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Cited by 67Neurogenesis and neuroplasticity mechanismsDevelopmental Biology and Gene RegulationSingle-cell and spatial transcriptomics

Fate mapping of neural stem cell niches reveals distinct origins of human cortical astrocytes

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Allen, Denise E., Donohue, Kevin C., Cadwell, Cathryn R., Shin, David, Keefe, Matthew G., Sohal, Vikaas S., Nowakowski, Tomasz J.·2022·Science
DOI 10.1126/science.abm5224PMC PMC9233096
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Abstract

Progenitors of the developing human neocortex reside in the ventricular and outer subventricular zones (VZ and OSVZ, respectively). However, whether cells derived from these niches have similar developmental fates is unknown. By performing fate mapping in primary human tissue, we demonstrate that astrocytes derived from these niches populate anatomically distinct layers. Cortical plate astrocytes emerge from VZ progenitors and proliferate locally, while putative white matter astrocytes are morphologically heterogeneous and emerge from both VZ and OSVZ progenitors. Furthermore, via single-cell sequencing of morphologically defined astrocyte subtypes using Patch-seq, we identify molecular distinctions between VZ-derived cortical plate astrocytes and OSVZ-derived white matter astrocytes that persist into adulthood. Together, our study highlights a complex role for cell lineage in the diversification of human neocortical astrocytes.

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Cited by

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Authors
Allen, D. E., Donohue, K. C., Cadwell, C. R., Shin, D., Keefe, M. G., Sohal, V. S., & Nowakowski, T. J.
Affiliations
University of California, San Francisco (US)
Corresponding author
Nowakowski, Tomasz J.
ORCID
Venue
Science
Publisher
American Association for the Advancement of Science (AAAS)
Published
2022-06-24
Volume
376
Issue
6600
Pages
1441-1446
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
DOI
10.1126/science.abm5224
PMC
PMC9233096
OpenAlex
W4280643668
Cited by
67
References
7
Citations by year
2026: 10 · 2025: 14 · 2024: 18 · 2023: 18 · 2022: 7
FWCI
5.23
Type
journal article
Language
en
Open access
Locations
  • Science (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
  • eScholarship (California Digital Library) (submittedVersion)
Funders
  • Simons Foundation Autism Research Initiative (SFARI 491371)
  • National Institutes of Health (NS070680)
  • University of California, San Francisco
  • National Institute of Mental Health (RF1MH121268, U01MH115747)
  • National Cancer Institute (P50CA097257)
  • National Institute of Neurological Disorders and Stroke (R25NS070680, P01NS083513, K08NS126573, R01NS123263)
  • Eunice Kennedy Shriver National Institute of Child Health and Human Development (T32HD007470)
Topics
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Single-cell and spatial transcriptomics
Keywords
cortical astrocytes, ventricular zone, outer subventricular zone, human neocortex development, fate mapping, white matter astrocytes, astrocyte subtypes, Patch-seq, cell lineage, astrocyte heterogeneity
MeSH terms
Astrocytes, Humans, Cell Lineage, Neocortex, Stem Cell Niche, Neurogenesis, Neural Stem Cells, Primary Cell Culture

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