The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
Sau1: a Novel Lineage-Specific Type I Restriction-Modification System That Blocks Horizontal Gene Transfer into Staphylococcus aureus and between S. aureus Isolates of Different Lineages
Abstract
The Sau1 type I restriction-modification system is found on the chromosome of all nine sequenced strains of Staphylococcus aureus and includes a single hsdR (restriction) gene and two copies of hsdM (modification) and hsdS (sequence specificity) genes. The strain S. aureus RN4220 is a vital intermediate for laboratory S. aureus manipulation, as it can accept plasmid DNA from Escherichia coli . We show that it carries a mutation in the sau1hsdR gene and that complementation restored a nontransformable phenotype. Sau1 was also responsible for reduced conjugative transfer from enterococci, a model of vancomycin resistance transfer. This may explain why only four vancomycin-resistant S. aureus strains have been identified despite substantial selective pressure in the clinical setting. Using a multistrain S. aureus microarray, we show that the two copies of sequence specificity genes ( sau1hsdS1 and sau1hsdS2 ) vary substantially between isolates and that the variation corresponds to the 10 dominant S. aureus lineages. Thus, RN4220 complemented with sau1hsdR was resistant to bacteriophage lysis but only if the phage was grown on S. aureus of a different lineage. Similarly, it could be transduced with DNA from its own lineage but not with the phage grown on different S. aureus lineages. Therefore, we propose that Sau1 is the major mechanism for blocking transfer of resistance genes and other mobile genetic elements into S. aureus isolates from other species, as well as for controlling the spread of resistance genes between isolates of different S. aureus lineages. Blocking Sau1 should also allow genetic manipulation of clinical strains of S. aureus .
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Diep, B. A., Gill, S. R., Chang, R. F., Phan, T. H., Chen, J. H., Davidson, M. G., Lin, F., Lin, J., Carleton, H. A., Mongodin, E. F., et al. (2006) · The Lancet · doi:10.1016/s0140-6736(06)68231-7 · Cited by 1,633
The toxic shock syndrome exotoxin structural gene is not detectably transmitted by a prophage
Kreiswirth, B. N., Löfdahl, S., Betley, M. J., O'Reilly, M., Schlievert, P. M., Bergdoll, M. S., & Novick, R. P. (1983) · Nature · doi:10.1038/305709a0 · Cited by 1,326
Ito, T., Katayama, Y., Asada, K., Mori, N., Tsutsumimoto, K., Tiensasitorn, C., & Hiramatsu, K. (2001) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.45.5.1323-1336.2001 · Cited by 963
Arnaud, M., Chastanet, A., & Débarbouillé, M. (2004) · Applied and Environmental Microbiology · doi:10.1128/aem.70.11.6887-6891.2004 · Cited by 944
Holden, M. T. G., Feil, E. J., Lindsay, J. A., Peacock, S. J., Day, N. P. J., Enright, M. C., Foster, T. J., Moore, C. E., Hurst, L., Atkin, R., et al. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0402521101 · Cited by 892
Self-citationGenetic Analysis of a High-Level Vancomycin-Resistant Isolate of Staphylococcus aureus
Weigel, L. M., Clewell, D. B., Gill, S. R., Clark, N. C., McDougal, L. K., Flannagan, S. E., Kolonay, J. F., Shetty, J., Killgore, G. E., & Tenover, F. C. (2003) · Science · doi:10.1126/science.1090956 · Cited by 889
A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes
Roberts, R. J. (2003) · Nucleic Acids Research · doi:10.1093/nar/gkg274 · Cited by 755
Properties of a cryptic high-frequency transducing phage in Staphylococcus aureus
Novick, R. (1967) · Virology · doi:10.1016/0042-6822(67)90105-5 · Cited by 741
How Clonal Is Staphylococcus aureus ?
Feil, E. J., Cooper, J. E., Grundmann, H., Robinson, D. A., Enright, M. C., Berendt, T., Peacock, S. J., Smith, J. M., Murphy, M., Spratt, B. G., et al. (2003) · Journal of Bacteriology · doi:10.1128/jb.185.11.3307-3316.2003 · Cited by 618
Dufour, P., Gillet, Y., Bes, M., Lina, G., Vandenesch, F., Floret, D., Etienne, J., & Richet, H. (2002) · Clinical Infectious Diseases · doi:10.1086/342576 · Cited by 513
Cited by
Methicillin-Resistant Staphylococcus aureus: Molecular Characterization, Evolution, and Epidemiology
Lakhundi, S., & Zhang, K. (2018) · Clinical Microbiology Reviews · doi:10.1128/cmr.00020-18 · Cited by 1,637
Food Poisoning and Staphylococcus aureus Enterotoxins
Argudín, M. Á., Mendoza, M. C., & Rodicio, M. R. (2010) · Toxins · doi:10.3390/toxins2071751 · Cited by 1,161
Mechanisms of Methicillin Resistance in Staphylococcus aureus
Peacock, S. J., & Paterson, G. K. (2015) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-060614-034516 · Cited by 656
Stefani, S., Chung, D. R., Lindsay, J. A., Friedrich, A. W., Kearns, A. M., Westh, H., & MacKenzie, F. M. (2012) · International Journal of Antimicrobial Agents · doi:10.1016/j.ijantimicag.2011.09.030 · Cited by 590
Self-citationMobile genetic elements of Staphylococcus aureus
Malachowa, N., & DeLeo, F. R. (2010) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-010-0389-4 · Cited by 560
Metadata
- Authors
- Waldron, D. E., & Lindsay, J. A.
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 2006-08
- Volume
- 188
- Issue
- 15
- Pages
- 5578-5585
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- OpenAlex
- W2130920842
- Cited by
- 300
- References
- 27
- FWCI
- 7.07
- Type
- journal article
- Language
- en
- Open access
- closed
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- Waldron, D. E., & Lindsay, J. A. (2006). Sau1: a Novel Lineage-Specific Type I Restriction-Modification System That Blocks Horizontal Gene Transfer into Staphylococcus aureus and between S. aureus Isolates of Different Lineages. Journal of Bacteriology. https://doi.org/10.1128/jb.00418-06
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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