The DOI matches its Crossref record; 39 of the 39 references it cites were found in a registry or an index.
Identification and Characterization of an Acinetobacter baumannii Biofilm-Associated Protein
Open accessAbstract
We have identified a homologue to the staphylococcal biofilm-associated protein (Bap) in a bloodstream isolate of Acinetobacter baumannii . The fully sequenced open reading frame is 25,863 bp and encodes a protein with a predicted molecular mass of 854 kDa. Analysis of the nucleotide sequence reveals a repetitive structure consistent with bacterial cell surface adhesins. Bap-specific monoclonal antibody (MAb) 6E3 was generated to an epitope conserved among 41% of A. baumannii strains isolated during a recent outbreak in the U.S. military health care system. Flow cytometry confirms that the MAb 6E3 epitope is surface exposed. Random transposon mutagenesis was used to generate A. baumannii bap1302 ::EZ-Tn 5 , a mutant negative for surface reactivity to MAb 6E3 in which the transposon disrupts the coding sequence of bap . Time course confocal laser scanning microscopy and three-dimensional image analysis of actively growing biofilms demonstrates that this mutant is unable to sustain biofilm thickness and volume, suggesting a role for Bap in supporting the development of the mature biofilm structure. This is the first identification of a specific cell surface protein directly involved in biofilm formation by A. baumannii and suggests that Bap is involved in intercellular adhesion within the mature biofilm.
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Cited by
Russo, T. A., Luke, N. R., Beanan, J. M., Olson, R., Sauberan, S. L., MacDonald, U., Schultz, L. W., Umland, T. C., & Campagnari, A. A. (2010) · Infection and Immunity · doi:10.1128/iai.00366-10 · Cited by 324
Self-citationAdhesins Involved in Attachment to Abiotic Surfaces by Gram-Negative Bacteria
Berne, C., Ducret, A., Hardy, G. G., & Brun, Y. V. (2015) · Microbiology Spectrum · doi:10.1128/microbiolspec.mb-0018-2015 · Cited by 323
Isolation and Characterization of an Autoinducer Synthase from Acinetobacter baumannii
Niu, C., Clemmer, K. M., Bonomo, R. A., & Rather, P. N. (2008) · Journal of Bacteriology · doi:10.1128/jb.01929-07 · Cited by 279
Luke, N. R., Sauberan, S. L., Russo, T. A., Beanan, J. M., Olson, R., Loehfelm, T. W., Cox, A. D., St. Michael, F., Vinogradov, E. V., & Campagnari, A. A. (2010) · Infection and Immunity · doi:10.1128/iai.00016-10 · Cited by 114
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Metadata
- Authors
- Loehfelm, T. W., Luke, N. R., & Campagnari, A. A.
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 2008-02
- Volume
- 190
- Issue
- 3
- Pages
- 1036-1044
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- OpenAlex
- W2009912226
- Cited by
- 279
- References
- 39
- FWCI
- 2.39
- Type
- journal article
- Language
- en
- Open access
- green
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