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Origin of the U87MG glioma cell line: Good news and bad news
Abstract
The DNA profile of the publicly available and widely used glioma cell line U87MG is quite different from that of the original cell line.
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“studies using the classic U87MG and U251MG glioma cell lines have shown that when grown in serum-containing medium these glioma cells exhibit transcrip-tomic deviation from the original tumour from which they were derived63.”
From How to design preclinical studies in nanomedicine and cell therapy to maximize the prospects of clinical translation (2018) · cites this paper as [63]
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Check your cultures! A list of cross‐contaminated or misidentified cell lines
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“Theproblemof themisidentification of cell lines has beenhighlighted in several reports (11, 12), and a number of scientific journals now want the identity of the samples and cell lines used in their publication to be confirmed.”
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- Retracted
OECD (2018) · OECD series on testing and assessment · doi:10.1787/9789264304796-14-en
List of viability testing methods (non-inclusive) of cell cultures
OECD (2018) · OECD series on testing and assessment · doi:10.1787/9789264304796-24-en
Глиобластома и стволовые клетки костного мозга
Брюховецкий, И. С., Брюховецкий, А. С., Хотимченко, Ю. С., Пак, О. И., Zaitsev, S., Шевченко, В. Е., & Lyakhova, I. (2020) · Publishing House of Far Eastern Federal University eBooks · doi:10.24866/7444-4837-0
In Vitro Antiproliferative and Free Radical Scavenging Activities in Peels of Citrus Fruits
Pallavi, M., Ramesh, C. K., Krishna, V., & Parveen, S. (2018) · Research Journal of Life Sciences Bioinformatics Pharmaceutical and Chemical Sciences · doi:10.26479/2018.0404.26
Metadata
- Authors
- Allen, M., Bjerke, M., Edlund, H., Nelander, S., & Westermark, B.
- Affiliations
- Uppsala University (SE)
- Corresponding author
- Westermark, Bengt
- Venue
- Science Translational Medicine
- Published
- 2016-08-31
- Volume
- 8
- Issue
- 354
- Pages
- 354re3-354re3
- ISSN
- 1946-6234 (ISSN-L)
- 1946-6242
- OpenAlex
- W2509121458
- Cited by
- 466
- References
- 15
- Citations by year
- 2026: 28 · 2025: 33 · 2024: 43 · 2023: 36 · 2022: 49 · 2021: 41 · 2020: 59 · 2019: 51 · 2018: 84 · 2017: 37 · 2016: 5
- FWCI
- 42.81
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science Translational Medicine (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Swedish Cancer Society
- Swedish Crime Victim Compensation and Support Authority
- Topics
- Health Sciences › Medicine › Oncology › Glioma Diagnosis and Treatment
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer Genomics and Diagnostics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene expression and cancer classification
- Keywords
- glioma cell lines, glioblastoma, tumor origin, genetic profiling, gene expression profiling, cell line misidentification, translational oncology
- MeSH terms
- Brain Neoplasms, DNA, Mitochondrial, DNA, Neoplasm, Glioblastoma, Glioma, Humans, DNA Fingerprinting, Microsatellite Repeats, Cell Culture Techniques, Gene Expression Profiling, Cell Line, Tumor, Translational Research, Biomedical
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