Development of a Sensitive Gene Expression Reporter System and an Inducible Promoter-Repressor System for Clostridium acetobutylicum
Open accessAbstract
A sensitive gene expression reporter system was developed for Clostridium acetobutylicum ATCC 824 by using a customized gusA expression cassette. In discontinuous cultures, time course profiles of beta-glucuronidase specific activity reflected adequately in vivo dynamic up- and down-regulation of acidogenesis- and/or solventogenesis-associated promoter expression in C. acetobutylicum. Furthermore, a new inducible gene expression system was developed in C. acetobutylicum, based on the Staphylococcus xylosus xylose operon promoter-repressor regulatory system.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Gene expression reporter systems that function under anaerobic conditions have emerged, including the β-glucuronidase gene, chloramphenicol acetyltransferase gene, flavin-based fluorescent proteins, luxA-luxB reporter system, and lacZ gene from Thermoanaerobacterium thermosulfurogenes EM1.
“Gene expression reporter systems that function under anaerobic conditions have emerged, including the β-glucuronidase gene (gusA) (Girbal et al., 2003; Dong et al., 2012; Banerjee et al., 2014), chloramphenicol acetyltransferase gene (catP) (Heap et al., 2007; Zhang et al., 2015b), flavin-based fluorescent proteins (Drepper et al., 2007, 2010; Mukherjee and Schroeder, 2015), luxA-luxB reporter”
From Gas Fermentation—A Flexible Platform for Commercial Scale Production of Low-Carbon-Fuels and Chemicals from Waste and Renewable Feedstocks (2016) · cites this paper as Girbal et al., 2003
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
GUS fusions: beta‐glucuronidase as a sensitive and versatile gene fusion marker in higher plants.
Jefferson, R., Kavanagh, T. A., & Bevan, M. W. (1987) · The EMBO Journal · doi:10.1002/j.1460-2075.1987.tb02730.x · Cited by 9,854 · see citing works
beta-Glucuronidase from Escherichia coli as a gene-fusion marker.
Jefferson, R., Burgess, S. M., & Hirsh, D. (1986) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.83.22.8447 · Cited by 992 · see citing works
Nölling, J., Breton, G., Omelchenko, M. V., Makarova, K. S., Zeng, Q., Gibson, R., Lee, H. M., Dubois, J., Qiu, D., Hitti, J., et al. (2001) · Journal of Bacteriology · doi:10.1128/jb.183.16.4823-4838.2001 · Cited by 767 · see citing works
Self-citationJefferson, R. (1989) · Nature · doi:10.1038/342837a0 · Cited by 518 · see citing works
Mermelstein, L. D., & Papoutsakis, E. T. (1993) · Applied and Environmental Microbiology · doi:10.1128/aem.59.4.1077-1081.1993 · Cited by 291 · see citing works
Expression of cloned homologous fermentative genes in Clostridium acetobutylicum ATCC 824
Mermelstein, L. D., Welker, N. E., Bennett, G. N., & Papoutsakis, E. T. (1992) · Nature Biotechnology · doi:10.1038/nbt0292-190 · Cited by 249 · see citing works
Harris, L. M., Welker, N. E., & Papoutsakis, E. T. (2002) · Journal of Bacteriology · doi:10.1128/jb.184.13.3586-3597.2002 · Cited by 230 · see citing works
Vasconcelos, I., Girbal, L., & Soucaille, P. (1994) · Journal of Bacteriology · doi:10.1128/jb.176.5.1443-1450.1994 · Cited by 228 · see citing works
Self-citationSpo0A directly controls the switch from acid to solvent production in solvent‐forming clostridia
Ravagnani, A., Jennert, K. C. B., Steiner, E., Grünberg, R., Jefferies, J. R., Wilkinson, S. R., Young, D. I., Tidswell, E. C., Brown, D. P., Youngman, P. J., et al. (2000) · Molecular Microbiology · doi:10.1046/j.1365-2958.2000.02071.x · Cited by 146 · see citing works
Tummala, S. B., Welker, N. E., & Papoutsakis, E. T. (1999) · Applied and Environmental Microbiology · doi:10.1128/aem.65.9.3793-3799.1999 · Cited by 130 · see citing works
Gorwa‐Grauslund, M. F., Croux, C., & Soucaille, P. (1996) · Journal of Bacteriology · doi:10.1128/jb.178.9.2668-2675.1996 · Cited by 109 · see citing works
Self-citationSizemore, C. F., Buchner, E., Rygus, T., Witke, C., Götz, F., & Hillen, W. (1991) · Molecular and General Genetics MGG · doi:10.1007/bf00273926 · Cited by 94 · see citing works
Walter, K. A., Nair, R. V., Cary, J. W., Bennett, G. N., & Papoutsakis, E. T. (1993) · Gene · doi:10.1016/0378-1119(93)90182-3 · Cited by 80 · see citing works
Wieland, K.-P., Wieland, B., & Götz, F. (1995) · Gene · doi:10.1016/0378-1119(95)00137-u · Cited by 77 · see citing works
Control of Butanol Formation in Clostridium acetobutylicum by Transcriptional Activation
Thormann, K. M., Feustel, L., Lorenz, K., Nakotte, S., & Dürre, P. (2002) · Journal of Bacteriology · doi:10.1128/jb.184.7.1966-1973.2002 · Cited by 75 · see citing works
Regulation of Staphylococcus xylosus xylose utilization genes at the molecular level
Sizemore, C. F., Wieland, B., Götz, F., & Hillen, W. (1992) · Journal of Bacteriology · doi:10.1128/jb.174.9.3042-3048.1992 · Cited by 52 · see citing works
Transcriptional regulation of solventogenesis in Clostridium acetobutylicum.
Dürre, P., Böhringer, M., Nakotte, S., Schaffer, S., Thormann, K. M., & Zickner, B. (2002) · PubMed · Cited by 52 · see citing works
Steffen, C., & Matzura, H. (1989) · Gene · doi:10.1016/0378-1119(89)90282-5 · Cited by 47 · see citing works
A Clostridium perfringens Vector for the Selection of Promoters
Matsushita, C., Matsushita, O., Koyama, M., & Okabe, A. (1994) · Plasmid · doi:10.1006/plas.1994.1035 · Cited by 36 · see citing works
Differential regulation of two thiolase genes from Clostridium acetobutylicum DSM 792.
Winzer, K., Lorenz, K., Zickner, B., & Dürre, P. (2000) · PubMed · Cited by 32 · see citing works
Girbal, L., Vasconcelos, I., & Soucaille, P. (1994) · Journal of Bacteriology · doi:10.1128/jb.176.19.6146-6147.1994 · Cited by 24 · see citing works
Self-citationPhillips‐Jones, M. K. (2000) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.2000.tb09164.x · Cited by 24 · see citing works
Quixley, K. W. M., & Reid, S. J. (2000) · PubMed · Cited by 20 · see citing works
Insulin dominantly suppresses hepatitis B virus gene expression in cultured human hepatoma cells
Chen, M., Lin, H.-M., & Chou, C. (1997) · Journal of Biomedical Science · doi:10.1007/bf02258353 · Cited by 12 · see citing works
Flagged first, then by citations
Research works printed but not linked: 1
- [15]Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
Cited by
Sorted by citations
Fermentative butanol production by clostridia
Lee, S. Y., Park, J. H., Jang, S. H., Nielsen, L. K., Kim, J. H., & Jung, K. S. (2008) · Biotechnology and Bioengineering · doi:10.1002/bit.22003 · Cited by 1,033 · see citing works
The ClosTron: a universal gene knock-out system for the genus Clostridium
Heap, J. T., Pennington, O. J., Cartman, S. T., Carter, G. P., & Minton, N. P. (2007) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2007.05.021 · Cited by 663 · see citing works
A modular system for Clostridium shuttle plasmids
Heap, J. T., Pennington, O. J., Cartman, S. T., & Minton, N. P. (2009) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2009.05.004 · Cited by 489 · see citing works
Liew, F., Martin, M. E., Tappel, R. C., Heijstra, B. D., Mihalcea, C., & Köpke, M. (2016) · Frontiers in Microbiology · doi:10.3389/fmicb.2016.00694 · Cited by 485 · see citing works
Commercial Biomass Syngas Fermentation.
Daniell, J., Köpke, M., & Simpson, S. (2012) · Energies · doi:10.3390/en5125372 · Cited by 444 · see citing works
Tracy, B. P., Jones, S. W., Fast, A. G., Indurthi, D. C., & Papoutsakis, E. T. (2011) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2011.10.008 · Cited by 408 · see citing works
Engineering solventogenic clostridia
Papoutsakis, E. T. (2008) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2008.08.003 · Cited by 332 · see citing works
Problems with the microbial production of butanol
Zheng, Y., Li, L., Xian, M., Ma, Y.-J., Yang, J., Xu, X., & He, D.-Z. (2009) · Journal of Industrial Microbiology & Biotechnology · doi:10.1007/s10295-009-0609-9 · Cited by 290 · see citing works
Huang, H., Chai, C., Li, N., Rowe, P., Minton, N. P., Yang, S., Jiang, W., & Gu, Y. (2016) · ACS Synthetic Biology · doi:10.1021/acssynbio.6b00044 · Cited by 200 · see citing works
Dürre, P. (2005) · CRC Press eBooks · doi:10.1201/9780203489819 · Cited by 178 · see citing works
Dusséaux, S., Croux, C., Soucaille, P., & Meynial‐Salles, I. (2013) · Metabolic Engineering · doi:10.1016/j.ymben.2013.03.003 · Cited by 157 · see citing works
Self-citationGirbal, L., von Abendroth, G., Winkler, M., Benton, P. M. C., Meynial‐Salles, I., Croux, C., Peters, J. W., Happe, T., & Soucaille, P. (2005) · Applied and Environmental Microbiology · doi:10.1128/aem.71.5.2777-2781.2005 · Cited by 139 · see citing works
Self-citationApproaches to genetic tool development for rapid domestication of non-model microorganisms
Riley, L. A., & Guss, A. M. (2021) · Biotechnology for Biofuels · doi:10.1186/s13068-020-01872-z · Cited by 127 · see citing works
Lactose-inducible system for metabolic engineering of Clostridium ljungdahlii
Banerjee, A., Leang, C., Ueki, T., Nevin, K. P., & Lovley, D. R. (2014) · Applied and Environmental Microbiology · doi:10.1128/aem.03666-13 · Cited by 126 · see citing works
Han, X. J., Li, Z., Jiang, Y., Yang, Y., Jiang, W., Gu, Y., & Yang, S. (2012) · Metabolic Engineering · doi:10.1016/j.ymben.2012.05.003 · Cited by 115 · see citing works
Recent Developments of the Synthetic Biology Toolkit for Clostridium
Joseph, R. C., Kim, N. M., & Sandoval, N. R. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.00154 · Cited by 109 · see citing works
The emergence of ‘hypervirulence’ in Clostridium difficile
Cartman, S. T., Heap, J. T., Kuehne, S. A., Cockayne, A., & Minton, N. P. (2010) · International Journal of Medical Microbiology · doi:10.1016/j.ijmm.2010.04.008 · Cited by 104 · see citing works
Hartman, A. H., Liu, H., & Melville, S. B. (2010) · Applied and Environmental Microbiology · doi:10.1128/aem.01536-10 · Cited by 94 · see citing works
VONABENDROTH, G., Stripp, S. T., Silakov, A., Croux, C., Soucaille, P., Girbal, L., & Happe, T. (2008) · International Journal of Hydrogen Energy · doi:10.1016/j.ijhydene.2008.07.122 · Cited by 92 · see citing works
Self-citationZhao, R., Liu, Y., Zhang, H., Chai, C., Wang, J., Jiang, W., & Gu, Y. (2019) · ACS Synthetic Biology · doi:10.1021/acssynbio.9b00033 · Cited by 82 · see citing works
Technical guide for genetic advancement of underdeveloped and intractable Clostridium
Pyne, M. E., Bruder, M., Moo-Young, M., Chung, D. A., & Chou, C. P. (2014) · Biotechnology Advances · doi:10.1016/j.biotechadv.2014.04.003 · Cited by 78 · see citing works
Application of new metabolic engineering tools for Clostridium acetobutylicum
Lütke‐Eversloh, T. (2014) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-014-5785-5 · Cited by 73 · see citing works
Mingardon, F., Chanal, A., Tardif, C., & Fierobe, H. (2011) · Applied and Environmental Microbiology · doi:10.1128/aem.03012-10 · Cited by 66 · see citing works
Dong, H., Tao, W., Zhang, Y., & Li, Y. (2012) · Metabolic Engineering · doi:10.1016/j.ymben.2011.10.004 · Cited by 65 · see citing works
Streett, H., Kalis, K. M., & Papoutsakis, E. T. (2019) · Applied and Environmental Microbiology · doi:10.1128/aem.00622-19 · Cited by 65 · see citing works
Efficient gene knockdown in Clostridium acetobutylicum by synthetic small regulatory RNAs
Cho, C., & Lee, S. Y. (2016) · Biotechnology and Bioengineering · doi:10.1002/bit.26077 · Cited by 58 · see citing works
Characterization and Development of Two Reporter Gene Systems for Clostridium acetobutylicum
Feustel, L., Nakotte, S., & Dürre, P. (2004) · Applied and Environmental Microbiology · doi:10.1128/aem.70.2.798-803.2004 · Cited by 55 · see citing works
Philipps, G., de Vries, S., & Jennewein, S. (2019) · Biotechnology for Biofuels · doi:10.1186/s13068-019-1448-1 · Cited by 54 · see citing works
Intrinsic Class D β-Lactamases of Clostridium difficile
Tóth, M., Stewart, N. K., Smith, C. A., & Vakulenko, S. B. (2018) · mBio · doi:10.1128/mbio.01803-18 · Cited by 53 · see citing works
Metabolic engineering of clostridia for the production of chemicals
Cho, C., Jang, Y., Moon, H. G., Lee, J., & Lee, S. Y. (2014) · Biofuels Bioproducts and Biorefining · doi:10.1002/bbb.1531 · Cited by 52 · see citing works
Agu, C. V., Ujor, V. C., & Ezeji, T. C. (2019) · Biotechnology for Biofuels · doi:10.1186/s13068-019-1388-9 · Cited by 45 · see citing works
A novel arabinose-inducible genetic operation system developed for Clostridium cellulolyticum
Zhang, J., Liu, Y., Cui, G., & Cui, Q. (2015) · Biotechnology for Biofuels · doi:10.1186/s13068-015-0214-2 · Cited by 32 · see citing works
Teng, L., Wang, K., Xu, J., & Xu, C. (2015) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2015.09.018 · Cited by 32 · see citing works
Isolation of Genetically Tractable Most-Wanted Bacteria by Metaparental Mating
Cuív, P. Ó., Smith, W. J. M., Pottenger, S., Burman, S., Shanahan, E. R., & Morrison, M. (2015) · Scientific Reports · doi:10.1038/srep13282 · Cited by 31 · see citing works
Low-Carbon Fuel and Chemical Production by Anaerobic Gas Fermentation
Daniell, J., Nagaraju, S., Burton, F., Köpke, M., & Simpson, S. D. (2015) · Advances in biochemical engineering, biotechnology · doi:10.1007/10_2015_5005 · Cited by 28 · see citing works
Streett, H., Charubin, K., & Papoutsakis, E. T. (2021) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2021.07.005 · Cited by 28 · see citing works
Building a genome engineering toolbox in nonmodel prokaryotic microbes
Freed, E. F., Fenster, J. A., Smolinski, S. L., Walker, J. E., Henard, C. A., Gill, R. T., & Eckert, C. A. (2018) · Biotechnology and Bioengineering · doi:10.1002/bit.26727 · Cited by 24 · see citing works
Seo, S.-O., Lu, T., Jin, Y., & Blaschek, H. P. (2017) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2017.11.003 · Cited by 21 · see citing works
Part by Part: Synthetic Biology Parts Used in Solventogenic Clostridia
Gyulev, I. S., Willson, B. J., Hennessy, R. C., Krabben, P., Jenkinson, E. R., & Thomas, G. H. (2017) · ACS Synthetic Biology · doi:10.1021/acssynbio.7b00327 · Cited by 22 · see citing works
Köhler, K. A. K., Rühl, J., Blank, L. M., & Schmid, A. (2014) · Engineering in Life Sciences · doi:10.1002/elsc.201400041 · Cited by 20 · see citing works
Methanoferrodoxin represents a new class of superoxide reductase containing an iron–sulfur cluster
Krätzer, C., Welte, C. U., Dörner, K., Friedrich, T., & Deppenmeier, U. (2010) · FEBS Journal · doi:10.1111/j.1742-4658.2010.07964.x · Cited by 19 · see citing works
Lee, J., Jang, Y., Papoutsakis, E. T., & Lee, S. Y. (2016) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2016.05.020 · Cited by 16 · see citing works
Interaction between the breast tumor microenvironment and gut microbiome
Tang, G., Millar, E. K. A., Graham, P., & Beretov, J. (2025) · Gut Microbes · doi:10.1080/19490976.2025.2514136 · Cited by 13 · see citing works
Biobutanol Production From Renewable Resources
Zhang, J., Wang, S., & Wang, Y. (2016) · Advances in bioenergy · doi:10.1016/bs.aibe.2016.09.001 · Cited by 12 · see citing works
Zhang, Y., Bailey, T. S., Kubiak, A. M., Lambin, P., & Theys, J. (2022) · ACS Synthetic Biology · doi:10.1021/acssynbio.2c00401 · Cited by 12 · see citing works
Engineering Cellulase Activity into Clostridium acetobutylicum
Fierobe, H., Mingardon, F., & Chanal, A. (2012) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/b978-0-12-415931-0.00016-1 · Cited by 12 · see citing works
Potential and Prospects of Biobutanol Production from Agricultural Residues
Behera, S., Selvaraju, K., Kumar, S., & M, J. A. B. (2019) · doi:10.1002/9781119459866.ch9 · Cited by 11 · see citing works
New host-vector system in solvent-producing Clostridium saccharoperbutylacetonicum strain N1-4
Nakayama, S., Irie, R., Kosaka, T., Matsuura, K., Yoshino, S., & Furukawa, K. (2007) · The Journal of General and Applied Microbiology · doi:10.2323/jgam.53.53 · Cited by 10 · see citing works
Wang, H., Liangyan, F., Wen, Q., Lin, J., & Liu, X. (2017) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-017-8116-9 · Cited by 9 · see citing works
Grosse-Honebrink, A., Little, G. T., Bean, Z., Heldt, D., Cornock, R. H. M., Winzer, K., Minton, N. P., Green, E., & Zhang, Y. (2021) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2021.659895 · Cited by 8 · see citing works
Kubiak, A. M., Claessen, L., Zhang, Y., Khazaie, K., & Bailey, T. S. (2023) · Frontiers in Immunology · doi:10.3389/fimmu.2023.1241632 · Cited by 8 · see citing works
A toolbox for genetic manipulation in intestinal Clostridium symbiosum
Yang, P., Tian, J., Zhang, L., Zhang, H., Yang, G., Ren, Y., Fang, J., Gu, Y., & Jiang, W. (2023) · Synthetic and Systems Biotechnology · doi:10.1016/j.synbio.2023.12.005 · Cited by 7 · see citing works
Carter, G. P., Minton, N. P., Davis, I. J., & Young, M. (2005) · CRC Press eBooks · doi:10.1201/9780203489819.ch3 · Cited by 7 · see citing works
Lessons from Clostridial Genetics: Toward Engineering Acetogenic Bacteria
Lee, J. (2021) · Biotechnology and Bioprocess Engineering · doi:10.1007/s12257-021-0062-9 · Cited by 6 · see citing works
New Tools for the Genetic Modification of Industrial Clostridia
Schwarz, K. M., Zhang, Y., Kuit, W., Ehsaan, M., Kovács, K., Winzer, K., & Minton, N. P. (2015) · Elsevier eBooks · doi:10.1016/b978-0-444-59592-8.00013-0 · Cited by 6 · see citing works
Oh, Y. H., Eom, G. T., Kang, K. H., Joo, J. C., Jang, Y.-A., Choi, J. W., Song, B. K., Lee, S. H., & Park, S. J. (2016) · Bioprocess and Biosystems Engineering · doi:10.1007/s00449-016-1537-5 · Cited by 5 · see citing works
Engineering Clostridium ljungdahlii for efficient 3-hydroxybutyrate production from one-carbon gases
Wu, Y., Ye, W., Li, R., Jia, D., Zhu, X., Jiang, W., & Gu, Y. (2025) · Green Synthesis and Catalysis · doi:10.1016/j.gresc.2025.01.001 · Cited by 4 · see citing works
Boudignon, E., Foulquier, C., & Soucaille, P. (2023) · Microorganisms · doi:10.3390/microorganisms11112696 · Cited by 4 · see citing works
Self-citationGenetic sensor-regulators functional in Clostridia
Han, S., Kim, Y., Karanjikar, M., San, K., & Bennett, G. N. (2020) · Journal of Industrial Microbiology & Biotechnology · doi:10.1007/s10295-020-02303-6 · Cited by 2 · see citing works
Genetic Manipulation ofClostridium
Bradshaw, M., & Johnson, E. A. (2014) · ASM Press eBooks · doi:10.1128/9781555816827.ch17 · Cited by 2 · see citing works
Expression of Recombinant Clostridial Neurotoxin by C. tetani
Gregg, B. M., Gupta, S., Tepp, W. H., & Pellett, S. (2024) · Microorganisms · doi:10.3390/microorganisms12122611 · Cited by 1 · see citing works
Engineering the Metabolic Profile of Clostridium cellulolyticum with Genomic DNA Libraries
Freedman, B. G., Lee, P. W., & Senger, R. S. (2023) · Fermentation · doi:10.3390/fermentation9070605 · Cited by 1 · see citing works
Schöllkopf, A. I., de Almeida, L. M. M., Krammer, K., Rivero, C. G., Liebl, W., & Ehrenreich, A. (2025) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-025-13404-6 · Cited by 1 · see citing works
Metabolic Engineering and the Synthetic Biology Toolbox forClostridium
Joseph, R. C., Kelley, S. Q., Kim, N. M., & Sandoval, N. R. (2021) · Metabolic Engineering · doi:10.1002/9783527823468.ch16 · Cited by 1 · see citing works
Characterisation and functional analysis of the putative agr system in Clostridium acetobutylicum
Scott, J. (2012) · UPT. Syiah Kuala University Library (Syiah Kuala University)
Demuez, M. (2007) · HAL · Cited by 1 · see citing works
Development of a Gene Expression Reporter System for Clostridium beijerinckii
Lee, S. H., Eom, G. T., Choi, M., Kang, K. H., Kang, S.-H., Park, S. J., & Song, B. K. (2010)
Hassan Mahmoud, M. A.-R. (2014) · OPen Access Repositorium der Universität Ulm (OPARU) (Ulm University) · doi:10.18725/oparu-3312
Cuív, P. Ó., Burman, S., Pottenger, S., & Morrison, M. (2016) · Springer eBooks · doi:10.1007/978-3-319-46326-1_3 · Cited by 1 · see citing works
Tollin, C. J. (2012) · VTechWorks (Virginia Tech)
Metabolism and cell-to-cell interactions of anaerobic syntrophic Clostridia co-cultures
Streett, H. (2025) · Library, Museums and Press - UDSpace (University of Delaware) · doi:10.58088/pe3w-rj36
9 Natural and Synthetic Gas for Productions of Liquid Fuels and Their Additives
Shah, Y. T. (2017) · CRC Press eBooks · doi:10.1201/9781315302355-10
- Authors
- Girbal, L., Mortier-Barrière, I., Raynaud, F., Rouanet, C., Croux, C., & Soucaille, P.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- CRT/CRITT-Bioindustries, INSA, DGBA, 31077 Toulouse cedex 4, France
- Centre de Bioingénierie Gilbert Durand, Laboratoire de Biotechnologie-Bioprocédés, UMR CNRS 5504, UMR INRA 792, INSA
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2003-08
- Volume
- 69
- Issue
- 8
- Pages
- 4985-4988
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- PMC
- PMC169153
- OpenAlex
- W2136479223
- Cited by
- 74
- References
- 24
- Citations by year
- 2025: 4 · 2024: 1 · 2023: 4 · 2022: 1 · 2021: 5 · 2020: 1 · 2019: 5 · 2018: 4 · 2017: 4 · 2016: 7 · 2015: 6 · 2014: 7 · 2013: 1 · 2012: 5
- FWCI
- 0.46
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Applied and Environmental Microbiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene Regulatory Network Analysis
- Keywords
- Clostridium acetobutylicum, gusA, beta-glucuronidase, acidogenesis, solventogenesis
- MeSH terms
- Base Sequence, Clostridium, Glucuronidase, Hydrogenase, Iron-Sulfur Proteins, Molecular Sequence Data, Promoter Regions, Genetic, Repressor Proteins, Transcription, Genetic, Gene Expression Regulation, Bacterial, Genes, Reporter
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
