Loop-mediated isothermal amplification methods for diagnosis of bacterial meningitis
Open accessAbstract
The rapid, accurate, and efficient identification of an infectious disease is critical to ensure timely clinical treatment and prevention in public health settings. In 2015, meningitis caused by Streptococcus pneumoniae, Haemophilus influenzae, and Neisseria meningitidis was responsible for 379,200 (range: 322,700 to 444,700) deaths. Clinical features alone cannot determine whether bacterial meningitis is present; an analysis of cerebrospinal fluid (CSF) is essential. Loop-mediated isothermal amplification (LAMP) is a nucleic acid amplification method offering an alternative to polymerase chain reaction (PCR). LAMP-based assays for detection of three leading bacteria in CSF for diagnosis of meningitis have been established. The typing assays using LAMP for detection of meningococcal serogroups A, B, C, W, X, and Y as well as H. influenzae serotypes a, b, c, d, e, and f were launched. In comparative analysis of the meningitis pathogen assays, LAMP assays did not yield false negative results, and the detection rate of LAMP assays was superior compared with PCR or conventional culture methods. LAMP assays provide accurate and rapid test results to detect major bacterial meningitis pathogens. Accumulating evidence suggests that LAMP assays have the potential to provide urgently needed diagnostics for bacterial meningitis in resource-limited settings of both developed and developing countries.
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Delayed LPs are associated with increased mortality in ABM.
“Delayed LPs in particular have been shown to be associated with increased mortality in ABM.62”
From Community-acquired acute bacterial meningitis in adults: a clinical update (2019) · cites this paper as [62]
The Introduction says that prompt identification and CSF analysis are important for bacterial meningitis, but it does not discuss delayed lumbar puncture or its association with mortality. Searches for delayed lumbar puncture, LP, and mortality found no passage addressing that association. (LLM, conf=0.76)
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Methods for Rapid Diagnosis of Meningitis Etiology in Adults
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“A study, including 18 PCR-confirmed pneumococcal meningitis patients, showed that 100% of cases were also positive by LAMP targeting the lytA gene (59).”
Community-acquired acute bacterial meningitis in adults: a clinical update
Ellis, J., Luintel, A., Chandna, A., & Heyderman, R. S. (2019) · British Medical Bulletin · doi:10.1093/bmb/ldz023 · Cited by 12 · see citing works
- Authors
- Seki, M., Kilgore, P. E., Kim, E. J., Ohnishi, M., Hayakawa, S., & Kim, D. W.
- Affiliations
- Nihon University (JP)
- Eugene Applebaum College of Pharmacy and Health Sciences (US)
- Hanyang University (KR)
- National Institute of Infectious Diseases (JP)
- Corresponding author
- Seki, Mitsuko
- Kim, Dong Wook
- Venue
- Frontiers in Pediatrics
- Publisher
- Frontiers Media SA
- Published
- 2018-03-12
- Volume
- 6
- Pages
- 57-57
- ISSN
- 2296-2360 (ISSN-L)
- PMC
- PMC5857938
- OpenAlex
- W2794212109
- Cited by
- 60
- References
- 48
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 7 · 2023: 5 · 2022: 7 · 2021: 15 · 2020: 12 · 2019: 10 · 2018: 1
- FWCI
- 0.77
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Frontiers in Pediatrics (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Human Biology (submittedVersion, cc-by)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Japan Society for the Promotion of Science (Bilateral Open Partnership Joint Research Projects)
- National Research Foundation of Korea (NRF-2015R1A2A2A01007297, NRF-2015M3C9A2054024)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Biosensors and Analytical Detection
- Life Sciences › Immunology and Microbiology › Microbiology › Bacterial Infections and Vaccines
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Bacterial Identification and Susceptibility Testing
- Keywords
- loop-mediated isothermal amplification, bacterial meningitis, cerebrospinal fluid, Streptococcus pneumoniae, Haemophilus influenzae, Neisseria meningitidis, meningococcal serogroups, rapid molecular diagnostics, resource-limited settings
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