput Screening of PAM-Flexible Cas9 Variants for Expanded Genome Editing in the Silkworm (Bombyx mori)
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45. PAMless SpRY exhibits a preference for the seed region for efficient targeting
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46. Direct delivery of Cas-embedded cytosine base editors as ribonucleoprotein complexes for efficient and accurate editing of clinically relevant targets
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47. Using omics data and genome editing methods to decipher GWAS loci associated with coronary artery disease
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48. Matrix regulation: a plug-and-tune method for combinatorial regulation in Saccharomyces cerevisiae
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49. In vivo base editing reduces liver cysts in autosomal dominant polycystic kidney disease
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50. Multiplex base editing of BCL11A regulatory elements to treat sickle cell disease
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51. Activity-based selection for enhanced base editor mutational scanning
    E L Kaplan, Ryan J. Steger, Spencer T. Shah, Laura M. Drepanos, Audrey L Griffith, Ganna Reint, John G. Doench · 2025 · Nature Genetics
52. Programmable Multiplex Genome Editing: Innovations in CRISPR Effectors, crRNA Engineering, and Delivery Strategies
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53. Uncovering Cas9 PAM diversity through metagenomic mining and machine learning
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54. Genome Engineering in Plant Using an Efficient CRISPR-xCas9 Toolset With an Expanded PAM Compatibility
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55. Towards a CRISPeR understanding of homologous recombination with high-throughput functional genomics
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56. Chimeric RNA: DNA TracrRNA Improves Homology-Directed Repair In Vitro and In Vivo
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57. Recent Advances and Therapeutic Strategies Using CRISPR Genome Editing Technique for the Treatment of Cancer
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58. Hematopoietic stem and progenitors cells gene editing: Beyond blood disorders
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59. Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e
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60. Vector enabled CRISPR gene editing – A revolutionary strategy for targeting the diversity of brain pathologies
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61. Developing a PAM-Flexible CRISPR-Mediated Dual-Deaminase Base Editor to Regulate Extracellular Electron Transport in Shewanella oneidensis
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62. Bacterial synthetic biology: tools for novel drug discovery
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63. CRISPR-based precision medicine for hematologic disorders: Advancements, challenges, and prospects
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64. Engineered domain-inlaid Nme2Cas9 adenine base editors with increased on-target DNA editing and targeting scope
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65. Deconstructing cancer with precision genome editing
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66. Broadening the targetable space: engineering and discovery of PAM-flexible Cas proteins
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67. A comprehensive all-in-one CRISPR toolbox for large-scale screens in plants
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69. AddTag, a two-step approach with supporting software package that facilitates CRISPR/Cas-mediated precision genome editing
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    Wei Yang, Wei Qi, Yucai Li, Jiyao Wang, Yanmin Luo, Dehui Ding, Sudong Mo, Bo Chen, Yu Lu, Huarong Li et al. · 2020 · Science China Life Sciences
77. A utilização da técnica de CRISPR-CAS9 na Terapia Gênica
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81. Amino acid-derived ionizable lipids enable inhaled base editing for therapeutic gene correction in the lung
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86. Epigenetic editing for autosomal dominant neurological disorders
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100. Use of paired Cas9-NG nickase and truncated sgRNAs for single-nucleotide microbial genome editing
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