Cited by 367Chemical Synthesis and AnalysisInnovative Microfluidic and Catalytic Techniques InnovationRNA and protein synthesis mechanisms

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Synthesis of proteins by automated flow chemistry

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Hartrampf, N., Saebi, A., Poskus, M., Gates, Z. P., Callahan, A. J., Cowfer, A. E., Hanna, S., Antilla, S., Schissel, C. K., Quartararo, A. J., Ye, X., Mijalis, A. J., Simon, M. D., Loas, A., Liu, S., Jessen, C., Nielsen, T. E., Pentelute, B. L.·2020·Science

DOI 10.1126/science.abb2491

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Abstract

Ribosomes can produce proteins in minutes and are largely constrained to proteinogenic amino acids. Here, we report highly efficient chemistry matched with an automated fast-flow instrument for the direct manufacturing of peptide chains up to 164 amino acids long over 327 consecutive reactions. The machine is rapid: Peptide chain elongation is complete in hours. We demonstrate the utility of this approach by the chemical synthesis of nine different protein chains that represent enzymes, structural units, and regulatory factors. After purification and folding, the synthetic materials display biophysical and enzymatic properties comparable to the biologically expressed proteins. High-fidelity automated flow chemistry is an alternative for producing single-domain proteins without the ribosome.

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M. D. Simon “Fast flow biopolymer synthesis ” thesis Massachusetts Institute of Technology Cambridge MA (2017).
10.1002/cbic.201300796 · DOI · Paper page

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Authors: Hartrampf, N., Saebi, A., Poskus, M., Gates, Z. P., Callahan, A. J., Cowfer, A. E., Hanna, S., Antilla, S., Schissel, C. K., Quartararo, A. J., Ye, X., Mijalis, A. J., Simon, M. D., Loas, A., Liu, S., Jessen, C., Nielsen, T. E., & Pentelute, B. L.
Affiliations: Massachusetts Institute of Technology (US)
Novo Nordisk (Denmark) (DK)
Corresponding author: Saebi, A.
Poskus, M.
Pentelute, B. L.
ORCID: Hartrampf, N.
Saebi, A.
Poskus, M.
Gates, Z. P.
Callahan, A. J.
Cowfer, A. E.
Antilla, S.
Schissel, C. K.
Quartararo, A. J.
Ye, X.
Mijalis, A. J.
Loas, A.
Jessen, C.
Nielsen, T. E.
Pentelute, B. L.
Venue: Science
Publisher: American Association for the Advancement of Science (AAAS)
Published: 2020-05-29
Volume: 368
Issue: 6494
Pages: 980-987
ISSN: 0036-8075 (ISSN-L)
1095-9203
DOI: 10.1126/science.abb2491
OpenAlex: W3029756541
Cited by: 367
References: 72
Citations by year: 2026: 35 · 2025: 61 · 2024: 76 · 2023: 63 · 2022: 44 · 2021: 61 · 2020: 25
FWCI: 15.65
Type: journal article
Language: en
Open access: green
doi.org
Licence: cc-by-nc-nd
Locations: Science (publishedVersion)
ChemRxiv (acceptedVersion, cc-by-nc-nd)
PubMed (publishedVersion)
DSpace@MIT (Massachusetts Institute of Technology) (submittedVersion, cc-by-nc-sa)
Royal College of Surgeons in Ireland (submittedVersion, cc-by-nc-nd)
Funders: National Science Foundation (1122374)
Novo Nordisk
Topics: Physical Sciences › Chemistry › Organic Chemistry › Chemical Synthesis and Analysis
Physical Sciences › Chemical Engineering › Fluid Flow and Transfer Processes › Innovative Microfluidic and Catalytic Techniques Innovation
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
Keywords: protein synthesis, translation elongation, single-domain proteins, protein folding, enzyme activity, protein purification
MeSH terms: Protein Domains, Peptides, Proteins, Protein Folding, Solid-Phase Synthesis Techniques

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Synthesis of proteins by automated flow chemistry

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