The DOI matches its Crossref record; 46 of the 46 references it cites were found in a registry or an index.
Engineering of Saccharomyces cerevisiae for Efficient Anaerobic Alcoholic Fermentation of l -Arabinose
Open accessAbstract
For cost-effective and efficient ethanol production from lignocellulosic fractions of plant biomass, the conversion of not only major constituents, such as glucose and xylose, but also less predominant sugars, such as l -arabinose, is required. Wild-type strains of Saccharomyces cerevisiae , the organism used in industrial ethanol production, cannot ferment xylose and arabinose. Although metabolic and evolutionary engineering has enabled the efficient alcoholic fermentation of xylose under anaerobic conditions, the conversion of l -arabinose into ethanol by engineered S. cerevisiae strains has previously been demonstrated only under oxygen-limited conditions. This study reports the first case of fast and efficient anaerobic alcoholic fermentation of l -arabinose by an engineered S. cerevisiae strain. This fermentation was achieved by combining the expression of the structural genes for the l -arabinose utilization pathway of Lactobacillus plantarum , the overexpression of the S. cerevisiae genes encoding the enzymes of the nonoxidative pentose phosphate pathway, and extensive evolutionary engineering. The resulting S. cerevisiae strain exhibited high rates of arabinose consumption (0.70 g h −1 g [dry weight] −1 ) and ethanol production (0.29 g h −1 g [dry weight] −1 ) and a high ethanol yield (0.43 g g −1 ) during anaerobic growth on l -arabinose as the sole carbon source. In addition, efficient ethanol production from sugar mixtures containing glucose and arabinose, which is crucial for application in industrial ethanol production, was achieved.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“Other studies used ethylmethanesulfonate (EMS) treated Bacillus boroniphilus to select for increased boron resistance [88] or EMS-treated S. cerevisiae in order to achieve increased nutrient utilization and stress resistance [95,113,114].”
From Adaptive laboratory evolution—Principles and applications for biotechnology (2013) · cites this paper as [88]
Citations
Cites
Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method
Livak, K. J., & Schmittgen, T. D. (2001) · Methods · doi:10.1006/meth.2001.1262 · Cited by 180,860
Molecular Cloning: A Laboratory Manual
Sambrook, J., Fritsch, E., & Maniatis, T. (1989) · UCLA - Biblioteca de Ciencias de la Salud · Cited by 133,589
Molecular Cloning. A Laboratory Manual
Wood, E. (1983) · Biochemical Education · doi:10.1016/0307-4412(83)90068-7 · Cited by 29,534
Mutation of the mouse klotho gene leads to a syndrome resembling ageing
Kuro-o, M., Matsumura, Y., Aizawa, H., Kawaguchi, H., Suga, T., Utsugi, T., Ohyama, Y., Kurabayashi, M., Kaname, T., Kume, E., et al. (1997) · Nature · doi:10.1038/36285 · Cited by 3,837
Transformation of yeast by lithium acetate/single-stranded carrier DNA/polyethylene glycol method
Gietz, R. D., & Woods, R. A. (2002) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(02)50957-5 · Cited by 2,855
Gietz, R. D., & Sugino, A. (1988) · Gene · doi:10.1016/0378-1119(88)90185-0 · Cited by 2,851
High efficiency transformation of Escherichia coli with plasmids
Inoue, H., Nojima, H., & Okayama, H. (1990) · Gene · doi:10.1016/0378-1119(90)90336-p · Cited by 2,315
A new efficient gene disruption cassette for repeated use in budding yeast
Güldener, U. (1996) · Nucleic Acids Research · doi:10.1093/nar/24.13.2519 · Cited by 1,657
Verduyn, C., Postma, E., Scheffers, W. A., & van Dijken, J. P. (1992) · Yeast · doi:10.1002/yea.320080703 · Cited by 1,510
Bacteria engineered for fuel ethanol production: current status
Dien, B. S., Cotta, M. A., & Jeffries, T. W. (2003) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-003-1444-y · Cited by 796
Lynd, L. R. (1996) · Annual Review of Energy and the Environment · doi:10.1146/annurev.energy.21.1.403 · Cited by 775
Experiments with the Chemostat on Spontaneous Mutations of Bacteria
Novick, A., & Szilard, L. (1950) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.36.12.708 · Cited by 725
Andreasen, A. A., & Stier, T. J. B. (1953) · Journal of Cellular and Comparative Physiology · doi:10.1002/jcp.1030410103 · Cited by 555
van Maris, A. J. A., Abbott, D. A., Bellissimi, E., van den Brink, J., Kuyper, M., Luttik, M. A. H., Wisselink, H. W., Scheffers, W. A., van Dijken, J. P., & Pronk, J. T. (2006) · Antonie van Leeuwenhoek · doi:10.1007/s10482-006-9085-7 · Cited by 546
Self-citationPhysiology of Saccharomyces Cerevisiae in Anaerobic Glucose-Limited Chemostat Culturesx
Verduyn, C., Postma, E., Scheffers, W. A., & van Dijken, J. P. (1990) · Journal of General Microbiology · doi:10.1099/00221287-136-3-395 · Cited by 471
Enzymatic Conversion of Biomass for Fuels Production
Himmel1, M. E., Baker, J. O., & Overend, R. P. (1994) · ACS symposium series · doi:10.1021/bk-1994-0566 · Cited by 405
Kuyper, M., HARTOG, M., Toirkens, M. J., Almering, M. J. H., Winkler, A. A., VANDIJKEN, J., & Pronk, J. T. (2004) · FEMS Yeast Research · doi:10.1016/j.femsyr.2004.09.010 · Cited by 404
Self-citationAndreasen, A. A., & Stier, T. J. B. (1954) · Journal of Cellular and Comparative Physiology · doi:10.1002/jcp.1030430303 · Cited by 392
Kuyper, M., Harhangi, H. R., STAVE, A., Winkler, A. A., Jetten, M. S. M., DELAAT, W., DENRIDDER, J., den Camp, H. J. M. O., VANDIJKEN, J., & Pronk, J. T. (2003) · FEMS Yeast Research · doi:10.1016/s1567-1356(03)00141-7 · Cited by 366
Self-citationEvolutionary engineering of mixed-sugar utilization by a xylose-fermenting strain
KUYPER, M., TOIRKENS, M., DIDERICH, J., WINKLER, A., VANDIJKEN, J., & PRONK, J. (2005) · FEMS Yeast Research · doi:10.1016/j.femsyr.2005.04.004 · Cited by 341
Self-citationEvolutionary Engineering of Industrially Important Microbial Phenotypes
Sauer, U. (2001) · Advances in biochemical engineering, biotechnology · doi:10.1007/3-540-45300-8_7 · Cited by 300
Minimal metabolic engineering of for efficient anaerobic xylose fermentation: a proof of principle
Kuyper, M., Winkler, A. A., VANDIJKEN, J., & Pronk, J. T. (2004) · FEMS Yeast Research · doi:10.1016/j.femsyr.2004.01.003 · Cited by 261
Self-citationA Modified Saccharomyces cerevisiae Strain That Consumes l -Arabinose and Produces Ethanol
Becker, J., & Boles, E. (2003) · Applied and Environmental Microbiology · doi:10.1128/aem.69.7.4144-4150.2003 · Cited by 253
Evolutionary Engineering of Saccharomyces cerevisiae for Anaerobic Growth on Xylose
Sonderegger, M., & Sauer, U. (2003) · Applied and Environmental Microbiology · doi:10.1128/aem.69.4.1990-1998.2003 · Cited by 251 · cited 1×
“The combination of metabolic and evolutionary engineering with the heterologous expression of either yeast xylose reductase and xylitol dehydrogenase (14, 32, 34, 35, 41) or a fungal xylose isomerase (19–22) has already enabled the anaerobic fermentation of D-xylose by S.”
BackgroundToivari, M. H., Aristidou, A. A., Ruohonen, L., & Penttilä, M. E. (2001) · Metabolic Engineering · doi:10.1006/mben.2000.0191 · Cited by 248
Reproducibility of Oligonucleotide Microarray Transcriptome Analyses
Piper, M. D. W., Daran-Lapujade, P. A. S., Bro, C., Regenberg, B., Knudsen, S., Nielsen, J., & Pronk, J. T. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m204490200 · Cited by 236
Self-citationA theoretical evaluation of growth yields of yeasts
Verduyn, C., Stouthamer, A. H., Scheffers, W. A., & van Dijken, J. P. (1991) · Antonie van Leeuwenhoek · doi:10.1007/bf00582119 · Cited by 236
Verho, R., Londesborough, J., Penttilä, M. E., & Richard, P. (2003) · Applied and Environmental Microbiology · doi:10.1128/aem.69.10.5892-5897.2003 · Cited by 227
Sedlák, M., & Ho, N. W. Y. (2004) · Applied Biochemistry and Biotechnology · doi:10.1385/abab:114:1-3:403 · Cited by 199
Jeppsson, M., Johansson, B., Hahn‐Hägerdal, B., & Gorwa‐Grauslund, M. F. (2002) · Applied and Environmental Microbiology · doi:10.1128/aem.68.4.1604-1609.2002 · Cited by 186
Jin, Y., Ni, H., Laplaza, J. M., & Jeffries, T. W. (2003) · Applied and Environmental Microbiology · doi:10.1128/aem.69.1.495-503.2003 · Cited by 181
Urk, H. V., Mark, P. R., Scheffers, W. A., & van Dijken, J. P. (1988) · Yeast · doi:10.1002/yea.320040406 · Cited by 159
Karhumaa, K., Wiedemann, B., Hahn‐Hägerdal, B., Boles, E., & Gorwa‐Grauslund, M. F. (2006) · Microbial Cell Factories · doi:10.1186/1475-2859-5-18 · Cited by 149
Jansen, M. L. A., Diderich, J. A., Mashego, M., Hassane, A., de Winde, J. H., Daran-Lapujade, P., & Pronk, J. T. (2005) · Microbiology · doi:10.1099/mic.0.27577-0 · Cited by 126
Self-citationProduction of ethanol from -arabinose by containing a fungal -arabinose pathway
Richard, P., Verho, R., PUTKONEN, M., Londesborough, J., & Penttilä, M. E. (2003) · FEMS Yeast Research · doi:10.1016/s1567-1356(02)00184-8 · Cited by 120
Weusthuis, R. A., Visser, W. F., Pronk, J. T., Scheffers, W. A., & van Dijken, J. P. (1994) · Microbiology · doi:10.1099/00221287-140-4-703 · Cited by 106
Self-citationChanges in the metabolome of associated with evolution in aerobic glucose-limited chemostats
Mashego, M. R., Jansen, M. L. A., Vinke, J. L., VANGULIK, W., & Heijnen, J. J. (2004) · FEMS Yeast Research · doi:10.1016/j.femsyr.2004.11.008 · Cited by 98
Pentose Fermentation by Lactobacillus plantarum
DOMAGK, G. F., & Horecker, B. L. (1958) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64750-7 · Cited by 86
Arabinose utilization by xylose-fermenting yeasts and fungi
McMillan, J. D., & Boynton, B. L. (1994) · Applied Biochemistry and Biotechnology · doi:10.1007/bf02941831 · Cited by 86
Kou, S.-C., Christensen, M. S., & Cirillo, V. P. (1970) · Journal of Bacteriology · doi:10.1128/jb.103.3.671-678.1970 · Cited by 83
PENTOSE FERMENTATION BY LACTOBACILLUS PLANTARUM
Heath, E. C., Horecker, B. L., Smyrniotis, P. Z., & Takagi, Y. (1958) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)70464-x · Cited by 71
Sedlák, M., & Ho, N. W. Y. (2001) · Enzyme and Microbial Technology · doi:10.1016/s0141-0229(00)00282-9 · Cited by 66
Screening for L-arabinose fermenting yeasts.
Dien, B. S., Kurtzman, C. P., Badal Chandra, S. A. H. A., & Bothast, R. J. (1996) · PubMed · Cited by 64
Patrick, J., & Lee, N. (1975) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(75)41098-9 · Cited by 39
Candida arabinofermentans, a new L-arabinose fermenting yeast
Kurtzman, C. P., & Dien, B. S. (1998) · Antonie van Leeuwenhoek · doi:10.1023/a:1001799607871 · Cited by 33
Grohmann, K., & Rj, B. (1994) · ACS Symposium series. American Chemical Society · Cited by 27
Cited by
Sorted by citations
Hemicelluloses for fuel ethanol: A review
Gírio, F. M., Fonseca, C., Carvalheiro, F., Duarte, L. C., Marques, S., & Bogel‐Łukasik, R. (2010) · Bioresource Technology · doi:10.1016/j.biortech.2010.01.088 · Cited by 1,648
Trends in bioconversion of lignocellulose: Biofuels, platform chemicals & biorefinery concept
Menon, V., & Rao, M. (2012) · Progress in Energy and Combustion Science · doi:10.1016/j.pecs.2012.02.002 · Cited by 1,631
Adaptive laboratory evolution—Principles and applications for biotechnology
Dragosits, M., & Mattanovich, D. (2013) · Microbial Cell Factories · doi:10.1186/1475-2859-12-64 · Cited by 827
Technological trends, global market, and challenges of bio-ethanol production
Mussatto, S. I., Dragone, G., Guimarães, P. M. R., Silva, J. P. A., Carneiro, L. M., Roberto, I. C., Vicente, A. A., Domingues, L., & Teixeira, J. A. (2010) · Biotechnology Advances · doi:10.1016/j.biotechadv.2010.07.001 · Cited by 730
Progress in Metabolic Engineering of Saccharomyces cerevisiae
Nevoigt, E. (2008) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00025-07 · Cited by 597
Metadata
- Authors
- Wisselink, H. W., Toirkens, M. J., del Rosario Franco Berriel, M., Winkler, A. A., van Dijken, J. P., Pronk, J. T., & van Maris, A. J. A.
- Affiliations
- Delft University of Technology (NL)
- Bird Engineering B.V., Westfrankelandsedijk 1, 3115 HG Schiedam, The Netherlands
- Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft
- ORCID
- Pronk, Jack T.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2007-08
- Volume
- 73
- Issue
- 15
- Pages
- 4881-4891
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- PMC
- PMC1951023
- OpenAlex
- W2139956564
- Cited by
- 209
- References
- 46
- Citations by year
- 2026: 3 · 2025: 7 · 2024: 3 · 2023: 5 · 2022: 6 · 2021: 6 · 2020: 11 · 2019: 12 · 2018: 13 · 2017: 11 · 2016: 11 · 2015: 10 · 2014: 9 · 2013: 12 · 2012: 16
- FWCI
- 12.19
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Applied and Environmental Microbiology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Research Repository (Delft University of Technology) (submittedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion, other-oa)
- Funders
- Ministerie van Economische Zaken
- Nederlandse Organisatie voor Wetenschappelijk Onderzoek
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Keywords
- Saccharomyces cerevisiae, ethanol production, evolutionary engineering, lignocellulosic biomass, xylose fermentation, heterologous expression
- MeSH terms
- Ethanol, Anaerobiosis, Arabinose, Fermentation, Genetic Engineering, Industrial Microbiology, Pentose Phosphate Pathway, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Lactiplantibacillus plantarum
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
