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Cited by 141Phytoestrogen effects and researchGut microbiota and healthBiochemical and biochemical processes

The DOI matches its Crossref record; 56 of the 57 references it cites were found in a registry or an index.

Conversion of Daidzein and Genistein by an Anaerobic Bacterium Newly Isolated from the Mouse Intestine

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Matthies, Anastasia, Clavel, Thomas, Gütschow, Michael, Engst, Wolfram, Haller, Dirk, Blaut, Michael, Braune, Annett·2008·Applied and Environmental Microbiology
DOI 10.1128/aem.00555-08PMC PMC2519357
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Abstract

The metabolism of isoflavones by gut bacteria plays a key role in the availability and bioactivation of these compounds in the intestine. Daidzein and genistein are the most common dietary soy isoflavones. While daidzein conversion yielding equol has been known for some time, the corresponding formation of 5-hydroxy-equol from genistein has not been reported previously. We isolated a strictly anaerobic bacterium (Mt1B8) from the mouse intestine which converted daidzein via dihydrodaidzein to equol as well as genistein via dihydrogenistein to 5-hydroxy-equol. Strain Mt1B8 was a gram-positive, rod-shaped bacterium identified as a member of the Coriobacteriaceae. Strain Mt1B8 also transformed dihydrodaidzein and dihydrogenistein to equol and 5-hydroxy-equol, respectively. The conversion of daidzein, genistein, dihydrodaidzein, and dihydrogenistein in the stationary growth phase depended on preincubation with the corresponding isoflavonoid, indicating enzyme induction. Moreover, dihydrogenistein was transformed even more rapidly in the stationary phase when strain Mt1B8 was grown on either genistein or daidzein. Growing the cells on daidzein also enabled conversion of genistein. This suggests that the same enzymes are involved in the conversion of the two isoflavones.

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Metadata

Authors
Matthies, A., Clavel, T., Gütschow, M., Engst, W., Haller, D., Blaut, M., & Braune, A.
Affiliations
  • German Institute of Human Nutrition (DE)
  • Technical University of Munich (DE)
  • Pharmaceutical Institute, Pharmaceutical Chemistry I, University of Bonn, An der Immenburg 4, D-53121 Bonn, Germany
  • Department of Gastrointestinal Microbiology
Corresponding author
Braune, Annett
ORCID
Venue
Applied and Environmental Microbiology
Publisher
American Society for Microbiology
Published
2008-08
Volume
74
Issue
15
Pages
4847-4852
ISSN
  • 0099-2240 (ISSN-L)
  • 1098-5336
DOI
10.1128/aem.00555-08
PMC
PMC2519357
OpenAlex
W2067334985
Cited by
141
References
57
Citations by year
2026: 7 · 2025: 4 · 2024: 16 · 2023: 9 · 2022: 15 · 2021: 9 · 2020: 10 · 2019: 11 · 2018: 5 · 2017: 5 · 2016: 6 · 2015: 8 · 2014: 5 · 2013: 8 · 2012: 6
FWCI
4.02
Type
journal article
Language
en
Open access
Licence
journals.asm.org/non-commercial-tdm-license (tdm)
Locations
  • Applied and Environmental Microbiology (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
Deutsche Forschungsgemeinschaft
Topics
  • Health Sciences › Nursing › Nutrition and Dietetics › Phytoestrogen effects and research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Biochemical and biochemical processes
Keywords
daidzein, genistein, isoflavone metabolism, equol, dihydrodaidzein, gut microbiota, Coriobacteriaceae, anaerobic bacteria, enzyme induction, soy isoflavones, mouse intestine, metabolic activation
MeSH terms
Animals, Bacteria, Anaerobic, Biotransformation, Chromatography, High Pressure Liquid, Female, Gram-Positive Bacteria, Ileum, Isoflavones, Mice, Inbred C57BL, Molecular Sequence Data, Genistein, Spectrometry, Mass, Electrospray Ionization, Mice

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