The DOI matches its Crossref record; 32 of the 32 references it cites were found in a registry or an index.
Clostridium difficile toxin B is more potent than toxin A in damaging human colonic epithelium in vitro.
Open accessAbstract
Toxin A but not toxin B, appears to mediate intestinal damage in animal models of Clostridium difficile enteritis. The purpose of this study was to investigate the electrophysiologic and morphologic effects of purified C. difficile toxins A and B on human colonic mucosa in Ussing chambers. Luminal exposure of tissues to 16-65 nM of toxin A and 0.2-29 nM of toxin B for 5 h caused dose-dependent epithelial damage. Potential difference, short-circuit current and resistance decreased by 76, 58, and 46%, respectively, with 32 nM of toxin A and by 76, 55, and 47%, respectively, with 3 nM of toxin B, when compared with baseline (P < 0.05). 3 nM of toxin A did not cause electrophysiologic changes. Permeability to [3H]mannitol increased 16-fold after exposure to 32 nM of toxin A and to 3 nM of toxin B when compared with controls (P < 0.05). Light and scanning electron microscopy after exposure to either toxin revealed patchy damage and exfoliation of superficial epithelial cells, while crypt epithelium remained intact. Fluorescent microscopy of phalloidin-stained sections showed that both toxins caused disruption and condensation of cellular F-actin. Our results demonstrate that the human colon is approximately 10 times more sensitive to the damaging effects of toxin B than toxin A, suggesting that toxin B may be more important than toxin A in the pathogenesis of C. difficile colitis in man.
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Cites
Antibiotic-associated pseudomembranous colitis due to toxin-producing clostridia
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Role of Clostridium difficile in antibiotic-associated pseudomembranous colitis
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Self-citationPurification and characterization of toxins A and B of Clostridium difficile
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Self-citationMolecular characterization of the Clostridium difficile toxin A gene
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Biological activities of toxins A and B of Clostridium difficile
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Cited by
Clostridium difficile Infection
Leffler, D. A., & Lamont, J. T. (2015) · New England Journal of Medicine · doi:10.1056/nejmra1403772 · Cited by 1,450
Self-citationClostridium difficile infection: new developments in epidemiology and pathogenesis
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Asymptomatic Carriage of Clostridium difficile and Serum Levels of IgG Antibody against Toxin A
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Clostridium difficile infection
Smits, W. K., Lyras, D., Lacy, D. B., Wilcox, M. H., & Kuijper, E. J. (2016) · Nature Reviews Disease Primers · doi:10.1038/nrdp.2016.20 · Cited by 853
Enteric bacterial toxins: mechanisms of action and linkage to intestinal secretion
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Metadata
- Authors
- Riegler, M., Sedivy, R., Pothoulakis, C., Hamilton, G., Zacherl, J., Bischof, G., Cosentini, E., Feil, W., Schiessel, R., & LaMont, J. T.
- Venue
- Journal of Clinical Investigation
- Published
- 1995-05-01
- Volume
- 95
- Issue
- 5
- Pages
- 2004-2011
- ISSN
- 0021-9738 (ISSN-L)
- OpenAlex
- W2003370562
- Cited by
- 340
- References
- 32
- FWCI
- 15.78
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: Clostridium difficile toxin B is more potent than toxin A in damaging human colonic epithelium in vitro. https://app.cochat.ai/papers/10.1172/jci117885
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- Riegler, M., Sedivy, R., Pothoulakis, C., Hamilton, G., Zacherl, J., Bischof, G., Cosentini, E., Feil, W., Schiessel, R., & LaMont, J. T. (1995). Clostridium difficile toxin B is more potent than toxin A in damaging human colonic epithelium in vitro. Journal of Clinical Investigation. https://doi.org/10.1172/jci117885
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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