Metabolic response of Clostridium ljungdahlii to oxygen exposure

Whitham, Jason M.; Tirado-Acevedo, Oscar; Chinn, Mari S.; Pawlak, Joel J.; Grunden, Amy M.

doi:10.1128/aem.02491-15

Cited by 47Microbial Metabolic Engineering and BioproductionBiofuel production and bioconversionAnaerobic Digestion and Biogas Production

Metabolic response of Clostridium ljungdahlii to oxygen exposure

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Whitham, Jason M., Tirado-Acevedo, Oscar, Chinn, Mari S., Pawlak, Joel J., Grunden, Amy M.·2015·Applied and Environmental Microbiology

DOI 10.1128/aem.02491-15PMC PMC4644658

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Abstract

Clostridium ljungdahlii is an important synthesis gas-fermenting bacterium used in the biofuels industry, and a preliminary investigation showed that it has some tolerance to oxygen when cultured in rich mixotrophic medium. Batch cultures not only continue to grow and consume H2, CO, and fructose after 8% O2 exposure, but fermentation product analysis revealed an increase in ethanol concentration and decreased acetate concentration compared to non-oxygen-exposed cultures. In this study, the mechanisms for higher ethanol production and oxygen/reactive oxygen species (ROS) detoxification were identified using a combination of fermentation, transcriptome sequencing (RNA-seq) differential expression, and enzyme activity analyses. The results indicate that the higher ethanol and lower acetate concentrations were due to the carboxylic acid reductase activity of a more highly expressed predicted aldehyde oxidoreductase (CLJU_c24130) and that C. ljungdahlii's primary defense upon oxygen exposure is a predicted rubrerythrin (CLJU_c39340). The metabolic responses of higher ethanol production and oxygen/ROS detoxification were found to be linked by cofactor management and substrate and energy metabolism. This study contributes new insights into the physiology and metabolism of C. ljungdahlii and provides new genetic targets to generate C. ljungdahlii strains that produce more ethanol and are more tolerant to syngas contaminants.

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C. ljungdahlii has been shown to detoxify O2 and ROS, likely via rubrerythrin and hydrogen peroxidases.
“C. ljungdahlii has been shown to detoxify O2 and ROS (likely via rubrerythrin and hydrogen peroxidases) and ethanol formation could actually be stimulated by exposure to O2 (likely due to changes in AOR activity and co-factor metabolism) (Whitham et al., 2015).”
From Gas Fermentation—A Flexible Platform for Commercial Scale Production of Low-Carbon-Fuels and Chemicals from Waste and Renewable Feedstocks (2016) · cites this paper as Whitham et al., 2015
Ethanol formation in C. ljungdahlii could be stimulated by exposure to O2, likely due to changes in AOR activity and co-factor metabolism.
“C. ljungdahlii has been shown to detoxify O2 and ROS (likely via rubrerythrin and hydrogen peroxidases) and ethanol formation could actually be stimulated by exposure to O2 (likely due to changes in AOR activity and co-factor metabolism) (Whitham et al., 2015).”
From Gas Fermentation—A Flexible Platform for Commercial Scale Production of Low-Carbon-Fuels and Chemicals from Waste and Renewable Feedstocks (2016) · cites this paper as Whitham et al., 2015

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The deletion of aldehyde:ferredoxin oxidoreductase-encoding genes in Clostridium ljungdahlii results in changes in the product spectrum with various carbon sourcesPreprint
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Physiological response of Clostridium ljungdahlii DSM 13528 of ethanol production under different fermentation conditions
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Authors: Whitham, J. M., Tirado-Acevedo, O., Chinn, M. S., Pawlak, J. J., & Grunden, A. M.
Affiliations: North Carolina State University (US)
ORCID: Whitham, Jason M.
Chinn, Mari S.
Pawlak, Joel J.
Grunden, Amy M.
Venue: Applied and Environmental Microbiology
Publisher: American Society for Microbiology
Published: 2015-12-15
Volume: 81
Issue: 24
Pages: 8379-8391
ISSN: 0099-2240 (ISSN-L)
1098-5336
DOI: 10.1128/aem.02491-15
PMC: PMC4644658
OpenAlex: W2288696301
Cited by: 47
References: 111
Citations by year: 2026: 1 · 2025: 4 · 2024: 2 · 2023: 4 · 2022: 5 · 2021: 9 · 2020: 3 · 2019: 5 · 2018: 2 · 2017: 6 · 2016: 6
FWCI: 2.02
Type: journal article
Language: en
Open access: bronze
doi.org
Licence: journals.asm.org/non-commercial-tdm-license (tdm)
Locations: Applied and Environmental Microbiology (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
Physical Sciences › Engineering › Building and Construction › Anaerobic Digestion and Biogas Production
Keywords: Clostridium ljungdahlii, oxygen tolerance, reactive oxygen species scavenging, ethanol production, acetate production, carboxylic acid reductase, aldehyde oxidoreductase, rubrerythrin, energy metabolism, syngas fermentation, transcriptome, strain engineering
MeSH terms: Acetates, Ethanol, Aldehyde Oxidoreductases, Base Sequence, Carbon Dioxide, Clostridium, DNA, Bacterial, Energy Metabolism, Fermentation, Molecular Sequence Data, Oxidation-Reduction, Oxidoreductases, Oxygen, Gene Expression, Sequence Alignment, Reactive Oxygen Species, Sequence Analysis, DNA, Bioreactors, Biofuels

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Metabolic response of Clostridium ljungdahlii to oxygen exposure

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