Biofilm production and antibiofilm activity of echinocandins and liposomal amphotericin B in echinocandin-resistant yeast species
Open accessAbstract
The echinocandins and liposomal amphotericin B are active against biofilm produced by echinocandin-susceptible Candida strains. However, few data have been reported on the production of biofilm by echinocandin-resistant isolates and their antifungal susceptibility. We studied the production of biofilm by fks mutant Candida strains and intrinsically echinocandin-resistant non- Candida isolates and the susceptibility of both entities to liposomal amphotericin B and echinocandins. We analyzed the production of biofilm by isolates from patients with fungemia ( fks mutant Candida , n = 5; intrinsically echinocandin-resistant non- Candida , n = 12; and Candida wild type, n = 10). Biofilm formation was measured to classify strains according to biomass (crystal violet assay) and metabolic activity (XTT reduction assay). Preformed biofilms were tested against liposomal amphotericin B, caspofungin, micafungin, and anidulafungin. The sessile MIC was defined as the antifungal concentration yielding a 50% or 80% reduction in the metabolic activity of the biofilm compared to that of the growth control (SMIC 50 and SMIC 80 , respectively). fks mutant Candida isolates formed biofilms in a fashion similar to that of Candida wild-type strains. The echinocandins had the highest activity against biofilms formed by wild-type Candida isolates, followed by fks mutant Candida isolates and non- Candida isolates. Liposomal amphotericin B had the highest activity against fks mutant Candida biofilms. The formation of biofilm by echinocandin-resistant strains was similar to that of wild-type strains, although resistance to echinocandins remained high.
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Early work in the late 1990s and early 2000s demonstrated potent antifungal activity of physiological concentrations of echinocandins against C. albicans biofilms, with subsequent studies extended to other Candida spp.
“Early work in these academic laboratories in the late 1990s and early 2000s demonstrated the potent antifungal activity of physiological concentrations of echinocandins against C. albicans biofilms, with subsequent studies extended to other Candida spp [34,37,38].”
From Antifungal therapy of Candida biofilms: Past, present and future (2023) · cites this paper as [38]
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Self-citation
- Authors
- Marcos-Zambrano, L. J., Gómez-Perosanz, M., Escribano, P., Zaragoza, O., Bouza, E., & Guinea, J.
- Affiliations
- Hospital General Universitario Gregorio Marañón (ES)
- Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Marañón, Madrid, Spain
- Mycology Reference Laboratory, National Centre for Microbiology, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain
- Universidad Complutense de Madrid (ES)
- Venue
- Antimicrobial Agents and Chemotherapy
- Publisher
- American Society for Microbiology
- Published
- 2016-06
- Volume
- 60
- Issue
- 6
- Pages
- 3579-3586
- ISSN
- 0066-4804 (ISSN-L)
- 1098-6596
- PMC
- PMC4879372
- OpenAlex
- W2400312947
- Cited by
- 28
- References
- 28
- Citations by year
- 2026: 4 · 2025: 3 · 2024: 2 · 2023: 3 · 2022: 2 · 2021: 1 · 2020: 3 · 2019: 5 · 2018: 1 · 2017: 4
- FWCI
- 3.08
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Antimicrobial Agents and Chemotherapy (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (submittedVersion, other-oa)
- Funders
- Fondo de Investigación Sanitaria (PI11/00167, PI14/00740)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Health Sciences › Medicine › Epidemiology › Fungal Infections and Studies
- Keywords
- biofilm formation, echinocandin resistance, liposomal amphotericin B, Candida, caspofungin, micafungin, anidulafungin, fungemia, antifungal susceptibility
- MeSH terms
- Caspofungin, Micafungin, Anidulafungin, Amphotericin B, Antifungal Agents, Candida, Candidiasis, Glucosyltransferases, Humans, Microbial Sensitivity Tests, Biofilms, Drug Resistance, Fungal, Echinocandins, Lipopeptides
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