CoChat
Sign Up
Cited by 50Bacterial Infections and VaccinesBiosensors and Analytical DetectionBacterial Identification and Susceptibility Testing

Loop-Mediated Isothermal Amplification Assay for Detection of Haemophilus influenzae Type b in Cerebrospinal Fluid

Open access
Kim, Dong Wook, Kilgore, Paul Evan, Kim, Eun Jin, Kim, Soon Ae, Anh, Dang Duc, Seki, Mitsuko·2011·Journal of Clinical Microbiology
DOI 10.1128/jcm.00515-11PMC PMC3187296
Open this paper in CoChat — free account

Abstract

Haemophilus influenzae type b (Hib) is one of the leading causes of meningitis in developing countries. To establish and evaluate a novel loop-mediated isothermal amplification (LAMP) assay for Hib, we designed a LAMP primer set targeting the Hib-specific capsulation locus. LAMP detected 10 copies of purified DNA in a 60-min reaction. This indicated that the detection limit of LAMP was >100-fold lower than the detection limits of both a PCR for the detection of bexA and a nested PCR for Hib (Hib PCR). No H. influenzae, other than Hib or control bacteria, was detected. Linear determination ranged from 10 to 1,000,000 microorganisms per reaction mixture using real-time turbidimetry. We evaluated the Hib LAMP assay using a set of 52 randomly selected cerebrospinal fluid (CSF) specimens obtained from children with suspected meningitis. For comparison, the CSF specimens were tested using a conventional Hib PCR assay. Hib was detected in 30 samples using LAMP and in 22 samples using the Hib PCR assay. The Hib PCR showed a clinical sensitivity of 73.3% and a clinical specificity of 100% relative to the Hib LAMP assay. These results suggest that further development and evaluation of the Hib LAMP will enhance the global diagnostic capability for Hib detection.

Integrity signals

Registry

Crossref · 10.1128/jcm.00515-11

Cites

23 of 23 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • The sensitivity of bexA PCR for Haemophilus influenzae ranged from 81% to 100% with a median of 93%, and specificity ranged from 81% to 100% with a median of 94%.

    “The sensitivity of bexA PCR ranged from 81% to 100% with a median of 93%, with a specificity ranging from 81% to 100% (median 94%) (54, 58, 65–68).”

    From Molecular diagnostics in cerebrospinal fluid for the diagnosis of central nervous system infections (2024) · cites this paper as [66]

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Related

Authors
Kim, D. W., Kilgore, P. E., Kim, E. J., Kim, S. A., Anh, D. D., & Seki, M.
Affiliations
  • International Vaccine Institute (KR)
  • National Institute of Hygiene and Epidemiology, Hanoi, Vietnam
  • Nihon University (JP)
Corresponding author
Seki, Mitsuko
ORCID
Venue
Journal of Clinical Microbiology
Publisher
American Society for Microbiology
Published
2011-10
Volume
49
Issue
10
Pages
3621-3626
ISSN
  • 0095-1137 (ISSN-L)
  • 1098-660X
DOI
10.1128/jcm.00515-11
PMC
PMC3187296
OpenAlex
W2091575535
Cited by
50
References
23
Citations by year
2026: 2 · 2025: 5 · 2024: 2 · 2023: 1 · 2022: 5 · 2021: 3 · 2020: 2 · 2019: 3 · 2018: 3 · 2017: 5 · 2016: 6 · 2015: 3 · 2014: 6 · 2013: 1 · 2012: 3
FWCI
2.97
Type
journal article
Language
en
Update notices
correction (2014-03-11)
Open access
Licence
journals.asm.org/non-commercial-tdm-license (tdm)
Locations
  • Journal of Clinical Microbiology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • The Royal Society of Chemistry’s Journals, Books and Databases (The Royal Society of Chemistry) (submittedVersion)
Funders
  • School of Dentistry, Nihon University
  • Japan Society for the Promotion of Science (22592348)
Topics
  • Life Sciences › Immunology and Microbiology › Microbiology › Bacterial Infections and Vaccines
  • Physical Sciences › Engineering › Biomedical Engineering › Biosensors and Analytical Detection
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Bacterial Identification and Susceptibility Testing
Keywords
Haemophilus influenzae type b, loop-mediated isothermal amplification, cerebrospinal fluid, meningitis, molecular diagnosis, sensitivity and specificity, turbidimetric detection
MeSH terms
Bacteriological Techniques, Cerebrospinal Fluid, Humans, Meningitis, Haemophilus, Sensitivity and Specificity, Temperature, Time Factors, DNA Primers, Haemophilus influenzae type b, Nucleic Acid Amplification Techniques, Molecular Diagnostic Techniques

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review