High-Level Production of the Low-Calorie Sugar Sorbitol by Lactobacillus plantarum through Metabolic Engineering
Open accessAbstract
Sorbitol is a low-calorie sugar alcohol that is largely used as an ingredient in the food industry, based on its sweetness and its high solubility. Here, we investigated the capacity of Lactobacillus plantarum , a lactic acid bacterium found in many fermented food products and in the gastrointestinal tract of mammals, to produce sorbitol from fructose-6-phosphate by reverting the sorbitol catabolic pathway in a mutant strain deficient for both l - and d -lactate dehydrogenase activities. The two sorbitol-6-phosphate dehydrogenase (Stl6PDH) genes ( srlD1 and srlD2 ) identified in the genome sequence were constitutively expressed at a high level in this mutant strain. Both Stl6PDH enzymes were shown to be active, and high specific activity could be detected in the overexpressing strains. Using resting cells under pH control with glucose as a substrate, both Stl6PDHs were capable of rerouting the glycolytic flux from fructose-6-phosphate toward sorbitol production with a remarkably high efficiency (61 to 65% glucose conversion), which is close to the maximal theoretical value of 67%. Mannitol production was also detected, albeit at a lower level than the control strain (9 to 13% glucose conversion), indicating competition for fructose-6-phosphate rerouting by natively expressed mannitol- 1 -phosphate dehydrogenase. By analogy, low levels of this enzyme were detected in both the wild-type and the lactate dehydrogenase-deficient strain backgrounds. After optimization, 25% of sugar conversion into sorbitol was achieved with cells grown under pH control. The role of intracellular NADH pools in the determination of the maximal sorbitol production is discussed.
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Sugar fermentations to produce polyols such as sorbitol, mannitol, xylitol are feasible although they cannot yet compete with chemical reduction economically.
“Sugar fermentations to produce these polyols are also feasible although it cannot yet compete with the chemical reduction economically.[149][150][151]”
From Catalysis as an Enabling Science for Sustainable Polymers (2018) · cites this paper as [151]
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- Authors
- Ladero, V., Ramos, A., Wiersma, A., Goffin, P., Schanck, A., Kleerebezem, M., Hugenholtz, J., Smid, E. J., & Hols, P.
- Affiliations
- Unité de Génétique, Institut des Sciences de la Vie, Université catholique de Louvain, 1348 Louvain-la-Neuve, Belgium
- Instituto de Biologia Experimental e Tecnológica (PT)
- Nederlands Instituut Voor Zuivel Oonderzoek (NL)
- UCLouvain (BE)
- Laboratoire de Chimie Physique et de Cristallographie, Université catholique de Louvain, Place Louis Pasteur 1, 1348 Louvain-la-Neuve, Belgium
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2007-03-15
- Volume
- 73
- Issue
- 6
- Pages
- 1864-1872
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- PMC
- PMC1828817
- OpenAlex
- W2112476663
- Cited by
- 127
- References
- 41
- Citations by year
- 2026: 3 · 2025: 6 · 2024: 7 · 2023: 4 · 2022: 9 · 2021: 8 · 2020: 3 · 2019: 9 · 2018: 11 · 2017: 11 · 2016: 13 · 2015: 4 · 2014: 6 · 2013: 7 · 2012: 7
- FWCI
- 2.25
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Applied and Environmental Microbiology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- UvA-DARE (University of Amsterdam) (publishedVersion)
- Digital Access to Libraries (submittedVersion, other-oa)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
- Keywords
- sorbitol production, Lactobacillus plantarum, sorbitol-6-phosphate dehydrogenase, glycolytic flux, mannitol-1-phosphate dehydrogenase, mannitol production, sugar alcohols, metabolic engineering
- MeSH terms
- Bacterial Proteins, Carbohydrates, Fermentation, Fructosephosphates, Genetic Engineering, Glucose, Hydrogen-Ion Concentration, L-Lactate Dehydrogenase, Mannitol, Mutation, Magnetic Resonance Spectroscopy, Sorbitol, Sugar Alcohol Dehydrogenases, Gene Expression, Lactiplantibacillus plantarum, Metabolic Networks and Pathways
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