Repair of DNA Double-Strand Breaks following UV Damage in Three Sulfolobussolfataricus Strains

Rolfsmeier, Michael L.; Laughery, Marian F.; Haseltine, Cynthia A.

doi:10.1128/jb.00667-10

Cited by 42DNA Repair MechanismsBacterial Genetics and BiotechnologyFungal and yeast genetics research

Repair of DNA Double-Strand Breaks following UV Damage in Three Sulfolobussolfataricus Strains

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Rolfsmeier, Michael L., Laughery, Marian F., Haseltine, Cynthia A.·2010·Journal of Bacteriology

DOI 10.1128/jb.00667-10PMC PMC2944543

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Abstract

DNA damage repair mechanisms have been most thoroughly explored in the eubacterial and eukaryotic branches of life. The methods by which members of the archaeal branch repair DNA are significantly less well understood but have been gaining increasing attention. In particular, the approaches employed by hyperthermophilic archaea have been a general source of interest, since these organisms thrive under conditions that likely lead to constant chromosomal damage. In this work we have characterized the responses of three Sulfolobus solfataricus strains to UV-C irradiation, which often results in double-strand break formation. We examined S. solfataricus strain P2 obtained from two different sources and S. solfataricus strain 98/2, a popular strain for site-directed mutation by homologous recombination. Cellular recovery, as determined by survival curves and the ability to return to growth after irradiation, was found to be strain specific and differed depending on the dose applied. Chromosomal damage was directly visualized using pulsed-field gel electrophoresis and demonstrated repair rate variations among the strains following UV-C irradiation-induced double-strand breaks. Several genes involved in double-strand break repair were found to be significantly upregulated after UV-C irradiation. Transcript abundance levels and temporal expression patterns for double-strand break repair genes were also distinct for each strain, indicating that these Sulfolobus solfataricus strains have differential responses to UV-C-induced DNA double-strand break damage.

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Cells evolved complex ATM and ATR signaling events that lead to activation of the two basic DSB repair mechanisms.
“To ensure that such a detrimental damage will be repaired, cells evolved complex ATM and ATR signaling events leading to activation of the two basic DSB repair mechanisms compared in Table 2 [115,116,117].”
From Focus on UV-Induced DNA Damage and Repair—Disease Relevance and Protective Strategies (2020) · cites this paper as [116]

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Authors: Rolfsmeier, M. L., Laughery, M. F., & Haseltine, C. A.
Affiliations: Washington State University (US)
ORCID: Rolfsmeier, Michael L.
Haseltine, Cynthia A.
Venue: Journal of Bacteriology
Publisher: American Society for Microbiology
Published: 2010-10
Volume: 192
Issue: 19
Pages: 4954-4962
ISSN: 0021-9193 (ISSN-L)
1098-5530
DOI: 10.1128/jb.00667-10
PMC: PMC2944543
OpenAlex: W2061089401
Cited by: 42
References: 48
Citations by year: 2026: 2 · 2025: 2 · 2024: 1 · 2023: 2 · 2022: 5 · 2021: 1 · 2020: 6 · 2018: 4 · 2017: 1 · 2016: 2 · 2015: 5 · 2014: 1 · 2013: 2 · 2012: 2
FWCI: 1.30
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Licence: journals.asm.org/non-commercial-tdm-license (tdm)
Locations: Journal of Bacteriology (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Funders: National Science Foundation (0951125)
American Cancer Society
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
Keywords: DNA double-strand break repair, UV-C irradiation, Sulfolobus solfataricus, hyperthermophilic archaea, chromosomal damage, gene expression response, survival curves, pulsed-field gel electrophoresis, site-directed mutagenesis
MeSH terms: Chromosomes, Bacterial, DNA Repair, Ultraviolet Rays, Mutagenesis, Site-Directed, Electrophoresis, Gel, Pulsed-Field, Reverse Transcriptase Polymerase Chain Reaction, Sulfolobus solfataricus, DNA Breaks, Double-Stranded

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Repair of DNA Double-Strand Breaks following UV Damage in Three Sulfolobussolfataricus Strains

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