Role of cell defense against oxidative damage in the resistance of Candida albicans to the killing effect of amphotericin B
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A laboratory-derived mutant of Candida albicans B311 (L) and a clinical isolate (C) of C. albicans, both lacking membrane ergosterol, were less susceptible to amphotericin B (AmB)-induced cell membrane permeability to K+ and lethality than was the wild-type laboratory strain (B311) which contained ergosterol. The resistance of L and C to AmB-induced killing was much greater than the level of resistance to AmB-induced cell membrane permeability. L and C were also less susceptible to killing by H2O2 than was B311, and when treated with menadione, they each produced less H2O2 than did B311. In addition, their levels of catalase activity were 3.8-fold (L) and 2-fold (C) higher than that of B311. The ergosterol deficiency in L and C probably impaired AmB binding to the cells, thereby lowering AmB effectiveness as measured by both cell membrane permeability and killing. Resistance of strains L and C to oxidation-dependent damage likely contributed to a diminished response to AmB-induced lethality.
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Earlier studies pointed to a correlation between withstanding oxidative stress and AmB resistance in other fungal species.
“earlier studies pointed to a correlation between withstanding oxidative stress and AmB resistance in other fungal species [58].”
From Lansoprazole interferes with fungal respiration and acts synergistically with amphotericin B against multidrug-resistant <i>Candida auris</i> (2024) · cites this paper as [58]
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- Authors
- Sokol-Anderson, M., Sligh, J. E., Elberg, S., Brajtburg, J., Kobayashi, G. S., & Medoff, G.
- Affiliations
- Washington University in St. Louis (US)
- Venue
- Antimicrobial Agents and Chemotherapy
- Publisher
- American Society for Microbiology
- Published
- 1988-05
- Volume
- 32
- Issue
- 5
- Pages
- 702-705
- ISSN
- 0066-4804 (ISSN-L)
- 1098-6596
- PMC
- PMC172255
- OpenAlex
- W2069801130
- Cited by
- 113
- References
- 16
- Citations by year
- 2026: 1 · 2024: 3 · 2023: 5 · 2022: 3 · 2021: 3 · 2020: 3 · 2019: 4 · 2018: 2 · 2017: 7 · 2016: 4 · 2015: 3 · 2014: 2 · 2013: 4 · 2012: 4
- FWCI
- 1.62
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Antimicrobial Agents and Chemotherapy (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- National Institute of Allergy and Infectious Diseases (AI 07172, AI 07015, AI-16228)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Health Sciences › Medicine › Epidemiology › Fungal Infections and Studies
- Keywords
- Candida albicans, amphotericin B resistance, oxidative stress, cell membrane permeability, hydrogen peroxide, menadione, catalase activity, antifungal resistance, cell membrane damage
- MeSH terms
- Amphotericin B, Candida albicans, Catalase, Cell Membrane, Cell Membrane Permeability, Drug Resistance, Microbial, Ergosterol, Humans, Hydrogen Peroxide, Oxidation-Reduction
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