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Cited by 127Oral microbiology and periodontitis researchBacterial biofilms and quorum sensingBacterial Genetics and Biotechnology

A Highly Arginolytic Streptococcus Species That Potently Antagonizes Streptococcus mutans

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Huang, Xuelian, Palmer, Sara R., Ahn, Sang-Joon, Richards, Vincent P., Williams, Matthew L., Nascimento, Marcelle M., Burne, Robert A.·2016·Applied and Environmental Microbiology
DOI 10.1128/aem.03887-15PMC PMC4807514
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Abstract

The ability of certain oral biofilm bacteria to moderate pH through arginine metabolism by the arginine deiminase system (ADS) is a deterrent to the development of dental caries. Here, we characterize a novel Streptococcus strain, designated strain A12, isolated from supragingival dental plaque of a caries-free individual. A12 not only expressed the ADS pathway at high levels under a variety of conditions but also effectively inhibited growth and two intercellular signaling pathways of the dental caries pathogen Streptococcus mutans . A12 produced copious amounts of H 2 O 2 via the pyruvate oxidase enzyme that were sufficient to arrest the growth of S. mutans . A12 also produced a protease similar to challisin (Sgc) of Streptococcus gordonii that was able to block the competence-stimulating peptide (CSP)–ComDE signaling system, which is essential for bacteriocin production by S. mutans . Wild-type A12, but not an sgc mutant derivative, could protect the sensitive indicator strain Streptococcus sanguinis SK150 from killing by the bacteriocins of S. mutans . A12, but not S. gordonii , could also block the XIP ( com X - i nducing p eptide) signaling pathway, which is the proximal regulator of genetic competence in S. mutans , but Sgc was not required for this activity. The complete genome sequence of A12 was determined, and phylogenomic analyses compared A12 to streptococcal reference genomes. A12 was most similar to Streptococcus australis and Streptococcus parasanguinis but sufficiently different that it may represent a new species. A12-like organisms may play crucial roles in the promotion of stable, health-associated oral biofilm communities by moderating plaque pH and interfering with the growth and virulence of caries pathogens.

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Crossref · 10.1128/aem.03887-15

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  • The novel Streptococcus strain A12 strongly expressed the arginine deaminase pathway and produced hydrogen peroxide in large quantities along with specific virulence factors that could arrest S mutans growth and interrupt S mutans signaling pathways.

    “In addition to strongly expressing the arginine deaminase pathway, the novel strain of Streptococcus, called A12, produced hydrogen peroxide in large quantities along with specific virulence factors that could arrest S mutans growth and interrupt S mutans signaling pathways.”

    From The oral microbiome: Role of key organisms and complex networks in oral health and disease (2021) · cites this paper as [45]

  • A12 demonstrated the capacity to prevent S mutans from killing other arginine deiminase system producers by degrading the S mutans bacteriocin-inducing peptide signal through secreting a protease that blocked the competence-stimulating peptide signaling system.

    “A12 also demonstrated the capacity to prevent S mutans from killing other arginine deiminase system (ADS) producers by degrading the S mutans bacteriocin‐inducing peptide signal— accomplished through secreting a protease that blocked the competence‐stimulating peptide signaling system.”

    From The oral microbiome: Role of key organisms and complex networks in oral health and disease (2021) · cites this paper as [45]

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Authors
Huang, X., Palmer, S. R., Ahn, S.-J., Richards, V. P., Williams, M. L., Nascimento, M. M., & Burne, R. A.
Affiliations
  • University of Florida (US)
  • Clemson University (US)
  • University of Florida Health (US)
ORCID
Venue
Applied and Environmental Microbiology
Publisher
American Society for Microbiology
Published
2016-04
Volume
82
Issue
7
Pages
2187-2201
ISSN
  • 0099-2240 (ISSN-L)
  • 1098-5336
DOI
10.1128/aem.03887-15
PMC
PMC4807514
OpenAlex
W2315957845
Cited by
127
References
72
Citations by year
2026: 4 · 2025: 13 · 2024: 10 · 2023: 11 · 2022: 10 · 2021: 12 · 2020: 14 · 2019: 24 · 2018: 12 · 2017: 14 · 2016: 3
FWCI
26.36
Type
journal article
Language
en
Open access
Licence
journals.asm.org/non-commercial-tdm-license (tdm)
Locations
  • Applied and Environmental Microbiology (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • HHS | NIH | National Institute of Dental and Craniofacial Research (R90 DE22530, K23 DE023579)
  • Colgate-Palmolive Company
Topics
  • Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords
Streptococcus, arginine deiminase system, arginine metabolism, Streptococcus mutans, dental caries, antagonism, supragingival plaque, hydrogen peroxide, pyruvate oxidase, proteolytic enzymes, competence-stimulating peptide, bacteriocin production, genetic competence, Streptococcus sanguinis, Streptococcus australis, Streptococcus parasanguinis, new species, dental plaque, plaque pH
MeSH terms
Antibiosis, Arginine, Bacterial Proteins, Bacteriocins, Dental Plaque, Phylogeny, Streptococcus, Streptococcus mutans, Gene Expression Regulation, Bacterial, Biofilms

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