EUCAST technical note on the EUCAST definitive document EDef 7.2: method for the determination of broth dilution minimum inhibitory concentrations of antifungal agents for yeasts EDef 7.2 (EUCAST-AFST)
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The European Committee on Antimicrobial Susceptibility Testing-Subcommittee on Antifungal Susceptibility Testing (EUCAST-AFST) has revised the EDef 7.1 document on the method for the determination of broth dilution minimum inhibitory concentrations of antifungal agents for fermentative yeasts. Changes are: dimethylsulphoxide is now the recommended solvent for caspofungin, micafungin and fluconazole; the shelf-life of plates containing the echinocandins prepared from stock solutions in dimethylsulphoxide is extended to 6 months at -80°C; testing of amphotericin and Cryptococcus has been incorporated; and minimum inhibitory concentration ranges for quality control strains and anidulafungin are included.
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Susceptibility tests were performed in at least three replicates following the EUCAST E.DEF 7.3.1 protocol.
“Susceptibility tests were performed in at least three replicates following the EUCAST E.DEF 7.3.1. protocol.64”
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Updated antifungal susceptibility testing reference methods identify echinocandin-resistant isolates reliably.
“The updated antifungal susceptibility testing reference methods identify echinocandin resistant isolates reliably; while the performance of commercial tests is somewhat more variable.”
From Echinocandin resistance: an emerging clinical problem? (2014) · cites this paper as [37]
Lackner et al found reduced kidney burden comparing sequential C. albicans clinical isolates with and without two heterogenous mutations simultaneously at position R647 and P649.
“Similarly, Lackner et al found reduced kidney burden comparing sequential C. albicans clinical isolates with and without two heterogenous mutations simultaneously at position R647 and P649 (34).”
From Echinocandin resistance: an emerging clinical problem? (2014) · cites this paper as [34]
Antifungal susceptibility testing has been optimised over recent years.
“Antifungal susceptibility testing has been optimised over the recent years and should be available real time for centres managing high-risk patients.”
From Echinocandin resistance: an emerging clinical problem? (2014) · cites this paper as [37]
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- Authors
- Arendrup, M. C., Cuenca-Estrella, M., Lass-Flörl, C., & Hope, W.
- Affiliations
- Statens Serum Institut (DK)
- Manchester Academic Health Science Centre (GB)
- Corresponding author
- Arendrup, Maiken C.
- Venue
- Clinical Microbiology and Infection
- Publisher
- Elsevier BV
- Published
- 2012-07
- Volume
- 18
- Issue
- 7
- Pages
- E246-E247
- ISSN
- 1198-743X (ISSN-L)
- OpenAlex
- W1900369848
- Cited by
- 451
- References
- 8
- Citations by year
- 2026: 18 · 2025: 18 · 2024: 31 · 2023: 34 · 2022: 35 · 2021: 38 · 2020: 31 · 2019: 25 · 2018: 38 · 2017: 40 · 2016: 39 · 2015: 33 · 2014: 41 · 2013: 23 · 2012: 7
- FWCI
- 23.07
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Clinical Microbiology and Infection (publishedVersion)
- PubMed (publishedVersion)
- Radboud Repository (Radboud University) (submittedVersion)
- Funders
- National Institutes of Health
- National Institute for Health and Care Research
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Bacterial Identification and Susceptibility Testing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Yeasts and Rust Fungi Studies
- Keywords
- antifungal susceptibility testing, broth dilution, minimum inhibitory concentration, fermentative yeasts, caspofungin, micafungin, fluconazole, echinocandins, dimethyl sulfoxide, amphotericin, Cryptococcus, anidulafungin, quality control strains, EUCAST
- MeSH terms
- Antifungal Agents, Europe, Freezing, Humans, Microbial Sensitivity Tests, Solvents, Time Factors, Yeasts
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